Polypeptides and immunizing compositions containing burkholderia polypeptides and methods of use

ABSTRACT

This disclosure provides isolated polypeptides isolatable from a  Burkholderia  spp., compositions that include one or more of the polypeptides, and methods for making and methods for using the polypeptides. This disclosure also provides antibody that specifically binds one or more of the polypeptides isolatable from a  Burkholderia  spp, compositions that include such antibody, and methods for using such antibody.

CROSS-REFERENCE TO RELATED APPLICATION

This application claims priority to U.S. Provisional Patent Application Ser. No. 61/922,504, filed Dec. 31, 2013, which is incorporated herein by reference.

BACKGROUND

Burkholderia is a genus of proteobacteria known for its pathogenic members: Burkholderia mallei, responsible for glanders, a disease that occurs mostly in horses and related animals; Burkholderia pseudomallei, causative agent of melioidosis; and the Burkholderia cepacia complex, which includes pathogens that are involved in pulmonary infections of people with cystic fibrosis (CF). The Burkholderia genus name refers to a group of common gram-negative, motile, obligately aerobic rod-shaped bacteria including animal, human, and plant pathogens. Due to their antibiotic resistance and the high mortality rate from their associated diseases, Burkholderia mallei and Burkholderia pseudomallei are considered to be potential biological warfare agents, with livestock and humans as potential targets.

Humans and animals are believed to acquire Burkholderia infection by inhalation of contaminated dust or water droplets, ingestion of contaminated water, and contact with contaminated soil, especially through skin abrasions.

Many non-human animal species can be susceptible to melioidosis caused by Burkholderia pseudomallei, including many livestock and/or companion animal species such as, for example, sheep, goats, horses, swine, cattle, dogs, and cats.

In the absence of treatment with appropriate antibiotics, the septicemic form of melioidosis has a mortality rate that exceeds 90%. With appropriate antibiotic treatment, the mortality rate is about 10% for uncomplicated cases but up to 80% for cases with bacteremia or severe sepsis. Because of its severe course of infection, aerosol infectivity, and worldwide availability, B. pseudomallei is identified as a potential agent of biological warfare or bioterrorism and is listed on the Centers for Disease Control list as a Category B bioterrorism agent. There is currently no vaccine and the organism is often refractory to antibiotic therapy, especially after it is established in a host.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1. SDS-PAGE (10%) analysis of polypeptides isolated from B. thailandensis grown in either iron-rich (Fe, Lane 2) or iron-restricted (DP, Lane 3) medium. Lane 1: molecular weight standard.

FIG. 2. Two-dimensional gel electrophoresis analysis of extracts from B. thailandensis grown in either (A) iron-rich or (B) iron-depleted medium.

FIG. 3. Data from a vaccine trial in A/J mice. Kaplan-Meier survival curve of A/J mice immunized twice with varying doses of vaccine (50 ng/dose, 100 ng/dose, or 300 ng/dose) or a placebo (adjuvant only) and challenged intratracheally with B. thailandensis.

FIG. 4. Data from a vaccine trial in BALB/c mice. Kaplan-Meier survival curve of BALB/c mice immunized twice with varying doses of vaccine (50 ng/dose, 100 ng/dose, or 300 ng/dose) or a placebo (adjuvant only) and challenged intratracheally with B. thailandensis.

FIGS. 5A-B. Protein conservation across Burkholderia strains and species. (A) Heat map showing the percent amino acid identity for 25 receptor proteins was determined in 12 strains of B. pseudomallei, seven strains of B. mallei, three strains of B. thailandensis, six strains of B. cenocepacia, and five strains of B. multivorans using B. pseudomallei K96243 as the query. (B) Heat map showing the percent amino acid identity for 25 receptor proteins was determined in 12 strains of B. pseudomallei, seven strains of B. mallei, three strains of B. thailandensis, six strains of B. cenocepacia, and five strains of B. multivorans using B. thailandensis E264 as the query.

FIG. 6A-C. Multiple cross-species sequence alignment for the Burkholderia spp. polypeptides across five Burkholderia species: B. thailandensis E264, B. pseudomallei K96243, B. mallei NCTC10229, B. cenocepacia AU1054, and B. multivorans ATCC17616. (A) SEQ ID NO:1, (B) SEQ ID NO:2, and (C) SEQ ID NO:9. (* indicates identity).

FIG. 7. Antibody titers to individual recombinant Burkholderia iron-regulated polypeptides in vaccinated mice.

DETAILED DESCRIPTION OF EXEMPLARY EMBODIMENTS

In one aspect, this disclosure provides polypeptides and compositions including polypeptides. As used herein, “polypeptide” refers to a polymer of amino acids linked by peptide bonds. Thus, for example, the terms peptide, oligopeptide, protein, and enzyme are included within the definition of polypeptide. This term also includes polypeptides that may include one or more post-expression modifications of the polypeptide such as, for example, a glycosylation, an acetylation, a phosphorylation, and the like. The term polypeptide does not connote a specific length of a polymer of amino acids. A polypeptide may be isolatable directly from a natural source or can be prepared with the aid of recombinant, enzymatic, or chemical techniques. In the case of a polypeptide that is naturally occurring, such a polypeptide is typically isolated.

An “isolated” polypeptide is one that has been removed from its natural environment. For instance, an isolated polypeptide is a polypeptide that has been removed from the cytoplasm or from the membrane of a cell, and many of the polypeptides, nucleic acids, and other cellular material of its natural environment are no longer present.

A polypeptide characterized as “isolatable” from a particular source is a polypeptide that, under appropriate conditions, is produced by the identified source, although the polypeptide may be obtained from alternate sources using, for example, conventional recombinant, chemical, or enzymatic techniques. Thus, characterizing a polypeptide as “isolatable” from a particular source does not imply any specific source from which the polypeptide must be obtained or any particular conditions or processes under which the polypeptide must be obtained.

A “purified” polypeptide is one that is at least 60% free, preferably at least 75% free, and most preferably at least 90% free from other components with which they are naturally associated. Polypeptides that are produced outside the organism in which they naturally occur, e.g., through chemical or recombinant means, are considered to be isolated and purified by definition, since they were never present in a natural environment.

As used herein, a “polypeptide fragment” refers to a portion of a polypeptide that results from digestion of a polypeptide with a protease.

Unless otherwise specified, “a,” “an,” “the,” and “at least one” are used interchangeably and mean one or more than one. The terms “comprises” and variations thereof do not have a limiting meaning where these terms appear in the description and claims. Also, the term “and/or” means one or all of the listed elements or a combination of any two or more of the listed elements.

Generally, a polypeptide may be characterized by molecular weight, mass fingerprint, amino acid sequence, nucleic acid that encodes the polypeptide, immunological activity, or any combination of two or more such characteristics. The molecular weight of a polypeptide, typically expressed in kilodaltons (kDa), can be determined using routine methods including, for instance, gel filtration, gel electrophoresis including sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE), capillary electrophoresis, mass spectrometry, liquid chromatography (including HPLC), and calculating the molecular weight from an observed or predicted amino acid sequence. Unless indicated otherwise, molecular weight refers to molecular weight as determined by resolving a polypeptide using an SDS polyacrylamide gel having a stacking gel of about 4% and a resolving gel of about 10% under reducing and denaturing conditions.

As used herein, a “mass fingerprint” refers to a population of polypeptide fragments obtained from a polypeptide after digestion with a protease. Often, a mass fingerprint can be generated by digesting a polypeptide with trypsin. In principle, however, a mass fingerprint may be generated by digesting the polypeptide with any suitable protease. Typically, the polypeptide fragments resulting from a digestion are analyzed using a mass spectrometric method. Each polypeptide fragment is characterized by a mass, or by a mass (m) to charge (z) ratio, which is referred to as an “m/z ratio” or an “m/z value.” Methods for generating a mass fingerprint of a polypeptide are routine. An example of such a method is disclosed in Example 3.

The polypeptides described herein may be metal-regulated. As used herein, a “metal-regulated polypeptide” is a polypeptide that is expressed by a microbe at a greater level when the microbe is grown in low metal conditions compared to when the same microbe is grown in high metal conditions. Low metal and high metal conditions are described herein. For instance, certain metal-regulated polypeptides produced by Burkholderia spp. are not expressed at detectable levels during growth of the microbe in high metal conditions but are expressed at detectable levels during growth in low metal conditions.

Examples of metal-regulated polypeptides isolatable from B. thailandensis after growth in low iron conditions include metal-regulated polypeptides having molecular weights of 88 kDa, 84 kDa, 83 kDa, 81 kDa, 58 kDa, 56 kDa, 55 kDa, 44 kDa, 43 kDa, 42 kDa, 27 kDa, 24 kDa, or 19 kDa.

Additional examples of metal-regulated polypeptides include recombinantly-produced versions of polypeptides described herein. A recombinantly-produced polypeptide may include the entire amino acid sequence translatable from an mRNA transcript. Alternatively, a recombinantly-produced metal-regulated polypeptide can include a fragment or portion of the entire translatable amino acid sequence. For example, a recombinantly-produced metal-regulated polypeptide may lack a cleavable sequence at either terminal of the polypeptide—e.g., a cleavable signal sequence at the amino terminal of the polypeptide.

Thus, a metal-regulated polypeptide can be a polypeptide that includes the amino acid sequence depicted in, for example, SEQ ID NO:6, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:1, SEQ ID NO:7, SEQ ID NO:16, SEQ ID NO:22, SEQ ID NO:2, SEQ ID NO:13, SEQ ID NO:25, SEQ ID NO:21, SEQ ID NO:9, SEQ ID NO:11, or SEQ ID NO:10.

This disclosure also describes certain polypeptides that are not metal-regulated. Such polypeptides are expressed in the presence of a metal ion such as, for example, in the presence of ferric chloride, and also expressed when grown in low iron conditions. Examples of such polypeptides isolatable from B. thailandensis have molecular weights of 55 kDa, 40 kDa, 39 kDa, or 19 kDa.

Additional examples of polypeptides that are not metal-regulated include recombinantly-produced versions of polypeptides described herein. A recombinantly-produced polypeptide may include the entire amino acid sequence translatable from an mRNA transcript. Alternatively, a recombinantly-produced non-metal-regulated polypeptide can include a fragment or portion of the entire translatable amino acid sequence. For example, a recombinantly-produced non-metal-regulated polypeptide may lack a cleavable sequence at either terminal of the polypeptide—e.g., a cleavable signal sequence at the amino terminal of the polypeptide.

Thus, a polypeptide that is not metal-regulated can be a polypeptide that includes the amino acid sequence depicted in, for example, SEQ ID NO:15, SEQ ID NO:12, SEQ ID NO:14, and SEQ ID NO:18.

This disclosure also describes certain polypeptides whose metal-regulation has not been established with certainty. Such polypeptides may have been detected in the extract when grown in low iron or in the presence of iron. Alternatively, they may have been identified through bioinformatics analysis using structural similarity to known iron receptor proteins or functional roles in iron acquisition or storage. Examples of such polypeptides isolatable from B. thailandensis have molecular weights of 85 kDa, 81 kDa, 78 kDa, 55 kDa, 36 kDa, or 8.5 kDa.

Additional examples of polypeptides whose metal regulation is uncertain include recombinantly-produced versions of polypeptides described herein. A recombinantly-produced polypeptide may include the entire amino acid sequence translatable from an mRNA transcript. Alternatively, a recombinantly-produced polypeptide whose metal regulation is uncertain can include a fragment or portion of the entire translatable amino acid sequence. For example, a recombinantly-produced polypeptide whose metal regulation is uncertain may lack a cleavable sequence at either terminal of the polypeptide—e.g., a cleavable signal sequence at the amino terminal of the polypeptide.

Thus, a polypeptide whose metal regulation is uncertain can be a polypeptide that includes the amino acid sequence depicted in, for example, SEQ ID NO:23, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:24, SEQ ID NO:17, SEQ ID NO:20, and SEQ ID NO:19.

Whether a polypeptide is a metal-regulated polypeptide or a non-metal-regulated polypeptide can be determined by methods useful for comparing the presence of polypeptides, including, for example, gel filtration, gel electrophoresis including sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), capillary electrophoresis, mass spectrometry, isobaric tags for relative and absolute quantification (iTRAQ), and liquid chromatography including HPLC. Separate cultures of a microbe can be grown under high metal conditions and under low metal conditions, polypeptides may be isolated as described herein, and the polypeptides present in each culture can be resolved and compared. Typically, an equal amount of polypeptides from each culture is used. Preferably, the polypeptides can be resolved using an SDS polyacrylamide gel having a stacking gel of about 4% and a resolving gel of about 10% under reducing and denaturing conditions. For instance, 30 micrograms (μg) of total polypeptide from each culture may be used and loaded into wells of a gel. After running the gel and staining the polypeptides with Coomassie Brilliant Blue, the two lanes can be compared. When determining whether a polypeptide is or is not expressed at a detectable level, 30 μg of total polypeptide from a culture is resolved on an SDS-PAGE gel and stained with Coomassie Brilliant Blue using methods known in the art. A polypeptide that can be visualized by eye is considered to be expressed at a detectable level, while a polypeptide that cannot be visualized by eye is considered to not be expressed at a detectable level.

Alternatively, whether a polypeptide is a metal-regulated polypeptide or a non-metal-regulated polypeptide can be determined using microarray-based gene expression analysis. Separate cultures of a microbe can be grown under high metal conditions and under low metal conditions, RNA can be extracted from cells of each culture, and differences in RNA expression in cells grown in high metal conditions versus RNA expression in cells grown in low metal conditions can be detected and compared. For example, labeled cDNA can be prepared from 8-10 μg of bacterial RNA using established protocols. The labeled cDNA can be applied to a microarray of the Burkholderia spp. genome. Such microarrays are commercially available and gene expression using such arrays is routine.

The polypeptides described herein may have immunological activity. “Immunological activity” refers to the ability of a polypeptide to elicit an immunological response in an animal. An immunological response to a polypeptide is the development in an animal of a cellular and/or antibody-mediated immune response to the polypeptide. Usually, an immunological response includes but is not limited to one or more of the following effects: the production of antibodies, B cells, helper T cells, suppressor T cells, and/or cytotoxic T cells, directed to an epitope or epitopes of the polypeptide. “Epitope” refers to the site on an antigen to which specific B cells and/or T cells respond so that antibody is produced. The immunological activity may be protective. “Protective immunological activity” refers to the ability of a polypeptide to elicit an immunological response in an animal that inhibits or limits infection by Burkholderia spp. such as, for example, B. thailandensis, B. mallei, B. pseudomallei, B. cenocepacia, or B. multivorans. Whether a polypeptide has protective immunological activity can be determined by methods known in the art such as, for example, methods described in Example 6. For example, a polypeptide, or combination of polypeptides, can protect a rodent such as a mouse against challenge with a Burkholderia spp. A polypeptide may have seroactive activity. As used herein, “seroactive activity” refers to the ability of a candidate polypeptide to react with antibody present in convalescent serum from an animal infected with a Burkholderia spp. such as, for example, B. thailandensis, B. mallei, B. pseudomallei, B. cenocepacia, or B. multivorans. In some aspects, the convalescent serum may be from an animal infected with B. thailandensis E264, B. pseudomallei K96243, B. mallei NCTC10229, B. cenocepacia AU1054, or B. multivorans ATCC17616.

A polypeptide may have immunoregulatory activity. As used herein, “immunoregulatory activity” refers to the ability of a polypeptide to act in a nonspecific manner to enhance an immune response to a particular antigen. Methods for determining whether a polypeptide has immunoregulatory activity are known in the art.

A polypeptide as described herein may have the characteristics of a polypeptide expressed by a reference microbe—i.e., a reference polypeptide. The characteristics can include, for example, molecular weight, mass fingerprint, amino acid sequence, or any combination thereof. The reference microbe can be a gram negative, preferably a member of the family Burkholderiaceae, preferably, Burkholderia spp. such as, for example, B. thailandensis, B. mallei, B. pseudomallei, B. cenocepacia, or B. multivorans. Exemplary strains of Burkholderia spp. and representative strains are listed in FIG. 5.

When the reference microbe is B. thailandensis E264, a candidate polypeptide can be considered to be a polypeptide as described herein if it has a molecular weight of 88 kDa, 84 kDa, 83 kDa, 81 kDa, 58 kDa, 56 kDa, 55 kDa, 44 kDa, 43 kDa, 42 kDa, 27 kDa, 24 kDa, or 19 kDa and has a mass fingerprint that is similar to the mass fingerprint of a metal-regulated polypeptide expressed by a reference microbe and having a molecular weight of—88 kDa, 84 kDa, 83 kDa, 81 kDa, 58 kDa, 56 kDa, 55 kDa, 44 kDa, 43 kDa, 42 kDa, 27 kDa, 24 kDa, or 19 kDa, respectively. Preferably, such polypeptides are metal-regulated. For instance, a candidate polypeptide can be a polypeptide as described herein if it has a molecular weight of 88 kDa and has a mass fingerprint similar to the mass fingerprint of an 88 kDa metal-regulated polypeptide produced by the reference strain B. thailandensis E264.

Alternatively, when the reference microbe is B. thailandensis E264, a candidate polypeptide can be considered to be a polypeptide as described herein if it has an amino acid sequence that is structurally similar, as described in detail below, to the amino acid sequence of SEQ ID NO:6, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:1, SEQ ID NO:7, SEQ ID NO:16, SEQ ID NO:22, SEQ ID NO:2, SEQ ID NO:13, SEQ ID NO:25, SEQ ID NO:21, SEQ ID NO:9, SEQ ID NO:11, or SEQ ID NO:10.

When the reference microbe is B. thailandensis E264, a candidate polypeptide can be considered to be a polypeptide as described herein if it has a molecular weight of 55 kDa, 40 kDa, 39 kDa, or 19 kDa and has a mass fingerprint that is similar to the mass fingerprint of a non-metal-regulated polypeptide expressed by a reference microbe and having a molecular weight of 55 kDa, 40 kDa, 39 kDa, or 19 kDa, respectively. Preferably, such polypeptides are non-metal-regulated. For instance, a candidate polypeptide can be a polypeptide as described herein if it has a molecular weight of 55 kDa and has a mass fingerprint similar to the mass fingerprint of a 55 kDa non-metal-regulated polypeptide produced by the reference strain B. thailandensis E264.

Alternatively, when the reference microbe is B. thailandensis E264, a candidate polypeptide can be considered to be a polypeptide as described herein if it has an amino acid sequence that is structurally similar, as described in detail below, to the amino acid sequence of SEQ ID NO:15, SEQ ID NO:12, SEQ ID NO:14, or SEQ ID NO:18.

When the reference microbe is B. thailandensis E264, a candidate polypeptide can be considered to be a polypeptide as described herein if it has a molecular weight of 85 kDa, 81 kDa, 78 kDa, 55 kDa, 36 kDa, or 8.5 kDa, respectively. Preferably, such polypeptides may or may not be metal-regulated. For instance, a candidate polypeptide can be a polypeptide as described herein if it has a molecular weight of 85 kDa and has a mass fingerprint similar to the mass fingerprint of an 85 kDa polypeptide produced by the reference strain B. thailandensis E264.

Alternatively, when the reference microbe is B. thailandensis E264, a candidate polypeptide can be considered to be a polypeptide as described herein if it has an amino acid sequence that is structurally similar, as described in detail below, to the amino acid sequence of SEQ ID NO:23, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:24, SEQ ID NO:17, SEQ ID NO:20, or SEQ ID NO:19.

As used herein, a polypeptide may be “structurally similar” to a reference polypeptide if the amino acid sequence of the polypeptide possesses a specified amount of sequence similarity and/or sequence identity compared to the reference polypeptide. A polypeptide also may be “structurally similar” to a reference polypeptide if the polypeptide exhibits a mass fingerprint possessing a specified amount of identity compared to a comparable mass fingerprint of the reference polypeptide. Thus, a polypeptide may be “structurally similar” to a reference polypeptide if, compared to the reference polypeptide, it possesses a sufficient level of amino acid sequence identity, amino acid sequence similarity, mass fingerprint similarity, or any combination thereof.

Polypeptide Sequence Similarity and Polypeptide Sequence Identity

Structural similarity of two polypeptides can be determined by aligning the residues of the two polypeptides (for example, a candidate polypeptide and any appropriate reference polypeptide described herein) to optimize the number of identical amino acids along the lengths of their sequences; gaps in either or both sequences are permitted in making the alignment in order to optimize the number of identical amino acids, although the amino acids in each sequence must nonetheless remain in their proper order. A reference polypeptide may be a polypeptide described herein or any known metal-regulated polypeptide, as appropriate. A candidate polypeptide is the polypeptide being compared to the reference polypeptide. A candidate polypeptide can be isolated, for example, from a microbe, or can be produced using recombinant techniques, or chemically or enzymatically synthesized.

Unless modified as otherwise described herein, a pair-wise comparison analysis of amino acid sequences can be carried out using the BESTFIT algorithm in the GCG package (version 10.2, Madison Wis.). Alternatively, polypeptides may be compared using the Blastp program of the BLAST 2 search algorithm, as described by Tatiana et al. (FEMS Microbiol Lett, 174:247-250 (1999)), and available on the National Center for Biotechnology Information (NCBI) website. The default values for all BLAST 2 search parameters may be used, including matrix=BLOSUM62; open gap penalty=11, extension gap penalty=1, gap x_dropoff=50, expect=10, wordsize=3, and filter on.

In the comparison of two amino acid sequences, structural similarity may be referred to by percent “identity” or may be referred to by percent “similarity.” “Identity” refers to the presence of identical amino acids. “Similarity” refers to the presence of not only identical amino acids but also the presence of conservative substitutions. A conservative substitution for an amino acid in a polypeptide may be selected from other members of the class to which the amino acid belongs. For example, it is well-known in the art of protein biochemistry that an amino acid belonging to a grouping of amino acids having a particular size or characteristic (such as charge, hydrophobicity, or hydrophilicity) can be substituted for another amino acid without altering the activity of a protein, particularly in regions of the protein that are not directly associated with biological activity. For example, nonpolar (hydrophobic) amino acids include alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, and tyrosine. Polar neutral amino acids include glycine, serine, threonine, cysteine, tyrosine, asparagine and glutamine. The positively charged (basic) amino acids include arginine, lysine and histidine. The negatively charged (acidic) amino acids include aspartic acid and glutamic acid. Conservative substitutions include, for example, Lys for Arg and vice versa to maintain a positive charge; Glu for Asp and vice versa to maintain a negative charge; Ser for Thr so that a free —OH is maintained; and Gln for Asn to maintain a free —NH2. Likewise, biologically active analogs of a polypeptide containing deletions or additions of one or more contiguous or noncontiguous amino acids that do not eliminate a functional activity—such as, for example, immunological activity—of the polypeptide are also contemplated.

Thus, as used herein, reference to a polypeptide as described herein and/or reference to the amino acid sequence of one or more SEQ ID NOs can include a polypeptide with at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% amino acid sequence similarity to the reference amino acid sequence.

Alternatively, as used herein, reference to a polypeptide as described herein and/or reference to the amino acid sequence of one or more SEQ ID NOs can include a polypeptide with at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% amino acid sequence identity to the reference amino acid sequence.

FIG. 6 shows cross-species sequence alignment for polypeptides having the amino acid sequences shown in SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:9. The alignment indicates amino acids that are conserved in the variants of each polypeptide across five Burkholderia species. The alignment also shows regions of variability in the variants of each polypeptide across five Burkholderia species. A person of ordinary skill in the art can deduce from such data regions of the polypeptide in which substitutions, particularly conservative substitutions, may be permitted without unduly affecting biological activity of the modified polypeptide.

Consequently, a polypeptide as described herein can include certain variants including, for example, homologous polypeptides that originate-biologically and/or recombinantly—from microbial species or strains other than the microbial species or strain from which the polypeptide was originally isolated and/or identified.

For example, a polypeptide as described herein can include a polypeptide commonly known as a TonB-dependent siderophore receptor. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:1. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:51, SEQ ID NO:101, SEQ ID NO:141, and SEQ ID NO:189.

For example, a polypeptide as described herein can include a polypeptide commonly known as resistance-nodulation-cell division (RND) superfamily efflux system outer membrane lipoprotein. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:2. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:52, SEQ ID NO:102, SEQ ID NO:142, and SEQ ID NO:190.

For example, a polypeptide as described herein can include a polypeptide commonly known as outer membrane ferric siderophore receptor. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:3. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:53, SEQ ID NO:143, and SEQ ID NO:191.

For example, a polypeptide as described herein can include a polypeptide commonly known as TonB-dependent heme/hemoglobin receptor family protein. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:4. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:54, SEQ ID NO:103, SEQ ID NO:144, and SEQ ID NO:192.

For example, a polypeptide as described herein can include a polypeptide commonly known as Fe(III) pyochelin receptor. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:5. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:55 and SEQ ID NO:145.

For example, a polypeptide as described herein can include a polypeptide commonly known as TonB-dependent siderophore receptor family protein. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:6. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:56, SEQ ID NO:104, and SEQ ID NO:146.

For example, a polypeptide as described herein can include a polypeptide commonly known as TonB-dependent copper receptor. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:7. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:57, SEQ ID NO:105, SEQ ID NO:147, and SEQ ID NO:193.

For example, a polypeptide as described herein can include a polypeptide commonly known as a TonB-dependent siderophore receptor. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:8. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:58, SEQ ID NO:106, SEQ ID NO:148, and SEQ ID NO:194.

For example, a polypeptide as described herein can include a polypeptide commonly known as an OmpA family protein. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:9. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:59, SEQ ID NO:107, SEQ ID NO:149, and SEQ ID NO:195.

For example, a polypeptide as described herein can include a polypeptide commonly known as OmpA family outer membrane protein. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:10. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:60, SEQ ID NO:108, SEQ ID NO:150, and SEQ ID NO:196.

For example, a polypeptide as described herein can include a polypeptide commonly known as OmpA family protein that differs from the OmpA family protein described above. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:11. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:61, SEQ ID NO:109, SEQ ID NO:151, and SEQ ID NO:197.

For example, a polypeptide as described herein can include a polypeptide commonly known as an outer membrane porin. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:12. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:62, SEQ ID NO:152, and SEQ ID NO:198.

For example, a polypeptide as described herein can include a polypeptide commonly known as an outer membrane porin that differs from the outer membrane porin described above. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:13. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:63, SEQ ID NO:110, SEQ ID NO:153, and SEQ ID NO:199.

For example, a polypeptide as described herein can include a polypeptide commonly known as an outer membrane porin that differs from the outer membrane porins described above. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:14. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:64, SEQ ID NO:111, SEQ ID NO:154, and SEQ ID NO:200.

For example, a polypeptide as described herein can include a polypeptide commonly known as an outer membrane porin that differs from the outer membrane porins described above. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:15. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:65, SEQ ID NO:112, SEQ ID NO:155, and SEQ ID NO:201.

For example, a polypeptide as described herein can include a polypeptide commonly known as a resistance-nodulation-cell division (RND) superfamily efflux system outer membrane lipoprotein. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:16. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:66, SEQ ID NO:156, and SEQ ID NO:202.

For example, a polypeptide as described herein can include a polypeptide commonly known as a resistance-nodulation-cell division (RND) superfamily efflux system outer membrane lipoprotein. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:17. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:67, SEQ ID NO:113, SEQ ID NO:157, and SEQ ID NO:203.

For example, a polypeptide as described herein can include a polypeptide commonly known as bacterioferritin (Bfr). One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:18. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:68, SEQ ID NO:114, SEQ ID NO:158, and SEQ ID NO:204.

For example, a polypeptide as described herein can include a polypeptide commonly known as bacterioferritin-associated ferredoxin (Bfd). One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:19. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:69, SEQ ID NO:115, SEQ ID NO:159, and SEQ ID NO:205.

For example, a polypeptide as described herein can include a polypeptide commonly known as dipeptide ABC transporter permease. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:20. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:70, SEQ ID NO:116, SEQ ID NO:160, and SEQ ID NO:206.

For example, a polypeptide as described herein can include a polypeptide commonly known as an outer membrane porin that differs from the outer membrane porins described above. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:21. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:71, SEQ ID NO:161, and SEQ ID NO:207.

For example, a polypeptide as described herein can include a polypeptide commonly known as a resistance-nodulation-cell division (RND) superfamily efflux system outer membrane lipoprotein. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:22. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:72, SEQ ID NO:117, and SEQ ID NO:208.

For example, a polypeptide as described herein can include a polypeptide commonly known as a TonB-dependent receptor. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:23. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:73, SEQ ID NO:118, SEQ ID NO:162, and SEQ ID NO:209.

For example, a polypeptide as described herein can include a polypeptide commonly known as a TonB-dependent receptor. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:24. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:74, SEQ ID NO:119, SEQ ID NO:163, and SEQ ID NO:210.

For example, a polypeptide as described herein can include a polypeptide commonly known as resistance-nodulation-cell division (RND) superfamily efflux transporter MFP subunit. One embodiment of this polypeptide is reflected in the amino acid sequence of SEQ ID NO:25. Variant embodiments are reflected in the amino acid sequences of SEQ ID NO:75, SEQ ID NO:120, SEQ ID NO:164, and SEQ ID NO:211.

Table 1 summarizes identifying characteristics of reference polypeptides natively expressed by reference microbe B. thailandensis E264.

TABLE 1 SEQ Molecular Present by Present by Iron- Immuno- Animal ID NO: GI Number Weight (kDa)^(b) 2D gel/MS^(c) iTRAQ regulation^(d) genicity^(e) protection^(f) 1 83718630 81 + + IR + + 2 83717404 55 + + IR + + 3 83716122 84 + + IR + + 4 83717803 83 ND + IR + + 5 83716062 81 ND + U + + 6 83720594 88 ND + IR + + 7 83717103 81 + + IR + + 8 83717123 81 ND ND U + ND 9 83720098 27 + + IR NT + 10 83719210 19 ND + IR NT + 11 83720431 24 + + IR NT + 12 83719322 40 + + NIR NT + 13 83716488 44 + + IR NT + 14 83718334 39 + + NIR NT + 15 83716254 55 + + NIR NT + 16 83720933 58 + + IR NT + 17 83718989 55 + + U NT + 18 83717287 19 ND + NIR NT + 19 83718020 8.5 ND ND U NT ND 20 83718178 36 ND + U NT + 21 83718226 42 + + IR NT + 22 83719074 56 + + IR NT + 23 83718695 85 ND ND U NT ND 24 83717289 78 ND ND U NT ND 25 83720836 43 + ND IR NT + ^(a)ID#, unique number assigned to each polypeptide for tracking purposes. ^(b)Molecular weight (predicted). ^(c)A protein is considered to be present (+) under iron restriction if detected by 2D gel/mass spectroscopy or in iTRAQ. ND, not detected. ^(d)Iron regulation was determined using criteria described in Example 5. IR, iron-regulated; NIR, non-iron regulated; U, iron regulation uncertain. ^(e)Immunogenicity of a single protein was determined by antibody and/or T cell recall responses to a purified recombinant protein in mice immunized with either the iron-restricted extract or a mix of recombinant proteins. NT, not tested. ^(f)protection is considered positive (+) if the protein was determined to be present in the immunizing extract for experiments shown in FIGS. 3 and 4, either by mass spectroscopy, in trial 3 by iTRAQ, or through production of antibodies to recombinant proteins from mice immunized with the protective extract vaccine.

A polypeptide as described herein also can be designed to provide one or more additional sequences such as, for example, the addition of coding sequences for added C-terminal and/or N-terminal amino acids that may facilitate purification by trapping on columns or use of antibodies. Such tags include, for example, histidine-rich tags that allow purification of polypeptides on nickel columns. Such gene modification techniques and suitable additional sequences are well known in the molecular biology arts.

A polypeptide as described herein also may be designed so that certain amino acids at the C-terminal and/or N-terminal are deleted. For example, one difference between the amino acid sequences of SEQ ID NO:1 and SEQ ID NO:51 is that SEQ ID NO:51 possesses an N-terminal 17 amino acid addition that is not present in the reference polypeptide amino acid sequence of SEQ ID NO:1. Similar exemplary N-terminal additions, typically varying from about five amino acids to about 50 amino acids, are apparent when one compares, for example, the reference amino acid sequence of SEQ ID NO:101, SEQ ID NO:141, SEQ ID NO:189, SEQ ID NO:190, SEQ ID NO:59, or SEQ ID NO:107 with certain variant embodiments of the respective amino acid sequence. Other amino acids additions and/or deletions, at either the N-terminal or the C-terminal, are possible.

A “modification” of a polypeptide as described herein includes a polypeptide (or an analog thereof such as, e.g., a fragment thereof) that is chemically or enzymatically derivatized at one or more constituent amino acids. Such a modification can include, for example, a side chain modification, a backbone modification, an N-terminal modification, and/or a C-terminal modification such as, for example, acetylation, hydroxylation, methylation, amidation, and the attachment of a carbohydrate and/or lipid moiety, a cofactor, and the like, and combinations thereof. Modified polypeptides as described herein may retain the biological activity—such as, for example, immunological activity—of the unmodified polypeptide or may exhibit a reduced or increased biological activity compared to the unmodified polypeptide.

A polypeptide as described herein (including a biologically active analog thereof and/or a modification thereof) can include a native (naturally occurring), a recombinant, a chemically synthesized, or an enzymatically synthesized polypeptide. For example, a polypeptide as described herein may be prepared by isolating the polypeptide from a natural source or may be prepared recombinantly by conventional methods including, for example, preparation as fusion proteins in bacteria or other host cells.

A polypeptide expressed by a reference microbe can be obtained by growing the reference microbe under low metal conditions as described herein and the subsequent isolation of a polypeptide by the processes disclosed herein. Alternatively, a polypeptide expressed by a reference microbe can be obtained by identifying coding regions expressed at higher levels when the microbe is grown in low metal conditions—i.e., metal-regulated. A metal-regulated coding region can be cloned and expressed, and the expressed metal-regulated polypeptide may be identified by the processes described herein. A candidate polypeptide can be isolatable from a microbe or identified from a microbe, preferably a gram negative microbe, more preferably, a member of the family Burkholderiaceae, preferably, Burkholderia spp. such as, for example, B. thailandensis, B. mallei, B. pseudomallei, B. cenocepacia, or B. multivorans. A candidate polypeptide may also be produced using enzymatic or chemical techniques.

Mass Fingerprint Similarity

A candidate polypeptide may be evaluated by mass spectrometric analysis to determine whether the candidate polypeptide has a mass fingerprint similar to one of the polypeptides expressed by a reference microbe and referred to above by molecular weight. Typically, the candidate polypeptide can be isolated, for instance by resolving the candidate polypeptide by gel electrophoresis and excising the portion of the gel containing the candidate polypeptide. Any gel electrophoresis method that separates polypeptides based on differing characteristics can be used, including one-dimensional or two-dimensional gel electrophoresis, as well as liquid chromatographic separation based on, for instance, hydrophobicity, pI, or size. The candidate polypeptide can be fragmented, for instance by digestion with a protease. Preferably, the protease can cleave the peptide bond on the carboxy-terminal side of the amino acid lysine and the amino acid arginine, except when the amino acid following the lysine or the arginine is a proline. An example of such a protease is trypsin. Methods for digesting a polypeptide with trypsin are routine and known in the art. An example of such a method is disclosed in Example 3.

Methods for the mass spectrometric analysis of polypeptides are routine and known in the art and include, but are not limited to, nano high-pressure liquid chromatography electrospray tandem mass spectrometry (nanoLC-EDI-MS/MS). Often, when a candidate polypeptide is analyzed by mass spectroscopy, both the candidate polypeptide and the reference polypeptide—i.e., the polypeptide from the reference microbe—are prepared and analyzed together, thereby decreasing any potential artifacts resulting from differences in sample handling and running conditions. Preferably, all reagents used to prepare and analyze the two polypeptides are the same. For instance, the polypeptide from the reference microbe and the candidate polypeptide are isolated under substantially the same conditions, fragmented under substantially the same conditions, and analyzed by nanoLC-EDI-MS/MS on the same machine under substantially the same conditions. A candidate polypeptide may be considered to be “structurally similar” to a reference polypeptide if it exhibits a mass fingerprint possessing at least 80%, at least 90%, at least 95%, or substantially all of the m/z values present in the spectrum of the reference microbe polypeptide and above the background level of noise are also present in the spectrum of the candidate polypeptide. (See, e.g., United States Patent Application Publication No. 2006/0233824 A1).

In another aspect, a polypeptide can be considered to be a polypeptide as described herein if it has a molecular weight of a reference polypeptide described herein and has a mass fingerprint that includes a subpopulation including at least a specified percentage of the polypeptide fragments of the mass fingerprint of the reference polypeptide.

The mass fingerprint of a candidate polypeptide can be determined by a mass spectrometric method, for instance by nanoLC-EDI-MS/MS. The mass fingerprint of a candidate polypeptide will generally have additional polypeptide fragments and, therefore, can have additional m/z values other than those identified in any particular analysis. When the candidate polypeptide is being compared to a polypeptide as described herein, the candidate polypeptide can be isolatable from a microbe, preferably a gram negative microbe, more preferably, a member of the family Burkholderiaceae, preferably, Burkholderia spp. such as, for example, B. thailandensis, B. mallei, B. pseudomallei, B. cenocepacia, or B. multivorans.

Metal-regulated candidate polypeptides can be obtained by growing a microbe under low metal conditions and subsequently isolating a polypeptide by the processes described herein. Non-metal-regulated candidate polypeptides can be obtained by growing a microbe under low metal conditions or high metal conditions and subsequently isolating a polypeptide by the processes described herein. Alternatively, a candidate polypeptide can be obtained by recombinant expression of a polynucleotide that encodes the candidate polypeptide.

Polynucleotide Sequence Similarity and Polynucleotide Sequence Identity

Polypeptides as described herein also may be identified in terms of the polynucleotide that encodes the polypeptide. Thus, this disclosure provides polynucleotides that encode a polypeptide as described herein or hybridize, under standard hybridization conditions, to a polynucleotide that encodes a polypeptide as described herein, and the complements of such polynucleotide sequences.

As used herein, reference to a polynucleotide as described herein and/or reference to the nucleic acid sequence of one or more SEQ ID NOs can include polynucleotides having a sequence identity of at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to an identified reference polynucleotide sequence.

In this context, “sequence identity” refers to the identity between two polynucleotide sequences. Sequence identity is generally determined by aligning the bases of the two polynucleotides (for example, aligning the nucleotide sequence of the candidate sequence and a nucleotide sequence that includes, for example, the nucleotide sequence of SEQ ID NO:26 or SEQ ID NO:27) to optimize the number of identical nucleotides along the lengths of their sequences; gaps in either or both sequences are permitted in making the alignment in order to optimize the number of shared nucleotides, although the nucleotides in each sequence must nonetheless remain in their proper order. A candidate sequence is the sequence being compared to a known sequence—e.g., a nucleotide sequence that includes the nucleotide sequence of, for example, SEQ ID NO:76 or SEQ ID NO:77. For example, two polynucleotide sequences can be compared using the Blastn program of the BLAST 2 search algorithm, as described by Tatiana et al., (FEMS Microbiol Lett., 174:247-250 (1999)), and available on the world wide web at ncbi.nlm.nih.gov/BLAST/. The default values for all BLAST 2 search parameters may be used, including reward for match=1, penalty for mismatch=−2, open gap penalty=5, extension gap penalty=2, gap x_dropoff=50, expect=10, wordsize=11, and filter on.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as a TonB-dependent siderophore receptor. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:26. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:76, SEQ ID NO:121, SEQ ID NO:165, and SEQ ID NO:212.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as resistance-nodulation-cell division (RND) superfamily efflux system outer membrane lipoprotein. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:27. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:77, SEQ ID NO:122, SEQ ID NO:166, and SEQ ID NO:213.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as outer membrane ferric siderophore receptor. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:28. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:78, SEQ ID NO:167, and SEQ ID NO:214.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as TonB-dependent heme/hemoglobin receptor family protein. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:29. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:79, SEQ ID NO:123, SEQ ID NO:168, and SEQ ID NO:215.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as Fe(III) pyochelin receptor. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:30. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:80 and SEQ ID NO:169.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as TonB-dependent siderophore receptor family protein. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:31. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:81, SEQ ID NO:124, and SEQ ID NO:170.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as TonB-dependent copper receptor. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:32. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:82, SEQ ID NO:125, SEQ ID NO:171, and SEQ ID NO:216.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as a TonB-dependent siderophore receptor. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:33. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:83, SEQ ID NO:126, SEQ ID NO:172, and SEQ ID NO:217.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as an OmpA family protein. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:34. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:84, SEQ ID NO:127, SEQ ID NO:173, and SEQ ID NO:218.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as OmpA family outer membrane protein. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:35. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:85, SEQ ID NO:128, SEQ ID NO:174, and SEQ ID NO:219.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as OmpA family protein that differs from the OmpA family protein described above. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:36. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:86, SEQ ID NO:129, SEQ ID NO:175, and SEQ ID NO:220.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as an outer membrane porin. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:37. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:87, SEQ ID NO:176, and SEQ ID NO:221.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as an outer membrane porin that differs from the outer membrane porin described above. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:38. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:88, SEQ ID NO:130, SEQ ID NO:177, and SEQ ID NO:222.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as an outer membrane porin that differs from the outer membrane porins described above. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:39. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:89, SEQ ID NO:131, SEQ ID NO:178, and SEQ ID NO:223.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as an outer membrane porin that differs from the outer membrane porins described above. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:40. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:90, SEQ ID NO:132, SEQ ID NO:179, and SEQ ID NO:224.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as a resistance-nodulation-cell division (RND) superfamily efflux system outer membrane lipoprotein. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:41. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:91, SEQ ID NO:180, and SEQ ID NO:225.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as a resistance-nodulation-cell division (RND) superfamily efflux system outer membrane lipoprotein. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:42. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:92, SEQ ID NO:133, SEQ ID NO:181, and SEQ ID NO:226.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as bacterioferritin (Bfr). One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:43. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:93, SEQ ID NO:134, SEQ ID NO:182, and SEQ ID NO:227.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as bacterioferritin-associated ferredoxin (Bfd). One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:44. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:94, SEQ ID NO:135, SEQ ID NO:183, and SEQ ID NO:228.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as dipeptide ABC transporter permease. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:45. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:95, SEQ ID NO:136, SEQ ID NO:184, and SEQ ID NO:229.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as an outer membrane porin that differs from the outer membrane porins described above. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:46. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:96, SEQ ID NO:185, and SEQ ID NO:230.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as a resistance-nodulation-cell division (RND) superfamily efflux system outer membrane lipoprotein. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:47. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:97, SEQ ID NO:137, and SEQ ID NO:231.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as a TonB-dependent receptor. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:48. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:98, SEQ ID NO:138, SEQ ID NO:186, and SEQ ID NO:232.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as a TonB-dependent receptor. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:49. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:99, SEQ ID NO:139, SEQ ID NO:187, and SEQ ID NO:233.

For example, a polynucleotide as described herein can include a polynucleotide that encodes a polypeptide commonly known as resistance-nodulation-cell division (RND) superfamily efflux transporter MFP subunit. One embodiment of this polynucleotide is reflected in the polynucleotide sequence of SEQ ID NO:50. Variant embodiments are reflected in the polynucleotide sequences of SEQ ID NO:100, SEQ ID NO:140, SEQ ID NO:188, and SEQ ID NO:234.

Finally, a polynucleotide as described herein can include any polynucleotide that encodes a polypeptide as described herein. Thus, the nucleotide sequence of the polynucleotide may be deduced from the amino acid sequence that is to be encoded by the polynucleotide.

This disclosure also provides whole cell preparations of a microbe, where the microbe expresses one or more of the polypeptides described herein. The cells present in a whole cell preparation may be inactivated such that the cells cannot replicate but the immunological activity of the polypeptides as described herein expressed by the microbe is maintained. Typically, the cells may be killed by exposure to agents such as glutaraldehyde, formalin, or formaldehyde.

Compositions

A composition as described herein may include at least one isolated polypeptide described herein, or a number of polypeptides that is an integer greater than one (e.g., at least two, at least three, at least four). Unless a specific level of sequence similarity and/or identity is expressly indicated herein (e.g., at least 80% sequence similarity, at least 90% sequence identity, etc.), reference to the amino acid sequence of an identified SEQ ID NO includes variants having the levels of sequence similarity and/or the levels of sequence identity described herein in the section headed “Polypeptide sequence similarity and polypeptide sequence identity.

A recombinantly-produced polypeptide may be expressed from a vector that permits expression of the polypeptide when the vector is introduced into an appropriate host cell. A host cell may be constructed to produce one or more recombinantly-produced polypeptides as described herein and, therefore, can include one more vectors that include at least one polynucleotide that encodes a polypeptide as described herein. Thus, each vector can include one or more polynucleotides as described herein—i.e., a polynucleotide that encodes a polypeptide as described herein.

Certain compositions such as, for example, those including recombinantly-produced polypeptides, can include a maximum number of polypeptides. In some embodiments, the maximum number of polypeptides can refer to the maximum total number of polypeptides. Certain compositions can include, for example, no more than 50 polypeptides such as, for example, no more than 40 polypeptides, no more than 30 polypeptides, no more than 25 polypeptides, no more than 20 polypeptides, no more than 15 polypeptides, no more than 10 polypeptides, no more than eight polypeptides, no more than seven polypeptides, no more than six polypeptides, no more than five polypeptides, no more than four polypeptides, no more than three polypeptides, no more than two polypeptides, or no more than one polypeptide. In other embodiments, a maximum number of recombinantly-produced polypeptides may be specified in a similar manner. In still other embodiments, a maximum number of nonrecombinantly-produced polypeptides may be specified in a similar manner.

A composition can include polypeptides isolatable from one microbe, or can be isolatable from a combination of two or more microbes. For instance, a composition can include polypeptides isolatable from two or more Burkholderia spp., or from a Burkholderia spp. and a different microbe that is not a member of the genus Burkholderia. In certain embodiments, a composition can include a whole cell preparation in which the whole cell expresses one or more of the polypeptides as described herein. In some of these embodiments, the whole cell can be a Burkholderia spp. In some embodiments, a composition can include whole cell preparations from two, three, four, five, or six strains.

Optionally, a polypeptide as described herein can be covalently bound or conjugated to a carrier polypeptide to improve the immunological properties of the polypeptide. Useful carrier polypeptides are known in the art. The chemical coupling of polypeptides as described herein can be carried out using known and routine methods. For instance, various homobifunctional and/or heterobifunctional cross-linker reagents such as bis(sulfosuccinimidyl) suberate, bis(diazobenzidine), dimethyl adipimidate, dimethyl pimelimidate, dimethyl superimidate, disuccinimidyl suberate, glutaraldehyde, m-maleimidobenzoyl-N-hydroxysuccinimide, sulfo-m-maleimidobenzoyl-N-hydroxysuccinimide, sulfosuccinimidyl 4-(N-maleimidomethyl) cycloheane-1-carboxylate, sulfosuccinimidyl 4-(p-maleimido-phenyl) butyrate and (1-ethyl-3-(dimethyl-aminopropyl) carbodiimide can be used (see, for instance, Harlow and Lane, Antibodies, A Laboratory Manual, generally and Chapter 5, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, N.Y. (1988)).

The compositions as described herein optionally further include a pharmaceutically acceptable carrier. “Pharmaceutically acceptable” refers to a diluent, carrier, excipient, salt, etc., that is compatible with the other ingredients of the composition, and not deleterious to the recipient thereof. Typically, the composition includes a pharmaceutically acceptable carrier when the composition is used as described herein. Exemplary pharmaceutically acceptable carriers include buffer solutions and generally exclude blood products such as, for example, whole blood and/or plasma. The compositions as described herein may be formulated in pharmaceutical preparations in a variety of forms adapted to the chosen route of administration, including routes suitable for stimulating an immune response to an antigen. Thus, a composition as described herein can be administered via known routes including, for example, oral; parenteral including intradermal, transcutaneous and subcutaneous; intramuscular, intravenous, intraperitoneal, etc. and topically, such as, intranasal, intrapulmonary, intramammary, intravaginal, intrauterine, intradermal, transcutaneous and rectally, etc. It is foreseen that a composition can be administered to a mucosal surface, such as by administration to the nasal or respiratory mucosa (e.g., via a spray or aerosol), in order to stimulate mucosal immunity, such as production of secretory IgA antibodies, throughout the animal's body.

A composition as described herein can also be administered via a sustained or delayed release implant. Implants suitable for use according to the invention are known and include, for example, those disclosed in International Publication No. WO 2001/037810 and/or International Publication No. WO 1996/001620. Implants can be produced at sizes small enough to be administered by aerosol or spray. Implants also can include nanospheres and microspheres.

A composition as described herein may be administered in an amount sufficient to treat certain conditions as described herein. The amount of polypeptides or whole cells present in a composition as described herein can vary. For instance, the dosage of polypeptides can be between 0.01 micrograms (μg) and 300 mg, typically between 0.1 mg and 10 mg. When the composition is a whole cell preparation, the cells can be present at a concentration of, for instance, 10² bacteria/ml, 10³ bacteria/ml, 10⁴ bacteria/ml, 10⁵ bacteria/ml, 10⁶ bacteria/ml, 10⁷ bacteria/ml, 10⁸ bacteria/ml, or 10⁹ bacteria/ml. For an injectable composition (e.g. subcutaneous, intramuscular, etc.) the polypeptides may be present in the composition in an amount such that the total volume of the composition administered is 0.5 ml to 5.0 ml, typically 1.0 to 2.0 ml. When the composition is a whole cell preparation, the cells are preferably present in the composition in an amount that the total volume of the composition administered is 0.5 ml to 5.0 ml, typically 1.0 to 2.0 ml. The amount administered may vary depending on various factors including, but not limited to, the specific polypeptides chosen, the weight, physical condition and age of the animal, and the route of administration. Thus, the absolute weight of the polypeptide included in a given unit dosage form can vary widely, and depends upon factors such as the species, age, weight and physical condition of the animal, as well as the method of administration. Such factors can be determined by one of skill in the art. Other examples of dosages suitable for the invention are disclosed in U.S. Pat. No. 6,027,736.

The formulations may be conveniently presented in unit dosage form and may be prepared by methods well known in the art of pharmacy. Methods of preparing a composition with a pharmaceutically acceptable carrier include the step of bringing the active compound (e.g., a polypeptide or whole cell as described herein) into association with a carrier that constitutes one or more accessory ingredients. In general, the formulations are prepared by uniformly and intimately bringing the active compound into association with a liquid carrier, a finely divided solid carrier, or both, and then, if necessary, shaping the product into the desired formulations.

A composition including a pharmaceutically acceptable carrier also can include an adjuvant. An “adjuvant” refers to an agent that can act in a nonspecific manner to enhance an immune response to a particular antigen, thus potentially reducing the quantity of antigen necessary in any given immunizing composition, and/or the frequency of injection necessary in order to generate an adequate immune response to the antigen of interest. Adjuvants may include, for example, IL-1, IL-2, emulsifiers, muramyl dipeptides, dimethyl dioctadecyl ammonium bromide (DDA), avridine, aluminum hydroxide, oils, saponins, alpha-tocopherol, polysaccharides, emulsified paraffins (including, for instance, those available from under the tradename EMULSIGEN from MVP Laboratories, Ralston, Nebr.), ISA-70, RIBI and other substances known in the art. It is expected that polypeptides as described herein will have immunoregulatory activity and that such polypeptides may be used as adjuvants that directly act as T cell and/or B cell activators or act on specific cell types that enhance the synthesis of various cytokines or activate intracellular signaling pathways. Such polypeptides are expected to augment the immune response to increase the protective index of the existing composition.

In another embodiment, a composition as described herein including a pharmaceutically acceptable carrier can include a biological response modifier, such as, for example, IL-2, IL-4 and/or IL-6, TNF, IFN-α, IFN-γ, and other cytokines that effect immune cells. An immunizing composition can also include other components known in the art such as an antibiotic, a preservative, an anti-oxidant, or a chelating agent.

Methods of Making

This disclosure also provides methods for obtaining the polypeptides described herein. The polypeptides and whole cells as described herein may be isolatable from a member of the family Burkholderiaceae, preferably, Burkholderia spp. such as, for example, B. thailandensis, B. mallei, B. pseudomallei, B. cenocepacia, or B. multivorans. Other gram negative microbes from which polypeptides can be isolated include, for example, Achromobacter spp., Ralstonia spp., Pseudomonas spp., Bordetella spp., and Acinetobacter spp. Microbes useful for obtaining polypeptides as described herein and making whole cell preparations are commercially available from a depository such as American Type Culture Collection (ATCC). In addition, such microbes are readily obtainable by techniques routine and known to the art. The microbes may be derived from an infected animal as a field isolate, and used to obtain the polypeptides and/or the whole cell preparations as described herein, or stored for future use, for example, in a frozen repository at from −20° C. to −95° C., or from −40° C. to −50° C., in bacteriological media containing 20% glycerol, and other like media.

When a polypeptide as described herein is to be obtained from a microbe, the microbe can be incubated under low metal conditions. As used herein, the phrase “low metal conditions” refers to an environment, typically bacteriological media, that contains an amount of a free metal that causes a microbe to express metal-regulated polypeptides at a detectable level. As used herein, the phrase “high metal conditions” refers to an environment that contains an amount of a free metal that causes a microbe to express a metal-regulated polypeptide at a decreased level compared to expression of the metal-regulated polypeptide under low metal conditions. In some cases, “high metal conditions” can refer to an environment that causes a cell to fail to express one or more of the metal-regulated polypeptides described herein at a detectable level.

In some cases, “high metal conditions” can include a metal-rich natural environment and/or culture in a metal-rich medium without a metal chelator. In contrast, in some cases, “low metal conditions” can include culture in a medium that includes a metal chelator, as described in more detail below. Metals are those present in the periodic table under Groups 1 through 17 (IUPAC notation; also referred to as Groups I-A, II-A, III-B, IV-B, V-B, VI-B, VII-B, VIII, I-B, II-B, III-A, IV-A, V-A, VI-A, and VII-A, respectively, under CAS notation). Preferably, metals are those in Groups 2 through 12, more preferably, Groups 3-12. Even more preferably, the metal is iron, zinc, copper, magnesium, nickel, cobalt, manganese, molybdenum, or selenium, most preferably, iron.

Low metal conditions are generally the result of the addition of a metal chelating compound to a bacteriological medium, the use of a bacteriological medium that contains low amounts of a metal, or the combination thereof. High metal conditions are generally present when a chelator is not present in the medium, when a metal is added to the medium, or the combination thereof. Examples of metal chelators include natural and synthetic compounds. Examples of natural compounds include plant phenolic compounds, such as flavenoids. Examples of flavenoids include the copper chelators catechin and naringenin, and the iron chelators myricetin and quercetin. Examples of synthetic copper chelators include, for instance, tetrathiomolybdate, and examples of synthetic zinc chelators include, for instance, N,N,N′,N′-Tetrakis (2-pyridylmethyl)-ethylene diamine Examples of synthetic iron chelators include 2,2′-dipyridyl (also referred to in the art as θ,θ′-bipyridyl), 8-hydroxyquinoline, ethylenediamine-di-O-hydroxyphenylacetic acid (EDDHA), desferrioxamine methanesulphonate (desferol), transferrin, lactoferrin, ovotransferrin, biological siderophores, such as, the catecholates and hydroxamates, and citrate. An example of a general divalent cation chelator is CHELEX resin. Preferably, 2,2′-dipyridyl is used for the chelation of iron. Typically, 2,2′-dipyridyl is added to the media at a concentration of at least 300 micrograms/milliliter (μg/ml), at least 600 μg/ml, or at least 900 μg/ml. High levels of 2,2′-dipyridyl can be, for example, 1200 μg/ml, 1500 μg/ml, or 1800 μg/ml.

The B. thailandensis genome encodes a ferric uptake regulator (Fur) homolog. The Fur protein controls the uptake of metal ions by regulating the expression of iron receptors such as the siderophore receptor proteins in response to iron limitation. It also plays a role in oxidative stress resistance and virulence. It is expected that a gram negative organism, preferably, a Burkholderia spp., with a mutation in the fur coding region will result in the constitutive expression of many, if not all, of the metal-regulated polypeptides as described herein. The production of a fur mutation in a gram negative, preferably, a Burkholderia spp., can be produced using routine methods including, for instance, transposon, chemical, or site-directed mutagenesis useful for generating gene missense or knock-out mutations in gram negative bacteria.

The medium used to incubate the microbe and the volume of media used to incubate the microbe can vary. When a microbe is being evaluated for the ability to produce one or more of the polypeptides described herein, the microbe can be grown in a suitable volume, for instance, 10 milliliters to 1 liter of medium. When a microbe is being grown to obtain polypeptides for use in, for instance, administration to animals, the microbe may be grown in a fermenter to allow the isolation of larger amounts of polypeptides. Methods for growing microbes in a fermenter are routine and known to the art. The conditions used for growing a microbe preferably include a metal chelator, more preferably an iron chelator, for instance 2,2′-dipyridyl, a pH of between 6.5 and 7.5, preferably between 6.9 and 7.1, and a temperature of 37° C.

In some aspects of the invention, a microbe may be harvested after growth. Harvesting includes concentrating the microbe into a smaller volume and suspending in a media different than the growth media. Methods for concentrating a microbe are routine and known in the art, and include, for example, filtration or centrifugation. Typically, the concentrated microbe is suspended in an appropriate buffer. An example of a buffer that can be used contains Tris-base (7.3 grams/liter), at a pH of 8.5. Optionally, the final buffer also minimizes proteolytic degradation. This can be accomplished by having the final buffer at a pH of greater than 8.0, preferably, at least 8.5, and/or including one or more proteinase inhibitors (e.g., phenylmethanesulfonyl fluoride). Optionally, the concentrated microbe is frozen at −20° C. or below until disrupted.

When the microbe is to be used as a whole cell preparation, the harvested cells may be processed using routine and known methods to inactivate the cells. Alternatively, when a microbe is to be used to prepare polypeptides as described herein, the microbe may be disrupted using chemical, physical, or mechanical methods routine and known to the art, including, for example, boiling, French press, sonication, digestion of peptidoglycan (for instance, by digestion with lysozyme), or homogenization. An example of a suitable device useful for homogenization is a model C500-B AVESTIN homogenizer, (Avestin Inc., Ottawa, Canada). As used herein, “disruption” refers to the breaking up of the cell. Disruption of a microbe can be measured by methods that are routine and known to the art, including, for instance, changes in optical density. Typically, a microbe is subjected to disruption until the percent transmittance is increased by 20% when a 1:100 dilution is measured. When physical or mechanical methods are used, the temperature during disruption is typically kept low, preferably at 4° C., to further minimize proteolytic degradation. When chemical methods are used the temperature may be increased to optimize for the cell disruption. A combination of chemical, physical, and mechanical methods may also be used to solubilize the cell wall of microbe. As used herein, the term “solubilize” refers to dissolving cellular materials (e.g., polypeptides, nucleic acids, carbohydrates) into the aqueous phase of the buffer in which the microbe was disrupted, and the formation of aggregates of insoluble cellular materials. Without intending to be limited by theory, the conditions for solubilization are believed to result in the aggregation of polypeptides as described herein into insoluble aggregates that are large enough to allow easy isolation by, for instance, centrifugation.

The insoluble aggregates that include one or more of the polypeptides as described herein may be isolated by methods that are routine and known to the art. Preferably, the insoluble aggregates are isolated by centrifugation. Typically, centrifugation of polypeptides, such as membrane polypeptides, can be accomplished by centrifugal forces of 100,000×g. The use of such centrifugal forces requires the use of ultracentrifuges, and scale-up to process large volumes of sample is often difficult and not economical with these types of centrifuges. The methods described herein provide for the production of insoluble aggregates large enough to allow the use of continuous flow centrifuges, for instance T-1 Sharples (Alfa Laval, Inc., Richmond, Va.), which can be used with a flow rate of 250 ml/minute at 17 psi at a centrifugal force of 46,000×g to 60,000×g. Other large scale centrifuges can be used, such as the tubular bowl, chamber, and disc configurations. Such centrifuges are routinely used and known in the art, and are commercially available from such manufactures as Pennwalt, Ltd., GEA Westfalia Separator Division (GEA Mechanical Equipment US, Inc.), or Alpha Laval, Inc.

The final harvested proteins can be washed and/or dialyzed against an appropriate buffer using conventional methods such as, for example, diafiltration, precipitation, hydrophobic chromatography, ion-exchange chromatography, affinity chromatography, or ultra-filtration, followed by washing the polypeptides, for instance, in alcohol, by diafiltration. After isolation, the polypeptides suspended in buffer and stored at low temperature, for instance, −20° C. or below.

In those aspects as described herein where a whole cell preparation is to be made, after growth a microbe can be killed with the addition of an agent such as glutaraldehyde, formalin, or formaldehyde, at a concentration sufficient to inactivate the cells in the culture. For instance, formalin can be added at a concentration of 0.3% (vol:vol). After a period of time sufficient to inactivate the cells, the cells can be harvested by, for instance, diafiltration and/or centrifugation, and washed.

In other aspects, an isolated polypeptide as described herein may be prepared recombinantly. When prepared recombinantly, a polynucleotide encoding the polypeptide may be identified and cloned into an appropriate expression host as described below in Example 6. The recombinant expression host may be grown in an appropriate medium, disrupted, and the polypeptides isolated as described above.

Methods of Use

In another aspect, this disclosure further provides methods of using the compositions as described herein. The methods include administering to an animal an effective amount of a composition as described herein. The animal can be, for instance, avian (including, for instance, chickens or turkeys), bovine (including, for instance, cattle), caprine (including, for instance, goats), ovine (including, for instance, sheep), porcine (including, for instance, swine), bison (including, for instance, buffalo), equine (including, for instance, horses), a companion animal (including, for instance, dogs or cats), members of the family Cervidae (including, for instance, deer, elk, moose, caribou and reindeer), or human.

In some aspects, the methods may further include additional administrations (e.g., one or more booster administrations) of the composition to the animal to enhance or stimulate a secondary immune response. A booster can be administered at a time after the first administration, for instance, one to eight weeks, preferably two to four weeks, after the first administration of the composition. Subsequent boosters can be administered one, two, three, four, or more times annually. Without intending to be limited by theory, it is expected that in some aspects as described herein annual boosters will not be necessary, as an animal will be challenged in the field by exposure to microbes expressing polypeptides present in the compositions having epitopes that are identical to or structurally related to epitopes present on polypeptides of the composition administered to the animal.

In one embodiment, the method can involve making antibody, for instance by inducing the production of antibody in an animal or by recombinant techniques. As used herein, the term “antibody”—when not preceded by a definite or indefinite article—can be used generically to refer to any preparation that includes at least one molecular species of immunoglobulin or a fragment (e.g., scFv, Fab, F(ab′)₂ or Fv or other modified fragment) thereof. Therefore, “antibody” can generically include one or more monoclonal antibodies and/or a polyclonal antibody preparation. Antibody produced by the method can include antibody that specifically binds at least one polypeptide present in the composition. In this context, an “effective amount” is an amount effective to result in the production of antibody in the animal. Methods for determining whether an animal has produced antibody that specifically binds a polypeptide present in a composition can be determined as described herein. This disclosure therefore further provides antibody that specifically binds to a polypeptide as described herein, and compositions including such antibody.

The method may be used to produce antibody that specifically binds to a polypeptide expressed by a microbe other than the microbe from which the polypeptide of the composition were isolated. As used herein, antibody that can “specifically bind” a polypeptide is antibody that interacts with the epitope of the antigen that induced the synthesis of the antibody, or interacts with a structurally related epitope. At least some of the polypeptides present in the compositions as described herein typically include epitopes that are conserved in the polypeptides of different species and different genera of microbes. Accordingly, antibody produced using a composition derived from one microbe is expected to bind to polypeptides expressed by other microbes and provide broad spectrum protection against gram negative organisms. Examples of gram negative microbes to which the antibody may specifically bind are Burkholderiaceae, preferably, Burkholderia spp. such as, for example, B. thailandensis, B. mallei, B. pseudomallei, B. cenocepacia, or B. multivorans. Therefore, antibody produced using a composition of polypeptides as described herein may be used to identify and characterize polypeptides as described herein independent of the origin, source, and/or manner of obtaining the polypeptide.

In another aspect, this disclosure provides the use of such antibody to target a microbe expressing a polypeptide as described herein or a polypeptide having an epitope structurally related to an epitope present on a polypeptide as described herein. A compound can be covalently bound to an antibody, where the compound can be, for instance, a toxin. Likewise, such compounds can be covalently bound to a bacterial siderophore to target the microbe. The chemical coupling or conjugation of an antibody as described herein, or a portion thereof (such as a Fab fragment), can be carried out using known and routine methods.

In another aspect, this disclosure provides methods for treating an infection in an animal, including a human, caused by a gram negative microbe, preferably by a member of the family Burkholderiaceae, preferably, Burkholderia spp. such as, for example, B. thailandensis, B. mallei, B. pseudomallei, B. cenocepacia, or B. multivorans. As used herein, the term “infection” refers to the presence of a gram negative microbe in an animal's body, which may or may not be clinically apparent. An animal with an infection by a member of the genus Burkholderia that is not clinically apparent is often referred to as an asymptomatic carrier.

Treating an infection can be prophylactic or, alternatively, therapeutic—in this context, treatment after a subject manifests one or more indication of infection by a microbe. Generally, treatment that is prophylactic—in this context, initiated before a subject is infected by a microbe or while an infection remains subclinical—is referred to herein as treatment of a subject that is “at risk” of infection. As used herein, the term “at risk” refers to an animal that may or may not actually possess the described risk—in this context, an animal that may or may not be infected by a particular microbe. Thus, typically, an animal “at risk” of infection by a microbe is an animal present in an area where animals have been identified as infected by the microbe and/or is likely to be exposed to the microbe even if the animal has not yet manifested any detectable indication of infection by the microbe and regardless of whether the animal may harbor a subclinical amount of the microbe. Accordingly, administration of a composition can be performed before, during, or after the animal has first contact with the microbe. Treatment initiated after the animal's first contact with the microbe may result in decreasing the severity of symptoms and/or clinical signs of infection by the microbe, completely removing the microbe, and/or decreasing the likelihood of experiencing a clinically evident infection compared to an animal to which the composition is not administered. The method includes administering an effective amount of the composition as described herein to an animal having, or at risk of having, an infection caused by a gram negative microbe, and determining whether the number of microbes causing the infection has decreased. In this context, an “effective amount” is an amount effective to reduce the number of the specified microbes in an animal or reduce the likelihood that the animal experiences a clinically-evident infection compared to an animal to which the composition is not administered. Methods for determining whether an infection is caused by a gram negative microbe are routine and known in the art, as are methods for determining whether the infection has decreased.

In another aspect, the present invention is directed to methods for treating one or more symptoms or clinical signs of certain conditions in an animal that may be caused by infection by a gram negative microbe, preferably by a member of the family Burkholderiaceae, preferably, Burkholderia spp. such as, for example, B. thailandensis, B. mallei, B. pseudomallei, B. cenocepacia, or B. multivorans. The method includes administering an effective amount of a composition as described herein to an animal having or at risk of having a condition, or exhibiting symptoms and/or clinical signs of a condition, and determining whether at least one symptom and/or clinical sign of the condition is changed, preferably, reduced.

Melioidosis caused by B. pseudomallei tends to be more prevalent in people during the fourth and fifth decades of life, especially among those who have other comorbidities such as diabetes, alcoholism, immunosuppression, and renal failure. Melioidosis may be transmitted through direct skin contact with contaminated soil or water, ingestion of contaminated water, inhalation of dust contaminated with the bacterium, sexual contact, or intravenous drug use. Human-to-human transmission is rare but has been documented. The cutaneous form results in local infection with ulceration and swollen lymph glands. General symptoms include fever, rigors, night sweats, myalgia, anorexia, and headache. Additional symptoms are dependent on the route of exposure but may include chest pain, cough, photophobia, lacrimation, and diarrhea. Physical findings may include fever, cervical adenopathy, pustular skin lesions, hepatomegaly, or splenomegaly. During primary melioidosis, patients may experience severe urticaria. The chronic form may involve multiple abscesses that affect the liver, spleen, skin, or muscles, and may reactivate many years after the primary infection.

The pulmonary form of melioidosis may be manifest as pneumonia, pulmonary abscesses, and pleural effusions. Cutaneous abscesses may also develop and can take months to appear. Those who develop septicemia may develop respiratory distress, headaches, fever, diarrhea, pus-filled skin lesions, and abscesses throughout the body. Pustules often occur in association with regional lymphadenitis, cellulitis, or lymphangitis. Specifically, septicemia, high fever, and rigor are often present and may be accompanied by confusion, dyspnea, abdominal pain, muscle tenderness, pharyngitis, diarrhea, and jaundice. Although the foci of infection may be lungs or skin, once septicemia has developed, the disease spreads to the liver, spleen, kidney, brainstem, and parotid gland, leading to acidosis and shock with a high mortality rate exceeding 90% and death occurring within 24-48 hours.

B. mallei is the causative agent of glanders, which occurs primarily in horses and other solipeds. B. mallei is highly virulent and exhibits a pathophysiology in humans that is similar to glanders, where the clinical symptoms are similar to melioidosis as described above. Transmission is through direct skin or mucous membrane contact with infected animal tissues. Human-to-human transmission is possible and has been reported. Septicemia may include cutaneous, hepatic, and splenic involvement and is usually fatal within 7-10 days. The chronic form may involve multiple abscesses that affect the liver, spleen, skin, or muscles.

The Burkholderia cepacia complex is a group of at least 17 species responsible for opportunistic infections that are particularly problematic in diseases that cause impaired pulmonary function such as cystic fibrosis or chronic granulomatous disease. These organisms have also been a source of catheter-related infections in cancer patients and in those who are on hemodialysis. They have also been a source of skin and soft tissue infection, surgical wound infection, and genitourinary infection. The symptoms of pulmonary infection vary widely, ranging from asymptomatic infection to serious respiratory infections, especially in individuals that are immunocompromised, the young, the elderly, and people with lung disease. Symptoms are similar to other lung infections, with cough, wheezing shortness of breath, congestion, and fever. Thus, infection may be difficult to diagnose. Bacteria can persist in the lungs for years without symptoms.

Treatment of symptoms and/or clinical signs associated with these conditions can be prophylactic or, alternatively, therapeutic—in this context, treatment initiated after the subject exhibits one or more symptoms or clinical signs associated with a condition caused by infection by a gram negative microbe. As used herein, the term “symptom” refers to subjective evidence of disease or condition experienced by the patient and caused by infection by a microbe. As used herein, the term “clinical sign” or, simply, “sign” refers to objective evidence of disease or condition caused by infection by a microbe. Symptoms and/or clinical signs associated with conditions referred to herein and the evaluations of such symptoms are routine and known in the art. Treatment that is prophylactic—in this context, treatment that is initiated before a subject manifests symptoms or signs of a condition caused by a microbe—is referred to herein as treatment of a subject that is “at risk” of developing the condition. Thus, typically, an animal “at risk” of developing a condition is an animal present in an area where animals having the condition have been diagnosed and/or is likely to be exposed to a microbe causing the condition even if the animal has not yet manifested symptoms or signs of any condition caused by the microbe. Accordingly, administration of a composition can be performed before, during, or after the occurrence of the conditions described herein. Treatment initiated after the development of a condition may result in decreasing the severity of the symptoms of one of the conditions, or completely removing the symptoms. In this aspect of the invention, an “effective amount” is an amount effective to prevent the manifestation of symptoms of a disease, decrease the severity of the symptoms of a disease, and/or completely remove the symptoms. The successful treatment of a gram negative microbial infection in an animal is disclosed in Example 6, which demonstrates the protection against disease caused by B. thailandensis in a mouse model by administering a composition as described herein. These mouse models are a commonly accepted model for the study of human disease caused by these microbes.

This disclosure also provides methods for decreasing colonization by gram negative microbes, for instance blocking the attachment sites of gram negative microbe, including tissues of the skeletal system (for instance, bones, cartilage, tendons and ligaments), muscular system, (for instance, skeletal and smooth muscles), circulatory system (for instance, heart, blood vessels, capillaries and blood), nervous system (for instance, brain, spinal cord, and peripheral nerves), respiratory system (for instance, nose, trachea lungs, bronchi, bronchoceles, alveoli), digestive system (for instance, mouth, salivary glands esophagus, liver, stomach, large intestine, or small intestine), excretory system (for instance, kidneys, ureters, bladder and urethra), endocrine system (for instance, hypothalamus, pituitary, thyroid, pancreas and adrenal glands), reproductive system (for instance, ovaries, oviduct, uterus, vagina, mammary glands, testes, and seminal vesicles), lymphatic/immune systems (for instance, lymph, lymph nodes and vessels, mononuclear or white blood cells, such as macrophages, neutrophils, monocytes, eosinophils, basophils, and lymphocytes, including T cells and B cells), and specific cell lineages (for instance, precursor cells, epithelial cells, stem cells), and the like. Preferably, the gram negative microbe is a member of the family Burkholderiaceae, preferably, Burkholderia spp. such as, for example, B. thailandensis, B. mallei, B. pseudomallei, B. cenocepacia, or B. multivorans. Protein conservation suggests that the Burkholderia vaccines may also decrease colonization by other gram negative microbes such as Achromobacter spp., Ralstonia spp., Pseudomonas spp., Bordetella spp., and Acinetobacter spp.

Decreasing colonization in an animal may be performed prophylactically or, alternatively, therapeutically—in this context, initiated after the animal is colonized by the microbe. Treatment that is prophylactic—in this context, initiated before a subject is colonized by a microbe or while any colonization remains undetected—is referred to herein as treatment of a subject that is “at risk” of colonization by the microbe. Thus, typically, an animal “at risk” of colonization by a microbe is an animal present in an area where animals have been identified as colonized by the microbe and/or is likely to be exposed to the microbe even if the animal has not yet manifested any detectable indication of colonization by the microbe and regardless of whether the animal may harbor a subcolonization number of the microbe. Accordingly, administration of a composition can be performed before, during, or after the animal has first contact with the microbe. Treatment initiated after the animal's first contact with the microbe may result in decreasing the extent of colonization by the microbe, completely removing the microbe, and/or decreasing the likelihood that the animal becomes colonized by the microbe compared to an animal to which the composition is not administered. Thus, the method includes administering an effective amount of a composition as described herein to an animal colonized by, or at risk of being colonized by, a gram negative microbe. In this context, an “effective amount” is an amount sufficient to decrease colonization of the animal by the microbe, where decreasing colonization refers to one or more of: decreasing the extent of colonization by the microbe, completely removing the microbe, and/or decreasing the likelihood that the animal becomes colonized by the microbe compared to an animal to which the composition is not administered. Methods for evaluating the colonization of an animal by a microbe are routine and known in the art. For instance, colonization of an animal's intestinal tract by a microbe can be determined by measuring the presence of the microbe in the animal's feces. It is expected that decreasing the colonization of an animal by a microbe will reduce transmission of the microbe to humans.

A composition as described herein can be used to provide for active or passive immunization against bacterial infection. Generally, the composition can be administered to an animal to provide active immunization. However, the composition also can be used to induce production of immune products, such as antibody—e.g., a polyclonal antibody preparation or a monoclonal antibody—that can be collected from the producing animal and administered to another animal to provide passive immunity Immune components such as, for example, antibody can be collected from serum, plasma, blood, colostrum, etc. to prepare compositions (preferably containing the collected antibody) for passive immunization therapies. Antibody compositions including monoclonal antibodies and/or anti-idiotypes can also be prepared using known methods. Chimeric antibodies include human-derived constant regions of both heavy and light chains and murine-derived variable regions that are antigen-specific (Morrison et al., 1984, Proc. Natl. Acad. Sci. USA 81(21):6851-6855; LoBuglio et al., 1989, Proc. Natl. Acad. Sci. USA 86(11):4220-4224; Boulianne et al., 1984, Nature 312(5995):643-646.). Humanized antibodies substitute the murine constant and framework (FR) (of the variable region) with the human counterparts (Jones et al., 1986, Nature 321(6069):522-525; Riechmann et al., 1988, Nature 332(6162):323-327; Verhoeyen et al., 1988, Science 239(4847):1534-1536; Queen et al., 1989, Proc. Natl. Acad. Sci. USA 86(24):10029-10033; Daugherty et al., 1991, Nucleic Acids Res. 19(9): 2471-2476.). Alternatively, certain mouse strains can be used that have been genetically engineered to produce antibodies that are almost completely of human origin; following immunization the B cells of these mice are harvested and immortalized for the production of human monoclonal antibodies (Bruggeman et al. 1997, Curr. Opin. Biotechnol. 8(4):455-458; Lonberg et al., 1995, Int. Rev. Immunol. 13(1):65-93; Lonberg et al., 1994, Nature 368:856-859; Taylor et al., 1992, Nucleic Acids Res. 20:6287-6295.).

Passive antibody compositions and fragments thereof, e.g., scFv, Fab, F(ab′)₂ or Fv or other modified forms thereof, may be administered to a recipient in the form of serum, plasma, blood, colostrum, and the like. Antibody may, however, also be isolated from serum, plasma, blood, colostrum, and the like, using known methods for later use in a concentrated or reconstituted form such as, for instance, lavage solutions, impregnated dressings and/or topical agents and the like. Passive immunization preparations may be particularly advantageous for the treatment of acute systemic illness, or passive immunization of young animals that failed to receive adequate levels of passive immunity through maternal colostrum. Antibody useful for passive immunization also may be useful to conjugate to various drugs or antibiotics that could be directly targeted to bacteria expressing during a systemic or localized infection a polypeptide as described herein or a polypeptide having an epitope structurally related to an epitope present on a polypeptide as described herein.

Animal models, in particular mouse models, are available for experimentally evaluating the compositions as described herein. These mouse models are commonly accepted models for the study of human disease caused by members of the genus Burkholderia, and, in particular B. thailandensis, B. mallei, B. pseudomallei, B. cenocepacia, or B. multivorans. In those cases where a member of the genus Burkholderia causes disease in an animal, the natural host can be used to experimentally evaluate the compositions as described herein.

However, protection in a mouse model is not the only way to assess whether a composition can confer protection to an animal against infection by a Burkholderia spp. The adaptive immune response consists of two primary divisions: the humoral (antibody) response and the cellular (T cell) response. Following infection by a bacterial pathogen, dendritic cells at the infection site encounter microbial antigens and produce signaling molecules such as, for example, surface receptors and cytokines in response to conserved molecular patterns associated with the specific bacterium. These signals are shaped by the nature of the pathogen and ideally lead to the appropriate antibody and T cell responses that protect the host from disease. While some bacterial diseases are controlled primarily through antibody functions, others require T cell responses or both antibody and T cell responses for protection. The goal of vaccine biology is to identify the immune responses that provide protection and then design a vaccine to reproduce one or more of these responses in humans.

Antibodies can have many different functions in the conferring protection against infection such as, for example, complement fixation, opsonization, neutralization, and/or agglutination. Moreover, some subclasses of antibodies are better than others at specific functions; for example, for complement fixation the following hierarchy exists for human IgG subclasses: IgG3>IgG1>IgG2>IgG4.

Antibody immunological functions can be studied in a variety of ways. For instance, Western blots are used to identify antigen-specific binding based on size of separated proteins, while the standard enzyme-linked immunosorbent assay (ELISA) is used to produce quantitative information about antibody titers within serum. Antibody surface binding studies are used to determine whether antibody in serum are able to recognize antigens on the surface of intact bacteria, an important indicator of whether the antibodies have the potential to work in vivo. Thus, one skilled in the art recognizes that antibody binding assays such as a Western blot, ELISA (e.g., using human antisera), and/or surface binding correlate positively with the specifically-bound antigens providing immunological activity against infection by Burkholderia spp. However, one skilled in the art further recognizes that a lack of antibody binding in an assay such as, for example, a Western blot, ELISA, or surface binding assay does not mean that the assayed antigen fails to provide immunological activity against infection by Burkholderia spp.

For example, FIG. 7 and Table 8 provide data showing antibody titer (FIG. 7) and Western blot data for certain representative Burkholderia iron-regulated polypeptides.

Techniques such as opsonophagocytosis assays (OPA), in which antibody and complement-bound bacteria are combined with human or mouse phagocytes to determine levels of bacterial killing, are useful for studying antibody function. Positive OPA results correlate with vaccine-induced protection in a mouse model. (Stranger-Jones et al., 2006, Proc. Nati. Acad. Sci. 103(45):16942-16947). A similar oxidative burst assay can be used to assess the level of reactive oxygen species (ROS) by fresh human or mouse neutrophils following interaction with antibody and complement-bound bacteria.

In some cases, one can determine that a candidate polypeptide possesses cell-mediated immunological activity and, therefore, the candidate polypeptide may exhibit immunological activity in the absence of inducing the production of antibodies. (Spellberg et al., 2008, Infect. Immun. 76(10):4575-4580). Cytotoxic or CD8⁺ T cells primarily kill infected cells directly through various effector mechanisms, while helper CD4⁺ T cells function to provide important signaling in the way of cytokines. These T cell classes can be further subdivided based on the cytokines they produce, and different subclasses are effective against different bacterial pathogens. T cells are often studied by assessing their phenotypes with flow cytometry, where antibodies are used to visualize the levels of specific surface markers that enable classification of the T cells as, for example, a recently activated CD4⁺ T cell, a memory CD8⁺ T cell, etc. In addition, cytokines and other products of T cells can be studied by isolating the T cells from lymphoid tissue and re-stimulating them with cognate antigen. Following antigen stimulation the T cells produce cytokines that may be visualized by, for example, intracellular cytokine staining coupled with flow cytometry, or collecting the cell supernatants and using Luminex bead technology to measure 15-25 cytokines simultaneously.

For example, Table 9 provides cytokine recall response of spleen cells from mice immunized with representative Burkholderia iron-regulated polypeptides.

Thus, in addition to mouse models, those of ordinary skill in the art recognize that immunological activity commensurate with the methods described herein may correlate with any one or more of the following: Western blot data showing that serum from animals exposed to a Burkholderia spp. contains antibody that specifically binds to a candidate polypeptide, cell surface binding assays demonstrating that antibody that specifically binds to a candidate polypeptide specifically binds to a Burkholderia spp., opsonophagocytosis data, and cytokine induction.

Another aspect of the present invention provides methods for detecting antibody that specifically binds polypeptides as described herein. These methods are useful in, for instance, detecting whether an animal has antibody that specifically binds polypeptides as described herein, and diagnosing whether an animal may have a condition caused by a microbe expressing polypeptides described herein, or expressing polypeptides that share epitopes with the polypeptides described herein. Such diagnostic systems may be in kit form. The methods include contacting an antibody with a preparation that includes a polypeptide as described herein to result in a mixture. The antibody may be present in a biological sample, for instance, blood, milk, or colostrum. The method further includes incubating the mixture under conditions to allow the antibody to specifically bind the polypeptide to form a polypeptide:antibody complex. As used herein, the term “polypeptide:antibody complex” refers to the complex that results when an antibody specifically binds to a polypeptide. The preparation that includes the polypeptides as described herein may also include reagents, for instance a buffer, that provide conditions appropriate for the formation of the polypeptide:antibody complex. The polypeptide:antibody complex is then detected. The detection of antibodies is known in the art and can include, for instance, immunofluorescence or peroxidase. The methods for detecting the presence of antibodies that specifically bind to polypeptides as described herein can be used in various formats that have been used to detect antibody, including radioimmunoassay and enzyme-linked immunosorbent assay.

In another aspect, this disclosure provides a kit for detecting antibody that specifically binds polypeptides as described herein. The antibody detected may be obtained from an animal suspected to have an infection caused by a gram negative microbe, more preferably, a member of the family Burkholderiaceae, preferably, Burkholderia spp. such as, for example, B. thailandensis, B. mallei, B. pseudomallei, B. cenocepacia, or B. multivorans.

The kit can include at least one of the polypeptides as described herein (e.g., one, at least two, at least three, etc.), in a suitable packaging material in an amount sufficient for at least one assay. Optionally, other reagents such as buffers and solutions needed to practice the invention are also included. For instance, a kit may also include a reagent to permit detection of an antibody that specifically binds to a polypeptide as described herein, such as a detectably labeled secondary antibody designed to specifically bind to an antibody obtained from an animal. Instructions for use of the packaged polypeptides are also typically included. As used herein, the phrase “packaging material” refers to one or more physical structures used to house the contents of the kit. The packaging material is constructed by conventional methods, generally to provide a sterile, contaminant-free environment. The packaging material may have a label which indicates that the polypeptides can be used for detecting antibody that specifically binds polypeptides as described herein. In addition, the packaging material contains instructions indicating how the materials within the kit are employed to detect the antibody. As used herein, the term “package” refers to a container such as glass, plastic, paper, foil, and the like, capable of holding within fixed limits the polypeptides, and other reagents, for instance a secondary antibody. Thus, for example, a package can be a microtiter plate well to which microgram quantities of polypeptides have been affixed. A package can also contain a secondary antibody. “Instructions for use” typically include a tangible expression describing the reagent concentration or at least one assay method parameter, such as the relative amounts of reagent and sample to be admixed, maintenance time periods for reagent/sample admixtures, temperature, buffer conditions, and the like.

The present invention is illustrated by the following examples. It is to be understood that the particular examples, materials, amounts, and procedures are to be interpreted broadly in accordance with the scope and spirit of the invention as set forth herein.

EXAMPLES Example 1 Preparation of Burkholderia Metal-Regulated Polypeptides

Compositions derived from B. thailandensis E264 included novel polypeptides expressed under iron restricted growth. Master seed stocks were prepared by inoculating a single colony of strain E264 into 100 mL Tryptic Soy Broth (TSB, Difco Laboratories, Detroit, Mich.) followed by incubation in a shaking incubator at 37° C. and 400 rpm overnight. The culture was expanded by a 1/100 dilution into fresh TSB and incubated as before until it reached the mid-log phase of growth. The bacteria were pelleted by centrifugation at 5000×g, 4° C., for 10 minutes. The supernatant was decanted, and an equal volume of PBS was added to resuspend the pellet. The bacteria were pelleted by centrifugation as before and the pellet was resuspended in TSB containing 15-50% glycerol at one-tenth of the original culture volume. Stocks were frozen in aliquots of 100-1000 μL and stored at −80° C. Working seed stocks were prepared using the same procedure but with the initial inoculum obtained from a frozen master stock vial.

The iron-regulated polypeptide (IRP) composition was prepared by inoculating B. thailandensis from a frozen stock into 10 mL tryptic soy broth (TSB) supplemented with 200 μM 2,2′-dipyridyl (DP) (Sigma-Aldrich, St. Louis, Mo.). Iron-replete cultures contained 300 μM FeCl₃. Cultures were incubated at 37° C. on a shaker at 400 rpm. After 16-24 hours incubation, 10 mL of culture was transferred into 90 mL prewarmed TSB supplemented with either 200 μM DP or 300 μM FeCl₃ and incubated at 37° C. on a shaker at 400 rpm. After 16-24 hours, 90 mL of culture was transferred into 900 mL prewarmed TSB supplemented with either 300 μM FeCl₃ or 400 μM DP and incubated at 37° C. on a shaker at 400 rpm. After 16-20 hours the cells were harvested by centrifugation at 10,000×g for 20 minutes at 4° C., resuspended in PBS and centrifuged to obtain the final cell pellet. The cell pellets were weighed and stored frozen at −80° C.

The frozen cell pellet was thawed at room temperature and resuspended by the addition of 25 mL Tris-EDTA buffer (15 mM Tris-HCl, 3 mM EDTA, pH 8.5) per gram of pellet. The cell suspension was distributed into sterile 50 mL conical tubes at a volume of 35 mL/tube. Tubes were placed in a −80° C. freezer for a minimum of 30 minutes after which they were removed and thawed at 25° C.-37° C. The cells were disrupted by sonication (Branson, Danbury, Conn.) for 90 seconds on ice. The disrupted cell suspension was transferred to a sterile 40 mL round bottom centrifuge tube and centrifuged at 39,800×g for 20 minutes at 4° C. The soluble membrane fraction in the supernatant was transferred to a sterile 40 mL round bottom centrifuge tube, and 3 mL of 30% sarcosine (N-lauroylsarcosine sodium salt, Sigma-Aldrich, St. Louis, Mo.) was added to each tube. Tubes were incubated for 16-24 hours at 4° C. with rocking. The detergent-insoluble membrane fraction was pelleted by centrifugation at 39,800×g for two hours at 4° C. followed by removal of the supernatant. Pellets were dried by inverting the tubes for a minimum of five minutes. The pellets were resuspended in 75 μl PBS (pH 7.2).

A sample of the pellet was evaluated by denaturing SDS-PAGE using 10% gels stained with Coomassie Blue and imaged using a LI-COR Odyssey infrared imaging system (LI-COR Biosciences, Lincoln, Nebr.)(FIG. 1). The banding profile indicated that expression of polypeptides in the 64 kDa to 98 kDa region was increased when B. thailandensis was grown under iron restriction (lane 3, DP) relative to growth in the presence of iron (lane 2, Fe). This is similar to what has been observed for other Gram negative bacteria grown under iron restriction and corresponds to receptors that are involved in iron acquisition.

Example 2 Two-Dimensional (2D) Gel Analysis of Iron-Regulated Polypeptides

Two-dimensional PAGE separation of membrane extracts from B. thailandensis grown under either iron-rich or iron-depleted conditions was performed using an acidic polyacrylamide system with cationic detergent benzyldimethyl-n-hexadecylammonium chloride (16-BAC) for the first dimension and sodium dodecyl sulfate (SDS) for the second dimension (Hartinger et al., 1996, Anal Biochem 240:126-133). A 50 μg sample of membrane extract was solublized in 7.5 M urea, 10% 16-BAC (w/v), 75 mM DTT, and 0.05% pyronin Y, and electrophoresis was conducted using an 8.7% acrylamide gel with a 50 mM phosphoric acid running buffer. Electrophoretic separation in the 16-BAC phase was carried out at a current of 15 mA, from anode to cathode, at 4° C. overnight until the dye front migrated out of the gel. The gel was stained with 8250 Coomassie Blue. Each lane was excised and equilibrated through four changes of 0.1 M Tris, pH 6.8, with further equilibration in reducing buffer (75 mM Tris, 576 mM glycine, 0.3% SDS, 5% β-mercaptoethanol) for five minutes. Gel strips were overlaid onto the second dimension gel and fixed into place with 0.1% agarose. SDS-PAGE (5-16% gradient) separation was performed using a PROTEAN plus dodeca cell (Bio-Rad Laboratories, Inc., Hercules, Calif.) at 25 mA/gel until the dye front migrated out of the gel.

Separation in the first and second dimensions is dependent on molecular weight, hence, larger polypeptides appear in the upper right portion of the second dimension gel, and polypeptides of decreasing molecular weight appear on a diagonal toward the lower left portion of the gel (FIG. 2). The increased intensity of staining associated with iron restriction in FIG. 2B compared with extracts from cells grown in the presence of iron in FIG. 2A indicates that the expression of these polypeptides is upregulated under iron restriction.

Example 3 Identification of Iron-Regulated Polypeptides in the Extract

Extracts of B. thailandensis grown under iron depleted conditions were subjected to 2D gel electrophoresis as described in Example 2. Regions of the gel that stained positive for polypeptides were excised from the second dimension gel and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) according to the following procedure (Wilm et al., 1996, Nature 379:466-469). The excised gel spot was cut into smaller pieces and washed twice with water for 10 minutes each. All wash volumes were approximately equal to twice the volume of the gel pieces. The gel pieces were washed with a 1:1 mix of acetonitrile and 100 mM ammonium bicarbonate, pH 7.4, for 15-30 minutes. The wash was repeated once or twice as needed to remove the stain. The last wash was replaced with sufficient 100% acetonitrile (ACN) to cover the gel pieces until they turned opaque and sticky, whereupon they were removed and dried in a SAVANT SPEEDVAC (Thermo Fisher Scientific Inc., Waltham, Mass.) at 30° C. for 30 minutes. The dried gel pieces were placed into 25-30 μl of 50 mM ammonium bicarbonate containing trypsin at 110 ng/μl and digested for 16-18 hours at 37° C. Following digestion, the mixture was separated by centrifugation, the supernatant was removed, and a volume of 25-30 μl 0.1% trifluoroacetic acid (TFA) was added to extract the peptides. The samples were sonicated intermittently for 30 minutes, and supernatants containing the peptides were transferred into new tubes. The gel extraction was repeated using a solution of 0.1% TFA/30% ACN followed by 0.1% TFA/70% ACN. The pooled supernatants were concentrated in a SPEEDVAC to a final volume of 30-70 μl.

MALDI-MS analysis was performed using nano high-pressure liquid chromatography electrospray tandem mass spectrometry (nanoLC-ESI-MS/MS) methods with LTQ ORBITRAPS (Thermo Fisher Scientific Inc., Waltham, Mass.) coupled with NanoLC-2D pumps (Eksigent Technologies, LLC, AB SCIEX, Framingham, Mass.) for data acquisition and Scaffold analysis tool (Proteome Software, Portland, Oreg.) to compile the outputs from multiple search algorithms.

A polypeptide was considered to be present if at least two unique peptides for that polypeptide were identified in an excised gel spot. Fifteen polypeptides of interest were identified in the iron restricted extract (Table 2). Eight of the fifteen were detected only in the iron restricted extract and not in extracts from B. thailandensis grown in the presence of iron. These eight polypeptides are reflected in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:7, SEQ ID NO:16, SEQ ID NO:21, SEQ ID NO:22, and SEQ ID NO:25.

TABLE 2 Polypeptides identified in outer membrane preparations of B. thailandensis grown under iron restricted conditions. Molecular SEQ GI Weight ID # Polypeptide Number (kDa)^(a) 1 TonB-dependent siderophore receptor 83718630 81 2 RND efflux system outer membrane 83717404 55 lipoprotein 3 Outer membrane ferric siderophore 83716122 84 receptor 7 TonB-dependent copper receptor 83717103 81 9 OmpA family protein 83720098 27 11 OmpA family protein 83720431 24 12 Outer membrane porin 83719322 40 13 Outer membrane porin 83716488 44 14 Outer membrane porin 83718334 39 15 Outer membrane porin 83716254 55 16 RND efflux system outer membrane 83720933 58 lipoprotein 17 RND efflux system outer membrane 83718989 55 lipoprotein 21 Outer membrane porin 83718226 42 22 RND efflux system outer membrane 83719074 56 lipoprotein 25 RND efflux transporter MFP subunit 83720836 43 ^(a)Molecular weight (predicted).

Example 4 Differential Expression of Iron-Regulated Polypeptides in the Extract

The isobaric tags for relative and absolute quantification (iTRAQ) method was used to evaluate changes in expression of outer membrane proteins associated with growth of B. thailandensis in iron-depleted compared with iron-replete medium. Cultures were grown as described in Example 1, and 40 μg of outer membrane extract was evaluated. The primary amines of peptides and polypeptides were labeled with isobaric agents using ITRAQ-8plex reagents (Applied Biosystems, Life Technologies Corp., Carlsbad, Calif.) according to the manufacturer's instructions. Cation exchange chromatography was applied using an MCX column (Waters Corp., Milford, Mass.), and the peptides were separated using an UltiMate 3000 nano LC system (Dionex Corp., Sunnyvale, Calif.) coupled to ESI mode using a QSTAR XL mass spectrometer (Applied Biosystems, Life Technologies Corp., Carlsbad, Calif.).

The results are shown as the fold increase for a given protein in the iron restricted extract relative to the iron-replete extract (Table 3). A polypeptide was considered to be iron-regulated if it displayed a fold increase greater than 1 in at least two trials. Polypeptides identified as iron-regulated using iTRAQ analysis include those reflected in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, and SEQ ID NO:16.

TABLE 3 Detection and differential upregulation of B. thailandensis outer membrane polypeptides during iron-restricted growth. Molecular Fold increase by iTRAQ^(b) Weight (Trial No.) SEQ ID NO GI Number (kDa)^(a) (1) (2) (3) (4) 1 83718630 81 4.1 4.55 1.1 2.0 2 83717404 55 6.6 16.71 2.4 3.8 3 83716122 84 1.4 1.81 1.01 1.3 4 83717803 83 ND ND 1.5 1.8 5 83716062 81 ND ND 0.99 0.95 6 83720594 88 ND ND 1.2 1.2 7 83717103 81 ND 0.64 0.37 0.52 9 83720098 27 1.6 1.74 0.39 1.6 10 83719210 19 1.7 1.72 0.42 1.1 11 83720431 24 1.4 1.55 0.47 0.68 12 83719322 40 0.99 0.99 0.24 0.43 13 83716488 44 0.93 1.16 1.0 1.3 14 83718334 39 0.81 1.35 0.31 0.67 15 83716254 55 0.58 0.8 0.53 0.39 16 83720933 58 1.2 1.29 0.52 0.89 17 83718989 55 0.73 0.72 ND ND 18 83717287 19 0.18 0.17 0.17 0.14 20 83718178 36 2.7 ND ND ND 21 83718226 42 ND ND 0.45 0.46 22 83719074 56 ND ND 1.1 0.75 ^(a)Molecular weight (predicted). ^(b)Fold upregulation of polypeptides in extract vaccine from B. thailandensis E264 grown under iron-restricted compared with iron-replete conditions. A value of “ND” indicates that no peptides were detected.

Example 5 Identification of Additional Polypeptides Involved in Iron Uptake and Utilization, and Determination of Iron Regulation

A bioinformatics approach was undertaken to identify additional polypeptides that might be involved in iron uptake or utilization in B. thailandensis E264. Polypeptides of interest that had not already been identified as described in Examples 3 and 4 were downselected further to those shown in Table 4 based on structural similarity to known iron receptor proteins, localization in the bacterial outer membrane (computationally predicted by PSORTb V3.0), and additional information implicating them as iron receptors through comparison with B. pseudomallei orthologs. In addition, the polypeptide reflected in SEQ ID NO:19, which is involved in the acquisition and release of iron from intracellular storage by bacterioferritin, was also identified as a potential vaccine target.

TABLE 4 Additional B. thailandensis E264 polypeptides involved in iron uptake and utilization SEQ Molecular ID GI Weight NO: Polypeptide Number (kDa)^(a) 8 TonB-dependent siderophore receptor 83717123 81 19 Bacterioferritin-associated ferredoxin- Bfd 83718020 8.5 23 TonB-dependent receptor 83718695 85 24 TonB-dependent receptor 83717289 78 ^(a)Molecular weight (predicted).

The final determination of metal regulation for candidate proteins was based on mass spectroscopy, iTRAQ analysis, and bioinformatics described in Examples 3, 4, and 5, respectively. Each of the 25 polypeptides was determined to be either iron-regulated, non-iron regulated, or had iron regulation that was uncertain (Table 5). This determination was made using the following, step-wise inclusion criteria. First, any protein that was detected in mass spectroscopy of iron-restricted but not iron replete extracts was considered to be iron-regulated. Second, any protein that demonstrated a fold increase in expression of greater than 1 in iTRAQ analysis was considered to be iron-regulated. This resulted in a total of 14 polypeptides being classified as iron-regulated. Of the remaining proteins, four were considered to be non-metal-regulated if they were detected in at least two iTRAQ trials but did not demonstrate a fold increase greater than 1. Seven polypeptides could not be definitively categorized as either iron-regulated or non-iron regulated, and these were classified as polypeptides whose iron regulation was uncertain.

TABLE 5 Summary of iron regulation for 25 candidate proteins SEQ Molecular Iron ID NO: Weight (kDa)^(a) regulation^(b) 1 81 IR 2 55 IR 3 84 IR 4 83 IR 5 81 U 6 88 IR 7 81 IR 8 81 U 9 27 IR 10 19 IR 11 24 IR 12 40 NIR 13 44 IR 14 39 NIR 15 55 NIR 16 58 IR 17 55 U 18 19 NIR 19 8.5 U 20 36 U 21 42 IR 22 56 IR 23 85 U 24 78 U 25 43 IR ^(a)Molecular weight (predicted). ^(b)IR, iron regulated; NIR, non-iron regulated; U, iron regulation is uncertain

Example 6 Animal Efficacy Studies

Vaccine efficacy studies were conducted in BALB/c and A/J mice using 15 mice per group. Vaccines were formulated to deliver doses of 50 ng, 100 ng, and 300 ng of extract vaccine containing 10 ng/dose of CpG and emulsified in 50% incomplete Freund's adjuvant (IFA). The placebo group consisted of IFA/CpG alone. Mice were immunized subcutaneously in a volume of 100 μl at Day 0 with a booster at Day 28.

On Day 42, mice were challenged intratracheally with a previously determined LD80 of B. thailandensis E264, and survival was monitored for 14 days. The challenge dose of B. thailandensis E264 was prepared as follows. A loop of bacteria was taken from a frozen glycerol stock, placed into 10 mL Luria Bertani (LB) broth, and incubated at 37° C. overnight. The 10 mL culture was inoculated into 100 ml of LB, incubated at 37° C. and monitored for growth by optical density at a wavelength of 600 nm (OD₆₀₀). When the culture reached an OD₆₀₀ of 1.9 (approximately 1×10⁹ cfu/ml), the suspension was serially diluted with PBS to the predetermined challenge dose. All suspensions and dilutions were plated on LB agar to verify the actual concentration of bacteria administered.

Aerosolized challenge of mice was performed by intratracheal instillation according to an established protocol. In brief, mice were lightly anesthetized with a mixture of ketamine and xylazine (80 mg/kg ketamine and 20 mg/kg xylazine). The animal was manually restrained in an upright position and a padded forceps was used to gently open the mouth and hold the tongue down to the lower jaw to prevent swallowing. A second investigator then carefully administered 30 μl fluid to the back of the mouth using a sterile pipette tip and a p100 PIPETMAN (Gilson, Inc., Middleton, Wis.). This was followed by placing a gloved finger over the mouse nostrils to prevent obligate nasal breathing. The combination of holding the tongue to prevent swallowing and closing off the nostrils to prevent nasal breathing causes the mouse to inhale through the mouth and aspirate the instilled fluid. An immediate cough by the mouse, which can be detected both audibly and visibly, was used to verify that the procedure was performed correctly.

The results are shown in FIG. 3 and FIG. 4. In A/J mice, the 300 μg dose of vaccine resulted in 86% survival compared with 14% in the placebo. Lower doses of vaccine were not as protective. The increase in survival of vaccinated A/J mice, which are deficient in complement component C5, indicates that the vaccine is efficacious in animals that are predisposed to infection. Thus, the vaccine may also be effective in immunocompromised patients such as those with cystic fibrosis or diabetes. Protection in BALB/c mice was more robust, with increased survival demonstrated at all three doses of the vaccine. Compared with 13% survival in the placebo, vaccinates showed 67% and 60% survival at the 100 μg and the 50 μg doses, respectively, and 40% survival at the 300 μg dose.

Example 7 Conservation of Iron-Regulated Polypeptides

Because iron-regulated polypeptides tend to be evolutionarily conserved, the B. thailandensis extract vaccine would be expected to protect against infection by two of the most highly virulent species of Burkholderia, namely, B. pseudomallei and B. mallei. It may also protect against other Burkholderia such as those in the B. cepacia complex (BCC) that cause opportunistic infections in people with cystic fibrosis and chronic granulomatous disease. In this regard, B. cenocepacia (formerly BCC genomovar III) and B. multivorans (formerly BCC genomovar II) are two of the most common isolates from cystic fibrosis patients and are associated with increases in morbidity and mortality.

In the Example 6 lethal challenge studies, B. thailandensis was selected as a seed strain for the extract vaccine due to safety considerations associated with employing a BSL3 agent in manufacturing. However, recombinant polypeptide vaccines represent an alternative approach that is not subject to this limitation. Thus, a recombinant Burkholderia vaccine could employ polypeptides cloned directly from B. pseudomallei, which is highly lethal and difficult to treat, recognized as a potential bioweapon, endemic in certain parts of Asia and Australia, and considered to be an emerging infectious agent in other parts of the world. To address the possibility of whether a broad spectrum vaccine could be created using extract or recombinants, a bioinformatics approach was undertaken to compare the percent identity of the polypeptides identified in Examples 3, 4, and 5 across a variety of Burkholderia species and strains. Targeted vaccine polypeptides were compared by standard protein BLAST (blastp, NCBI) using a database of non-redundant polypeptide sequences and default parameters. To provide information on both the B. thailandensis extract vaccine and the B. pseudomallei recombinant vaccine, the analysis was performed using B. thailandensis E264 or B. pseudomallei K96243 polypeptides as the query against a subset of the sequenced Burkholderia strains available through GenBank. This panel of targeted strains was selected to achieve diversity based on geographic variability and differences in clinical disease and/or outcomes. Strains of particular interest to the Defense Threat Reduction Agency, as indicated in Broad Agency Announcements, were also included. Information on the selected strains and their sources is shown in Table 6.

TABLE 6 Names and sources of strains used to compare sequence conservation of iron-regulated polypeptides Species Strain Source B. K96243 Human, septicemia, fatal, Thailand, 1996 pseudomallei Pakistan9 Human melioidosis, Pakistan, 1988 1106a Human isolate from liver abscess aspirate, survived but relapsed, Thailand, 1993 1710a Human blood culture isolate (bacteriemia with lung and soft tissue involvement), Thailand, 1996 1710b Human blood culture isolate (second isolation from relapse of same patient as 1710a), fatal, Thailand, 1999 576 Human melioidosis, atypical LPS and highly virulent in mice, Thailand, 1989 Pasteur Human melioidosis, Vietnam or Thailand 52237 (from the Finkelstein collection) S13 Environmental strain, mucoid, Singapore 406e Human, lethal disseminated disease (bacteremia, lung, skin, and renal involvement), Thailand, 1988 MSHR668 Human blood culture isolate, severe melioidosis encephalomyelitis, Australia, 1995 MSHR305 Human brain, encephalomyelitis, fatal, Australia, 1994 1026b Human blood culture isolate, disseminated disease (bacteremia with skin, soft tissue, joint, and spleen involvement), Thailand, 1993 B. NCTC10229 Hungary, 1961 mallei ATCC23344 Human glanders/melioidosis, fatal, Burma, 1944 NCTC10247 Unknown passage history, avirulent in hamsters, Turkey, 1960 PRL-20 Horse blood isolate, Pakistan (Lahore Polo Club outbreak), 2005 ATCC10399 Horse lung isolate, China, 1942 GB8 horse 4 Horse-passaged derivative of ATCC23344 SAVP1 Natural infection, overseas isolate, severity likely mild B. E264 Environmental isolate, Thailand thailandensis MSMB43 Environmental isolate (water), Australia MSMB121 Environmental isolate, Australia B. AU1054 Human blood isolate, cystic fibrosis cenocepacia patient, epidemic isolate HI2424 Onion field soil isolate J2315 Human sputum isolate, cystic fibrosis patient, epidemic isolate, ET-12 lineage, United Kingdom-most studied MC0-3 Corn field soil isolate, United States, 2004 PC184 Human, cystic fibrosis patient, epidemic isolate, United States H111 Human, cystic fibrosis patient, Germany, 1993 B. ATCC17616 Environmental soil isolate, United States multivorans ATCC Human sputum, cystic fibrosis BAA-247 patient, Belgium CF2 Human sputum, United States CGD1 Human sputum, chronic granulomatous disease patient, United States CGD2 Human blood, chronic granulomatous disease patient, United States

Amino acid sequence comparisons for the polypeptides reflected in SEQ ID NO:1 through SEQ ID NO:25 are shown in FIG. 5A and FIG. 5B using B. pseudomallei K96243 or B. thailandensis E264 as the query, respectively. All strains of B. pseudomallei and B. mallei showed at least 98% identity with the B. pseudomallei K96243 vaccine strain. Burkholderia cenocepacia and B. multivorans were more divergent but still exhibited substantial homology that in some cases reached 90% or higher. A similar trend was noted using B. thailandensis E264 as the query. The high level of sequence identity with B. pseudomallei and B. mallei reflects their close phylogenetic relationship and suggests that B. thailandensis polypeptides would be capable of generating cross-reactive immune responses to these species. This may also be true for B. cenocepacia and B. multivorans, if there is sufficient conservation in regions of the polypeptides that are immunogenic.

To further evaluate the conservation of the iron-regulated polypeptides across multiple species, a cross-species alignment was performed for the polypeptides reflected in SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:9 using Clustal Omega (v1.2.0, European Molecular Biology Laboratory-European Bioinformatics Institute) and one strain from each of the five species. These three polypeptides were selected because they are representative of three different types of iron-regulated polypeptides, namely, a siderophore receptor (SEQ ID NO:1), an RND efflux system polypeptide (SEQ ID NO:2), and an OmpA family outer membrane polypeptide (SEQ ID NO:9), and they showed varying levels of identity across species. The strains used for comparison were B. thailandensis E264, B. pseudomallei K96243, B. mallei NCTC10229, B. cenocepacia AU1054, and B. multivorans ATCC17616.

The alignments indicate that certain regions of these three polypeptides are highly conserved across strains selected from all five Burkholderia species of interest (FIG. 6). The polypeptide reflected in SEQ ID NO:1 shows greater variability at the amino terminus, which is where the signal sequence, generally subject to cleavage, is located. This was also apparent though less dramatic for the polypeptide reflected in SEQ ID NO:2. The polypeptide reflected in SEQ ID NO:9 was highly conserved, although B. thailandensis E264 was somewhat unique in that it had additional amino acids at the amino terminus that were not present in any of the other species. Further examination of B. thailandensis strains revealed that MSMB43 also had additional amino acids at the amino terminus, though fewer than E264, whereas MSMB121 did not (not shown). Nonetheless, the remainder of this polypeptide was highly conserved.

Example 6 Immunogenicity of IRPs Vaccine Preparation

A comparative immunogenicity study was performed to evaluate the humoral and cellular immune responses elicited by the B. thailandensis IRP (iron-regulated polypeptide) extract vaccine, a purified subunit of the IRP extract vaccine, and a mixture of eight recombinant polypeptides cloned from B. pseudomallei K96243 (eight recombinant Burkholderia IRPs, rBIRP8, Table 7).

TABLE 7 Recombinant B. pseudomallei K96243 polypeptides SEQ Molecular ID GI Weight NO: Polypeptide Number (kDa)^(a) 51 Iron uptake receptor 53719389 83 52 Multidrug efflux system protein 53721329 55 53 Iron transport receptor protein 53722229 82 54 Exported heme receptor protein 53721278 82 55 Fe(III)-pyochelin receptor 53721627 82 56 Ton B-dependent outer membrane 53720334 81 receptor protein 57 Outer membrane copper receptor 53722763 80 58 TonB-dependent receptor family protein 53722050 88 ^(a)Molecular weight (predicted).

The extract vaccine used in these studies was prepared as described in Example 1, and the purified subunit vaccine was prepared with the following modifications. After breaking the cells, the soluble fraction was diluted 1:1 with 0.1 M sodium carbonate and mixed at 4° C. for one hour before continuing with centrifugation of the membrane fraction and sarcosine extraction. The insoluble extract product was subjected to a hot phenol extraction (Westphal et al., 1965, Methods in Carbohydrate Chemistry 5:83-91). In brief, the insoluble extract was resuspended in water and heated to 70° C. An equal volume of phenol was added, the temperature was re-equilibrated to 70° C., and the mixture was maintained at this temperature for 30 minutes. The phases were separated at room temperature using a glass separatory funnel. The phenol fraction and interface were combined and precipitated by the addition of methanol at a 3:1 v:v ratio. The polypeptides were recovered by centrifugation for one hour at 30,000×g and the pellet was washed with methanol. Methanol was removed through two sequential washes with a 50 mM Tris/1 mM EDTA buffer, and the final pellet was recovered by centrifugation for one hour at 45,000×g. The pellet was resuspended in a buffer containing 50 mM Tris, 1 mM EDTA, 7 M urea, 2 M thiourea, and 1% zwittergent by adding a volume of 1 ml per mg of pellet. The pellet was further solubilized by sonication at 60° C.-65° C. for 10 minute cycles interspersed with a 10 minute rest, followed by overnight incubation at room temperature on a rocking platform. The resulting product had an endotoxin level of <100 EU/mg as determined by a kinetic-turbidimetric Limulus amoebocyte lysate (LAL) assay (Charles River Laboratories, Wilmington, Mass.).

Eight IRP coding regions were cloned from B. pseudomallei K96243 DNA and expressed in E. coli (Table 7). The signal peptide sequence was predicted using SignalP (Center for Biological Sequence Analysis, Technical University of Denmark, Lyngby, Denmark) and, in some cases, PrediSi (Institute for Microbiology, Technical University of Braunschweig, Braunschweig, Germany) or Phobius (Stockholm Bioinformatics Centre, Stockholm University, Stockholm, Sweden). Gene-specific primers were designed to express the polypeptide without the signal sequence. PCR was performed using DNA Herculase II (Agilent Technologies, Santa Clara, Calif.), and the PCR products were ligated into the pQE30Xa expression vector (Qiagen, Valencia, Calif.), which adds a 6× Histidine tag to the N-terminus Ligation reactions were used to transform E. coli XL-1 blue. Clones were selected and verified by DNA sequencing (ACGT, Inc., Wheeling, Ill.). The recombinant B. pseudomallei iron-regulated polypeptides (rBIRPs) were expressed and purified using standard methods. In brief, frozen bacterial stocks (100 μl) were used to inoculate 20 ml of Luria-Bertani Broth containing 100 μg/ml of ampicillin, and the culture was grown at 37° C. in a shaking incubator at 250 rpm. After 16 hours, the culture was diluted added to 1 L of Luria-Bertani Broth containing 100 μg/ml of ampicillin, grown to an optical density (600 nm) of 0.6, and induced by the addition of 1 mM IPTG to a final concentration of 1 mM. Cultures were incubated for an additional 4-20 hours, depending on the optimum time for expression as previously determined for each clone. Bacterial cell pellets were harvested by centrifugation at 4,000 rcf for 20 minutes at 4° C., washed in PBS, and resuspended in 20 mM Tris buffer containing 100 μg/ml lysozyme. The cells were disrupted by sonication at 50% duty cycle and output (Branson Sonifier, Danbury, Conn.) for eight minutes on ice. The lysate was subjected to centrifugation for 10 minutes at 40,000×g at 4° C. to remove insoluble material. The soluble supernatants were processed by immobilized metal affinity chromatography (HisTrap FF 5 ml, GE Healthcare) to purify the histidine-tagged recombinant polypeptide, followed by anion exchange chromatography to increase the purity and remove endotoxin. Polypeptide concentration was determined using the BCA method (Thermo Scientific, Rockford, Ill.) and polypeptide purity was measured by SDS-PAGE and densitometry.

Immunization of Mice

BALB/c mice were vaccinated with 100 μg B. thailandensis E264 extract vaccine, 100 μg B. thailandensis E264 purified subunit vaccine (Example 6), or a mixture of eight rBIRPs (rBIRP8, polypeptides of SEQ ID NO:1-SEQ ID NO:8 at 10 μg each). Vaccines were formulated with 10 μg CpG per dose and emulsified in 50% IFA. The placebo group consisted of IFA/CpG alone. Each group contained a total of 5 mice. Vaccines were administered subcutaneously in a volume of 100 μl at Day 0 with a booster at Day 28. Mice were bled on Day −1 (one day before the initial vaccination) and Day 27 with a terminal bleed performed on Day 56. Blood was processed to obtain serum, which was stored at −80° C. Equal volumes of individual serum samples were pooled and evaluated for antibody production.

ELISA

IgG antibody titers to individual rBIRPs were determined by ELISA. In brief, 100 μl of polypeptide at 2 μg/ml, solubilized in 8 M urea, was added to each well of a 96-well EIA/RIA plate (Corning Inc., Tewksbuty, Mass.) and incubated overnight at 4° C. All remaining steps were performed at room temperature. The plate was washed three times with PBS wash buffer (PBS containing 0.05% Tween 20) followed by the addition of 200 μl/well sample buffer consisting of PBS containing 0.05% Tween 20 and 1% bovine serum albumin. After 90 minutes, the sample buffer was replaced with 100 μl/well PBS sample buffer. Serial ⅓ dilutions of the primary antisera were performed in the plate by the addition of 50 μl to the first row, mixing 10 times, and transfer of 50 μl to the next row. The plate was incubated for 90 minutes followed by three washes and addition of 100 μl/well of an HRP conjugated goat anti-mouse IgG, heavy chain specific antibody (Jackson ImmunoResearch Laboratories, Inc., West Grove, Pa.). After a 90 minute incubation, the plate was washed four times followed by the addition of 100 μl TMB/well (BioFX, SurModics, Inc., Eden Prairie, Minn.). Color was allowed to develop for 30 minutes, and the reaction was stopped by the addition of 100 μl stop reagent (BioFX, SurModics, Inc., Eden Prairie, Minn.). The absorbance was measured at a wavelength of 450 nm, and the titer was calculated as the inverse of the dilution corresponding to an absorbance of 1.0. Controls included a standardized primary serum included on each plate to monitor assay variability and wells that were uncoated to subtract background. The limit of detection for the assay was the inverse of the initial serum dilution (indicated as a dotted line on FIG. 7).

All eight rBIRPs were highly and equivalently immunogenic, eliciting antibody titers greater than 100,000 by ELISA (FIG. 7). This included the response to the polypeptide reflected n SEQ ID NO:8, which was identified solely through bioinformatics analysis (Example 5). Vaccination with the purified subunit vaccine also resulted in detectable antibodies to all eight rBIRPs, although the overall titers to the rBIRPS were lower than in mice immunized with rBIRP8. This indicates that all eight rBIRPs were present in the PSV which, having been derived from the extract, indicates they were also present in the extract vaccine. It also demonstrates that antibodies to the B. thailandensis E264 PS vaccine cross-react with rBIRPs from B. pseudomallei K96243, further highlighting the conservation of these polypeptides across species. Moreover, antibodies to the polypeptide reflected in SEQ ID NO:2 were detected at a titer of greater than 1×10⁴ in mice vaccinated with insoluble extract. The polypeptide of SEQ ID NO:2 was one of the polypeptides in the extract based on the iTRAQ analysis in Example 4, and the immune response to this polypeptide suggests it may have been involved in the protection observed in animal efficacy studies described in Example 6. Serum from immunized mice was antigen-specific, since neither preimmune serum nor serum from the placebo reacted with any of the eight rBIRPs.

Western Blot

Antibody specificity was further evaluated by western blotting. Each rBIRP was electrophoresed on a 4-15% TGX gradient gel and the gel was blotted onto a nitrocellulose membrane. All membrane incubations were performed on a rocking platform at room temperature. Membranes were incubated for 1 hour in Odyssey blocking buffer (LI-COR Biosciences, Lincoln, Nebr.) diluted 1:1 in Tris buffered saline. The blocking buffer was decanted and replaced with the primary antiserum diluted in Odyssey blocking buffer as above with the addition of 0.05% Tween 20. After one hour, the primary antiserum was decanted and the membrane was washed three times for 10 minutes each with TBS containing 0.05% Tween. The final wash was decanted and goat anti-mouse IgG (IR dye conjugate, LI-COR), diluted in the same buffer as the primary antibody, was added at the dilution recommended by the manufacturer. After one hour the membrane was washed three times as before, rinsed with TBS, and dried overnight in the dark. Blots were imaged on a LI-COR Odyssey imaging system.

The results from western blots paralleled those for the ELISA (Table 8). Antibodies to all eight rBIRPs were detected in serum from mice vaccinated with either rBIRP8 or the PS vaccine. The polypeptide of SEQ ID NO:2 was detected by serum from mice immunized with the extract vaccine, and similar to what was seen in the ELISA, this polypeptide appears to be highly immunogenic for eliciting an antibody response.

TABLE 8 Reactivity^(a) of antibodies to individual rBIRPs on Western blots. Polypeptide on blot (SEQ ID NO:) Serum source 1/Dilution 1 2 3 4 5 6 7 8 Preimmune 1000 − − − − − − − − Placebo 1000 − − − − − − − − Extract 1000 − + − − − − − − Purified 500 + + + ++ + + + + subunit 1000 − + − + − − − − rBIRP8 1000 +++ +++ +++ +++ ++ ++ +++ +++ ^(a)Reactivity was assessed as: − (not detected), + (low), ++ (moderate), or +++ (strong).

Antigen-Specific Cytokine Production by Spleen Cells

At termination of the experiment on Day 56, spleens were harvested, processed into mononuclear cell preparations, and cultured individually with 10 μg/ml of individual rBIRPs or the purified subunit. After 48 hours, the cell supernatants were harvested and frozen at −80° C. until assessment for cytokine production using a cytometric bead array kit (BD Biosciences, San Jose, Calif.) and flow cytometer (FACSCanto2, BD Biosciences, San Jose, Calif.), performed according to the manufacturer's protocol. The net production of cytokine for each polypeptide stimulus was calculated by subtracting the corresponding value for the placebo group.

TABLE 9 Cytokine recall response of spleen cells from mice immunized with iron-regulated polypeptide vaccines. Stim- ulus (SEQ ID Cytokine Production (pg/ml) NO:) Vaccine^(a) IL-2 IL-4 IL-6 IL-10 IL-17 TNF-α IFN-γ 1 Extract  2  —^(b)  2 —  1 — — PS^(c) — — — — — — — rBIRP8 19 2 14 — 35 11 19 2 Extract  4 —  4 — 13 —  2 PS  2 — — — 15 — — rBIRP8 43 5 200  — 491  260  480  3 Extract — —  2 — — — — PS — — — — — — — rBIRP8  7 1  5 — 10  3  4 4 Extract  1 — — —  2 — — PS — — — —  2 — — rBIRP8 15 1 26 — 57 41 43 5 Extract — —  1 — — — — PS — 1 —  4 — — — rBIRP8 26 4 38 — 101  30 94 6 Extract — — — — — — — PS — — — — — —  2 rBIRP8  7 2 220  — 251  405  624  7 Extract  2 — 23 —  6 —  5 PS  2 — — —  1 —  6 rBIRP8 10 4 229  — 215  211  329  8 Extract — —  2 — — — — PS — — — — — — — rBIRP8 20 — 11 — 56  6 20 Purified Extract  2 — 293  15  2 —  1 subunit PS  4 2 503  — 13 164  10 rBIRP8 — — 17 — — 10 — ^(a)5 mice were tested individually in each vaccine group ^(b)—, below the level of the placebo group (background) or below the level of detection for the assay. ^(c)PS, purified subunit

Recall responses to individual rBIRP polypeptides and the PS vaccine polypeptide mixture were observed in all three vaccine groups (extract, PS, and rBIRP8, Table 9). Negative control spleen cell cultures incubated with PBS did not exceed background levels in the placebo control and therefore are not shown. Mice immunized with the extract vaccine demonstrated low but measurable production of IL-2 and IL-17 in response to four of the eight rBIRPS (SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:7). In addition, the polypeptides reflected in SEQ ID NO:2 and SEQ ID NO:7 stimulated production of IFN-γ. The pattern of responses was similar though not identical for mice vaccinated with the extract or PS vaccines. Most notable was that both groups exhibited a strong IL-6 response to PS antigens. Mice immunized with rBIRP8 tended to exhibit stronger cytokine recall responses to individual rBIRPs than mice immunized with extract or PS vaccines, suggesting that responses to IRPs can be boosted by decreasing the complexity of the vaccine composition and increasing the amounts of targeted IRP antigens. Moreover, the Thl (IFN-γ, TNF-α)/Th17 (IL-17, TNF-α) type of response observed for rBIRP8, coupled with IL-2 production to promote proliferation and an undetectable IL-10 (regulatory) response, is intriguing since IFN-γ is generally associated with protection to intracellular pathogens, and IFN-γ,TNF-α, and IL-17 have been implicated in protective responses to Burkholderia infections.

Sequences

BtE264-400 ID* MW Protein GI# Protein Locus# SEQ ID NO: Sequence 401 81 83718630 YP_442936 26 1 ttaccagttg tatttcgccg tcgcgatcac ggtgcgctgg ttgccgtaca tgcacaccga  61 atccgactgg cagccggcca cgtagcggcg gttgaacagg ttcgtcgcat tgagcgcgaa  121 gcgccagttg cgcagctcgt agtgcagcgc cgcgtcgaac agcgtgtagc tcggcaccgt  181 cagcgagttg tcggcggcgc ccgccgccgc gctcatgtag cgcacgcccg cgccgacgcc  241 gaagcccgtg agcggcccgt tgcgccacgt ccagtcggcc cacagcgacg cgatctggcg  301 cgggcgcggc acgtcgacgg gccacttgtt cagcgtgttg tcattcgcct gcacgttctt  361 cacgtcctga tagacgtacg cggcgatcac cgacagctcg cgcgacaggt tgcccaccgc  421 gctcagctcg acgccgcgcg agcgcacctc gcccacctgc acgaacgtgc tgctcgtcgg  481 atcgttcgga ttgctcatcg cgacgttcgt ctggttgatc tggtagacgg ccgcgttcag  541 catcaggttc ttgcccggcg gctgccagcg caggccagcc tcgatctggc ggcccttggt  601 cggcgtcgcg agcccgccgc ccgcgagctt cacgccgatc tgcggattga acgacgtcga  661 atagctgagg tacggcgcga ggccgtaatc gccgagatag gtcaggccga cgcgcccgct  721 gaacgcgtgg tcgttctgct tgaagctcgc cgagttcgcg atgtcgtcct gcgacgtgcg  781 cgtccagtcc tgccggccgc cgagcgtgag cacccagcgc tgccacttga tctggtcctg  841 cacgtacagg ccgaaggcgt tgagcgtcgt cttcgtgtcg gtgcgcgggt acgcgttcgg  901 cccgctgaag atgtcggccg gaatcggcgt gtagacgggc cggtacaggt tcaggccggg  961 ccccttcgcg agccattcgc tgtcggtcgt cgtctgccgg ttgtagtcga agccgaacag  1021 cagtgtgtgg ctcaacgggc ctgtcgtgaa tttcgcctgc gcctggttgt cgacgtcgaa  1081 gcggctgtag ttgaactgga acaggcccgc gtagcgcgtc atcgtcgcca tcgtcgggtc  1141 cgcgccgtcg aggccgccgc cgtagacgga cgcgtcgtcg agcgccaggt gcatccagcg  1201 cacgttctgc cggaacgtcc acaccgggtt cagcttgtgc tcgaactgat agccgatcga  1261 ccattgcttc ttgcggtagt ggtcgaagtt cgcatcggcc gtgtacaggt cgtccgagat  1321 catgccgttc ggattcggca gcacggtgcc gcgcgacggc aggaagttgc tcgacgtgtc  1381 gccccagtcc tgcaggtacg tcgcggcgag cgtgagcgac gtgtttgcgt tcggctgcca  1441 cttgagcgac ggcgcgaacg acacgcgctg gtcggcgagc ggccccgtct gcgcgttgcc  1501 gtcgcggccg acgccgacga tccggtacga cagcgtgcca tccttgtcga tcttgtcgcc  1561 gatatcgaac atcagctgct tgcgcgcgta gttgcccacc tgcacgccga cctcgcgaat  1621 gcgctcgccg ttcgcgagct tgctctgcac gtcgacgatc gcgcccgggt cgccctgccc  1681 gtacagcacc gacgtcgggc cgcgcagcac ggcgatgctg tcgatcatgt acggatcgac  1741 gcgccagctc gacaggttga tcgtgttcgg cacctgcagc ccgtcgacga acacggtcgg  1801 cgtgaagccg cgcagcgccg cgtaccagtc cgagcggttg tccgatccgt aggtcgagaa  1861 gcccgggatg tagcggaacg cctgattgat gtcggtcgcg cccgtcgcct cgatctgctg  1921 cgcggtgacg atgttgatcg tctgcgggat ctcgacgatc ggcgtatcgg tcttcgtgcc  1981 cgtggtgctg cgccgcgcga cgaggccgac gctcgcgtcg cgctccgccc ccgccgacac  2041 cgagatcgcg ggcaacgtgc cgcccgccgc agtgtcccca ttcacctgac tgcccggctg  2101 ccgcgcgtct gcccccggcc gcaccgcctg tgcctgcgca tgactcgccg ccgcgcagaa  2161 cgccacgcct gccgcggccg cgatcgcacg cacgcgcgtg ctggttgccc actccat  1 1 mewatstrvr aiaaaagvaf caaashaqaq avrpgadarq pgsqvngdta aggtlpaisv  61 sagaerdasv glvarrsttg tktdtpivei pqtinivtaq qieatgatdi nqafryipgf  121 stygsdnrsd wyaalrgftp tvfvdglqvp ntinlsswrv dpymidsiav lrgptsvlyg  181 qgdpgaivdv qsklangeri revgvqvgny arkqlmfdig dkidkdgtls yrivgvgrdg  241 naqtgpladq rvsfapslkw qpnants1tl aatylqdwgd tssnflpsrg tvlpnpngmi  301 sddlytadan fdhyrkkqws igyqfehkln pvwtfrqnvr wmhlalddas vygggldgad  361 ptmatmtrya glfqfnysrf dvdnqaqakf ttgplshtll fgfdynrqtt tdsewlakgp  421 glnlyrpvyt pipadifsgp nayprtdtkt tlnafglyvq dqikwqrwvl tlggrqdwtr  481 tsqddiansa sfkqndhafs grvgltylgd yglapylsys tsfnpqigvk laggglatpt  541 kgrqieaglr wqppgknlml naavyqinqt nvamsnpndp tsstfvqvge vrsrgvelsa  601 vgnlsrelsv iaayvyqdvk nvqandntln kwpvdvprpr qiaslwadwt wrngpltgfg  661 vgagvrymsa aagaadnslt vpsytlfdaa lhyelrnwrf alnatnlfnr ryvagcqsds  721 vcmygnqrtv iatakynw  402 55 83717404 YP_440292 27 1 tcaacgtgcc gcgacgtcct gcttgttcgg ctcgcgcgaa ccgacggcca cgtcgtttcc  61 ccaaccgccg ccgagcgcgc gaatcaggtt gaccgtcgat accgcctgcg tgcccgtcag  121 ctgattcgac tgcaattgcg actcgagcac cgagcgctcg ctgtcgatca cctcgagata  181 gctgaccgcg ccttcctgat actgcgtgcg cgacagcttc gccgcgcgac gcgacgcgtt  241 gaccgcatcg ctctgcgcgc ggatctgatc gtcgagcaaa cgcagatcgg cgaggttgtc  301 ctccacttcg cggaacgcga cgagcacctg ctgccgatag ttcgcgactt cctcgtcgta  361 cttcgcgcgc gcctgcgcga cgcccgcgct gcggcgtccg ccgtcgaaga tcggcagcgt  421 gagcgccgtg cccgcgaacg gtccgagcag gaacgtgcgg ctcgaccaca ggaacagatt  481 gccgagcgtc gccgcttcat agccgaacga acccgtgatg tcgagcttcg ggaaatacgc  541 ggacttcgcc aggccgatgc gcgcgttcgc ggccatcatc gcgcgctcgg ccgccgcgat  601 gtccggccgc cgctcgagca gcgcggacgg caaccccgcc ggcacgcgca ccgcgaccgg  661 cacgatcggc gtttccttga acgagaaatc agcgggcgcc ttgccgagca ggatcgcgag  721 cgcgtgctcg gacgcggcgc gccgccgtgc gacgccgacc gagtccgcct gagcggtcgc  781 gagctcgttc ttcgcgcgcg acacgtcgag ctcgctgatg tcgccttcgc tgaagcgccg  841 ctgcacgagc ttcagcgcct cctcgcgcag ctcgaccgtg cggcggtaca gatcctgatc  901 ggaatcgagc cggcgcagct cgaagtagtt ctgcgcgacg tccgcctgca acgcgagctg  961 caccgagcgg aacagcgcct ggctctgcgc ttcgtccgca cgcgacgctt cgacgttgcg  1021 gccgacgcgg ccgaacagat ccgcctcgta cgacaccgtg ccctgcgcgc gccagagcgt  1081 cgcgttcgtc gggccgctgc cctgcggctg gaactgcgac gccggcgaca gcccctcgcg  1141 cgtcggcccg aagcccacgc ccacttgcgg gaaccactgc gagcgcgcgg cgcgggtcgc  1201 cgcgcgcgcc tgctcgacgc gcgccgccgc ggccttcagg ttctggttcg cggcgagcgc  1261 ctgcgtttcg agcgcatcga gcgccggatc gccgaatacg cgccaccatt cgccgcgatg  1321 cgcgtcgtcg gcgggctcgg ccgtcttcca cgtgccggcc tgctcgcccg gcgcgagcgc  1381 cggcgcttcc ttgaacgcgg cgggcgtcgc gacatccggc cgcctgtagt cgggcccgac  1441 cgcgcacgcg gcaagcagcg cgacgagcag cccgctcgcc gccgcgatct tcgcgacgcg  1501 cgcgatgcgt tcgttgatgt tggtctt tt at  2 1 mnktnineri arvakiaaas gllvallaac avgpdyrrpd vatpaafkea palapgeqag  61 twktaepadd ahrgewwrvf gdpaldalet qalaanqnlk aaaarveqar aatraarsqw  121 fpqvgvgfgp treglspasq fqpqgsgptn atlwraqgtv syeadlfgrv grnveasrad  181 eaqsqalfrs vq1alqadva qnyfelrrld sdqdlyrrtv elreealklv qrrfsegdis  241 eldvsrakne lataqadsvg varrraaseh alaillgkap adfsfketpi vpvavrvpag  301 lpsallerrp diaaaeramm aanariglak sayfpkldit gsfgyeaatl gnlflwssrt  361 fllgpfagta ltlpifdggr rsagvaqara kydeevanyr qqvlvafrev ednladlrll  421 ddqiraqsda vnasrraakl srtqyqegav sylevidser svlesqlqsn qltgtqavst  481 vnliralggg wgndvavgsr epnkqdvaar  403 84 83716122 YP_439399 28 1 tcagtagcgc gcgttgagcg tgacgaacgc cgagcggccc ggcgcgatcg acgcgtagtg  61 cgccggatag gcctgatcga agtacgtgcg gttgaacagg ttgttcacgt tgagccggac  121 gtcgagcttc ttgttgatcc ggtattgcgc catcgcgtcg aagcgccagt acgacggcac  181 cgcgcgcagg ttcgcggtgt cgccgaacac cttcgacatg tagaacgcgc cgccgccgac  241 cgtgaacttc ggcgtcacgt cgtagttcgt ccatagcgtg aagctgtgct tcggcgtgtt  301 cgggaactgg tggcccttgt ccgccgtgtt cttgccgttc tcgcgcagct cgctcttcag  361 gtacgtgtag ccgccgaaca cctgccacgc gcgcgtgagc cggccggaga agccgagctc  421 gagcccctgc acgcgcttgc tgccgaccat cgcgtactgg ttgttcggca gcgtgacgcg  481 cgcgttcgtc gtgtcgatct gaaagagggc ggccgtgagc gcgagcttgt cgtcgagcac  541 gttccacttg gtgccgagct cgatgctgcg gttcttctcc ggcgcgagct ggtcggcgtt  601 cgagccgacg ccgcctcggc ccggcgtgag cgattgcgtc tccgcgcctt cgccgagcag  661 cgcgccggcg ggcgtcgacg acgtcgcgta cgacgcatag atgctgccgt tgggcgccgg  721 cttgaacacg aggccgagct gccagttcac gagcgtgtcg tcgcgcgtgt aggtcttgcc  781 gccgttcgcg cgggtgtcgg tgaagcgggt cgagtagtcg tcgatgcgca cgccggcgtt  841 cacctgccaa tgcttgctca gctcgaccgt gtcgaagccg tagagcgatt tcgtcacggt  901 gcgcgcatgc gcgtagtcgt tgttgcgcct gatcgagccc gcccacggat cgttcgggtt  961 cggcgaccac aggctcgtgc agttgtagcc ggacgccgcg ccgattcctt gctggcagat  1021 cttgccggtg ccggtcgcga cgctgtacga atcgcgcttg ccccattcgc gcgacagctc  1081 gatgccggtc gtgaagctgt gcttgaacgg gcccgtgcgg aattcgccga acagctcggt  1141 ctggttcgcg atgctgttga tcgcgctgtt gcggttgttg ttgcgccgcc agacgcggcc  1201 gttgatcacg ttgccctggc tgtcgtcggg ctgcgtccag atgtagtcct gcgtcgattc  1261 cgtgtagcgc gtcgtgttgc gcaccgtcag cgacggcgtg atgtcgtgct cgatccgcag  1321 cgtgccgatg tccgacgtcg tcttgcggaa gtcgcggtcg acgaggccgt agaagttgtg  1381 ccgatcgacg ttcgccggat agatcgtgtc gacgttcgcc ggcttgttcg aggccgtgta  1441 gaagtacggg atgccgccgt cgggcaggtc gtcggtcgac aggtggtagt agctcgcggt  1501 cacgcgggtg ggcgtgccga gcccgaacgc gatcgacggc gcgacacccc agcgttc tt  1561 gttcaccgcg tcgcggcccg cgacgtcgtt gttgtggctc atcaggttca ggcgaaacgc  1621 cgcgtggtcg gcgaactgcc agttgccgtc cgcggtgaag cggcggtagc ggtcggtgcc  1681 gagccccgcg ctcgcctcgg ctgtcgtgcc gagatgcggc gccttcgtga cgaggttgat  1741 gctgccgccc gcgccgcctc ggccgccgta cgcgccgtcc gagcccttcg tgatctcgat  1801 gcgctcggtg ttgaagatct cgcgcgtcgt cgcgcccgtg tcgcgcatcc cgtcgacgaa  1861 caggctgccc tgcgtgtcgt agccgcggat gaacgggcgg tcgccgagcg gattgccgcc  1921 ttcgcccgcg ccgaacgtga tgccgggcac cgtgcgcagc gcctcggtga gcgtcgccgc  1981 gccgctgtcg tggatcagtt ccggcggaat cacggtgacg gatttgggcg tgtcgacgag  2041 cggcgcggtg aatttcaccg acgacgagaa gtccgccttg tagctgcgct cggcggcgcc  2101 cttgacctgg atcggcgcga gctccttgtc gtgatcggcg gttgccgcgg gcggcgcggc  2161 gtcctgcgcg aacgccgggc tcgcggcgag cacgctgcag agggtggtga attttccgag  2221 cttcaactcg tcggaacgcg acttcatcgg tggtatttcc tcgccaggtg tgactcgatc  2281 gggcgaagca acggccgctg catgatgcgt tccgcccaa  3 1 mggthhaaav aspdrvtpge eippmksrsd elklgkfttl csvlaaspaf aqdaappaat  61 adhdkelapi qvkgaaersy kadfsssvkf taplvdtpks vtvippelih dsgaatltea  121 lrtvpgitfg ageggnplgd rpfirgydtq gslfvdgmrd tgattreifn terieitkgs  181 dgayggrgga ggsinlvtka phlgttaeas aglgtdryrr ftadgnwqfa dhaafrlnlm  241 shnndvagrd avnnerwgva psiafglgtp trvtasyyhl stddlpdggi pyfytasnkp  301 anvdtiypan vdrhnfyglv drdfrkttsd igtlriehdi tpsltvrntt rytestqdyi  361 wtqpddsqgn vingrvwrrn nnrnsainsi anqtelfgef rtgpfkhsft tgielsrewg  421 krdsysvatg tgkicqqgig aasgynctsl wspnpndpwa gsirrnndya hartvtksly  481 gfdtvelskh wqvnagvrid dystrftdtr anggktytrd dtlvnwqlgl vfkpapngsi  541 yasyatsstp agallgegae tqsltpgrgg vgsnadqlap eknrsielgt kwnvlddkla  601 ltaalfqidt tnarvtlpnn qyamvgskrv qglelgfsgr ltrawqvfgg ytylkselre  661 ngkntadkgh qfpntpkhsf tlwtnydvtp kftvgggafy mskvfgdtan lravpsywrf  721 damaqyrink kldvrinvnn lfnrtyfdqa ypahyasiap grsafvtlna ry  404 83 83717803 YP_440327 29 1 gtgcatcaac ctctgctggc gcggcggccg cttcgcgccg cgctgttcgg ggccttcggc  61 ctctacgcgg cggccgcgcg cgccgcgggc gccgcctccg aacccgcggc cgccgcgccg  121 ccgtccgccg cgtccgcacc gcaagtgcga cacgcggcga gcgcggccac acgcggcaac  181 gcgcgcgccc tcgacccgat caccgtcacc gcgacgcgca ccgcgtcggc cgcgagccgc  241 accgcggcga gcgtatcggt aatcaccgat tcggacctcg aagaacagca ggccgacaac  301 atcaaggacg cgctgcgcta cgagccgggc gtcaccgtgc ggcgcaccgc gtaccggccg  361 gcgaacgccg cgctcggcgg cggccgcgac ggcgattcga gcatcaacat ccgcggcctc  421 gaaggcaacc gcgtgctgct gatggaagac ggcatccggc tgccgagcgc gttctcgttc  481 ggtccgctcg aagccggccg cggcgattac gccgatctcg acacgctcaa gcgcatcgag  541 atcctgcgcg gcccggcctc gtcgctgtac ggcagcgacg gcctgacggg cgccgtcaac  601 ttcatcacga aagatccgtc cgatctgctg tcgatccacg ggaagaagac ctatttctcg  661 ttgcggccga gctacgactc ggtcgaccgc agcgtcggcg cggccgtggc ggcggcgggc  721 ggcaacgacc gcgtgcaggg gatgctgatc gcatccggcc gccgaggcca cgaggtcgac  781 acgcgcggcg gcgacaattc cgcgagcacg aagcgcacga ccgcgaatcc ccaggatgtc  841 tacacggaat cgctgctcgg caagctgacg atcacgccga cgccacgcga cacgttcaag  901 ctcaccgccg agacggtgcg gcgccggatc gacacgaacg tactgtcggc gatcaatccg  961 ccgacgacgc tcggcctcac cgcgaacgac aagctcgagc gcaaccgcct gagcatcgac  1021 tacgatttcc gcgacgacgc gtcacgctgg ttccagaccg cgcacgtgca gctctactac  1081 caggaatcga aacaggatca ggacgcgttc gagacgcgcg gcggccgcct gcaatcgcgc  1141 tctcgctcga accattacag cgagcgcgcg ttcggcggct ccgcgttcgc cgagagcggc  1201 ttcgcgaccg ggccgctcgc gcacaagctg ctgtacggcg tcgacggcag catcgaccgc  1261 atcaagagcc tgcgtgacgg cgccgtcgcg agccccggcg agtcgttccc gaacaaggcg  1321 tttccggaca ccgactacac gctgttcggc gcgttcgtgc aggaccagat cggcttcggc  1381 aagctgctcg tcacgccggg cctacgcttc gacgcatatc ggctgagccc gagcgcgggc  1441 gatccgctct tcaccggcaa gacggtcagc tcgagcgatc acgagctgtc gccgcgcctc  1501 gcggtgctgt acgaggtgac gcccgcgctg atcccgtatg cgcagtacgc gcacggcttt  1561 cgcacgccaa cgcccgacca ggtcaacaac agcttctcga atccgatcta tggctataca  1621 tcgatcggca atccgaacct gaagcccgag acgagcgaca cgctcgaagc gggcctgcgc  1681 ggcacgctcg gcacggggta cgggccgctg cgctacggcg tcgccgcgtt cgccggccgc  1741 tatcggaact tcatcgcgca gcgcatgatc ggcggcagcg gccggccgaa cgatccgctc  1801 gtgttccagt acgtgaactt cacgaacgcg cgcatccacg gcttcgaagg gcgcgccgag  1861 tgggtgatgc ccaacggctt cacgctgaag accgcgatgg cgttcacgaa gggcacgacg  1921 cgggacaacg gcgcggcaag cgagccgctc gacacggtca acccgttctc cgccgtgttc  1981 ggcgtgcgct acgagccgag cgagcgctgg ttcgcgcagg ccgacctgct gttgcaggcg  2041 ggcaagcgcg gccgcgacgt gtcgtcggcc gcgtgccggg agaagacgtg cttcacgccg  2101 ccgtcgtcgt tcgtcgtcga tctgcgcggc ggctatcact tcaacaagca cgtgagcgcc  2161 tacctcggca ttcacaacct gttcgaccgc aaatactgga actggtcgga cgtgcgcggc  2221 atcgccgccg attcgaacgt gctcgacgca tacaccgctc cgggccgcag cgtcgcggtc  2281 agcatgaagg tggatttctg a  4 1 mhqpllarrp lraalfgafg lyaaaaraag aasepaaaap psaasapqvr haasaatrgn  61 araldpitvt atrtasaasr taasvsvitd sdleeqqadn ikdalryepg vtvrrtayrp  121 anaalgggrd gdssinirgl egnrvllmed girlpsafsf gpleagrgdy adldtlkrie  181 ilrgpassly gsdgltgavn fitkdpsdll sihgkktyfs lrpsydsvdr svgaavaaag  241 gndrvqgmli asgrrghevd trggdnsast krttanpqdv ytesllgklt itptprdtfk  301 ltaetvrrri dtnvlsainp pttlgltand klernrlsid ydfrddasrw fqtahvqlyy  361 qeskqdqdaf etrggrlqsr srsnhysera fggsafaesg fatgplahkl lygvdgsidr  421 ikslrdgava spgesfpnka fpdtdytlfg afvqdqigfg kllvtpglrf dayrlspsag  481 dplftgktvs ssdhelsprl avlyevtpal ipyaqyahgf rtptpdqvnn sfsnpiygyt  541 signpnlkpe tsdtleaglr gtlgtgygpl rygvaafagr yrnfiaqrmi ggsgrpndpl  601 vfqyvnftna rihgfegrae wvmpngftlk tamaftkgtt rdngaasepl dtvnpfsavf  661 gvryepserw faqadlllqa gkrgrdvssa acrektcftp pssfvvdlrg gyhfnkhvsa  721 ylgihnlfdr kywnwsdvrg iaadsnvlda ytapgrsvav smkvdf  405 81 83716062 YP_440017 30 1 tcagaactgc ccgcgcacgg tcagcatcac gttgcgcggc tccccatagc gattgttcca  61 gccgggctgg ctgagactct ggtagtaagt gcggtcgaac aggttgttga cgttgagcgc  121 ggcgctccat tgcctgtcgt agcgatagcc gagccgcacg ctcgcgagcg cgtagccgcc  181 ctgtcgcatc gtgacgccgt tcgacgccgc cgagaaatcg ctctgcacct gcacgccgcc  241 gccgatgctc cagcggcgtt cctgccacgg caggtcgtag ttcgtccaca agcgcagcag  301 gtggcgcggc gtgagctccg ccgcgaacgc gccgccgttg gcgacgtcct tcgcatagcg  361 gatcgtgtcg aacgtgtagc tcgcccacac gctccaccac ggcgtgatgc gcccgttcgc  421 ctcgaattcg aagccctggc tgcgcacgct gccgccgttc acgtagtagc agctccgccc  481 ggcgcatgga tgtgcggcat cgacctgcgg attgttatcg agatcgatgc gaaacgccgc  541 gagcgacacg ttgagcttgc cgcccgacag ctcgcccttg ataccggtct cgtaggtgcg  601 gcccttcacc ggcgtcagga tgctgccgtc ccaggtcggc ttggtctgcg gctggaacac  661 ttccgcatag ctcgcgtacc acgaccagtc gcgcgcgaca tcccagatca gcccgccata  721 cggcgtgaac tggcggcccg cgttgtagtg cgcgccgagg ctgtcctggc tccaccagct  781 cacgcgcccg cccagcacga gcgtgaccgg ctgcgcgagc ttgatgcggc cgaggccgta  841 gacgcccttt tgcgagatgt cgttctgcat gtcctgccgg tacggcccga tacccggctc  901 cggcacgctg tccggattcc agcggtacac gttcaccggc gtgccggcgc ccgccagcag  961 cggcgcgctg agctgcccgt tgctgctgct cgcgtaggtt gcgccgaaca gcagttcgtg  1021 cgcgagcccg aacgcgcgca ccgggccctg cacgttcgca tcgacgctgc ggctatagcc  1081 gctgaactgg tatgcgcctc ccatcagcgt gccgcccgca cccgtggccc gatcgatcgc  1141 gccgtacgag cccgcgtatt tcaggtcgga gcgcaccgcc tgatactcgc cgctcaccag  1201 tgccttccag ccgccgccga gcttctgctc gaccgagccg aacgcgcgcg tcgtatccca  1261 gtcgaagtgg ccccagccga cgtcgaggaa tgtcgatcgc gacaggccga ggctcgagcc  1321 gtcgcgcgcc atcggcacgc cggacatgtc gggcaccgac gcgaccgact ggtattgcgc  1381 gccgaccgtg acgagcgtgt cgcgcgtcac gtcgatctcg gcgatgccgt agatcgagcg  1441 cgtatcctgc ttcgcgcgat cgtaaaagaa gccgcggtcc tcgtacgcgg cgacgagccg  1501 cccgcgcacc gtgcccgccg ggttcagcgg cccgccgatg tcggcctgcg ctcggtagcg  1561 cccccaactg ccgatgctcg cgctcgcgtt cgcggcaaac tgatattgcg gccgcttgcg  1621 caccagattg acggtcgcgg ccggattgcc cgagccgtgc agcaagccgt tcgcgccgcg  1681 caggatctcc acccgctcgt acaccgacat gtcctgcggc gagctcgcca tgtcgccgag  1741 cacgacgggc acgccgtcga gctcgaacga atccaccttg aagccgcgca cgaagtacgc  1801 ggtcgtcagc agcacgtatg gctgcacggt cacgccggcc gactgctgca tcacgtcgtc  1861 gaggctgaac aggttctgct gctcgatccg ctcgcgcgtc gtcacactga ccgattgcgc  1921 aatctcccgc agcgcgagcg gcatcttgcc gacagtcgcg atcgacgggt cgtccgccgc  1981 atccttcgac gcgcttaccg aaatcgcctt caactcgccg ccggccggcc gtgcgccggc  2041 ggctcgctgc cgcgtgcctt cctgcacgct ctcgttcttt tgcgtgtcgc tcttcgcggc  2101 cgtgccggca agcgccgccg caccggggtg ggccgcgatc agcatgccca ggcaaagcga  2161 cgaacgccac cgccttcccc cgaaacgccc aaccggcgcg gccacgcgcc gcgatctctc  2221 aggtcccgcc acctcgtact gcat  5 1 mqyevagper srrvaapvgr fggrrwrssl clgmliaahp gaaalagtaa ksdtqknesv  61 qegtrqraag arpaggelka isvsaskdaa ddpsiatvgk mplalreiaq svsvttreri  121 eqqnlfsldd vmqqsagvtv qpyvllttay fvrgfkvdsf eldgvpvvlg dmasspqdms  181 vyerveilrg angllhgsgn paatvnlvrk rpqyqfaana sasigswgry raqadiggpl  241 npagtvrgrl vaayedrgff ydrakqdtrs iygiaeidvt rdtlvtvgaq yqsvasvpdm  301 sgvpmardgs slglsrstfl dvgwghfdwd ttrafgsveq klgggwkalv sgeyqavrsd  361 lkyagsygai dratgaggtl mggayqfsgy srsvdanvqg pvrafglahe llfgatyass  421 sngqlsapll agagtpvnvy rwnpdsvpep gigpyrqdmq ndisqkgvyg lgriklaqpv  481 tlvlggrvsw wsqdslgahy nagrqftpyg gliwdvardw swyasyaevf qpqtkptwdg  541 siltpvkgrt yetgikgels ggklnvslaa fridldnnpq vdaahpcagr scyyvnggsv  601 rsqgfefean gritpwwsvw asytfdtiry akdvanggaf aaeltprhll rlwtnydlpw  661 qerrwsiggg vqvqsdfsaa sngvtmrqgg yalasvrlgy rydrqwsaal nvnnlfdrty  721 yqslsqpgwn nrygeprnvm ltvrgqf  406 88 83720594 YP_441958 31 1 atgcaagcgg gacccgggtc ctcgcatgcc gatgatcggc gttcggccgc gcgcgcgatg  61 ccatcgaggt tgcgggagtc gacgagcagt gaacagagcg aagcgggcgg gaaacgttcg  121 gggccgcgtg gcgccgacga cagtggagcg tgcggcgtgc gctcgggcgg gggcggtgga  181 cgaggcaatc cgtcgatgag ccgggcgcag catgcaccgt cgcgccgtcg cgcgttcgag  241 cgcagcttcg cggcggcggc cgcccgcgcc gctcacggcg cgcctgccgc gcatggcccg  301 cacgcgtgcc tcgccgccgc gtcgcgtcgc tcgacggccg tcgagcgctg cgtcaccgcg  361 ctcgcgtgcg cggtgacggc gtccggcgcg ctcgccgccg acgcggaccc ggccgaatcc  421 gcgcgcgacc cgcatcgcga actgccgacc gtgcgcgtga cgagcgacgc ggcgcacgca  481 tcgccgctgt ccacgccgct cacggccggc tcgcgcctga agctcgcgag cctcgacatg  541 cccgcgagcg tcgaggcgat cacgtccggg cagatggccg cgcgcggcga tcgcacgatc  601 gtcgacgccg tcacgcgcgc gacgggcttc agcacggccg cggcgcccgg caacggcggc  661 acggcgctca gcgtgcgcgg cttcgccggc caggaatcgg tgacgacgct cgtcgacggc  721 gtgcgtctct accccggcgc gggcaccgtc acgttcccgt tctcgacgtg gtccgccgag  781 cgcatcgagg tgctgcgcgg gccggcgtcg gtgctgcacg gcgaaggggc gatcggcggc  841 gtcgtcgatg tcgtgacccg gcgcccgcgc cgcgaacgat cgacgacgct gcaggcgagc  901 atcggcacgc aaggcgaaaa gcgcgtggcg ctcgacacga cgggcgcgct cggcccgcgt  961 ctttcttatc gtttccatct gagcgacgag cgcacgcgcg gcttcgtcga gcgcggcgac  1021 gcgcacgcga cggccgtcgg cggcgcgctg aagctcgatg tcgattcgcg tttgtcgatt  1081 acgctcgatt acgactacgg ccgccagaag ccggcgacct atttcggcgt gccggcggcg  1141 aacggcgtgc tcgaccgcgc gctgcgcgag cgcaactaca acgtcggcga cgcgacgatc  1201 gcttaccacg atacctggac gcggcttgcc gcgacctatc gcgcgggcaa cggcgtgacg  1261 ctcgacgccc agctctacta tctcgcgacg cgccgccatt ggcgcaacgc ggaatcgtac  1321 gcgctcgacg cggcggcgcg caccgtcgcg cgcagcgact atctcgagat cttccatcgc  1381 gagcggcagt tcggcgagcg cttcaccgca cgcatcgatt cgcgcgtgtt cggccgcgcg  1441 aaccgcctcg tggtcggcgc cgagttcaac cagatcgcgt tcgacggcgc gaacaacgcg  1501 ccgtatcgcg gcgaatcgac cgtcgccgcc gccggcttcg atcccggcgc gttcgcgagc  1561 cccgatccga cgctgccgcg ctttcgcacg cgcacgcatc aggcggcggc gttcatcgag  1621 aaccggctcg aggtcctgcc gcggctcgcg tgggtgagcg ggctgcgtta cgaccatctg  1681 tcgtttcacc gcgacgatct ggtcgcgggc ggcgcgttcg acaagacgtt cgcgcacacc  1741 ggctggcgca gcgggctcgt ctacgagatc gcgccgggcc tgaccgcgta cgcgcaatac  1801 acgacgggcg cggagggcgt gggctcgctc gtcacgctgc cggcgtcgca ggcgaactac  1861 acgctcgcga ccgggcgcca atgggaagcg ggcgtcaagc acgagatcga cgacgctcgc  1921 gcgtactgga cgctcgccgt ctacgacatc gtcaagcgcg gcctcgtcag cgtcgacccg  1981 ctgaatccgg cgcgcgcgca gcagatcggc cgccagtcgt cgcgcggcgt cgaactcgcg  2041 ggcggcgtgc gtctgccggg cggcgtgacg atcgacgcaa acgcggcgtt gctgcgcgcg  2101 cgttacgacg cgttcggcca gcgcgtcggc gacacggtcg tgcagcgcgc gggcaacgtg  2161 ccgcacgaca tcgcgcggca gaccgcgaac ctgtggatcg gctgggcgtt cgcgccaggc  2221 tggcgcgcga atgcgggctt gcgttacgtc ggccgtcgct acggcgacga cgcgaaccgc  2281 gtgccggtgc cgtcgtacac ggtgttcgac gcgtcgctcg cgtggcaggc gacgcgcgac  2341 gtcggcctcg cgctctacgc gcgcaacctt gcgaaccgta cgtacgcggc gtcgacgtcg  2401 aacggcggcg cgcaatggct gctcggcccg tcgcgttcgg ccgagctcgt cgcgacgctg  2461 cgcttctag  6 1 mqagpgssha ddrrsaaram psrlrestss eqseaggkrs gprgaddsga cgvrsggggg  61 rgnpsmsraq hapsrrrafe rsfaaaaara ahgapaahgp haclaaasrr stavercvta  121 lacavtasga laadadpaes ardphrelpt vrvtsdaaha splstpltag srlklasldm  181 pasveaitsg qmaargdrti vdavtratgf staaapgngg talsvrgfag qesvttlvdg  241 vrlypgagtv tfpfstwsae rievlrgpas vlhgegaigg vvdvvtrrpr rersttlqas  301 igtqgekrva ldttgalgpr lsyrfhlsde rtrgfvergd ahatavggal kldvdsrlsi  361 tldydygrqk patyfgvpaa ngvldralre rnynvgdati ayhdtwtrla atyragngvt  421 ldaqlyylat rrhwrnaesy aldaaartva rsdyleifhr erqfgerfta ridsrvfgra  481 nrlvvgaefn qiafdganna pyrgestvaa agfdpgafas pdptlprfrt rthqaaafie  541 nrlevlprla wvsglrydhl sfhrddlvag gafdktfaht gwrsglvyei apgltayaqy  601 ttgaegvgsl vtlpasqany tlatgrqwea gvkheiddar aywtlavydi vkrglvsvdp  661 lnparaqqig rqssrgvela ggvrlpggvt idanaallra rydafgqrvg dtvvqragnv  721 phdiarqtan lwigwafapg wranaglryv grrygddanr vpvpsytvfd aslawqatrd  781 vglalyarnl anrtyaasts nggaqwllgp srsaelvatl rf  407 81 83717103 YP_438837 32 1 atgacaatca aattcctgcg tcacgcgcac cccgcgcgtg tcgaccgtgc gcgccgtcgg  61 cggcgcgcga tcacgctcac cgttcccgcg ctcgcggcgg gcgcctttca tctcgcgccg  121 gccgtcgcgc agacggacga tgccgtgcac cgtcacggcg cactcggcgc gacgagcggc  181 gtcgcgaacg acgcggcacg cggcgcatcg agcgccaaat cgaacgctag ggcaaacgaa  241 gcgaccgccg tgacgcgcag cgcgacgaac cgcgcgacag ccggcgccgc gccggaagca  301 gcgatgctgc cgacgatcga gatcgtcgcg gcgcccgaat cgacgccgct cgtcgtcgtc  361 accgatccga aaacgccgcg ccagccgttg cccgcgagcg acggcgccga ttatctgaag  421 acgattcccg gcttcgcgtc gatccgcagc ggcggcacga acggcgaccc ggtgctgcgc  481 gggatgttcg gctcgcggct gaacatcctc gcgaacggca tgccgacgct cggtgcgtgt  541 cccggccgga tggacgcgcc gacgtcgtac atcgcgcccg agagctacga caaggtgacg  601 gtcgtcaagg gcccgcagac cgtgctgtac gggccgggcg catcggcggg cacggtgctg  661 ttcgaacgcg tgacgccgcg cttcaagacg ccgggcatgc gcttcgacgg cagtgtcgtc  721 ggcggctcgt tcgggcgcaa cgatcagaac gtcgacgtga cggcaggcac gcccgacttc  781 tacgggcgcg tgatcgcgaa ccatgcgcat tcgcaggatt acgaagacgg caacggtcgc  841 acggtgccgt cgcaatggga caagtggaac gcggacgcgg cgctcggctg gacccccgac  901 gacaacacgc ggctcgagtt gacggcgggc acgggcgacg gctacgcgcg ctacgcgggc  961 cgcgggatgg acggtgcaca tttccggcgc gagacgttcg gcctgacgtt cgacaagaag  1021 cacatcggcg acgtgctcga tcgcatcgag gcgcaggtct tctacaacga agccgatcac  1081 gtgatggaca actacacgtt gcggatgccc gatccgacga gcagcatgcc gatgcgcatg  1141 gcctccgaag tgcgccgccg cacgctcggc gcgcgcgtcg cggcgacgct gcgcttcacc  1201 gacgcgttca agctcgtgac gggcgtcgac gcgcagtcga accgtctcga ttcgcgctcc  1261 gcgatgggga tgcagaacta cggtgacaag ccgtggaatc cgcaggcgaa catgtggaac  1321 gcgggcgcgt tcggcgagct gacctggtat gcgagcgatg cgtcgcgcgt gatcggcggc  1381 gcgcggatcg actatgcggc cgcgcgcgac aagcgcgcga cgacgggcgg catgaagatg  1441 agcatgcgca atccgacgtt cgacgatctc cgctcgcgcg tgctgccgag cggcttcgtg  1501 cgctacgagc gcgatctcgc ttcgctgccc gtcacgtggt atgcgggcat cggacacgcg  1561 caacgctttc ccgattattg ggaactgttc tcggcgaagc gcggcccgaa cggcgccgtc  1621 aacgcgttct cggcgatcaa gcccgagaag acgacgcagc tcgacatcgg cgcgcagtac  1681 aagagcgaca agctcgacgc gtgggtgtcc gcgtacgcgg gctacgtgca ggacttcatc  1741 ctgttcgact acgcgacggg ctcgatggga cagacgacgc aggcgacgaa cgtcaatgcg  1801 cagatcatgg gcggcgagct cggcgcgtcg tggcgtccgc tcgcgccgtg gcgtttcgat  1861 gcgtcgctcg cgtatgcgtg ggggcgcaac gtgcaaagcg gcgcgccgct gccgcagatg  1921 ccgccgctcg aagcgcgttt cggcgtcgag tacacacgcg ggccgtggtc ggcgggcggg  1981 ctgtggcgaa tcgttgcgcc gcagcatcgc tatgcgctga acgagggcaa cgtcgtcggc  2041 aaggacttcg gtccgagcgc cggtttcggc gtgctgtcgc tgcacgcgca gtacaacgtg  2101 agcaaagcgg tgcagatctc ggtcggcatc gacaacgtgc tcgacaaggc ttatgcggag  2161 cacctgaacc tcgccggcaa cgccggtttc ggctatccgg cgaacatgcc cgtcacggaa  2221 cccggccgca cagcgtgggt tc tttgagc accaagctct ga  7 1 mtikflrhah parvdrarrr rraitltvpa laagafhlap avaqtddavh rhgalgatsg  61 vandaargas saksnarane atavtrsatn ratagaapea amlptieiva apestplvvv  121 tdpktprqpl pasdgadylk tipgfasirs ggtngdpvlr gmfgsrlnil angmptlgac  181 pgrmdaptsy iapesydkvt vvkgpqtvly gpgasagtvl fervtprfkt pgmrfdgsvv  241 ggsfgrndqn vdvtagtpdf ygrvianhah sqdyedgngr tvpsqwdkwn adaalgwtpd  301 dntrleltag tgdgyaryag rgmdgahfrr etfgltfdkk higdvldrie aqvfyneadh  361 vmdnytlrmp dptssmpmrm asevrrrtlg arvaatlrft dafklvtgvd aqsnrldsrs  421 amgmqnygdk pwnpqanmwn agafgeltwy asdasrvigg aridyaaard krattggmkm  481 smrnptfddl rsrvlpsgfv ryerdlaslp vtwyagigha qrfpdywelf sakrgpngav  541 nafsaikpek ttqldigaqy ksdkldawvs ayagyvqdfi lfdyatgsmg qttqatnvna  601 qimggelgas wrplapwrfd aslayawgrn vqsgaplpqm pplearfgve ytrgpwsagg  661 lwrivapqhr yalnegnvvg kdfgpsagfg vlslhaqynv skavqisvgi dnvldkayae  721 hlnlagnagf gypanmpvte pgrtawvrls tkl  408 81 83717123 YP_439577 33 1 tcagaactgc agcgtcgtca gcagcgacac ctgccgcgca tcgccgaccg acacgaagaa  61 ccggttcacg ctcgacgggt aatacgtgcg gttgaacagg ttcttcacgt tcagctggaa  121 ctgcagcttc tgcttgccga cgcgcgtgtc gtaggtcgcg aaagcatcgg ccgtcgcgta  181 cgcgggcagc gtgaagctgt tcgccgaatc gcccggccgc gcgccgacgt agcgccccgc  241 cgcgccgatc cgcaggtcgt cgccgccgaa cacggcgccc gcgtcgtaga cggccgcgag  301 cgacgccgtg tgccgcgcga cgttccacag ccggttgccc gcgtacagcg gatcttcggt  361 cgtcttcgca tcgatgtacg cgtagctcgc gatcacgttc cagcgctcgc cgatccgccc  421 cgacacgtcg agctcgatcc cgcgcgagcg cgcgcgtccg gacgtgcgcc agtcggtctg  481 gttcgtcgcg tcgttgtatt gcgacacgag cacgttcttc ttgtcgatct cgaagaacgc  541 gagcgtgccg gcgaagccgc tcggcaggtc gagcttcgcg ccgagctccc acgacgcgcc  601 ctgctcgggc gcggtcgagc cgtcgatcac gtagccgccc gacatcggcg cgatcttcga  661 cgtcggcttc agcgactgcg tgtagctgcc gtacagcgac agcgcgtcgt tccacttgta  721 gacgatgccc gcgcgcggca gccacttcgt gccgctcagg ttcgtgttga cccggaacgg  781 ccggccgcgc ccggcgagct ggctgtagcg gacgaagcgc gcgccgccga cgagaatcca  841 cttgtcggtc agatggatgc tgtcctggaa gaacagcgac gcggtgtgca gcgtgtcgct  901 ctgatcgctg tcggacgccg atacgctcgt cgacggcggg atcagcccgt acgtcgggtt  961 caggtagctg aacggcgtct tgatcgcctg gcgcagcatg tccgcgcggt agacctgccg  1021 gtattcgctg tcgacgccga actgcacgtc gtgccgcatc ccggcgagcg tcacgcggcc  1081 gtcgacgtat gcgattgcgt agctgtcggt gctgcgcgag ccgtgcgtcg cgtcgttgct  1141 gcgcgtgagc gtgcccttga ccggatcgac cgccgtgatg cgtatctggt tcgcatcgta  1201 ggtctcgcgg ttgtagctgt agccgaggtg cgccttccag ttgggcgcga gctgatggtc  1261 gaccgttagt tgcaccagat tcgattcgcc gcgcatgtcg ttgaacggct cgtcgaggcg  1321 gcggcgcgcc gggatcgcga gcggctcgtt cgtgcgcgga tcgagcgcgg tgccccgatc  1381 gaacggcatc aggaagcgcc ggtactcgta cgacagcacg acctgcgtgt cgcggccgta  1441 ccacgcgagc gacggcgcga cgagcgtctc gcgatgctcg ccgtagtcgc gccagtactg  1501 ctcgttcgtc tgatcgacga tcagccggta cgcaagccgc gaatcgccga tcgcgcccgt  1561 cgagtcgaac gtgagctcgc cgccattcct gccgtgcccg taggtcgagc cgagcaccga  1621 gatcgcgttg tggcgcgtga gctgcggctg cttcgtgacg acgttgatca cgccgcccgg  1681 gtccatgatc ccgtacagca gcgaagccgg ccccttcagc acttcgacgc tgtccgtcgt  1741 cgcgttcagc gagcggccct gcacgatcgg catcccgttg cgcatcaccg agccgtcgcg  1801 gttgtcgccg aagccgcgct tcatcaccgt gtcctgcgtg ctgccgagcg tgttgccctg  1861 cgtgatgccg ctcacgttcg cgagcgcgtc gtcgaggttg cgcgggcgct ggtcgcgcag  1921 cacctgcggc gcaacgatgt tgacggcctg cggcacctcg gcgagcggcg cgtcgctgcg  1981 cagccccgcc gcctcgcgcg gcgcacggta gctgtccgcg tgcaggccgc tcgcgcgcac  2041 cgagacggtc ggcagcgtcg tgtcggtcgc gagcgccgcc cccgcgctcg gcgcggccgg  2101 cccggcgggc gacctgacga gcgtgtagcc gccgctcggc tgccgcagcg cgacgaggcc  2161 cgtgcccgtc agcagccggt cgagcgccgc ggccgcatcc gcgcgaccgt gcaggccggg  2221 gctttgcaa  8 1 mqspglhgra daaaaldrll tgtglvalrq psggytlvrs pagpaapsag aalatdttlp  61 tvsvrasglh adsyraprea aglrsdapla evpqavniva pqvlrdqrpr nlddalanvs  121 gitqgntlgs tqdtvmkrgf gdnrdgsvmr ngmpivqgrs lnattdsvev lkgpasllyg  181 imdpggvinv vtkqpqltrh naisvlgsty ghgrnggelt fdstgaigds rlayrlivdq  241 tneqywrdyg ehretivaps lawygrdtqv vlsyeyrrfl mpfdrgtald prtneplaip  301 arrrldepfn dmrgesnlvq ltvdhqlapn wkahlgysyn retydanqir itavdpvkgt  361 ltrsndathg srstdsyaia yvdgrvtlag mrhdvqfgvd seyrqvyrad mlrqaiktpf  421 sylnptygli ppstsvsasd sdqsdtlhta slffqdsihl tdkwilvgga rfvrysqlag  481 rgrpfrvntn lsgtkwlpra givykwndal slygsytqsl kptskiapms ggyvidgsta  541 peqgaswelg akldlpsgfa gtlaffeidk knvlvsqynd atnqtdwrts grarsrgiel  601 dvsgrigerw nviasyayid akttedplya gnrlwnvarh taslaavyda gavfggddlr  661 igaagryvga rpgdsansft lpayatadaf atydtrvgkq klqfqlnvkn lfnrtyypss  721 vnrffvsvgd arqvsllttl qf  409 27 83720098 YP_441413 34 1 ttactgcgcg gcttgcggtg cgcgcaggta gatctcgacg cggcggtttt gcgcgcggcc  61 ggcttccgtc gcgttgtcgg cgatcgggtt cgacgcgccc atgccttgcg ccgacaggcg  121 ggttgccgcg acgccgcgtt gcgcgagcgc gttcacgacg ctttgcgcgc gattctgcga  181 cagcgtctgg ttgtgcgcgg ccgagcccgt gctgtcggtg tagccgacga ccgatgcggt  241 gatctgcggg ttctggttca gcgtcgtcgc gaggtcgttc aggagcggcg tgaaggccgg  301 cgtgaccgcg tactggtcgg tcgcgaacgt gaccgagctc ggcacgttca gcttgagcga  361 gccgtccggc tgctcggtga cctgcgtgcc cgtctgctgc gccgacggcg cgagcttgtt  421 cttgatcgct tgccagttgt aacccgtcac gccgccgacg agcgcgccga cgccggcgcc  481 gatcgccgcg cccttgccgc cgccagccag cgcgccgatg cccgcgccga gtgcggcgcc  541 cgtgccggtg ccgaccgccg tgttcgtgcc ttgctgggtt gcgcaacccg ccagcagggc  601 gccggcgaga gcgaaaacgg acaagcgcgt cgcgattttg gtattcatct tggtttcctc  661 tcttgagtga ctcgacaaga acaaaaaaga actgctcttt cggcgggggc gacccgcgcg  721 ccgacgatca tgcaggcgcg cgagcgcctc gatccgtccg gcgtcaggtg ccggggccgt  781 cttcat  9 1 mktapapdag riealarlhd rrragrprrk sssflflssh sreetkmntk iatrlsvfal  61 agallagcat qqgtntavgt gtgaalgagi galagggkga aigagvgalv ggvtgynwqa  121 iknklapsaq qtgtqvteqp dgslklnvps svtfatdqya vtpaftplln dlattlnqnp  181 qitasvvgyt dstgsaahnq tlsqnragsv vnalaqrgva atrlsaqgmg asnpiadnat  241 eagraqnrrv eiylrapqaa q  410 19 83719210 YP_441918 35 1 ttactgttga tagacgaggt ccgcgcggcg gttctgcgcc cacgacgctt cgtcgtggcc  61 gagcgcgacc ggcttttcct tgccgaggct cacggcttcc atctgcgaat ccccgacgcc  121 gagcagcgac agtgcgcgac gcaccgcttc cgcgcgcttc tggccgagcg cgaggttgta  181 ctcgctcgtg ccgcgctcgt cggtgttgcc ctggatcagg atgtggcgct gcgggtggct  241 cttcaggtat tgcgcgtgtt gctgcagcag cggctggtac tggtcctgca ccgagtagct  301 gtcgaaatcg aagtagacgc tgcgcttcgc gagcgggctg ttcggatcgt tcagcggatc  361 gacggtcact tgcgcgacgt tctcgggatt cggttgcgtg ctgacggcgc cgccctggtt  421 cgcgtgttcg tcgagcttca cccccgactt gcacgcggcg agcgcgccga tcatcagcat  481 cgcgaacgcg agacgaagtt ttttcgacat cat  10 1 mmskklrlaf amlmigalaa cksgvkldeh anqggavstq pnpenvaqvt vdplndpnsp  61 lakrsvyfdf dsysvgdqyq pllqqhagyl kshpqrhili qgntdergts eynlalgqkr  121 aeavrralsl lgvgdsqmea vslgkekpva lghdeaswaq nrradlvyqq  411 24 83720431 YP_441918 36 1 ttacttcgcc ggaacggtcg tcttctgcac ttcctgcgtg ccgaccactt cgacttccac  61 gcggcggtcc ggtgcgaggc aagcgatgag ctgcttgcgg ttcttctgct tgcaggtgtt  121 gcccgtgatc gggttgcgct tgcccttgcc ttccgtgtag accttgttcg ccgggacgcc  181 cttgctgacg aggtacgact tgacggcttg cgcacggcgc agcgacagac ggtcgttgta  241 cttgtccgag ccgatgcggt cggtgtagcc cgtggccacg accacttcga cgttcatgcc  301 ttggatcttc gcggccagtt cgtcaagctt ctgcttgccg gccggcttca ggatggcctt  361 gtcgaagtcg aacagcgtat cggcttggta cgtaatcttc tggctcgtga tggccggagc  421 aaccggtgcg accggtgccg gggccggtgc ttgggcgacc agcgcgccgt cgcacttcgc  481 gttggcggtg gccggcgtcc agaacgcatc gcgccagcag agctcgttcg tgccgttcat  541 ccacacccat tcgcccgtgc cattcaccca gttgtcattc acggcttgtc gcgacgccgg  601 caccgactgt gccgaagcgg atgcagccat aactgcggta gctgcaatga acgcgagctt  661 tgaaagttta ttcat  11 1 mnklsklafi aatavmaasa saqsvpasrq avndnwvngt gewvwmngtn elcwrdafwt  61 patanakcdg alvaqapapa pvapvapait sqkityqadt lfdfdkailk pagkqkldel  121 aakiqgmnve vvvatgytdr igsdkyndrl slrraqavks ylvskgvpan kvytegkgkr  181 npitgntckq knrkqliacl apdrrvevev vgtqevqktt vpak  412 40 83719322 YP_442131 37 1 ttagaagcga tgacgcaggc ctgccgtgac ggcgatctgc ttctcgctcg acgacatgcc  61 gccgacgccg ttgatatagg cgccgaggcc cgtgccgtcg ctgttgacct tctgatacac  121 gccttgcagg tacacgtccg tgcgcttcga cagcgcgtag gccgtttgca ggttgaactg  181 attccagccc gggtggtggt tctcgatgaa gccggcggtg tacgtgtacg agcccgcgac  241 cgtccatgcc ggcgtgatcg cgtagcgcgc gttcacttcg tagttgttga aacgcatgaa  301 cgtgccgtcg agcgcgatgc cgctcgacac gcccgatgcg cccgagctga tcgccgtcgc  361 gcgattgacg cgcgattgcg tgaacacgaa gccggccgtc gccgggccgt acgtgtaatt  421 caggccgccg ccgaacacgc gctggcgctt gccgacgaac gtattgtcga gcgcgaccgc  481 gcccgaagcg ttcgacacgg tcgggccgaa gtcgttgttc aactgcaggt agccggcgcc  541 gacgttgaag ccggcgtagc tatacgacac gcccgcgcta tacgcacggt tgttcgcgaa  601 ctcgttgctg ttcgagaagc cgtacaacgc gccgaacttc aggccggccc agttcacgct  661 cgtgtacttg accgcgttgt tgatccggaa cgagttgttc aggttgtcgt tgtcgaacgg  721 gtgggcaaac tgcacgccgc cgaattgcgt gccggtcagc gacagcggcc cgatgtagtc  781 gacgacgcta tcgtactgac ggccgagcgt cagggagccg tattgatcgt gcgacagacc  841 gacgaacgcc tggcggccga actcgcggcc gttctgcttg agcgtgccgt tattgatgcc  901 gaagccgttt tccaacacaa aaatcgcctt caggccgccg ccgaggtctt cggcgccgcg  961 caggccccag cggctgccgt tgaccgagcc gctggactgc gaccatgcac tgtggccgcc  1021 ttggttgttc gtgtaggtga tgccggcgtc gatgaggccg tacagcgtca cgctgctttg  1081 cgcgtgagcg gccgtcgcga agacgccaga gagagcagcg acgatgaggg tctttttcat  12 1 mkktlivaal sgvfataaha qssvtlygli dagitytnnq gghsawsqss gsvngsrwgl  61 rgaedlgggl kaifvlengf ginngtlkqn grefgrqafv glshdqygsl tlgrqydsvv  121 dyigplsltg tqfggvqfah pfdndnlnns frinnavkyt svnwaglkfg alygfsnsne  181 fannraysag vsysyagfnv gagylq1nnd fgptvsnasg avaldntfvg krqrvfgggl  241 nytygpatag fvftqsrvnr ataissgasg vssgialdgt fmrfnnyevn aryaitpawt  301 vagsytytag fienhhpgwn qfnlqtayal skrtdvylqg vyqkvnsdgt glgayingvg  361 gmsssekqia vtaglrhrf  413 44 83716488 YP_439650 38 1 tcagaacttg tgacggatcg ccgcgcggac cgcgaactgg ttcgccgatg ccgacgggcc  61 gtccgtgccg acgacgtagc cgccatccgc gatcgtgccc gtcttgtcgc ccgcaacctt  121 ctggtatgcg ccctgcagat agacgtcggt gcgcttcgac aggttgtagt ccgccatcag  181 gccgatcgtg tggtacttcg gcttgaccgt gcccgcagcc gcgtcgaact tgccgtccgt  241 gtacacatac tgcgcgccga tgaagagcgc cggcgtcagt tggtacttgc cgttgatttc  301 gaagttctgg aacttcgtcg cggtcagacc gaggcccgcg aacgtcgccg tcggcagata  361 gaccgtcgag accgggttct tcacgtcggt cttcgtgtag acgaagccga ccgtcgccgg  421 gccgaacgtg tagttgacgc cgccgccgaa gatgcgcagg cggtcggccg tgaagttcgc  481 gtcgttgtcg gcgatcgcgc cgcccgccgt cttgcccggg ttgttcgcct gcaggtacgc  541 ggccgcgagt tgcaggccgc ccagcgtgta cgcggcgccg atgctgtatt gacggttgtt  601 cgagaagccc gtcgcgttgc tgaagctgta cgtgccgccg acttgcagac cgttccagtc  661 cgggctcgcg tacttgatcg tgttgttcac acggaacgag ttatccgtgt tgtcgttgtc  721 gaacgggtgc gagaacagca ggccgcccca gttgccgttc gccgtcagcg gcgcgaggta  781 gtcgacgacc gaatcgtact ggcggccgag cgtgagcgaa ccgtattgcg cgtgcgacag  841 gccgacgaac gcctggcggc cgaacatccg gccgccctgg ccaagccggc cgttattcac  901 gtcgaagcca ttttccaacg tgaagacggc cttcaggccg ccgcccaggt cttccgagcc  961 gcgcaggccc cagcggctgc cctgcgcgaa gccgctcgcg agctggtagt tgctcttgcc  1021 gacgccgttg acgttcacgt tgctcgtgta gttgaaaccc tcgtcgatca cgccgtacag  1081 cgtgacgctg ctttgggcga aaaccggcgc ggcgaatgcg agcaatgcgg cagcggaaat  1141 gatgtgcttt ttcattatcg atatctccag accttgattc ggcggcctcg cggatgtcgg  1201 gccgccacga tgtgttcggc gtttctgcgc cccattccgg ggcat  13 1 mprngaqkrr thrggptsar ppnqgleisi mkkhiisaaa llafaapvfa qssvtlygvi  61 degfnytsnv nvngvgksny qlasgfaqgs rwglrgsedl ggglkavftl engfdvnngr  121 lgqggrmfgr qafvglshaq ygsltlgrqy dsvvdylapl tangnwggll fshpfdndnt  181 dnsfrvnnti kyaspdwngl qvggtysfsn atgfsnnrqy sigaaytlgg lqlaaaylqa  241 nnpgktagga iadndanfta drlrifgggv nytfgpatvg fvytktdvkn pvstvylpta  301 tfaglgltat kfqnfeingk yqltpalfig aqyvytdgkf daaagtvkpk yhtiglmady  361 nlskrtdvyl qgayqkvagd ktgtiadggy vvgtdgpsas anqfavraai rhkf  414 39 83718334 YP_439716 39 1 ttagaagcgg tgacgcagac caaccgtcgc tgcggtttgg ttgatcgacg tgctgaacgg  61 cgtgctgagg tcgccgttgt agatcgacgc attcgcgttc ttcgacgaac gctggtacac  121 agcttgcgcg tacacgtcgg tgcgcttcga cagcgcgtag tcggcttgga cgccaacttg  181 gttccagtgc gcgctttcgc cgttcgcctt cgcgttcgtg tacgtgtaag caacgcccag  241 acccagtgcc ggcgtcaggt tgtacttcac gttcgcttcg tagttgtcgg cacggatggt  301 cggcgcgccg tttgccaggt tgtcgagacg cgattgcgtc cagagcaggc cgccttgcag  361 cgggccgtaa gcgtagcttg cgccagcgcc gtacacgcgc gaacggcctt ggatgcttgc  421 tgcgttcgtg ccgccgatgt tgaagccggt cagcggatcc gttgcgcccg tggtgttcgc  481 gcctgcgttg ttagcttgcg agtacgcggc accgaccttc aggccttgga actggtacga  541 agcgcccgcg ctgtacgcac ggttgttcgc gaattgcgag ttgttcgaga acgagtacgt  601 gccgccgaat tgcaggccgg cgtagttcgc gctcgtgaac ttgaccgtgt tgttcaccgc  661 gacgtcgccg ttcgtgttca ggcggtcgtt gttgagcggg tgcgcgaagt acgtgccacc  721 ccaggtgccc gttgccgaca gcggcgacag gtagtcttgg gtcgcgtcgt actgacggcc  781 cagcgtgacg gtgccgtagt tgctcgacag gccgacgaac gcttgacggt tgaacatgcc  841 gccaccgttg ttgaagcggc cgttaccgat gttgaagccg ctttccaacg tgaagatcgc  901 cttcaggccg ccgcccaggt cttccgaacc gcgcaaaccg aaacggcttt ggtcgacgcc  961 ggcgccgacc gaccacagcg acttacccga gcccgacggc gtcgcgacgt tgctttggta  1021 cgtgatgccc gcgtcgagca cgccgtacag cgtgacgctg ctttgcgcgt gtgcgacggt  1081 agcgaacgat gcagcaactg ctgcaacaat cagagtcttg ttcat  14 1 mnktlivaav aasfatvaha qssvtlygvl dagityqsnv atpsgsgksl wsvgagvdqs  61 rfglrgsedl ggglkaiftl esgfnigngr fnngggmfnr qafvglssny gtvtlgrqyd  121 atqdylspls atgtwggtyf ahplnndrin tngdvavnnt vkftsanyag lqfggtysfs  181 nnsqfannra ysagasyqfq glkvgaaysq annaganttg atdpltgfni ggtnaasiqg  241 rsrvygagas yaygplqggl lwtqsrldnl angaptirad nyeanykynl tpalglgvay  301 tytnakange sahwnqvgvq adyalskrtd vyaqavyqrs sknanasiyn gdlstpfsts  361 inqtaatvgl rhrf  415 55 83716254 YP_438901 40 1 ttagaacttg tggcgcaggc ccagggcaac catctcttgc gacttcgtac cgccgatgcc  61 gtacgagccg atcgacgcct gtgcgtcgcc gccgcccgct tgcgtgccgc ttgcgtgctg  121 gtacgcgcca gccagataga cgtcagtccg cttcgacagc gagtagtctg cgcccagcga  181 aacctggtgg tacttcgcgt tcgaatcgcc gctcgacttc gtgtagatat aaccgaggcc  241 cagcagcagc gccgacgtcg cctggtacgt gacgaagccg cggcccgtgt tgtacttctc  301 ggtcgagcca aatgccgatg cgctgtccga cttgtattgc gcgttgctgt agccgaggcc  361 gaacgtgaac gggccaaaca catattgagc gccgacttga gcgatgccga tcgacttggc  421 cgtcgcgtag ccgttgttca tgaacagcga gttgtccggg ccatcgaaga tcgcgtcgga  481 ggagccgccc cagcccgtgc gaacgcctgc tgcgctaagc ggagccgagt tcgatgcgtg  541 gaagtagccg gccccgatgc cgaccgggcc gttgttgtac gccgcggcgg ccgcccacgt  601 ctgtccggta cccggcgagc ctgcgatgcc gctgaagccg tacaacgcct cgaactggaa  661 gccggcaaac acgggcgacg tgtacttgac cgtattgttc acgcgcaggc tgttgtcgtt  721 gttgtcgacg tcacccgggg tggcgaacag gctgccgaag tagttgtctg cagtgacggg  781 ctgaaccagg tcgacgagcg gatcgtattg acggccgagc gtcagcgtgc cgtacgtatc  841 gctttgcacg ccgacgaacg cctgacggcc gaacatgcgg ctgccttggc cgagcgcgcc  901 gttagccgaa ttgaagccgt tctcgagctg gaaaatggct ttcaggccgc cgccgagatc  961 ctcggtgccc ttcaggcccc agcgggcacc ttgcaggttg ccgctgccca tttgccatgc  1021 gttgttggcc ttgccgtcgt taccatggac ataagtgatc gacgtatcaa tcacgccata  1081 cagcgtgacg ctgctttgag cctgagctgc accggccgcg cccagcagcg cgagcgagag  1141 ggtagacaga gcgagtttct tcattgagct atttctccac gcgagtgagt cgttatttgt  1201 tgcggattgg agaatagcgc actgcatcaa atgcaagaaa tccaaaaaaa taaagtgtct  1261 caaaaaggtg acagcgatga aaagtctttt tatatcaatc acataaacga ccatttcgat  1321 tccgggaaca ctggccgatg gttgcgttgc gattgttgcg gaagcaagca tattgcttgt  1381 gtgtggggcc gttttcgggc tgttttcgca gcaaagtcgg tgcatgcaaa cgtttgcggg  1441 cgtggcggtg gctgcgatgg cgacaggcgc gcgctcgaac gccgcactct gctccgacac  1501 gacgccggca tctttccgcg ccaggtgcgc ggtagatcga gcaggacgtt tgctcgggcg  1561 caatacgcgc aa  15 1 mrvlrpskrp arstahlark dagvvseqsa aferapvaia atatpanvcm hrlccenspk  61 taphtsnmla satiatqpsa svpgiemvvy vidikrlfia vtflrhfifl dflhlmqcai  121 lqsatnndsl awrnssmkkl alstlslall gaagaaqaqs svtlygvidt sityvhgndg  181 kannawqmgs gnlqgarwgl kgtedlgggl kaifqlengf nsangalgqg srmfgrqafv  241 gvqsdtygtl tlgrqydplv dlvqpvtadn yfgslfatpg dvdnndnslr vnntvkytsp  301 vfagfqfeal ygfsgiagsp gtgqtwaaaa aynngpvgig agyfhasnsa plsaagvrtg  361 wggssdaifd gpdnslfmnn gyataksigi aqvgaqyvfg pftfglgysn aqyksdsasa  421 fgstekyntg rgfvtyqats alllglgyiy tkssgdsnak yhqvslgady slskrtdvyl  481 agayqhasgt qagggdaqas igsygiggtk sqemvalglr hkf  416 58 83720933 YP_442964 41 1 tcacgcacac gcgtcttcgc cgccgcacgc ggcgcgcgac cagccgccgc cgagcgcgcg  61 atacagcgtg atggcgttcg tgagccgcaa ctgcttcagg cgaatcagtt cctgcccgga  121 ctcgaacgtg ctgcgctgcg cgtcgagcaa ttcgagatag cttgcgacgc cgctgccgta  181 gcgccgctcc gcgagccgca gccgctcggc gtccgcgccg tagaccgcct gctgcgcggc  241 gagctgcgca tcgatctgat cgcgcgcggc gagcgcgtcg gccacttcgc ggaacgccgt  301 ctggatcgtc ttttcgtatt cggcgaccgc gatatgcttg cgcgcgtcgg cgacatcgag  361 attcgcgcga ttgcgtccgc ccgcgaagat cggcagcgtg agccgcggcg cgaacgtcca  421 cacgctcgag cctgccgaga agagccccga gaacgcgtcg ctcaccgagc cgatgtcggt  481 cgtgagcgcg atgcgcggga agaacgccgc gcgcgccgcg ccgatgttcg cgttcgccgc  541 gacgagccgc tgctcggcct gccggatgtc aggccgctgc tcgagcaggt ccgacgacag  601 ccccggcgac acccgcgcga ccgcgagttc gtcgagcgcg ggcgcatcgg cgggcagggc  661 cgccgtgaag tcgcccgcga gcagcttcag cgcgctcgcc gcctgcgtgt gctcgcgctc  721 gagcgccgcc ttcgatgcgc gcgcggacgc gacgagcatc tcggccgtgc gcagctcgat  781 cgccgtgctc gtgcccgccg cgtagcggcg ctgcgtgagc gcgtaagtcg cgtcgcgcgc  841 ggcgagcgtg cgttcggcga gcttcagttg gtcgacgagc gcgcgctcgc gcacataggc  901 ggacgcgact tcggcgatca ggctgacgcg cgccgcgcgc tgcgcctcgg cggtcgcgaa  961 gtactcggcg agcgccgcgt ccgacaggct cttcacgcgg ccgaacaggt cgatctcgaa  1021 cgcgctcaag ccgacgctcg cgcgatacag cgagctcgtc gcgctctcgc gcagcaccgg  1081 gtcgtagagc cgcgtgcgct cgtagccgag gctgccgtcg atcgacggca gccggtccgc  1141 gcgcgcgacg ccgtaaaggc cgcgtgcctc ctcgatgcgc agcgtcgcga tccgcagatc  1201 gcgattgttc gcgagcgccg cgtcgatcag cgcgcgcagc gccggatcgg tgaagtacgc  1261 gcgccagtcg tcgaggcgca catcgtgcat ctcctgcaca ttgccgccgc cgcgcgcgtc  1321 ggacgctggc tgcgcgtcgc gcgcgccgct gccggctgcc gccgggctcg cggcgcgcgg  1381 cgcgcccgcc gccgcgggca gataattcgc cggcacgggc gcggccggac gctcgtagcg  1441 cggcgcgagc gtgcagcccg tgacgagcgc ggccgcgagg gcggccggcc atgcccgcag  1501 cccgcccggc cgccgcgaac cgtgcggccc gccctgacgg gccaggggtt tcatcatcgt  1561 tttcacttga tctccagtgc cgcgcgcgcg ccgccgcggc gacgcggaac gatgtcgacc  1621 acgcggccga cgcat  16 1 mrrprgrhrs asprrrargt gdqvktmmkp larqggphgs rrpgglrawp aalaaalvtg  61 ctlapryerp aapvpanylp aaagapraas paaagsgard aqpasdargg gnvqemhdvr  121 lddwrayftd palralidaa lannrdlria tlrieeargl ygvaradrlp sidgslgyer  181 trlydpvlre satsslyras vglsafeidl fgrvkslsda alaeyfatae aqraarvsli  241 aevasayvre ralvdqlkla ertlaardat yaltqrryaa gtstaielrt aemlvasara  301 skaalereht qaasalklla gdftaalpad apaldelava rvspglssdl leqrpdirqa  361 eqrlvaanan igaaraaffp rialttdigs vsdafsglfs agssvwtfap rltlpifagg  421 rnranldvad arkhiavaey ektiqtafre vadalaardq idaqlaaqqa vygadaerlr  481 laerrygsgv asylelldaq rstfesgqel irlkqlrltn aitlyralgg gwsraacgge  541 daca  417 55 83718989 YP_441238 42 1 atgaagcgaa aacatgcttt gactgcactc gcagtcgcgc tgctcgccgc gggctgcacg  61 ctcgcgccgc gctacgagcg tccggccgcg cccgtgtcgg gcgcgttccc gaccgacggc  121 gtctacgccg cgcagccggg cgccgcggcc ggcgcgcgca gcgcgaacgg ccaggcggcc  181 gtcgacatcg gctggcgcga gttcttcgtc gatccgcgcc tgcagcggct gatcgagatc  241 gcgctgaaga acaaccgcga tctgcgcgtg tcggtgctca acgtcgaggc gtcgcgcgcg  301 cagtatcgga tcacgcgcgc gggcctgttc ccgacgctcg acggcaccgg cacgggcacg  361 atccagcgca cgccggccgg tgtgtcggta accggccagc cgctcatctc gcggacctac  421 aatgtcggcg tgtccgcgtc gtgggagctc gacctgttcg gccgcgtgca gagcctgaag  481 gatcaggcgc tcgcgcaata cttcgcgacc gcgcaggcgc gcaaggccgc ggagatctcg  541 ctcgtcgcga gcgtcgccga tcagtatctg acgctgctgt cgaccgacga tctgctgcag  601 atcacggaga acacgctgaa gacggcgcgc gcgtcgtacg acctgacgaa gctgcagttc  661 gacaacggca ccggctcgga gctcgatctg cggcaggcgc agacggtcgt cgagaccgcg  721 ctcgcgaacc agcaggcgca ggcgcgcgcc cgcgcgcagg cggtcaacgc gctcgtgctg  781 ctgatcggcg agccgctgcc cgacgatctg ccggccggcc tgccgctcaa tgcgcagaat  841 ctgctgaccg acatcccggc cgggctgccg tccgatctgc tgacgcgccg ccccgacatc  901 atgcaggccg aggagacgct gcgcgcggcg aacgcgaaca tcggcgcggc gcgtgcggcg  961 ttcttcccga aaatctcgct caccggcgcg ttcggcaccg cgagcccgac gctcggcggc  1021 ctgttcaagg cgggcacggc ggcgtggtcg ttcgcgccga gcatcgcgct gccgatcttc  1081 gagggcggac agaacatcgc caatctcgat ctcgcgcacg tgcagaagcg catcgagatc  1141 gcgaactacg agaaggcgat ccagagcgcg ttccgcgagg tgtcggacgg gcttgccgcg  1201 cgcggcacgt acgatcagca gatcgcggcg ctcgagcgca acgagcacgc gcagcagcgc  1261 cgctacgatc tgtcggacct gcgctacaag aacggcgtcg acagttatct gtcggtgctg  1321 accgcgcaga cggatctgta ttcggcgcag cagcaactga tcagcgcgcg gctcgcgcgc  1381 tggacgaacc tcgtcgatct gtatcgcgcg ctgggcggcg gctggatcga acgcgcgggc  1441 gagacgccgc gcccggcgga tgcgcctgtc gactacggca aggcggcagc gcctgcgccg  1501 gcgtcggcgt cggcgtcggc ggcgcccgct gcggcggcca gcgcgccggc ggccggctga  17 1 mkrkhaltal avallaagct lapryerpaa pvsgafptdg vyaaqpgaaa garsangqaa  61 vdigwreffv dprlqrliei alknnrdlrv svlnveasra qyritraglf ptldgtgtgt  121 iqrtpagvsv tgqplisrty nvgvsaswel dlfgrvqslk dqalaqyfat aqarkaaeis  181 lvasvadqyl tllstddllq itentlktar asydltklqf dngtgseldl rqaqtvveta  241 lanqqaqara raqavnalvl ligeplpddl paglpinaqn lltdipaglp sdlltrrpdi  301 mqaeetlraa nanigaaraa ffpkisltga fgtasptlgg lfkagtaaws fapsialpif  361 eggqnianld lahvqkriei anyekaiqsa frevsdglaa rgtydqqiaa lernehaqqr  421 rydlsdlryk ngvdsylsvl taqtdlysaq qqlisarlar wtnlvdlyra lgggwierag  481 etprpadapv dygkaaapap asasasaapa aaasapaag  418 19 83717287 YP_440214 43 1 gtggcaagcg aagatcggca agattccggt cgcgagcgcg taaataggaa tgattttcag  61 gccggatttc aatccggcct ttttgatgcg gcgaacccgt tccacctttc ggatatcgaa  121 cggattcgcc cttggctttt cgcgaacgag cgtttatcgt tcaggaactc aagcccccac  181 acgtcacaag gaacgaacat gcaaggcgac aagaaagtca tcgaatatct gaacgcgcag  241 ttgaaaaacg agctgaccgc gatcaatcaa tatttcctgc atgcgcggat gtacaagcac  301 tggggtctcg agaaactcgg caagcacgaa tacgacgaat cgatcggcga aatgaagcat  361 gcggactggc tgatcgaacg cgtgttcatg ctcgacggcc tgccgaacct gcaggatctg  421 cacaagctgc tcatcggcga ggaaaccgag gaaatcctga aatgcgatct gaagctcgaa  481 caggtctcgc aggcgacgtg caaggaagcc atcgcgtatt gcgaatcggt gcgtgattac  541 gtgtcgcgcg aaatcttcga gaaaatcctc gacgacaccg aagagcatat cgactggctc  601 gaaacgcaaa tcgacctgat cggcaaggtc ggcattcaga actatcagca gtcgatgatg  661 ggttcgccgg agtaa  18 1 masedrqdsg rervnrndfq agfqsglfda anpfhlsdie rirpwlfane rlsfrnssph  61 tsqgtnmqgd kkvieylnaq lkneltainq yflharmykh wgleklgkhe ydesigemkh  121 adwliervfm ldglpnlqdl hklligeete eilkcdlkle qvsqatckea iaycesvrdy  181 vsreifekil ddteehidwl etqidligkv giqnyqqsmm gspe  419 8.5 83718020 YP_440216 44 1 atgatcgtct gcgtgtgcaa gtccgtttcc gatcggaaga ttcgcgcatc cctcgcggaa  61 ggcgtgaaca ccttcgaaga actccagttc gaactcgggg tcgccacctg ctgcggcaag  121 tgcgaggaaa ccgtgcgcga gatcatggcg gaacagggcg tttgtgcgag ccgctgcggt  181 gtcgagcccc ccgcggccgt gccggtcccc gtcacgttct acgaacgcaa ggcggcctga  19 1 mivcvcksvs drkiraslae gvntfeelqf elgvatccgk ceetvreima eqgvcasrcg  61 veppaavpvp vtfyerkaa  420 36 83718178 YP_439311 45 1 gtggggcgca tcgggtaccg gcggcgcgcg gctcgttcgc gcgccgcatc gagaggcaag  61 cgcatgctga atttcctggt caaacgactg ttcgggctgc tgccgacgct cgcgtgcgtc  121 gcggtgctcg tgttcctgtt cgtccacctg ctgccgggcg atcccgcgcg gctcgcggcc  181 gggcccgaag ccgacgacgc gacggtcgcg ctcgtgcgcg ccgatctcgg cctcgacaag  241 ccgctgcccg cgcagttcgc gagcttcttc gcgcggatcg cgcaaggcga cttcggcgtc  301 tcgacgcgca gcaagcggcc cgtcgcgacc gagatcggcg agcgcttcat gccgacgctg  361 tcgctgaccg tcgtcagcat ggcctgggcg acgctcttcg ggatggcgat cggcatcgcg  421 tcggccgtgt ggcgcaaccg ctggccggac cgcgtcggca tgacgctcgc ggtgtcgggc  481 atctcgtttc cggcgttcgc gctcggcatg ctgctgatgg aagtgttctc ggtgaagctc  541 ggctggctgc ccgtcgtgcc ggacggctcg tggaagagct acgtgctgcc gtcgctgacg  601 ctcggcgcgg ccgtggccgc cgtgatggcg cgcttcacgc gcgcttcgtt catcgaggtg  661 ctgaacgagg atttcgtgcg caccgcgcgc gcgaagggcg tgcgcgagcc gatggtcgtg  721 ctcaagcact gcctgcgcaa cgcgatgatt ccggtcgtca cgatgatggg gctgcagttc  781 ggcttcctgc tcggcggctc gatcgtcgtc gaggcggtgt tcaactggcc ggggctcggc  841 cgcctgctcg tcgatgcggt gacgatgcgc gactacccgg tgatccaggc gatcgtgctg  901 ctgttctcgc tcgaattcat cctgatcaat ctgaccgtcg acgtgctgta cgcggtcatc  961 aacccgacga tccgtttcaa gtga  20 1 mgrigyrrra arsraasrgk rmlnflvkrl fgllptlacv avlvflfvhl lpgdparlaa  61 gpeaddatva lvradlgldk plpaqfasff ariaqgdfgv strskrpvat eigerfmptl  121 sltvvsmawa tlfgmaigia savwrnrwpd rvgmtlavsg isfpafalgm llmevfsvkl  181 gwlpvvpdgs wksyvlpslt lgaavaavma rftrasfiev lnedfvrtar akgvrepmvv  241 lkhclrnami pvvtmmglqf gfllggsivv eavfnwpglg rllvdavtmr dypviqaivl  301 lfslefilin ltvdvlyavi nptirfk  421 42 83718226 YP_439914 46 1 tcagaagcga tggatcagac cgacgccgac gcccacctgg ctgcgcgacg acgacggcga  61 cgtgttgaag ccgtcgccga tcgacgcggt cgcgtcgatg atcttgccgc ccgcgagcgt  121 cttgccgttc gcacgctggt acgcctcgac cgcgtaaagg cccgtgcgct tcgacaggct  181 gtaatactgc gacagcgtga actggtggta ctgcgccgcg ctcgtgatgc cgttcgattg  241 cgtcgcgcgc gtgtacgcgt agccgcccgc gaagtcccac tgagcggacg gcttgaagtg  301 cagcaccgca cccgccgtgt tgaagatcgc ggtgttgcgg aacgccgagt tgacgcccgg  361 aatgtactgc acgttcgagt acgacaccga gatgtcccac gcggacgaga actggtagcc  421 ggccgtgacc gcgacgcgct gctgcgcctg cgcggtcttg tagccgttgt tgatcgccga  481 cacggccgtc tgcgcgccgc cgttggacgt cgtcgaattc gcgccccagt cgccgccgcc  541 cgacgtcgag ttgttcacgc gctggaacgc cgccgcgatg ccgagcggac cgttcatgta  601 ctggatgccc gcgctccacg tggagccggc gttcacgctg cccggctggc cgccgaacgc  661 atacgagccg ccgaacgtga agccgtagaa cttcggcgac atgtagacga gcgagttgtt  721 cgtgcggtag ctggtgtcga gcgagtcgat gtcgcccggg tgcgcgccga agtaaccggt  781 gagccaggtc gtcgggctgt acggcgacag cagcgtgtag tacgcggtgt attgacggcc  841 ggccgtcagc gtaccgtacg tcggattggt caggccgacc cacgcctgcc gcgtgaagat  901 gccgcccgcg aactgcgacg tgccgttggc cgtgctgaaa cccgattcga gctggaagat  961 cgccttcgag ccgccgccga ggtcctcgct gcccttcagg ccgaagcggc tgcccgccca  1021 cacgcccgtc gacatcttca ccgccgagcg gccgcccgtc gttgagccga gcgacgtgct  1081 gctgctctga tacgcgatgc cgttatcgac gataccgtaa agcgtcacgc tgctctgagc  1141 gtgcgcggtg gcgacagccg ccaatccggc ggccgtcatc gcgaccgctg tgcgcttttt  1201 at  21 1 mkkrtavamt aaglaavata haqssvtlyg ivdngiayqs sstslgsttg grsavkmstg  61 vwagsrfglk gsedlgggsk aifqlesgfs tangtsqfag giftrqawvg ltnptygtlt  121 agrqytayyt llspyspttw ltgyfgahpg didsldtsyr tnnslvymsp kfygftfggs  181 yafggqpgsv nagstwsagi qymngplgia aafqrvnnst sgggdwgans ttsnggaqta  241 vsainngykt aqaqqrvavt agyqfssawd isvsysnvqy ipgvnsafrn taifntagav  301 lhfkpsaqwd faggyaytra tqsngitsaa qyhqftlsqy yslskrtgly aveayqrang  361 ktlaggkiid atasigdgfn tspsssrsqv gvgvglihrf  422 56 83719074 YP_443076 47 1 ttgccacaac aagtaattca gacagataaa tgtaaggaaa tccttgcagt gtccattatt  61 cattccgagt caggaaacac agcgatgaaa accttcccgt tgtccgcttg ccgaaccgcc  121 acggccgtcg cggtcgccgt gctcgcgctc gcgggatgcg cgaactattt cggcatcaag  181 agcgacaagg cgatcgcgcc cgcgacgcag ttcgagagcg cgcagagcct gccggcgcaa  241 ggcggccggt ggccgtcgct cgactgggcg aaccagttcg gcgatccgca actgcccaag  301 ctgatcgacg aggcgctcga aggcaatccg acgatcgcgc aagcgcaggc gcgcatcgcg  361 aaggcatcgt cgtacatcga atcgtcgcgc tcgacgctgc tgccgaaggc ggacgcgaag  421 tactcgtgga cccgcgagct gtattcgagc aacgccctcg tcccgccgcc cttcggcggt  481 cagtggtaca gcgagaacaa cgcgctcgcg agcgcgtcgt gggaactcga cctgtggggc  541 aagaaccgcg cgcgcctcaa tgcggccgtg tcgcaggaaa aggccgccga agccgacatg  601 cagcaggcgc gcgtgacgct cgcgacgtcg gtcgcgcgca cgtacaacca gctcgcgcag  661 ttgtacgcgc tgcgcgacat cgccgagcgc gagatctcga accggcagac ggtcggcaag  721 atcaccgacg gccgcgtcgg cgcgggcctc gacacgaacg tcgagcgcca gaccgcgctc  781 ggcaacatcg cgacgagcca gtcgacgctg tccgatctcg acggccagat cacgaacgtc  841 cgctatcagc tcgccgcgct gctcggcaag ggcccggacc gcgggctgca gattgccgcg  901 ccggtgctga gcccgggcgg cggggtcgcg ctgccggaca acctgcccgc cgatctcgta  961 tcgcgccgcc cggacctcgt cgccgcgcgc tggcaagtcg aggcggcgat gcacgacgtg  1021 aaggaagcga aggccgagtt cttcccggac atcaatcttg cggcgggcct cggcttcgac  1081 gcgttcggct ggggccgctt cctgaacttc gcgagccgcc aggcgcaatt cggcccggcg  1141 atccatctgc cgatcttcga cggcggcgcg ctgcgcgcgc aactgaaggg ccgctacgcg  1201 gacttcgatc tgtcggtcgc gaactacaac cagacgctcg tgagcgcgct gaacgacgtc  1261 gcgacgcaag tcgcgtcgat ccgctcgatc gacacgcaga tgggcgacgc gcagcgcgcg  1321 ctcgatgcgt cgacgcgcgc gtacgagctc gcggtgatcc gctacaaggc gggcctgtcg  1381 ccgcagctgc aggtgctgaa cgcggacagc aaccggctcg ccgccgagca gacggtgacg  1441 aacctgaaga tgcgccgccg cgatctgcag atcggtctcg tgaaggcgct cggcggcggc  1501 ttcgacgcga ccggcacgcg gctcgccacg cccgcaccgg cagtggcggc ggccgccgca  1561 cccgcgcggc acgcgtcgaa ctga  22 1 mpqqviqtdk ckeilaysii hsesgntamk tfplsacrta tavavavlal agcanyfgik  61 sdkaiapatq fesaqslpaq ggrwpsldwa nqfgdpqlpk lidealegnp tiaqaqaria  121 kassyiessr stllpkadak yswtrelyss nalvpppfgg qwysennala sasweldlwg  181 knrarlnaav sqekaaeadm qqarvtlats vartynqlaq lyalrdiaer eisnrqtvgk  241 itdgrvgagl dtnverqtal gniatsqstl sdldgqitnv ryqlaallgk gpdrglqiaa  301 pvlspgggva lpdnlpadlv srrpdlvaar wqveaamhdv keakaeffpd inlaaglgfd  361 afgwgrflnf asrqaqfgpa ihlpifdgga lraqlkgrya dfdlsvanyn qtlvsalndv  421 atqvasirsi dtqmgdaqra ldastrayel avirykagls pqlqvinads nrlaaeqtvt  481 nlkmrrrdlq iglvkalggg fdatgtrlat papavaaaaa parhasn  423 85 83718695 YP_442137 48 1 atgtcccatg aacttgccgc gcacgtcgcg cgcacgcggc tcgcggccgc ctgcgtcgcg  61 gcgttcgcct ggcccgccgc gcacgccgtc acgacgggcg ccgccgtccc tgccgattca  121 acgtccgccg ccgctgccga gacgaccgca tccgggaaaa ccctggatat cgtcagggtg  181 accgcgcagc gccccgcatt cgcgtccgac acgcccggcg tcgtcgaggc gctcacgcgc  241 gagcagatcg attcgcacgt caacgtgacg accgaagacg cgctcaagta cgcgccgaac  301 ctgatggtgc gccggcgcta tatcggcgat cgcaactccg tgttcgccgg ccgcgatttc  361 aacgagttgc agagcgcgcg cggactcgtt tacgcggacg gcatcctcct gtcgaatctg  421 ctcggctcca gttactcgta tccgccgcgt tggtcgctga tccagcccga cgacatcgcg  481 cgcgtcgacg tgctgtacgg cccgttctcc gcgctctacc cgggcaatgc gatcggctcg  541 accgtgcaga tcacgacgcg caagccggat cggctcgagg cgtcggtgtc gacgcagttc  601 ttcacgcagc gctatcgcga cggctacggc tttgccgaca gcttcggcgg caatcaccag  661 accgcgcgcg tcgccgaccg cgtcgggcgc ttctggtatg cgctgtcgct cgaccggctc  721 gagaacgaca gccagccgat gcaatacgcg agcccgaatg gcacgttcga tccgcggctc  781 ggcgcgagcg tgccggtgac gggcgccgtt tccgacatcg gcccgaacgg ccggcctcgg  841 acgatcgtcg gcgcgcagac gatcgagcgc accgagcagc tcaacgagac gctgcgcttc  901 ggctatgcgt tcaccgacca cgtcgatgcg acggttacgc tcggccactg ggagaatcac  961 taccggcagc acggcgacac gttcctgcgc gacgcggcgg gcaacccggt atacggcggc  1021 aacgtgtcgt tcggcgggcg cagctacacg gtgtcgccga ccgcgttcgc gccgcagacc  1081 ggcgaccagg agaactggct gtacgggctc gggctcgacg cgcgtctcgc atcaggctgg  1141 aagctgtcgg cgaccgcgtc cgcgtacgag gtgtcgcgcg acgtgctgcg cagcgcgtcc  1201 ggcgcgccga ccggcgcgtg ggacggcggc ccgggcacgg tattccatgg cgacggcacc  1261 ggctggcgca ccgtcgattt gcgagcggag tcgcccgacg tgcgcgggca ccgcttctcg  1321 ttcggctatc acttcgacac ctatttcctg cgcaacgcga cctacaacac ggcggactgg  1381 caaaacgccg tgccgacgac gcttgcgaac cgttatcgcg gcaacacgcg cacgcaggcg  1441 ctgtacgcgc aagacgcgtg gcgtttcgcg cccggctggc tcgcgacgct cggcctgcgc  1501 tacgaacgat gggatgcata cggcggccag ctcggcaacg cgaacgcgac gctcggctac  1561 gccggccgtg gcgcgaccgc gctgtcgccg aagctcgcgc tcgaatggca gccaacggac  1621 gcatggcgcc tgcggctgtc gttcgcgacg ggcacgcgct ttccgaccgt ggccgaactg  1681 ttccagggca cgatctcgaa caacgcgatc gtcaacaaca acccgaacct gcaaccggaa  1741 aaggcgatcg actgggactt cacggccgag cgcgacgtcg gcttcggcgt cgtgcgcacg  1801 agcgtgttcc agagcgatct gcgcaattcg atctacagcc agacgacggt cgcgggcgct  1861 tcgacgtaca cgaacatctc gaacgtcgac cgcgtgcggg tgcgcggcgt cgaactcgcg  1921 ttttcagggc aggacgtcgc gatcaagggg ctcgacgttg acgcgaacgt gtccgcgacg  1981 aatgcgcaga cgctcgccga tgcggcgaat ccgaactacg tcggcgcgcg ttggccgcgg  2041 attccacgga tgcgcgcgaa cttgctcgcg tcgtaccgct tcggcgagca ctggatgacg  2101 agcgtcggcg tgcgctattc ggggcggcag tacaacgcgc tcgacaacag cgacgtgaac  2161 ccgaacgtgt acggcggcac cagttcgttt gcggtcgtcg acctcaaggc gcgctaccgg  2221 ttcgatcggc actggctcgc gtcgttcggc atcgacaacg tgaccgatcg ccgctactac  2281 gtgtttcacc cttatccagg ccgcactttt tatggagagt tgaaatggtc gctgtga  23 1 mshelaahva rtrlaaacva afawpaahav ttgaavpads tsaaaaetta sgktldivrv  61 taqrpafasd tpgvvealtr eqidshvnvt tedalkyapn lmvrrryigd rnsvfagrdf  121 nelqsarglv yadgillsnl lgssysyppr wsliqpddia rvdvlygpfs alypgnaigs  181 tvqittrkpd rleasystqf ftqryrdgyg fadsfggnhq tarvadrvgr fwyalsldrl  241 endsqpmqya spngtfdprl gasvpvtgav sdigpngrpr tivgaqtier teqlnetlrf  301 gyaftdhvda tvtlghwenh yrqhgdtflr daagnpvygg nvsfggrsyt vsptafapqt  361 gdqenwlygl gldarlasgw klsatasaye vsrdvlrsas gaptgawdgg pgtvfhgdgt  421 gwrtvdlrae spdvrghrfs fgyhfdtyfl rnatyntadw qnavpttlan ryrgntrtqa  481 lyaqdawrfa pgwlatlglr yerwdayggq lgnanatlgy agrgatalsp klalewqptd  541 awrlrlsfat gtrfptvael fqgtisnnai vnnnpnlqpe kaidwdftae rdvgfgvvrt  601 svfqsdlrns iysqttvaga stytnisnvd rvrvrgvela fsgqdvaikg ldvdanvsat  661 naqtladaan pnyvgarwpr iprmranlla syrfgehwmt svgvrysgrq ynaldnsdvn  721 pnvyggtssf avvdlkaryr fdrhwlasfg idnvtdrryy vfhpypgrtf ygelkwsl  424 78 83717289 YP_438727 49 1 ttgatacgtt atatcgtttc gttgtcggcg cgcgattcgg cccgtgattc cgtccacccc  61 gagaacaccg caccgattac cgcaatggat caccaccgac gtatcgcgcc gccttccgcc  121 cgcaggctgc atcccttgtc gttgctgctc gccgcgagcc ttgcgcacgg cgagacgggc  181 gagccagccg gccgcccgtc gagcgcgccg ccagcgacgg cgctcgcgcc gatcttcgtg  241 accgcgaacc cgctcggcgc atcggcgctc acgtcgccga cggcgtcgct gtccggcgac  301 gcgttgacgc tgcgccgcgc cgattcgctc ggcgatatgc tgaacggctt gcccggcgtg  361 tcgacgacga cctacggtcc gctcgtcggc cgcccgatca tccgcgggat ggacggcgac  421 cgcatccggc ttttgcagaa cggcgtcgcc gcatacgacg catcgtcgct gtcgtacgat  481 cacgcggtgc cgcaggaccc gctgagcgtc gagcgcgtcg agatcgtgcg cgggccggcc  541 gcgctgctgt acggcggcaa tgcggtcggc ggcgtcgtca acacgatcga caaccggatt  601 ccgcgcgaag cggtcacggg cgtcacgggt gcgctcgacg cgagctacgg cggcgcgaac  661 aacgcgcgcg cgggggcggc gctcgtggaa ggcggcaacg gccgcttcgc gttccatctc  721 gacgcgttcg gccgcgagac cgacgagttg cgcatacccg gctatgcgca ctcggcgcgc  781 cagcgcgcgc gcgacggcga ggacgcgagc gagccgtacg gcaagctgcc gaacagcgac  841 ggccgccgtt acggcggcgc ggcgggcggc tcgtacacgt gggccgacgg ctacgtcggc  901 gcgtcgtaca gcggctacga atcgaactac ggctcggtcg ccgaaaccga tgcgcggctg  961 cggatgcggc aggagcgcgt cgcgatcgcg tcggaggcgc gcaatctgcg cgggccgttc  1021 tcgcagctga aattcgactt cggctacacg aactatctgc accgggaaat cgaggacggc  1081 gtgaccggca cgacgtttcg caaccacggc tacgaggcgc gcgtcgaggc gcgccaccgc  1141 aagctcggcc cgttcgaagg cgcgctgggc gtgcaggtcg gccagaacac gttttccgcg  1201 cttggcggcg aggcgctcgc gccgacgacg cgcacgacga gcgtcgcatt gttcggcctc  1261 gaggaatggc aggcgaccga tgcactgaag ctgtccgccg gcgctcggat cgagcacgtg  1321 cggctcgacc cgagcgcgaa cggggacgat aagtttggcg tcgcgcgctc acgcgatttc  1381 aatgcgggca gcgtgtcggc gggcgcgctg taccagctcg cgcccgcgtg gtcggtcgcg  1441 ggcaacgtgt cgtacacgga gcgcgcgccg acgttctacg aactgtacgc gaacggtccg  1501 cacggcgcga cggggcaata cctgatcggc ctgccggatg cgcggaagga gaaggcggtg  1561 tcgaccgatc tcgcgctgcg ctacgcgaac gggccgaacc gcggcagcgt cggcgtgttc  1621 tacagccgct tgcggaacta cctcgccgaa tacgacaccg ggcggctcgt cgacgacgaa  1681 ggcgcgcccg tggccccggg gggcgacgag acgctgcgcg aggccgtcta tcgcggcgtg  1741 cgcgcggagt tctacggcgt cgagctcgag ggcaagtggc gcgcgttcga gaagcgcggg  1801 catcgcatcg atctggagct gggcgccgac tacacgcatg cgcgcaacgc ggacacgggc  1861 gagccgctgc cgcggatcgc gccgctgcgc gcgacgctcg cggccgatta cggctacggt  1921 ccgttcggcg cgcgcgcgca gctcacgcat gcgtgggcgc agcatcgcgt gccggaaaac  1981 gatctggcga ccgacggcta tacgtcgctc ggcgtcgtgc tgacgtacaa gttccgcgtc  2041 ggcgcgacga actggctcgc gtacctgcgc ggcgacaacc tgaccaacca ggatatccgc  2101 tatgcgagct cagtcgtgcg caacatcgcg ccgcagggcg ggcgcagcgt gacggtcggc  2161 atgcgcacga cgttctga  24 1 miryivslsa rdsardsvhp entapitamd hhrriappsa rrlhplslll aaslahgetg  61 epagrpssap patalapifv tanplgasal tsptaslsgd altlrradsl gdmlnglpgv  121 stttygplvg rpiirgmdgd rirllqngva aydasslsyd havpqdplsv erveivrgpa  181 allyggnavg gvvntidnri preavtgvtg aldasyggan naragaalve ggngrfafhl  241 dafgretdel ripgyahsar qrardgedas epygklpnsd grryggaagg sytwadgyvg  301 asysgyesny gsvaetdarl rmrqervaia searnlrgpf sqlkfdfgyt nylhreiedg  361 vtgttfrnhg yearvearhr klgpfegalg vqvgqntfsa lggealaptt rttsvalfgl  421 eewqatdalk lsagariehv rldpsangdd kfgvarsrdf nagsysagal yqlapawsva  481 gnvsyterap tfyelyangp hgatgqylig lpdarkekav stdlalryan gpnrgsvgvf  541 ysrlrnylae ydtgrlvdde gapvapggde tlreavyrgv raefygvele gkwrafekrg  601 hridlelgad ytharnadtg eplpriaplr atlaadygyg pfgaraqlth awaqhrvpen  661 dlatdgytsl gvvltykfrv gatnwlaylr gdnltnqdir yassvvrnia pqggrsvtvg  721 mrttf  425 43 83720836 YP_441236 50 1 atgcgcgtcg aacgggttcc ataccgctta atcactgtcg cgacggctgc cgtttttctg  61 gccgcgtgcg gaaaaaaaga atcggctccg ccccctcaaa cgcccgaagt cggcgtcgtc  121 accgtccagc cgcaagccgt gccggtcgtc tccgaattgc cgggccgcac gagcgcgtat  181 ctggtcgcgc aggtgcgcgc gcgggtggac ggcatcgtgt tgcggcgtga gttcacggaa  241 ggcagcgacg tcaaggccgg ccagcgtctg tacaagatcg atcccgcccc gtacatcgcg  301 cagttgaaca gcgcgaaggc gacgctcgcg aaggcgcagg cgaatctcgc gacgcagaac  361 gcgctcgtcg cgcgctacaa ggtgctcgtc gccgcgaacg cggtcagcaa gcaggactac  421 gacaacgcgg tggccacgca agggcaggcc gccgccgacg tggcggccgg caaggccgcg  481 gtcgacaccg cgcagatcaa cctcggctat acggacgtgg tctcgccgat cacgggccgc  541 gtcggcatct cgcaggtcac gccgggcgcg tacgtgcagg cgagccaggc gacgctgatg  601 tcgaccgtcc agcagctcga tcccgtctac gtcgacctca cgcagtcgag cctcgacggc  661 ctgaagctgc gccaggacat ccagagcggg cgcatcaaga cggagggtcc gggcgcggcg  721 aaggtcacgc tgatcctcga ggacggcaag gcgtactcgg agccgggcaa gctgcagttc  781 agcgacgtca cggtcgacca gacgacgggc tcggtcacga ttcgcgcgat cttccccaac  841 aagcagcgcg tgctgctgcc gggcatgttc gtgcgcgcgc gcatcgaaga gggcgtcaac  901 gacaacgcgt tcctcgtccc gcagatcggc gtcacgcgcg atccgaaggg ccaggcggtc  961 gcgatgatcg tcgacggcaa gggcaaggtc gagccgcgcg tgctcacgac gagcggcacg  1021 cagggccaga actgggtcgt cgaaggcggc ctgcaggcgg gcgaccgcgt gatcgtgcag  1081 ggcatcgaca aggtgcgccc gggcatgacc gtgaagaccg ccgaagctca attgccggcc  1141 gcggcgtccg gcgcgtccgg cgccgcgccc gcgggcggct cgcccgccca ggccgcggcg  1201 gcgagcgcgg ccgcatcggg tgccgcgccg tcgagcgctg ccgccgcgtc gagcgcgaaa  1261 taa  25 1 mrvervpyrl itvataavfl aacgkkesap ppqtpevgvv tvqpqavpvv selpgrtsay  61 lvaqvrarvd givlrrefte gsdvkagqrl ykidpapyia qlnsakatla kaqanlatqn  121 alvarykvlv aanayskqdy dnavatqgqa aadvaagkaa vdtaqinlgy tdvvspitgr  181 vgisqvtpga yvqasqatlm stvqqldpvy vdltqssldg lklrqdiqsg riktegpgaa  241 kvtliledgk aysepgklqf sdvtvdqttg svtiraifpn kqrvllpgmf vrarieegvn  301 dnaflvpqig vtrdpkgqav amivdgkgkv eprvlttsgt qgqnwvvegg lqagdrvivq  361 gidkvrpgmt vktaeaqlpa aasgasgaap aggspaqaaa asaaasgaap ssaaaassak  BpK96243-500 ID MW Protein GI# Protein Locus# SEQ ID NO: Sequence 501 81 53719389 YP_108375 76 1 ttaccagttg tatttcgcgg tcgcgatcac ggtgcgctgg ttgccgtaca tgcacaccgc  61 atccgactgg cagccggcca cgtagcggcg gttgaacagg ttcgtcgcgt tgagcgcgaa  121 acgccagttg cgcagctcgt agtgcagcgc cgcgtcgaac agcgtgtagc tcggcaccgt  181 gagcgaattg tcggccgcgc ccgccgccgc gctcatgtag cgcacgcccg cgccgacgcc  241 gaagcccgtg agcggcccgg tgcgccacgt ccaatcggcc cacagcgacg cgatctggcg  301 cggccgcggc acgtcgacgg gccacttgtt cagcgtgttg tcgttcgccc gcacgttctt  361 cacgtcctga tagacgtacg cggcgatcac cgacagctcg cgcgacaggt tgcccaccgc  421 gctcagctcg acgccgcgcg agcgcacctc gcccacctgc acgaacgtgc tgctcgtcgg  481 gtcgttcgga ttgctcatcg cgacgttcgt ctggttgatc tggtagacgg ccgcgttcag  541 catcaggttc ttgccgggcg gctgccagcg caggcccgcc tcgatctggc ggcccttggt  601 cggcgtcgcg agcccgccgc ccgcgagctt caggccgatc tgcggattga acgacgtcga  661 atagctgaga tacggcgcga gcccgtagtc gccgagatac gtgaggccca cgcgcccgct  721 gaacgcgtgg tcgttctgcc tgaagctcgc cgcgttcgcg atgtcgtcct gcgacgtgcg  781 cgtccagtcc tgccggccgc cgagcgtgag cacccagcgc cgccacttga tctggtcctg  841 cacgtacagg ccgaatgcgt tcagcgtcgt cttcgtgtcg gtgcgcgggt acgcgttcgg  901 cccgctgaag atgtcggacg gaatcggcgt gtagacgggc cggtacaggt tcaggctcgg  961 ccccttcgcg agccattcgc tatcggtcgt cgtctggcgg ttgtagtcga agccgaacag  1021 cagcgtgtgc gaaagcgggc ccgtcgtgaa tttcgcctgc gcctggttgt cgacgtcgaa  1081 gcggctgtag ttgaactgga acaggcccgc gtagcgcgtc atcgtcgcca tcgtcgggtc  1141 tgcgtcgtcg aggccgccgc cgtagacgga cgcgtcgtcg agcgacaggt gcatccagcg  1201 cacgttctgc cgcagcgtcc acaccgggtt cagcttgtgc tcgaactgat agccgagcga  1261 ccactgcttc ttgcggtaat ggtcgaagtt cgcatcggcc gtgtacagat cgtccgagat  1321 cgtgccgttc ggattcggca gcacggtgcc gcgcgacggc aggaagttgc tcgacgtgtc  1381 gccccagtcc tgcaggtacg tcgcggcgag ggtgagcgac gtgtccgcgt tcggctgcca  1441 tttgagcgac ggcgcgaacg acacgcgctg gtcggcgagc ggccccgtct gcgcgttgcc  1501 gtcgcggccg acgccgacga tccggtacga cagcgtgccg tccttgccga tcgtgtcgcc  1561 gatgtcgaac atcagctgct tgcgcgcgta gttgccgacc tgcacgccga gctcgcggat  1621 gcgctcgccg ttcgcgagct tgctctgcac gtcgacgatc gcgccgggat cgccctgccc  1681 gtacagcacc gacgtcgggc cgcgcagcac ggcgatgctg tcgatcatgt acggatcgac  1741 gcgccagctc gacaggttga tcgtgttcgg cacctgcagc ccgtcgacga acacggtcgg  1801 cgtgaagccg cgcagcgccg cgtaccagtc cgaacggttg tccgacccgt agctcgagaa  1861 gcccggaatg tagcggaacg cctgattgat gtcggtcgcg cccgtcgcct cgatctgctg  1921 cgcggtgacg acgttgatcg tctgtggaat ctcgatgatc ggcgtatccg ttttcgtgcc  1981 cgtcatgctg cgccgcgcga cgaggccgac gctcgcgtcg cgctccgctg ctcccgacac  2041 cgagatcgcg ggcaacgtgc cgcccgccgc ggtgtctgcc ttcgcctcgc cgcccggctg  2101 ccgcgcgtcc gcccccggct gcgccgcctg tgcctgcgca tgacccgccg ccgccgcgta  2161 gaacgccacg ccggccgcga tcgcacgcac gcgcgtgctg gttgcccact ccatcttccg  2221 ttgctccaag ttgcaaagcg gccgcgaggg ccgcaccttc atcaa  51 1 mmkvrpsrpl cnleqrkmew atstrvraia agvafyaaaa ghaqaqaaqp gadarqpgge  61 akadtaaggt lpaisvsgaa erdasvglva rrsmtgtktd tpiieipqti nvvtaqqiea  121 tgatdinqaf ryipgfssyg sdnrsdwyaa lrgftptvfv dglqvpntin lsswrvdpym  181 idsiavlrgp tsvlygqgdp gaivdvqskl angerirelg vqvgnyarkq lmfdigdtig  241 kdgtlsyriv gvgrdgnaqt gpladqrvsf apslkwqpna dtsltlaaty lqdwgdtssn  301 flpsrgtvlp npngtisddl ytadanfdhy rkkqwslgyq fehklnpvwt lrqnvrwmhl  361 slddasvygg glddadptma tmtryaglfq fnysrfdvdn qaqakfttgp lshtllfgfd  421 ynrqtttdse wlakgpslnl yrpvytpips difsgpnayp rtdtkttlna fglyvqdqik  481 wrrwvltlgg rqdwtrtsqd dianaasfrq ndhafsgrvg ltylgdygla pylsystsfn  541 pqiglklagg glatptkgrq ieaglrwqpp gknlmlnaav yqinqtnvam snpndptsst  601 fvqvgevrsr gvelsavgnl srelsviaay vyqdvknvra ndntlnkwpv dvprprqias  661 lwadwtwrtg pltgfgvgag vrymsaaaga adnsltvpsy tlfdaalhye lrnwrfalna  721 tnlfnrryva gcqsdavcmy gnqrtviata kynw  502 55 53721329 YP_110314 77 1 atgaacaaga ccaacatcaa cgaacgcatc gcgcgcgtcg cgaagatcgc ggcggcgagc  61 gggttgctcg tcgcgctgct cgccgcgtgc gcggtggggc ccgactaccg gcggcccgat  121 gtcgcgacgc ccgcggcgtt caaggaagcg ccggcgctcg cgccgggcga gcaggccggc  181 acgtggaagg cggccgagcc ggccgacgcc gcgcatcgcg gcgaatggtg gcgcgtattc  241 ggcgatccgg tgctcgatgc gctcgaaacg caggcgcttg ccgcgaacca gaacctgaag  301 gccgcggcgg cgcgcgtcga gcaggcgcgc gcggcgaccc gcgccgcgcg ctcgcagtgg  361 ttcccgcaag tgggcgtggg cttcgggccg acgcgcgagg ggctgtcgtc ggcgtcgcag  421 ttccagccgc agggaagcgg cccgacgaac gcgacgctct ggcgcgcgca gggcacggtg  481 tcgtacgaag cggacctgtt cggccgcgtc ggccgcaacg tcgaagcgtc gcgcgcggac  541 gaagcgcaga gccaggcgct gttccgctcg gtgcagctcg cgttgcaggc ggacgtcgcg  601 cagaactact tcgagctgcg ccggctcgat tccgaccagg atctgtaccg gcgcacggtc  661 gggctgcgcg aggaggcgct caagctcgtg cagcggcgct ttgccgaagg cgacatcagc  721 gagctcgacg tgtcgcgcgc gaagaacgaa ctggcgaccg cgcaggcgga cgcggtgggc  781 gtcgcgcggc ggcgcgccgc atcggagcat gcgctcgcga tcctgctcgg caaggcgccc  841 gcggatttct cgttcagcga gacgccgatc gcgccggtgg tggtgcgcgt gccggcgggg  901 ctgccgtcgg cattgctcga gaggcggccc gacatcgcgg cggccgagcg cgcgatgatg  961 gccgcgaacg cgcgcatcgg cctcgcgaag tccgcgtact tcccgaagct cgacatcacc  1021 ggcgcgttcg gctatgaggc ggcgacgctc ggcaacctgt tcctgtggtc gagccgcacg  1081 ttcctgctcg ggccgttcgc gggcacggcg ctcacgctgc cgatcttcga cggcgggcgc  1141 cgcagcgcgg gcgtcgcgca ggcgcgcgcg aagtacgacg aggaagtcgc gaactaccgg  1201 cagcaggtgc tcgtcgcgtt ccgcgaagtg gaggacaacc ttgccgatct gcgtctgctc  1261 gacgatcaga tccgcgcgca aagcgacgcc gtcaacgcgt cgcggcgcgc ggcgaagctg  1321 tcgcgcacgc agtatcagga aggcgcggtc agctatctcg acgtgatcga cagcgagcgc  1381 tcggtgctcg aatcgcaact gcaatcgaac cagttgacgg gtacgcaggc ggtatcgacg  1441 gtcaacctga ttcgcgcgct cggcggcggc tgggggagcg atgctgcgct cggctcgcgc  1501 gagccgggca agcaggacgt cgcgacgcgt tga  52 1 mnktnineri arvakiaaas gllvallaac avgpdyrrpd vatpaafkea palapgeqag  61 twkaaepada ahrgewwrvf gdpvldalet qalaanqnlk aaaarveqar aatraarsqw  121 fpqvgvgfgp treglssasq fqpqgsgptn atlwraqgtv syeadlfgrv grnveasrad  181 eaqsqalfrs vqlalqadva qnyfelrrld sdqdlyrrtv glreealklv qrrfaegdis  241 eldvsrakne lataqadavg varrraaseh alaillgkap adfsfsetpi apvvvrvpag  301 lpsallerrp diaaaeramm aanariglak sayfpkldit gafgyeaatl gnlflwssrt  361 fllgpfagta ltlpifdggr rsagvaqara kydeevanyr qqvlvafrev ednladlrll  421 ddqiraqsda vnasrraakl srtqyqegav syldvidser svlesqlqsn qltgtqavst  481 vnliralggg wgsdaalgsr epgkqdvatr  503 84 53722229 YP_111214 78 1 atgaagtcgc gttccgacga gttgaagctc ggaaaattca ccaccctctg cagcgtgctg  61 gccgcgagcc cggcattcgc gcaggacgcc gcgccgcccg cggcgagcgc cgatcacgac  121 aaggagctcg cgccgatcca gatcaagggc gccgccgaac acagctacaa agccgacttc  181 tcgtcgtcgg tgaaattcac cgcgccgctc gtcgacacgc cgaaatccgt caccgtgatt  241 ccgcaggaac tgatccacaa cagcggcgcg gccacgctca ccgaagcgtt gcgcaccgtg  301 cccggcatca cgttcggcgc gggcgaaggc ggcaatccgc tcggcgaccg cccgttcatc  361 cgcggctacg acacgcaggg cagcatgttc gtcgacggga tgcgcgacac gggcgcgacg  421 acgcgcgaga tcttcaatac cgagcgcatc gagatcacga aaggctcgga cggcgcgtac  481 ggcggccgcg gcggtgcggg cggcagcatc aacctcgtca cgaaggcgcc gcacctgggc  541 acgacggccg aggcgagcgc ggggctcggc accgaccgct atcgccgctt caccgccgac  601 ggcaactggc agttcgccga tcacgcggcg ttccgcctga acctgatgag ccacaacaac  661 gacgtcgcgg gccgcgacgc cgtgaacaac gagcgctggg gcgtcgcgcc gtcgatcgcg  721 ttcgggctcg gcacgccgac gcgcgtgagc gcgagctatt accacctgtc gaccgacgat  781 ctgcccgacg gcggcattcc gtatttctac acgacggcga acaagccggc gaacgtcgat  841 acgatctatc cggcgaacgt cgaccgccac aacttctacg gcctcgtcaa ccgcgacttc  901 cgcaagacga cgacggacat cggcacgctg cgcatcgagc acgacatcac gccgtcgctg  961 acggtgcgca acaccacgcg ctacacggaa tcgacgcagg actacatttg gacgcagccc  1021 gacgacagcc agggcaacgt ggtcaacggc cgcgtctggc ggcgcaacaa caaccgcaat  1081 agcgcgatca acagcatcgc gaaccagacc gagctgttcg gcgaattccg caccggcccg  1141 ttcaagcaca gcttcacgac cggcatcgag ctgtcgcgcg aatggggcaa gcgcgattcg  1201 tacagcgtcg cgaccggcac cggcaagatc tgccaacagg gcatcggcgc cgcgtcgggc  1261 tacaactgca cgagcctggg gtcgccgaac ccgaacgatc cgtgggcggg ctcgatcacg  1321 cgcaacaacg actacgcgca tgcgcgcacc gtcacgaaat cgctctacgg cttcgacacg  1381 gtcgagctga gcaagcattg gcaggtgaac gccggcgtgc gcgtcgacga ctactcgacc  1441 cgcttcaccg acacccgcgc gaacggcggc aagacctaca cgcgcgacga cacgctcgtg  1501 aactggcagc tcggcctcgt gttcaagccc gcgcgcaacg gcagcgtgta cgcttcgtac  1561 gcgacgtcgt cgacgcccgc gggcgcgctg ctcggcgaag gctcggagac gcagtcgctc  1621 acgccgggcc gcggcggcgt cggcgcgaac gcggatcagc tcgcgccgga gaagaaccgc  1681 agcatcgagc tcggcaccaa atggaacgtg ctcgacgaca agctctcgct cacggccgcc  1741 ctgttccaga tcgacacgac gaacgcgcgc gtcacgctgc cgaacaacca gtacgcgatg  1801 gtcggcaaca agcgcgtgca ggggctcgag ctcggcttcg cggggcagct cacgcgcgcg  1861 tggcaggtgt tcggcggcta cacctacatg aagagcgaac tgcgcgacaa cggcaggaac  1921 accgcggaca acggccatca gtttccgaac acgccgaagc acagcttcac gctgtggacg  1981 aactacgacg tgacgccgaa attcaccgtc ggcggcggcg cgttctacat gtcgaaggta  2041 ttcggcgata ccgcgaacct gcgcgccgtg ccgtcatact ggcgcttcga cgcgatggcg  2101 cagtaccgga tcaacaagaa gctcgacctg cagctgaacg tgagcaacct gttcaaccgc  2161 acgtacttcg atcaggcgta tccggcgcac tacgcgtcga tcgcgccggg ccgctcggcg  2221 ttcgtcacgc tcaacgcgcg ctattga  53 1 mksrsdelkl gkfttlcsvl aaspafaqda appaasadhd kelapiqikg aaehsykadf  61 sssvkftapl vdtpksvtvi pqelihnsga atltealrtv pgitfgageg gnplgdrpfi  121 rgydtqgsmf vdgmrdtgat treifnteri eitkgsdgay ggrggaggsi nlvtkaphlg  181 ttaeasaglg tdryrrftad gnwqfadhaa frlnlmshnn dvagrdavnn erwgvapsia  241 fglgtptrvs asyyhlstdd lpdggipyfy ttankpanvd tiypanvdrh nfyglvnrdf  301 rktttdigtl riehditpsl tvrnttryte stqdyiwtqp ddsqgnvvng rvwrrnnnrn  361 sainsianqt elfgefrtgp fkhsfttgie lsrewgkrds ysvatgtgki cqqgigaasg  421 ynctslgspn pndpwagsit rnndyahart vtkslygfdt velskhwqvn agvrvddyst  481 rftdtrangg ktytrddtlv nwqlglvfkp arngsvyasy atsstpagal lgegsetqsl  541 tpgrggvgan adqlapeknr sielgtkwnv lddklsltaa lfqidttnar vtlpnnqyam  601 vgnkrvqgle lgfagqltra wqvfggytym kselrdngrn tadnghqfpn tpkhsftlwt  661 nydvtpkftv gggafymskv fgdtanlrav psywrfdama qyrinkkldl qlnvsnlfnr  721 tyfdqaypah yasiapgrsa fvtlnary  504 83 53721278 YP_110263 79 1 tcagaaatcc accttcatgc tgaccgcgac gctgaggccc ggggcggtgt atgcgtcgag  61 cacgttcgaa tcggcggcga tgccgcgcac gtccgaccag ttccagtact tgaggtcgaa  121 caggttgtga atgccgaggt aggcgctcac gtgcttgttg aagaggtagc cgccgcgcag  181 atcgacgacg aacgacgacg gcggcgtgaa gcaggtcttt ttctggcacg cggccgacga  241 cacgtcgcgg ccgcgcttgc ccgcctgcca cagcaggtcc gcctgcgcga accagcgctc  301 gctcggctcg tagcgcacgc cgaacacggc ggagaacggg ttgaccgtat cgagcggctc  361 gctcgccgcg ccgttgtcct gcgtcgtgcc cttcgtgaac gccatcgccg tcttcagcgt  421 gaagccattc ggcatcaccc attcggcgcg tccctcgaag ccgtgaatgc gcgcgttcgc  481 gaagttcacg tactggaaca cgagcggatc gttcggccgg ccactgccgc ccaccgtgag  541 ctgagagatg aagttgagat agaggccggc gaaagaggcg acgctgtagc gcagaggccc  601 gtagccggtg ccgagcgtgc cgcgcaggcc cgcttcgagc gtgtcgctcg tctcgggctt  661 caggttcgga ttgccgatcg acgtatagcc atagatcgga ttcgagaagc tgttgttgat  721 ctgatcgggc gtcggcgtgc gaaagccgtg cgcatactgc gcgtagggaa tcagcgcggg  781 cgacacttca tagagcatcg cgacgcgcgg cgacagctcg tgatcgctcg agctgaccgt  841 cttgccggtg aacagcggat cgcccgagct cgggctgagc cgatacgcgt cgaagcgcag  901 gcccggcgtg acgagcagct tgccgaagcc gatctgatcc tgcacgaacg cgccgaacag  961 cgagtagtcg gtgtccggaa acgccttgtt cgggaacgat tcgccggggc tcgcgacggt  1021 gccctcgcgc aggctcttga tgaggtcgat gctgccgtcg acgccgtaca gcagcttgtg  1081 cgcgagcggc ccggtcgcga agccgctctc ggcgaacgcg gagccgccga gcgcgcgctc  1141 gctgtagtgg ttcgagcgcg aacgcgattg gagccgcccg ccgcgcgtct cgaacgcgtc  1201 ctgatcctgc gtcgactcct gatagtagaa ctgcacgtgc gcggtctgga acccgcgcgc  1261 ggcggcgtcg cgcaaatcgt agtcgatact gaagaggttg cgctcgagcc tgtcgttcgc  1321 ggtgaggccg agcgttgtcg gcggattgat cgccgacagc acgttcgtgt cgatccgccg  1381 ccgcaccgtt tcggcggcga gcttgatcgt gtcgcgcgcg gtcggcgtga tcgtcagctt  1441 gccgagcagc gattccgtgt agacatcctg aggattcgcg cgcgtgcgcc gcgtgctcgc  1501 ggaattgtcg tcgccgtgag tgtcgagttc gtggccgcgg cggccggacg cgatcagcat  1561 cgcctgcacg cgatcgttgc cgcccgccgc cgtcacggtc gcgccgatgc tgaggtcgac  1621 cgagtcgtag ctcggccgga acgagaaata ggtctttttt cgatggatcg acagcagatc  1681 ggacggatct ttcgtgatga agttgacggc gcccgtcagg ccgtcgctgc catacagcgc  1741 ggacgccgga ccgcgcagga tctcgatgag cgcgagcgtg tcgagatcgg cgtaatcgcc  1801 gcggccggct tcgagagggc cgaacgagaa cgcgctcggc agccggatgc cgtcttccat  1861 cagcagcacg cggttgcctt cgaggccgcg gatgttgatg ctcgaatcgc cgtcgcggcc  1921 gccgccgagc gcggcgttcg ccgggcggta agaggtgcgt cgcacggtga cgcccggctc  1981 gtagcgcagc gcgtccttga tgttgtcggc ctgctgctcc tcgaggtctg aatcggtgat  2041 caccgatacg ctcgcagagg tgaggctcgc ggccgacgcg gtgcgcgtcg cggtgacggt  2101 gatcggatcg agtgccggcg cgtccttgag tgaggcggcg atcgccgcgt gccgcacctg  2161 cgacgtggat gcggcggatg cggcggccgc gggttcggaa gcggggccgg cggcgcgcgc  2221 ggccgccgca tagaggccga aggccccgaa cagcgcggcg cgaagcggcc gccgcgccaa  54 1 marrplraal fgafglyaaa araagpasep aaaasaasts qvrhaaiaaa rkdapaldpi  61 tvtatrtasa asrtaasysv itdsdleeqq adnikdalry epgvtvrrta yrpanaalgg  121 grdgdssini rglegnrvll medgirlpsa fsfgpleagr gdyadldtla rieilrgpas  181 alygsdgltg avnfitkdps dllsihrkkt yfsfrpsyds vdrsigatvt aaggndrvqa  241 mliasgrrgh eldthgddns astrrtranp qdvytesllg kltitptard tiklaaetvr  301 rridtnvlsa inppttlglt andrlernrf sidydlrdaa argfqtahvq fyyqestqdq  361 dafetrggrl qsrsrsnhys eralggsafa esgfatgpla hkllygvdgs idrikslreg  421 tvaspgesfp nkafpdtdys lfgafvqdqi gfgkllvtpg lrfdayrlsp ssgdplftgk  481 tvsssdhels prvamlyevs palipyaqya hgfrtptpdq innsfsnpiy gytsignpnl  541 kpetsdtlea glrgtlgtgy gplrysvaaf agryrnfisq rtvggsgrpn dplvfqyvnf  601 anarihgfeg raewvmpngf tlktamaftk gttqdngaas epldtvnpfs avfgvryeps  661 erwfaqadll wqagkrgrdv ssaacqkktc ftppssfvvd lrggyrfnkh vsaylgihnl  721 fdrkywnwsd vrgiaadsnv ldaytapgrs vaysmkvdf  505 81 53721627 YP_110612 80 1 atgcggtacg gggtggcggg acctgagaga tcgcggcgcg gcgccgcgcc ggtcgggcgt  61 ttcgggggaa ggcggtggcg ctcgtcgctc tgcctgggcg tgctgatcgc ggcccacccg  121 ggcgcggcgg cgttggccgg cacggccgcg aagagcgacg cgcaaaagaa cgacatgcaa  181 aggaacgaaa acgttcggga agacacccgg cggctcgccg ccggcgcgcc gcctgccggc  241 ggcgagctga aggcgatttc ggtgagcgcg ccgaaggacg cggcggacga tccgtcggtc  301 gcgaccgtcg gcaagatgcc gctcgcgctg cgggagatcg cgcaatcggt cagcgtgacg  361 acgcgcgagc ggatcgagca gcagaacctg ttcagcctcg acgacgtgat gcagcaatcg  421 gccggcgtga ccgtgcagcc gtacgtgctg ctgacgaccg cgtacttcgt gcgcggcttc  481 aaggtcgatt cgttcgagtt cgacggcgtg cccgtcgtgc tcggcgacat ggcgagcgcg  541 ccgcaggaca tgtcggctta cgagcgcgcg gagatcctgc gcggcgcgaa cggcttgctg  601 cacgggtcgg gcaatccggc cgcgaccgtc aatctggtgc gcaagcgccc gcaacaccgg  661 tttgccgcga gcgcgagcgt cggcagttgg ggccgctatc gcgcgcaggc cgacatcggc  721 gggccgctca acccggcggg cacggtgcgc gggcggctcg tcgccgcgta cgaggatcgc  781 ggctttttct acgaccgcgc gaagcaggat acgcgctcga tctacggcat cgccgagatc  841 gacgtggcgc gcgacacgct cgtcaccgtc ggcgcgcaat accagtcggt cgcgtcggtg  901 cccgacatgt ccggcgtgcc gatggcgcgc gacggcacga gcctcgggct gccgcgatcg  961 acgttcctcg acgtcggctg gggccatttc gactgggata cgacgcgcgt gttcggctcg  1021 gtcgagcgca agctcggcgg gggctggaag gcgaaggtga gcggcgagta tcaggcggtg  1081 cgctccgatc tgaaatacgc gggcgcgtac ggcgcgatcg atcgggccac gggcgcgggc  1141 ggcacgctga tgggcagcgc ataccagttc agcagctaca gccgcagtgt cgatgcgaac  1201 gtgcagggcc cggtgcgcgc gttcggcctc acgcatgagc tgctgttcgg cgcgacctac  1261 gcgagcagca gcagcgggca gctcaccgcg ccgctgctgg cgggggccgg cacgccggtg  1321 aacgtgtacc gctggaaccc ggacggcgtg ccggagccgg gcgtcgggcc gtaccggcag  1381 gacatgcaga acgacatctc gcaaaagggc gtctacggcc tcggccgcat caagctcgcg  1441 cagccggtca cgctcgtgct gggcgggcgc ctgagctggt ggaaccagga cagcctcggc  1501 gcgcactaca acgcgggccg ccagttcacg ccgtatggcg ggctgatctg ggatgtcgcg  1561 cgcgactggt cgtggtacgc gagctacgcg gaggtgttcc agccgcagac caagccgacc  1621 tgggacggcg gcatcctgac gccggtgaag ggccgcactt acgagacggg catcaagggc  1681 gagctggcgg gcggcaagct caacgtatcg ctcgcggcgt ttcggatcga tctcgacaac  1741 aatccgcgga tcgacgccac gcgtccgtgc gccgggcgca gctgctacta cgtgaacggc  1801 ggcagcgtgc gcagccaggg gttcgaattc gaggcgaacg gacgcatcac gccgtggtgg  1861 agcgtgtggg cgagctacac gttcgacacg atccgctatg cgaaggatgt cgccaacggc  1921 ggcgcgttcg cggcggagct cacgccgcgc cacctgttgc gcgtgtggac gaactacgac  1981 ctgccgtggc aggagcgccg ctggagcgtc ggcggcggcg tgcaggtgca gagcgatttc  2041 tcggcggcgt cgagcggcgt cacgatgcgc cagggcggct acgcgctcgc gagcgtgcgg  2101 ctcggctatc gctacgacag gcactggcgc gccgcgctga acatcaacaa cctgttcgac  2161 cgcacgtact accagagcct cagccagccc ggctggaaca atcgttatgg ggagccgcgc  2221 aacgtgatgc tgaccgtgcg cgggcagttc tga  55 1 mrygvagper srrgaapvgr fggrrwrssl clgvliaahp gaaalagtaa ksdaqkndmq  61 rnenvredtr rlaagappag gelkaisvsa pkdaaddpsv atvgkmplal reiaqsvsvt  121 trerieqqnl fslddvmqqs agvtvqpyvl lttayfvrgf kvdsfefdgv pvvlgdmasa  181 pqdmsayera eilrgangll hgsgnpaatv nlvrkrpqhr faasasvgsw gryraqadig  241 gpinpagtvr grlvaayedr gffydrakqd trsiygiaei dvardtlvtv gaqyqsvasv  301 pdmsgvpmar dgtslglprs tfldvgwghf dwdttrvfgs verklgggwk akvsgeyqav  361 rsdlkyagay gaidratgag gtlmgsayqf ssysrsvdan vqgpvrafgl thellfgaty  421 assssgqlta pllagagtpv nvyrwnpdgv pepgvgpyrq dmqndisqkg vyglgrikla  481 qpvtlvlggr lswwnqdslg ahynagrqft pyggliwdva rdwswyasya evfqpqtkpt  541 wdggiltpvk grtyetgikg elaggklnvs laafridldn npridatrpc agrscyyvng  601 gsvrsqgfef eangritpww svwasytfdt iryakdvang gafaaeltpr hllrvwtnyd  661 lpwqerrwsv gggvqvqsdf saassgvtmr qggyalasvr lgyrydrhwr aalninnlfd  721 rtyyqslsqp gwnnrygepr nvmltvrgqf  506 88 53720334 YP_109320 81 1 ctagaagcgc agcgtcgcga cgagctcgac cgagcgcgac gggccgagca gccattgcgc  61 gccgccgttc gacgtcgacg ccgcgtacgt gcggttcgcg aggttgcgcg catagaacgc  121 gaggtcgacg tcgcgcgtcg cccgccacgc gagcgacgcg tcgaacaccg tgtaagaagg  181 caccggcacg cggttcgcgt cgtcgccgta gcggggaccg acgtagcgca ggcccgcgtt  241 cgcgcgccag ccgggcgcga acgcccagcc gatccacagg ttcgcgctct gctgcgcgat  301 gtcgtgcggc acgttgccgg cgcgctgcac gaccgtgtcg ccgacgcgct ggtcgaacgc  361 gtcgtagcgc gcgcgcagca acgccgcgtt tgcgtcgacc gtcacaccgc ccggcagccg  421 cacgccgccc gcgagctcga cgccgcgcga cgactggcgg ccgatctgct gcgcgcgcgc  481 cggattcagc ggatcgacgc tgacgaggcc gcgcttgacg atgtcgtaga cggcgagcgt  541 ccagtacgcg cgagcgccgt cgatctcgtg cttgatgccc gcttcccatt gacgcccggt  601 cgcgagcgtg tagttcgcct gcgacgccga cagcgtgacg agcgagccca cgccctgcgc  661 acccgtcgtg tactgcgcgt acgcggcgag gcccggcgcg atctcgtaga cgagcccgct  721 gcgccagccg gtgtgcgcga acgtcttgtc gaacgcgccg cccgcgatca gatcgtcgcg  781 atgaaacgac agatggtcgt aacgcagccc gctcacccac gcgagccgcg gcaggacctc  841 gagccggttc tcgatgaacg ccgcggcctg atgcgtgcgc gtgcgaaagc gcggcagcgt  901 cggatcgggg ctcgcgaacg cgccgggatc gaagccggcg gcggcgacgg tcgattcgcc  961 gcgatacggc gcgttgttcg cgccgtcgaa cgcgatctgg ttgaactcgg cgccgagcac  1021 gagccggttc gcgcggccga acacgcgcga gtcgatgcgt gcggtgaagc gctcgccgaa  1081 ctgccgctcg cgatggaaga tctcgagata gtcgctgcgc gcgacggtgc gcgccgccgg  1141 gtcgagcgca tacgattccg cgttgcgcca atggcggcgc gtcgcgagat agtagagctg  1201 ggcatcgagc gtcacgccgt tgcccgcgcg ataggtcgcg gcaagccgcg tccaggtgtc  1261 gtggtatgcg atcgtcgcgt cgccgacgtt gtagttgcgc tcgcgcagcg cgcgctcgag  1321 cacgccgttc gtcgccggca cgccgaaata ggtcgccggc ttctggcggc cgtagtcgta  1381 atcgagcgtg atcgacagac gcgaatcgac atcgagcttc agcgcgccgc cgacggccgt  1441 cacgtgcgcg tcgccgcgct cgacgaagcc gcgcgtgcgc tcgtcgctca gatggaaacg  1501 ataggaaaga cgcgcgccga gcgcgcccgt cgtatcgagc gccacgcgct tttcgccttg  1561 cgtgccgatg cccgcctgca gcgtcgtcga gcgttcgcgg cgcgggcgcc gggtcacgac  1621 gttggcgacg ccgccgatcg ccccttcgcc gtacagcacc gacgccggcc cgcgcagcac  1681 ctcgatgcgc tcggcggacc acgtcgagaa cgggaacgtg acggtgcccg cgccggggta  1741 gaggcgcacg ccgtcgacaa gcgtcgtcac cgattcctgg ccggcgaagc cgcgcacgct  1801 gagcgccgtg ccgccgttgc cgggcgccgc ggccgtgctg aagcccgtcg cgcgcgtgac  1861 ggcgtcgacg atcgtgcgat cgccgcgcgc ggcgatctgc gcggaggtga tcgcctcgac  1921 gctcgcgggc gtgtcgaggc tcgcgagctt caggcgcgag ccggccgcga gcggcgtaga  1981 cagcggcgac gcgtgcgccc cgtcgctcgt cacgcgcacg gtcggcagtt cgcgatgcgg  2041 gccgcgcgcg gcgtcggccg gttccgcctc ggcggcgagc gcgccggacg ccgtcaccgc  2101 gcacgcgagc gcggtgacgc agcactcgac ggccgtcgag cgacgcgacg cggcggcgaa  2161 gcacgcgtgc acgccatgcg cgacaggcgc gccgtgagcg gcgcgagcag ccgccgcgaa  2221 cccgcgcccg aacgcgcgac ggcgcgacgg tgcatgcggc gcccggctca t  56 1 msraphapsr rrafgrgfaa aaraahgapv ahgvhacfaa asrrstavec cvtalacavt  61 asgalaaeae padaargphr elptvrvtsd gahasplstp laagsrlkla sldtpasvea  121 itsaqiaarg drtivdavtr atgfstaaap gnggtalsvr gfagqesvtt lvdgvrlypg  181 agtvtfpfst wsaerievlr gpasvlygeg aiggvanvvt rrprrerstt lqagigtqge  241 krvaldttga lgarlsyrfh lsdertrgfv ergdahvtav ggalkldvds rlsitldydy  301 grqkpatyfg vpatngvler alrernynvg datiayhdtw trlaatyrag ngvtldaqly  361 ylatrrhwrn aesyaldpaa rtvarsdyle ifhrerqfge rftaridsrv fgranrlvlg  421 aefnqiafdg annapyrges tvaaagfdpg afaspdptlp rfrtrthqaa afienrlevl  481 prlawvsglr ydhlsfhrdd liaggafdkt fahtgwrsgl vyeiapglaa yaqyttgaqg  541 vgslvtlsas qanytlatgr qweagikhei dgaraywtla vydivkrglv svdpinpara  601 qqigrqssrg velaggvrlp ggvtvdanaa llrarydafd qrvgdtvvqr agnvphdiaq  661 qsanlwigwa fapgwranag lryvgprygd danrvpvpsy tvfdaslawr atrdvdlafy  721 arnlanrtya astsnggaqw llgpsrsvel vatlrf  507 81 53722763 YP_111748 82 1 tcagagcttg gtgctcaaac gaacccacgc ggtacggccg ggttcggtga caggcagatt  61 cgccggatag ccgaaaccgg cgttgcccgc gaggttcagg tgctccgcat aagccttgtc  121 gagcacgttg tcgacgccga ccgagatctg caccgtcttg ctcacgttgt actgcgcgtg  181 cagcgacagc acgccgaaac cggcgctcgg accaaagtcc ttgccgacga cgttgccctc  241 gttcagcgcg tagcgatgct gcggcgcaac gacccgccac agcccgcccg ccgaccacgg  301 cccgcgagtg tactcgacgc cgaagcgtgc ctcgagcggc ggcatctgcg gcagcggcgc  361 accgctttgc acgttgcgcc cccacgcata cgcgagcgac ccttcgaagc gccacggcgc  421 gagcggacgc cacgacgcgc ccacctcacc gcccatgatc tgcgcgttga cgttcgtcgc  481 ctgcgtgatc tgtcccatcg ggcccgtcgc atagtcgaac aggatgaagt cctgcacgta  541 gcccgcatag gcggacaccc aggcgtcgag cttgtcgctc ttgtactgcg cgccgatgtc  601 gagctgcgtc gtcttctcgg gcttgatcgc ggagaatgcg ttgatcgaac cgttcgggcc  661 gcgcttggcg gagaacagct cccagtaatc agggaagcgc tgcgcatggc cgatgcccgc  721 gtaccacgtg acgggcagcg acgcgagatc acgctcgtag cgcacgaagc cgctcggcag  781 cacgcgcgag cgaagatcgt cgaacgtcgg attgcgcatg ctcatcttca tgccgcccgt  841 cgtcgcgcgc ttgtcgcgcg cggccgcata gtcgatccgc gcgccgccga tcacgcgcga  901 cgcatcgctc gcataccagg tcagctcgcc gaacgcgccc gcgttccaca tgttcgcctg  961 cggattccac ggcttgtcgc cgtagttctg catccccatc gccgagcgcg agtcgaggcg  1021 gttcgactgc gcatcgacgc ccgtcacgag cttgaacgcg tcggtcaagc gcagcgtcgc  1081 cgcgacgcgc gcgccgagcg tgcggcggcg cacttcggag gccatgcgca tcggcatgct  1141 gctcgtcgga tcgggcatcc gcaacgtgta gttgtccatc acgtgatcgg cttcgttgta  1201 gaagacctgc gcatcgatgc gatcgagcac gtcgccgatg tgcttcttgt cgaacttcag  1261 accgaacgtc tcgcgccgga aatgcgcgcc gtccattccg cggcccgcat agcgcgcgta  1321 gccgtcgccc gtgcccgccg tcagctcgag ccgcgtgttg tcgtcgggcg tccagccgag  1381 cgccgcgtcc gcgttccact tgtcccattg cgacggcacc gtgcggccgt tgccgtcctc  1441 gtagtcctgc gagtgcgcat ggttcgcgct cacgcgcccg tagaagtcgg gcgtgccggc  1501 cgtcacgtcg acgttctgat cgttgcgccc gaacgagccg ccgacgacgc tgccgtcaaa  1561 gcgcatgccc ggcgtcttga agcgcggcgt cacgcgctcg aacagcaccg tgcccgccga  1621 tgcgcccggc ccgtacagca cggtctgcgg ccccttgacg agcgtcacct tgtcgtagct  1681 ctcgggcgcg atgtacgacg tcggcgcgtc catccggccg ggacacgcac cgagcgtcgg  1741 catgccgttc gcgagaatgt tcagccgcga gccgaacatc ccgcgcagca ccgggtcgcc  1801 gttcgtgccg ccgctgcgga tcgacgcgaa gccgggaatc gtcttcagat aatcggcgcc  1861 gtcgctcgcg ggcagcggct ggcgcggcgt cttcggatcg gtgacgacga cgagcggcgt  1921 cgattcgggc gccgcgacga tttcgatcgt cggcagcgtc gtggcgtccg gcgcggcgcc  1981 ggtcgtcgtg cggctcgccg cggcgctcgc cgcttcgcga accgcgccgt ccgatttggc  2041 gctcgccgcg tcggtgtcgg cgcggctcgc tacgccggac gcgccgagcg tgccgtggcc  2101 gtgcacggcc tcgctcgtct gcgcgacggc cggcgcgagg tgaaaggcgc ccgcggcgag  2161 cgcgggaacg gtaagcgtga tcgcgcgccg ccggcggcgc gcgtggtctc cacgcgcggt  2221 gggcgcgtga cgcaggaatg tggatgtcat  57 1 mtstflrhap targdharrr rraitltvpa laagafhlap avaqtseavh ghgtlgasgv  61 asradtdaas aksdgavrea asaaasrttt gaapdattlp tieivaapes tplvvvtdpk  121 tprqplpasd gadylktipg fasirsggtn gdpvlrgmfg srlnilangm ptlgacpgrm  181 daptsyiape sydkvtlvkg pqtvlygpga sagtvlferv tprfktpgmr fdgsvvggsf  241 grndqnvdvt agtpdfygrv sanhahsqdy edgngrtvps qwdkwnadaa lgwtpddntr  301 leltagtgdg yaryagrgmd gahfrretfg lkfdkkhigd vldridaqvf yneadhvmdn  361 ytlrmpdpts smpmrmasev rrrtlgarva atlrltdafk lvtgvdaqsn rldsrsamgm  421 qnygdkpwnp qanmwnagaf geltwyasda srviggarid yaaardkrat tggmkmsmrn  481 ptfddlrsrv lpsgfvryer dlaslpvtwy agighaqrfp dywelfsakr gpngsinafs  541 aikpekttql digaqyksdk ldawvsayag yvqdfilfdy atgpmgqitq atnvnaqimg  601 gevgaswrpl apwrfegsla yawgrnvqsg aplpqmpple arfgveytrg pwsagglwrv  661 vapqhryaln egnvvgkdfg psagfgvlsl haqynvsktv qisvgvdnvl dkayaehlnl  721 agnagfgypa nlpvtepgrt awvrlstkl  508 81 53722050 YP_111035 83 1 ttgcacgcgc cgtgcatgcc gcaattcgcc ccgcaattcg ccccgcgccg cgtcgtcgcg  61 cgcgtggccg ccgccgcgct gttctccgcg tgcgccgcgc cgggcgtatc gggcgcggcc  121 gagccggccg ccgcgacgcg cgcgtacgac attccggcgg gcccgctcga cgcggcgctg  181 acccgcttcg gccgcgaggc cggcatcctg ctgtcgttcc cgggcgagct gaccacgggc  241 ctgcgaagcc ccgggctgca cggccgcgcg gatccggccg cggcgctcga ccggctgctg  301 acgggcacgg ggctcgtcgc gctccggcag ccgagcggcg gctacacgct cgccaggctg  361 cccgggccgg ccgccgccgg cgccgatgcc gcgctcgcgg ccgacacgac gctgcccacc  421 gtcgccgtgc gcgcgagcgg cccgcacgcg gacagctacc ggccgccgcg cgaggcggcg  481 gggctgcgca gcgacgcgcc gctcgccgag gtgccgcaag cggtcgccat cgtcgcgcag  541 caggtgctgc gcgaccagcg cccgcgcaat ctcgacgacg cgctcgcgaa cgtgagcggc  601 atcacgcagg gcaacacgct cggcagcacg caggacacgg tgatgaagcg cggcttcggc  661 gacaaccgcg acggctcggt gatgcgcaac ggaatgccga tcgtgcaggg ccgctcgctg  721 aacgcgacga cggacagcgt cgaagtgctg aagggcccgg cctcgctgct gtacggcatc  781 atggacccgg gcggcgtgat caacgtcgtc acgaagcagc cgcagctcgc gcgccgccac  841 gcgatctcgg cgctcggctc gacgtacggc ggcggcagga acggcggcga gctcacgttc  901 gactcgacgg gcgcgatcgg cgagtcgcgc gttgcatacc ggctgatcgt cgatcagacg  961 aacgaacagt attggcgcaa ctacggcgag caccgcgaga cgctcgtcgc gccttcgctc  1021 gcgtggtacg ggcgcgatac gcaggtggtg ctgtcgtacg agtaccggcg cttcctgatg  1081 ccgttcgatc gcggcaccgc gctcgatccg cgcacgaacg cgccgctcgc gatcccggcg  1141 cgccgccggc tcgacgagcc gttcaacgac atgcgcggcg aatcgaatct cgcgcagctc  1201 gcgatcgatc atcagctcgc gccggactgg aaggtgcacg tcggctacag ctacaaccgg  1261 gagacctacg atgcgaacca gattcgcatc acggcggtcg atccgctcaa gggcacgctc  1321 acgcgcagca acgacgcgac gcacggctcg cgcagcaccg acagctacgg catcgcgtac  1381 gtcgacggcc gcgtgacgct cgccgggatg cggcacgacg tgcagttcgg cgtcgacggc  1441 gaataccggc aggtctaccg cgcggacatg ctgcgccagc cgatcaagac gccgttcagc  1501 tatctgaacc cgacgtacgg gctcgtcccg ccgtcgacga gcgtatcggc ctccgacagc  1561 gaccagagcg acacgctgca caccgcctcg ctgttcttcc aggacagcat ccatctgagc  1621 gagcggtgga tgctcgtcgg cggcgcgcgc tgggtccgct acagccagct tgccgggcgc  1681 ggccggccgt tccaggtcaa cacgaacctg agcggcacga agtggctgcc gcgcgcgggc  1741 atcgtctaca agtggaacga cgcgctgtcg ctgtacggca gctacacgca gtcgctgaag  1801 ccgacctcga agatcgcgcc gatggcgggc ggctacgtga tcgacggctc gaccgcgccc  1861 gaggaaggcg cgtcgtggga gctcggcgcg aagctcgaca tgccgggcgg cctcgccggc  1921 acgctcgcgt tcttcgacat cgacaagaag cacgtgctcg tctcgcaata cgacgacgcg  1981 acgaaccaga ccgcgtggcg cacgtccggc cgcgcgcgct cgcgcgggat cgagctcgac  2041 gtgtcggggc ggatcggcgc gcgctggaac gtgatcgcga gctacgcgta catcgacgcg  2101 aagacgaccg aagatccgct gtacgcgggc aaccggctgt ggaacgtcgc gcggcacacg  2161 gcctcgctcg cggcggtcta cgacgtgggc acggtgctcg gcggcgacga cctgcgcatc  2221 ggcgcggcgg ggcgctacgt cggcgcgcgc ccgggcgatt cggccaacag cttcacgctg  2281 ccggcgtacg cgaccgccga cgcgttcgcg acctacgaca cgcggcttgg caagcagaag  2341 ctgcagttcc agctcaacgt gaagaatctg ttcaaccgca cgtattaccc gtcgagcgtg  2401 aaccggttct tcgtgtcggt gggcgacgcg cggcaggtgt cgctgctgac gacgctgcag  2461 ttctga  58 1 mhapcmpqfa pqfaprrvva rvaaaalfsa caapgvsgaa epaaatrayd ipagpldaal  61 trfgreagil lsfpgelttg lrspglhgra dpaaaldrll tgtglvalrq psggytlarl  121 pgpaaagada alaadttlpt vavrasgpha dsyrppreaa glrsdaplae vpqavaivaq  181 qvlrdqrprn lddalanvsg itqgntlgst qdtvmkrgfg dnrdgsvmrn gmpivqgrsl  241 nattdsvevl kgpasllygi mdpggvinvv tkqpqlarrh aisalgstyg ggrnggeltf  301 dstgaigesr vayrlivdqt neqywrnyge hretlvapsl awygrdtqvv lsyeyrrflm  361 pfdrgtaldp rtnaplaipa rrrldepfnd mrgesnlaql aidhqlapdw kvhvgysynr  421 etydanqiri tavdplkgtl trsndathgs rstdsygiay vdgrvtlagm rhdvqfgvdg 481 eyrqvyradm lrqpiktpfs ylnptyglvp pstsysasds dqsdtlhtas lffqdsihls  541 erwmlvggar wvrysqlagr grpfqvntnl sgtkwlprag ivykwndals lygsytqslk  601 ptskiapmag gyvidgstap eegaswelga kldmpgglag tlaffdidkk hvlvsqydda  661 tnqtawrtsg rarsrgield vsgrigarwn viasyayida kttedplyag nrlwnvarht  721 aslaavydvg tvlggddlri gaagryvgar pgdsansftl payatadafa tydtrlgkqk  781 lqfqlnvknl fnrtyypssv nrffvsvgda rqvsllttlq f  509 27 53718641 YP_107627 84 1 ttactgcgcc gcttgcggcg cgcgcaggta gatctcgacg cggcggtttt gcgcgcggcc  61 ggcttccgtt gcgttgtcgg cgatcgggtt cgacgcgccc atgccttgcg cggacaggcg  121 gttcgccgcg acgccgcgct gcgcgagcgc gttcacgacg ctttgcgcgc gattttgcga  181 cagcgtttga ttgtgcgcgg ccgagcccgt gctgtcggtg tagccgacga ccgacgcggt  241 gatctgcggg ttctggttca gcgtcgtcgc gaggtcgttc agcagcggcg tgaacgccgg  301 cgtgatcgcg tactggtcgg tcgcgaacgt gaccgagctg ggcacgttca gcttgagcga  361 gccgtccggc tgttcggtga cctgcgtgcc cgtttgctgc gccgacggcg cgagcttgtt  421 cttgatcgct tgccagttgt aacccgtcac gccgccgacg agcgcgccga cgccggcgcc  481 gatcgccgcg cccttgccgc cgccagcgag cgcgccgatg cccgcgccga gcgcggcgcc  541 cgtgccggtg ccgacagccg tgttggtgcc ttgctgggtt gcgcaacccg ccagcagggc  601 gccggcgaga gcgaaaacgg acaagcgcgt cgcgattttg gtattcat  59 1 mntkiatrls vfalagalla gcatqqgtnt avgtgtgaal gagigalagg gkgaaigagv  61 galvggvtgy nwqaiknkla psaqqtgtqv teqpdgslkl nvpssvtfat dqyaitpaft  121 pllndlattl nqnpqitasv vgytdstgsa ahnqtlsqnr aqsvvnalaq rgvaanrlsa  181 qgmgasnpia dnateagraq nrrveiylra pqaaq  510 19 53720375 YP_109361 85 1 atgatgtcga agaaacttcg tctggcgttc gcgatgctga tgatcggcgc gctcgcggca  61 tgcaagtcgg gcgtgaagct cgacgagcac gcgaaccagg gcgacgcggt cagcacgcaa  121 ccgaatcccg aaaacgtcgc gcaggtgacc gtcgatccgc tgaacgatcc gaacagcccg  181 ctcgcgaagc gcagcgtgta cttcgatttc gacagctact cggtgcagga ccaataccag  241 gcgctgctgc agcaacacgc gcaatacctg aagagccatc cgcaacgcca catcctgatc  301 cagggcaaca ccgacgagcg cggcacgagc gagtacaacc tcgcgctcgg ccaaaagcgt  361 gcggaagccg tgcgccgcgc gctgtcgctg ctcggcgtcg gggatgcgca gatggaagcc  421 gtgagtctcg gcaaggaaaa gccggttgcg ctcggccacg acgaagcgtc gtgggcgcag  481 aaccgccgtg cggacctcgt ctatcaacag taa  60 1 mmskklrlaf amlmigalaa cksgvkldeh anqgdaystq pnpenvaqvt vdplndpnsp  61 lakrsvyfdf dsysvqdqyq allqqhaqyl kshpqrhili qgntdergts eynlalgqkr  121 aeavrralsl lgvgdaqmea vslgkekpva lghdeaswaq nrradlvyqq  511 24 53720132 YP_109118 86 1 atgaataaac tttcaaagct cgcgttcatt gcagctaccg cagttatggc tgcatccgct  61 tcggcacagt cggtgccggc gtcgcgacaa gccgtgaatg acaactgggt gaatggcacg  121 ggcgaatggg tgtggatgaa cggcacgaac gagctctgct ggcgcgatgc gttctggacg  181 ccggccaccg ccaacgccaa gtgcgatggc gcactggtcg cccaggcacc ggcaccggcg  241 ccggtcgcac cggttgctcc ggccatcacg agccagaaga ttacgtacca agccgatacg  301 ctgttcgact tcgacaaggc cgtcctgaag ccggccggca agcagaagct tgacgaactg  361 gccgcgaaga tccagggcat gaacgtcgaa gtggtcgtgg ccacgggcta cacggaccgc  421 atcggttcgg acaagtacaa cgaccgtctg tcgctgcgcc gcgcgcaagc cgtcaagtcg  481 tacctcgtca gcaagggtgt cccggcgaac aaggtctaca cggaaggcaa gggcaagcgc  541 aacccggtca cgggcaacac ctgcaagcag aagaaccgca agcagctcat cgcctgcctc  601 gcaccggacc gccgcgtgga agtcgaagtg gtcggcacgc aggaagtgca gaagacgacc  661 gttccggcgc agtaa  61 1 mnklsklafi aatavmaasa saqsvpasrq avndnwvngt gewvwmngtn elcwrdafwt  61 patanakcdg alvaqapapa pvapvapait sqkityqadt lfdfdkavlk pagkqkldel  121 aakiqgmnve vvvatgytdr igsdkyndrl slrraqavks ylvskgvpan kvytegkgkr  181 npvtgntckq knrkqliacl apdrrvevev vgtqevqktt vpaq  512 40 53720170 YP_109156 87 1 atgaaaaaga ccctcatcgt tgccgctctt tccggcgttt tcgcaacggc cgctcacgcg  61 caaagcagcg tgacgctgta cggcctgatc gacgccggca tcacctacac gaacaaccaa  121 ggcggccaca gcgcatggtc gcaatccacc ggctcggtca acggcagccg ctggggcctg  181 cgcggcgccg aggatctcgg cggcggcctg aaggcgattt tcgtgttgga aaacggcttc  241 ggcatcaata acggcacgct gaagcagaac ggccgcgagt tcggccgtca ggcgttcgtc  301 ggcctgtcgc acgagcaata cggcgcgctg acgctcggcc gtcaatacga cagcgtcgtc  361 gactacctcg ggccgctgtc gctgacgggc acgcaattcg gcggcacgca gttcgcccac  421 ccgttcgaca acgacaacct gaacaattcg ttccggatca acaacgcggt caagtacacg  481 agcgtgaact gggcgggcct gaaattcggc gcgttgtacg gcttctcgaa caacaatcag  541 ttcgcgaaca accgcgccta tagcgcgggc gtatcgtaca gctacgccgg cttcaacatc  601 ggcgccggct acctgcagtt gaacaacaac ttcggcccga cggtctccaa cgcatccggc  661 gcggtcgcgc tcgacaacac gttcgtcggc aagcgccagc gcgtgttcgg cggcggcctg  721 aactacacgt tcggcccggc aacggccggc ttcgtgttca cgcaatcgcg cgtcaaccgc  781 gcgacggcaa tcggcgcggg cgcatcgggc gtgtcgagcg gcattgcgct cgacggcacg  841 ttcatgcgct tcaacaacta cgaagtgaac gcgcgctacg cgatcacgcc ggcatggacg  901 gtggccggtt cgtacacgta caccgccggc ttcatcgaga accaccaccc gggctggaac  961 caattcaacc tgcaaacggc ctacgcgctg tccaagcgca cggacgtgta cctgcaaggc  1021 gtgtatcaga aggtcaacaa cgacggcacg ggcctcggcg cgtacatcaa cggtatcggc  1081 ggcatgtcgt cgacggaaaa acagatcgcc gtcacggccg gcctgcgtca ccgcttctaa  62 1 mkktlivaal sgvfataaha qssvtlygli dagitytnnq gghsawsgst gsvngsrwgl  61 rgaedlgggl kaifvlengf ginngtlkqn grefgrqafv glsheqygal tlgrqydsvv  121 dylgplsltg tqfggtqfah pfdndnlnns frinnavkyt svnwaglkfg alygfsnnnq  181 fannraysag vsysyagfni gagylqlnnn fgptvsnasg avaldntfvg krqrvfgggl  241 nytfgpatag fvftqsrvnr ataigagasg vssgialdgt fmrfnnyevn aryaitpawt  301 vagsytytag fienhhpgwn qfnlqtayal skrtdvylqg vyqkvnndgt glgayingig  361 gmsstekqia vtaglrhrf  513 44 53721967 YP_110952 88 1 atgaaaaagc acgtcatttc cgcagccgca ttgctggcct tcgccgcgcc ggttttcgcc  61 caaagcagcg tcacgctgta cggcgtgatc gacgaaggct tcaactacac gagcaacgtg  121 aacgtcaacg gcgtcggcaa gagcaactac cagctcgcga gcggtttcgt gcagggcagc  181 cgctggggcc tgcgcggctc ggaagacctg ggcggcggcc tgaaagccat cttcacgctc  241 gaaaacggtt tcgacgtgaa caacggccgg ctcggccagg gcggccggat gttcggccgc  301 caggcgttcg tcggcctgtc gcacgcgcaa tacggctcgc tcacgctcgg ccgccagtac  361 gattcgctcg tcgactacct cgcgccgttg accgcgaacg gcaactgggg cggcacgctg  421 ttctcgcatc cgttcgacaa cgacaacacg gacaactcgt tccgcgtgaa caacacggtc  481 aagtacgcga gcccggactg gaacggtctg caagtcggcg gcacgtacag cttcagcaac  541 gcgacgggct tctcgaacaa ccgccagtac agcatcggcg ccgcgtacac gctgggcggc  601 ctgcaacttg cggccgcgta cctgcaggcg aacaacccgg gcaagacggc gggcggcgcg  661 atcgccgaca acgacgcgaa cttcacggcg gaccgcctgc gcatcttcgg cggcggcgtc  721 aactacacgt tcggcccggc gacggtcggt ttcgtctaca cgaagaccga cgtgaagaac  781 ccggtctcga cggtctatct gccgacggcg acgttcgcgg gcctcggcct gagcgcgacg  841 aagttccaga acttcgaaat caacggcaaa taccagctga cgccggcgct cttcatcggc  901 gcgcagtacg tgtacacgga cggcaagttc gatgcggccg cgggcagcgt caagccgaag  961 taccacacgg tcggcctgat ggcggactac aacctgtcga agcgcaccga cgtctatctg  1021 cagggcgcgt accagaaggt cgcgggcgac aagacgggca cgatcgcgga tggcggctac  1081 gtcgtcggga cggacggccc ctcggcgtcg gcgaaccagt tcgcggttcg cgcggcgatc  1141 cgtcacaagt tctga  63 1 mkkhvisaaa llafaapvfa qssvtlygvi degfnytsnv nvngvgksny qlasgfvqgs  61 rwglrgsedl ggglkaiftl engfdvnngr lgqggrmfgr qafvglshaq ygsltlgrqy  121 dslvdylapl tangnwggtl fshpfdndnt dnsfrvnntv kyaspdwngl qvggtysfsn  181 atgfsnnrqy sigaaytlgg lqlaaaylqa nnpgktagga iadndanfta drlrifgggv  241 nytfgpatvg fvytktdvkn pvstvylpta tfaglglsat kfqnfeingk yqltpalfig  301 aqyvytdgkf daaagsvkpk yhtvglmady nlskrtdvyl qgayqkvagd ktgtiadggy  361 vvgtdgpsas anqfavraai rhkf  514 39 53721903 YP_110888 89 1 atgaacaaga ctctgattgt tgcagcagtt gctgcatcgt tcgcaaccgt cgctcacgcg  61 caaagcagcg tcacgctgta cggtgtgctc gacgcgggca tcacgtacca aagcaacgtc  121 gcgacgccgt cgggctcggg caagtcgctg tggtcggtcg gcgccggcgt cgaccaaagc  181 cgtttcggtc tgcgcggctc ggaagacctg ggtggcggcc tgaaggcgat cttcacgttg  241 gaaagcggct tcaacatcgg taacggccgc ttcaacaacg gtggcggcat gttcaaccgt  301 caagcgttcg tcggtctgtc gagcaactac ggcaccgtca cgctgggccg tcagtacgac  361 gcaacccaag actacctgtc gccgctgtcg gcaacgggca cctggggcgg cacgtacttc  421 gcgcaccggc tcaacaacga ccgcctgaac acgaacggcg acgtcgcggt gaacaacacg  481 gtcaagttca cgagcgcgaa ctacgccggc ctgcaattcg gcggcacgta ctcgttctcg  541 aacaactcgc aattcgcgaa caaccgtgca tacagcgcgg gcgcttcgta ccagttccaa  601 ggcctgaagg tcggtgcggc gtactcgcaa gctaacaacg ctggcgcgaa caccacgggc  661 gcaacggatc cgctgaccgg cttcaacatc ggcggcacga acgcagcaag catccaaggc  721 cgttcgcgcg tgtacggcgc cggcgcaagc tacgcttacg gcccgctgca aggcggcctg  781 ctctggacgc aatcgcgtct cgacaacctg gcaaacggcg cgccgaccac ccgtgccgac  841 aactacgaag caaacgtgaa gtacaacctg acgccggctc tgggtctggg tgttgcttac  901 acgtacacga acgcgaaggc gaacggcgaa agcactcact ggaaccaagt tggcgtccag  961 gccgactacg cgctgtcgaa gcgcaccgac gtgtacgcac aagccgtgta ccagcgttcg  1021 tcgaagaacg cgaatgcgtc gatctacaac ggcgacctca gcacgccgtt cagcacgtcg  1081 atcaaccaaa ccgcagcgac ggttggtctg cgtcaccgct tctaa  64 1 mnktlivaav aasfatvaha qssvtlygvl dagityqsnv atpsgsgksl wsvgagvdqs  61 rfglrgsedl ggglkaiftl esgfnigngr fnngggmfnr qafvglssny gtvtlgrqyd  121 atqdylspls atgtwggtyf ahrlnndrln tngdvavnnt vkftsanyag lqfggtysfs  181 nnsqfannra ysagasyqfq glkvgaaysq annaganttg atdpltgfni ggtnaasiqg  241 rsrvygagas yaygplqggl lwtqsrldnl angapttrad nyeanykynl tpalglgvay 301 tytnakange sthwnqvgvq adyalskrtd vyaqavyqrs sknanasiyn gdlstpfsts  361 inqtaatvgl rhrf  515 55 53722699 YP_111684 90 1 atgaagaaac tcgctctgtc taccctctcg ctcgcgctgc tgggcgcagc cggtgcagct  61 caggctcaaa gcagcgtcac gctgtacggc gtgattgata cgtcgatcac ttatgtccac  121 ggcaacgacg gcaaggccaa caacgcctgg ctgatgggca gcggcaacct gcaaggcagc  181 cgctggggcc tgaagggcac cgaggatctc ggcgccggcc tgaaggcgat cttccagttg  241 gaaaatggct tcaactcgaa cgacggcacg ctcggccaag gcaagcgcat gttcggccgc  301 caggcgttcg tcggtctgca gcatgaccag tacggcacgc tgacgctcgg ccgtcagtac  361 gatccgctcg tcgacctcgt gcaaccggtg acggccgaca actacttcgg cagcctgttc  421 gccaccccgg gtgacgtcga caacaacgac aacagcctgc gcgtgaacaa cacggtcaag  481 tacacgtcgc ccgtgttcgc cggcttccag ttcgaagccc tgtacggctt cagcggcatc  541 gcgggctcgc cgggccaagg ccagacgtgg tcggccgccg ccgcatacaa caacggcccg  601 atcggcgtcg cggccggcta cttctacacg tcgaacccgt cgccgacggc cggctcgcgc  661 tcgacgtgga gcggctcgtc cgacgcgatc ttcgacggcg cgatcaacag cggctacacg  721 agcgcgaagt cgatcggcat cgcacaggtg gccggccagt acgtgttcgg cccggtcacg  781 gtcggcctcg gctacagcaa cgcgcaatac aagccggacg gcttctcggg cttctcgtcg  841 accgagaagt acaacacggg ccgcggcttc gtgacgtacc aggccacgcc ggcgctgctg  901 ctcggcctcg gctacgcata cacgaaggcg agcggcgaca cggacgcgaa gtaccaccaa  961 gtgtcgatcg gcgcggacta cgcgctgtcc aagcgcacgg acgtctacct cgccggtgcg  1021 taccagcacg cgagcggcac gcagcgcgtc gatgcgacga cgacgcaaac ggcgcaggca  1081 tcgatcggct cgtacggcgt gaacggtacg aagtcgcaag agatggtggc tctgggcctg  1141 cgccacaagt tctaa  65 1 mkklalstls lallgaagaa qaqssvtlyg vidtsityvh gndgkannaw lmgsgnlqgs  61 rwglkgtedl gaglkaifql engfnsndgt lgqgkrmfgr qafvglqhdq ygtltlgrqy  121 dplvdlvqpv tadnyfgslf atpgdvdnnd nslrynntvk ytspvfagfq fealygfsgi  181 agspgqgqtw saaaaynngp igvaagyfyt snpsptagsr stwsgssdai fdgainsgyt  241 saksigiaqv agqyvfgpvt vglgysnaqy kpdgfsgfss tekyntgrgf vtyqatpall  301 lglgyaytka sgdtdakyhq vsigadyals krtdvylaga yqhasgtqrv datttqtaqa  361 sigsygvngt ksqemvalgl rhkf  516 58 53719415 YP_108401 91 1 tcaagcacac ccatcgccgc cgcacccggc gcgcgaccag cccccgccca gcgcgcgata  61 cagcgtgatc gcgttcgtga gccgcagctg cttcaggcga atcagctcct gcccggactc  121 gaacgtgctg cgctgcgcgt cgagcagctc gagatagctc gccacgccgc tgtcgtagcg  181 ccgctgcgcg agccgcagcc gctcggcgtc cgcaccgtag accgcctgct gcgcggcaag  241 ctgcgcgtcg atctgatcgc gcgccgcgag cgcatcggcg acttcgcgaa acgccgtctg  301 gatcgtcttc tcgtattcgg cgaccgcgat atgcttgcgc gcgtccgcca cgtcgagatt  361 cgcacgattg cgcccgcccg cgaaaatcgg cagcgtgagc cgcggcgcga acgtccacac  421 gctcgagccg gccgagaaga gtcccgagaa cgcgtcgctc accgagccga cgtcggtcgt  481 gagcgcgatg cgcgggaaga acgccgcgcg cgccgcgccg atgttcgcgt tcgccgcgac  541 gaggcgctgc tcggcctgcc ggatgtccgg gcgctgctcg agcagatccg acgacagccc  601 cggcgacacc cgcgccaccg cgagcgcgtc gagcgcgggc gcatcggcgg gcagcgccgt  661 catgaagtcg cccgcgagca gcttcagcgc gctcgccgcc tgcgtgtgct cgcgctcgag  721 cgccgccttc gacgcgcgcg ccgacgcgac gagcatctcg gccgtgcgca gctcgatcgc  781 cgtgctcgtg ccggccgcgt aacggcgctg cgtgagcgcg taggccgcgt cgcgcgcggc  841 gagcgtgcgt tcggcgagcc ccagttgatc gacgagcgcg cgctcggtca cgtacgcgga  901 cgcgacttcg gcgatcaggc tgatgcgcgc cgcgcgctgc gcctcggcgg tcgcgaagta  961 ctcggcgagc gccgcgtccg agaggctctt cacgcggccg aacagatcga tctcgaacgc  1021 gctcacgccg acactcgcgc gatacagcga gctcgtcgcg ctctcgcgca gcaccgggtc  1081 gtaaagccgc gtgcgctcgt agccgaggct gccgtcgatc gacggcagcc ggtccgcgcg  1141 cgcgacgccg tagaggccgc gcgcttcctg gatacgcagc gtcgcgatcc gcaggtcgcg  1201 attgttcgcg agcgccgcgt cgatcagcgc gcgcagcgcc ggatcggtga aatacgcgcg  1261 ccagtcgtcg agccgcgcgt cgcgggcgtc gcgcccggcg tcgccgcggc gcgcgtccgg  1321 catcgcgcgc gggccgccgg cggccgccgg gcccgcctcg cgcggcgcgc ccgtcgccgt  1381 cgaccaataa tgcgccggca cgggcgcggc cggccgctcg tagcgcggcg cgagcgtgca  1441 gcccgcgacg agcgcggccg cgaccgcgag cggccaggcc cgcggcccgt tcggccgccg  1501 cgctgcgcgc gcgccgcgcc gccgggcccg gggtttcatc atcgctttca c  66 1 mkammkprar rrgaraarrp ngprawplav aaalvagctl apryerpaap vpahywstat  61 gapreagpaa aggprampda rrgdagrdar darlddwray ftdpalrali daalannrdl  121 riatlriqea rglygvarad rlpsidgslg yertrlydpv lresatssly rasvgvsafe  181 idlfgrvksl sdaalaeyfa taeaqraari sliaevasay vteralvdql glaertlaar  241 daayaltqrr yaagtstaie lrtaemlvas araskaaler ehtqaasalk llagdfmtal  301 padapaldal avarvspgls sdlleqrpdi rgaegrlvaa nanigaaraa ffprialttd  361 vgsysdafsg lfsagssvwt faprltlpif aggrnranld vadarkhiav aeyektiqta  421 frevadalaa rdgidaqlaa qqavygadae rlrlaqrryd sgvasylell daqrstfesg  481 qelirlkqlr ltnaitlyra lgggwsragc ggdgca  517 55 53718454 YP_107440 92 1 atgaagcgaa aacatgcttt gactgcactc gcagtcgcgc tgctcgccgc gggctgcacg  61 ctcgcgccgc gctacgagcg tccggccgcg ccggtgtcgg gcgcgttccc cgccgacggc  121 gtctatgccg cgcagccggg cgccgcgccc ggcgcgcgca gcgcgaacgg ccaggcggcc  181 gtcgatatcg gctggcgcga gttcttcgtc gatccgtgcc tgcagcggct gatcgagatc  241 gcgctgaaga acaaccgcga cctgcgcgtg tcggtgctca acgtcgaggc gtcgcgcgcg  301 cagtatcaga tcacgcgcgc ggggctcttc ccgacgttga gcggcaccgg cacgggcacg  361 atccagcgca cgccggccgg cgtgtcgatc accggccagc cgctcatctc gcggacctac  421 aacgtcggcg tctccgcgtc gtgggagctc gacctgttcg gccgcgtgca gagcctgaag  481 gatcaggcgc tcgcgcaata cttcgcgacc gcgcaggcgc gcaaggccgc ggagatctcg  541 ctcgtcgcga gcgtcgccga tcagtacctg acgctgctgt cgaccgacga tctgctgcag  601 gtcacgcaga acacgctgaa gtcggcgcgc gcgtcctacg atctgacgaa gctgcagttc  661 gacaacggca ccggctcgga gctcgacctg cgccaggcgc agacggtggt cgagaccgcg  721 ctcgcgagcc agcaggcgca ggcgcgcgcc cgcgcgcagg cgctcaacgc gctcgtgctg  781 ctgatcggcg agccgctgcc cgacgatctg ccggccggcc tgccgctcaa tgcgcagaac  841 ctgctcaccg acattccggc cgggctgccg tccgatctgc tcacgcggcg gcccgacatc  901 atgcaggccg aggagacgct gcgcgcggcg aacgcgaaca tcggcgcggc gcgcgcggcg  961 ttcttcccga agatctcgct caccagcgcg ttcggcaccg cgagcccgac gctcggcggc  1021 ctgttcaagg cgggcacggc ggcgtggtcg ttcgcgccga acatcgcgct gccgatcttc  1081 gagggtgggc agaacatcgc gaacctcgat ctcgcgcacg tgcagaagcg catcgagatc  1141 gcgaactacg agaaggcgat ccagagcgcg tttcgcgagg tgtcggacgg gcttgccgcg  1201 cgcggcacgt acgatcagca gatcgcggcg ctcgagcgca acgagcacgc gcagcagcgc  1261 cgctacgatc tgtcggacct gcgctacagg aacggcgtcg acagctatct gtcggtgctg  1321 accgcgcaga cggacctgta ttcggcgcag cagcagttga tcagcgcgcg gctcgcgcgc  1381 tggacgaacc tcgtggacct gtatcgcgcg ctgggcggcg ggtggctcga gcgcgcgggc  1441 gagacgccgc gcccggcgga cgcgcccgtc gattacggca aggcggcgcc cgcgccggcg  1501 gcggccgtgc cggctgcgtc ggccagcgcg ccggcggcgg gctga  67 1 mkrkhaltal avallaagct lapryerpaa pvsgafpadg vyaaqpgaap garsangqaa  61 vdigwreffv dpclqrliei alknnrdlrv svlnveasra qyqitraglf ptlsgtgtgt  121 iqrtpagvsi tgqplisrty nvgvsaswel dlfgrvqslk dqalaqyfat aqarkaaeis  181 lvasvadqyl tllstddllq vtqntlksar asydltklqf dngtgseldl rqaqtvveta  241 lasqqaqara raqalnalvl ligeplpddl paglplnaqn lltdipaglp sdlltrrpdi  301 mqaeetlraa nanigaaraa ffpkisltsa fgtasptlgg lfkagtaaws fapnialpif  361 eggqnianld lahvqkriei anyekaiqsa frevsdglaa rgtydqqiaa lernehaqqr  421 rydlsdlryr ngvdsylsvl taqtdlysaq qqlisarlar wtnlvdlyra lgggwlerag  481 etprpadapv dygkaapapa aavpaasasa paag  518 19 53721406 YP_110391 93 1 ttattccggc gagcccatca tcgactgctg atagttctga atgccgacct tgccgatcag  61 gtcgatttgc gtctcgagcc agtcgatgtg ctcctcggta tcgtcgagga ttttctcgaa  121 gatttcgcgc gacacgtaat cacgcaccga ttcgcaataa gcgatggctt ccttgcacgt  181 ggcctgcgat acctgttcga gcttcaggtc gcatttcagg atttcctcgg tttcctcgcc  241 gacgagcagc ttgtgcagat cctgcaggtt cggcaggccg tcgagcatga acacgcgctc  301 gatcagccag tccgcgtgct tcatttcgcc gatcgattcg tcgtattcgt gcttgccgag  361 tttttcgagg ccccagtgtt tgtacatccg cgcatgcagg aaatattgat tgatcgcggt  421 cagttcgttt ttcaactgcg cgttcagata ttcgatgact ttcttgtcgc cttgcat  68 1 mqgdkkviey lnaqlknelt ainqyflhar mykhwglekl gkheydesig emkhadwlie  61 rvfmldglpn lqdlhkllvg eeteeilkcd lkleqvsqat ckeaiayces vrdyvsreif  121 ekilddteeh idwletqidl igkvgiqnyq qsmmgspe  519 8.5 53721404 YP_110389 94 1 tcaggccgcc ttgcgttcgt agaacgtgac ggcgaccggt acggccgcag ggcgctcggc  61 accgcaacgg ctcgcacaaa cgccctgttc cgccatgatc tcgcgcacgg tttcctcgca  121 cttgccgcag caggtggcga ccccgagttc gaactggagt tcttcgaacg tgttcacgcc  181 ttccgcgagg gatgcgcgaa tcttccgatc ggaaacggac ttgcacacgc agacgatcat  69 1 mivcvcksvs drkiraslae gvntfeelqf elgvatccgk ceetvreima eqgvcasrcg  61 aerpaavpva vtfyerkaa  520 36 53722329 YP_111314 95 1 tcacttgaaa cggatcgtcg ggttgatgac cgcgtacagc acgtcgacgc tcaggttgat  61 caggatgaac tccagcgaga acagcagcac gatcgcctgg atcaccgggt agtcgcgcat  121 cgtcaccgca tcgacgagca ggcgcccgag ccccggccag ttgaacaccg cctcgacgac  181 gatcgagccg ccgagcagga agccgaactg cagccccatc atcgtgacga ccggaatcat  241 cgcgttgcgc aggcagtgct tgagcacgac catcggctcg cgcacgccct tcgcgcgcgc  301 ggtgcgcacg aaatcctcgt tcagcacctc gacgaacgac gcgcgcgtga agcgcgccat  361 cacggcggcc acggccgcgc cgagcgtcac cgacggcggc acgtagctct tccacgagcc  421 gtccggcacg acgggcagcc agccgagctt caccgagaac acctccatca gcagcatgcc  481 gagcgcgaac gccggaaacg agatgcccga caccgcgagc gtcatgccga tgcggtccgg  541 ccagcggttg cgccacacgg ccgacgcgat gccgatcgcc atgccgaaga gcgtcgccca  601 ggccatgctg acgacggtca gcgtcagcgt cggcatgaag cgctcgccga tctcggtcgc  661 tacgggccgc ttgctgcgcg tcgagacgcc gaagtcgccg tgcgcgatcc gcgcgaagaa  721 gctcgcgaac tgcgccggca gcggcttgtc gaggccgaga tcggcgcgca cgagcgcgac  781 ggtcgcgtcg tcggcctcgg ggccggccgc gagccgcgcg ggatcgcccg gcagcaggtg  841 gacgaacagg aacaccagca ccgcgacgca cgccagcgtc ggcagcagcc cgaacagtcg  901 tttgaccaga aaattcagca t  70 1 mlnflvkrlf gllptlacva vlvflfvhll pgdparlaag peaddatval vradlgldkp  61 lpagfasffa riahgdfgvs trskrpvate igerfmptlt ltvvsmawat lfgmaigias  121 avwrnrwpdr igmtlavsgi sfpafalgml lmevfsvklg wlpvvpdgsw ksyvppsvtl  181 gaavaavmar ftrasfvevl nedfvrtara kgvrepmvvl khclrnamip vvtmmglqfg  241 fllggsivve avfnwpglgr llvdavtmrd ypviqaivll fslefilinl svdvlyavin 301 ptirfk  521 42 53721737 YP_110722 96 1 atgaaaaagc gcactgcggt cgcgatgacg gccgccggac tggcggctgt cgccaccgcg  61 cacgctcaga gcagcgtgac gctttacggc atcgtcgata acggcatcgc gtatcaaagc  121 agcagcacgt cgctcggctc gacgacgggc ggccgctcgg cggtgaagat gtcgacgggc  181 gtgtgggcgg gcagccgctt cggcctgaag ggcagcgagg atctcggtgg cggctcgaag  241 gcgatcttcc agctcgaatc gggcttcagc acggccaacg gcacgtcgca gttcgcgggc  301 ggcatcttca cgcgccaggc gtgggtcggc ctgaccaacc cgacgtacgg cacgctgacg  361 gcgggccgcc agtacaccgc gtactacacg ctgctgtcgc cgtatagccc gacgacctgg  421 ctcaccggct atttcggcgc gcatccgggt gacatcgatt cgctcgatac cagctaccgc  481 acgaacaatt cgctcgtcta cctgtcgccg aagttctacg gcttcacgtt cggcggctcg  541 tatgcgttcg gcggacagcc gggcagcgtg aacgccggct ccacgtggag cgcgggcatc  601 cagtacatga acggtccgct cggcatcgcg gcggcgttcc agcgcgtgaa caactcgacg  661 tcgggcggcg gcgactgggg cgcgaattcg acgacgtcga acggcggcgc gcagacggcc  721 gtgtcggcga tcaacaacgg ctacaagacc gcgcaggcgc agcagcgcgt cgcggtgacg  781 gccggctatc agttctcgtc cgcatgggac atctcggtgt cgtactcgaa cgtgcagtac  841 atcccgggcg tgaactcgac gttccgcaac acggcgatct tcaacacggc gggcgcggtg  901 ctgcacttca agccgtcggc tcagtgggac ttcgcgggcg gctacgcgta cacgcgcgcg  961 acccagtcga acggcatcac gagcgccgcg cagtaccatc agttcacgct gtcgcagtac  1021 tacagcctgt cgaagcgcac gggcctgtac gcggtcgaag cgtatcagcg cgcgaacggc  1081 aagacgctcg cgggcggcaa gatcatcgac gcgaccgcgt cgatcggcga cggcttcaac  1141 acgtcgccgt cgtcgtcgcg cagccaggtg ggcgtcggcg tcggcctgat ccaccgtttc  1201 tga  71 1 mkkrtavamt aaglaavata haqssvtlyg ivdngiayqs sstslgsttg grsavkmstg  61 vwagsrfglk gsedlgggsk aifqlesgfs tangtsqfag giftrqawvg ltnptygtlt  121 agrqytayyt llspyspttw ltgyfgahpg didsldtsyr tnnslvylsp kfygftfggs  181 yafggqpgsv nagstwsagi qymngplgia aafqrvnnst sgggdwgans ttsnggaqta  241 vsainngykt aqaqqrvavt agyqfssawd isvsysnvqy ipgvnstfrn taifntagav  301 lhfkpsaqwd faggyaytra tqsngitsaa qyhqftlsqy yslskrtgly aveayqrang  361 ktlaggkiid atasigdgfn tspsssrsqv gvgvglihrf  522 56 53719527 YP_108513 97 1 atgaaaacct tcccgttgtc cgcttgccgg accgccgtgg ccgtcgcggt cgccgcgctc  61 gcgctcgcgg gatgcgcgaa ctatttcggc ctcaagagcg acaaggcgat cgctcccgcg  121 gcgcagttcg aaagcgcgcg gagcctgccc gcgcagggcg gccaatggcc gtcgctcgac  181 tgggcgaacc agttcggcga tccgcaattg ccgaagctga tcgacgaggc gctcgaaggc  241 aatccgacga tcgcgcaggc gcaggcgcgc atcgcgaagg cgtcgtcgta catcgagtcg  301 tcgcgctcga cgctgctgcc gaaggccgac gcgaagtact cgtggacccg cgagctgtac  361 tcgggcaacg cccttttccc gcccccgtac ggcggccagt ggtacagcga gaacaacgcg  421 ctcgcgagcg cgtcgtggga gctcgatctg tggggcaaga accgcgcgcg cctgaatgcg  481 gccgtgtcgc aggaaaaggc cgccgaagcc gacctgcagc aggcgcgcgt gacgctcgcg  541 acgtcggtcg cgcgcacgta caaccagctc gcgcaactgt acgcgctgcg cgacatcgcc  601 gggcgcgaga tctcgaaccg gcagacggtc ggcaagatca ccgacggccg cgtcggcgcg  661 ggcctcgaca cgaacgtcga gcgccagacc gcgcgcggca acatcgcgac gagccagtcg  721 acgctgtccg atctcgacgg ccagatcacg aacgtccgct atcagctcgc cgcgctgctc  781 ggcaaaggtc cggaccgcgg gctgcagatc gccgcgcccg tgctgagcgc gggcggcgcg  841 gtcgcgctgc cggagaacct gcccgccgat ctcgtgtcgc gccgcccgga tctcgtcgcc  901 gcgcgctggc aggtcgaagc ggcgatgcac gacgtgaagg aagcgaaagc cgagttcttt  961 ccggacatca acctcgcggc gggcttcggc ttcgacgcgt tcggctgggg ccgattcctg  1021 aacttcgcga gccgccaggc gcagttcggc cccgcgatcc acctgccgat cttcgacggc  1081 ggcgcgctgc gcgcgcaatt gaagggccgc tacgcggact tcgatctgtc ggtcgcgaac  1141 tacaaccaga cgctcatcaa cgcgctgaac gacgtcgcga cgcaagtcgc gtcgattcgc  1201 tcggtcgaca cgcaaatggg cgacgcgcag cgcgcgctcg atgcgtcgac gcgcgcctac  1261 gagctcgcgg tgatccgcta caaggcgggc ctgtcgccgc agttgcaggt gctcaacgcg  1321 gacagcaacc ggctcgccgc cgagcagacg gtgacgaacc tgaagatgcg ccgccgcgac  1381 ctgcagatcg gcctcgtgaa ggcgctcggc ggcggcttcg acgcggccgg cacgcggctc  1441 gccgcgcccg cccccgcctc ggcgccggcc gcgcccgcgc agcacgcatc gaactga  72 1 mktfplsacr tavavavaal alagcanyfg lksdkaiapa aqfesarslp aqggqwpsld  61 wanqfgdpql pklidealeg nptiaqaqar iakassyies srstllpkad akyswtrely  121 sgnalfpppy ggqwysenna lasasweldl wgknrarlna aysqekaaea dlqqarvtla  181 tsvartynql aqlyalrdia greisnrqtv gkitdgrvga gldtnverqt argniatsqs  241 tlsdldgqit nvryqlaall gkgpdrglqi aapvlsagga valpenlpad lvsrrpdlva  301 arwqveaamh dvkeakaeff pdinlaagfg fdafgwgrfl nfasrqaqfg paihlpifdg  361 galraqlkgr yadfdlsvan ynqtlinaln dvatqvasir svdtqmgdaq raldastray  421 elavirykag lspqlqvlna dsnrlaaeqt vtnlkmrrrd lqiglvkalg ggfdaagtrl  481 aapapasapa apaqhasn  523 85 53720164 YP_109150 98 1 tcacagcgac catttcaact ctccataaaa agtgcggccc ggataaggat ggaacacgta  61 gtagcggcga tcggtcacgt tgtcgatgcc gaacgacgca agccagtgcc gatcgaaccg  121 atagcgcgcc ttcaggtcga cgaccatgaa cgagctggtg ccgccgtata cgcccggatt  181 cacgtcgctg ttgtcgagcg cgttgtactg ccgccccgaa tagcgaacgc cgacgctcgt  241 catccaatgc tcgtcgaagc ggtacgacgc gagcaggttc gcgcgcatcc gcggaatccg  301 cggccagcgc gcgccgacgt agttcggatt ggccgcatcg gcaagcgtct gcgcattcgt  361 cgcggacacg ttcgcgtcga cgtcgagccc cttgagcgcg acgtcctgcc ccgagaacgc  421 gagctcgacg ccgcgcaccc gcacgcggtc gacgttcgag acgttcgtgt acgtcgacgc  481 gccggcaagc gtggtctggc tgtagatcga attgcgcaga tcgctctgga acacgctcgc  541 gcgcacgacg ccgaagccga cgtcgcgctc ggcggtgaaa tcccagtcga tcgccttttc  601 cggctgcaga ttcgggttgt tgttgacgat cgcgttgttc gagatcgtgc cctggaacag  661 ctcggcgacc gtcggaaagc gcgtgcccgt cgcgaacgac aaccgcaggc gccacgcgct  721 ggccggctgc cattcgagcg agagcttcgg cgagaacgcg gtcgcgccgc gctccgcgta  781 gccgagcgtc gcggtcgcgc cgccgagctc gccgccgtat gcatcccagc gctcgtagcg  841 caggccgagc gtcgcgagcc agccgggcgc gacgcgccac gcgtcctgcg catacagcgc  901 ctgcgtgcgc gtgttgccgc gatagcggtt cacgagcgtc gtcggcaccg cgttttgcca  961 gtcggccgtg ttgtaggtcg cgttgcgcag gaaatacgta tcgaagtggt agccgaacga  1021 aaaacggtgc ccgcgcacgt cgggcgactc cgcgcgcaag tcgacggtgc gccagccggt  1081 gccgtcgcca tggaacaccg tgcccggccc gccgtcccag gcgccgggcg gtgcgccgga  1141 cgcgctgcgc agcacgtcgc gcgacacttc gtaggcggac gcggtggccg acagcttcca  1201 gccggacgcg agccgcgcgt cgagcccgag cccgtacagc cagttctcct ggtcgcccgt  1261 ctgcggcgcg aacgcgcccg gcgacaccgt gtagttgcgc ccgccgaacg acacgttgcc  1321 gccgtacacc gggttgcccg ccgcgtcgcg caggaacgtg tcgccgtgct gccggtagtg  1381 attctcccag tggccgagcg tgaccgtcgc gtcgacgtga tcggtgaacg cgtagccgaa  1441 gcgcaacgtc tcgttgagct gctcggtgcg ctcgatcgtc tgcgcgccga cgatcgtccg  1501 cggccggccg ttcggcccga tgtcggagac ggcccccgtc accggcacgg gtgcgccgag  1561 cctcggatcg aacgcgccgt tcgggctcgc gtattgcatc ggctggctgt cgttctcgag  1621 ccggtcgagc gacagcgcat accagaaacg tccgacgcga tcggcgatgc gcgcgctctg  1681 gtgattgccg ccgaagctgt cggcgaagcc gtagccgtcg cgatagcgct gcgtgaagaa  1741 ctgcgtcgac accgacgcct cgagccgctg cggcttgtgc gtggtgatct gcacggtcga  1801 gccgatcgcg ttgcccgggt agagcgcgga aaacgggccg tacagcacgt ccacgcgtgc  1861 gatgtcgtcg ggctggatca gcgaccagcg cggcggatac gaatagctgg aaccgagcag  1921 attggacagc aggatgccgt ccgcatagac gagtccgcgc gcgctctgca gctcattgaa  1981 gtctcggccg gcgaacactg aattgcggtc gccgatgtag cgccggcgca ccatcaggtt  2041 cggcgcgtac ttgagcgcgt cttcggtcgt cacgttgacg tgcgaatcga tctgctcgcg  2101 cgtgagcgcc tcgacgacgc cgggcgcgtc gggcgcgaac gcggcgcgct gcgcggtgac  2161 cttgacggta tccaaggttt tcccggatgc ggtcatctcc gcggcggcgg ccggcgttga  2221 atcggcaggc acggcggcgg ccgtcgaagc cgcgtgggcg gcgggccagg cgagcgccgc  2281 ggcgcaggcg gccgcgagcc gcctgcgcgc gccgggcgcg gcaagttgat gaaacat  73 1 mfhqlaapga rrrlaaacaa alawpaahaa staaavpads tpaaaaemta sgktldtvkv  61 taqraafapd apgvvealtr eqidshvnvt tedalkyapn lmvrrryigd rnsvfagrdf  121 nelqsarglv yadgillsnl lgssysyppr wsliqpddia rvdvlygpfs alypgnaigs  181 tvqitthkpq rleasystqf ftqryrdgyg fadsfggnhq sariadrvgr fwyalsldrl  241 endsqpmqya spngafdprl gapvpvtgav sdigpngrpr tivgaqtier teqlnetlrf  301 gyaftdhvda tvtlghwenh yrqhgdtflr daagnpvygg nvsfggrnyt vspgafapqt  361 gdqenwlygl gldarlasgw klsatasaye vsrdvlrsas gappgawdgg pgtvfhgdgt  421 gwrtvdlrae spdvrghrfs fgyhfdtyfl rnatyntadw qnavpttlvn ryrgntrtqa  481 lyaqdawrva pgwlatlglr yerwdaygge lggatatlgy aergatafsp klslewqpas  541 awrlrlsfat gtrfptvael fqgtisnnai vnnnpnlqpe kaidwdftae rdvgfgvvra  601 svfqsdlrns iysqttlaga stytnvsnvd rvrvrgvela fsgqdvalkg ldvdanvsat  661 naqtladaan pnyvgarwpr iprmranlla syrfdehwmt svgvrysgrq ynaldnsdvn  721 pgvyggtssf mvvdlkaryr fdrhwlasfg idnvtdrryy vfhpypgrtf ygelkwsl  524 78 53722869 YP_111854 99 1 tcagaatgtc gtgcgcatcc cgatgctcac gctgcgcccg ccctgcggcg cgatgttgcg  61 caccaccgag ctcgcgtagc ggatgtcctg gttcgtcagg ttgtcgccgc gcagataggc  121 gagccagttc gtcgcgccga cgcgcaactt gtaggtgagc acgacgccga gcgacgtgta  181 gccgtcggtc gcgagatcgt gctcgggcac gcgatgctgc gcccacgcat gcgtgagctg  241 cgcgcgcgca ccgaacgggc cgtagccgta atcggccgcg agcgtcgcgc gcagcggcgc  301 gatccgcggc agcggctcgc ccgtgtccgc gttgcgcgcg tgcgtgtagt cggcgctcag  361 ctcgagatcg acgcgatgcc cgcgccgctc gaacgcgcgc cacctgcctt cgagctcgac  421 gccgtagaac tccgcgcgca cgccgcgata gacggcctcg cgcagcgcgt cgtcggcgcc  481 cggggccacg ggcacgccgt cgtcgtcgac gagccgtccg gtgtcgtatt cggcgaggta  541 gttccgcagg cggctgtaga acacgccgat gctgccgcgg ttcggaccgc tcgcatagcg  601 cagcgcgaga tcggtcgaca ccgccttctc cttctgcgca tcgggccggc cgatcaggta  661 ctgccccgtc gcgccgtgcg ggccgttcgc gtacagctcg tagaaggtcg gcgcgcgctc  721 cgtgtacgac acgttgcccg cgagcgacca cgcgggcgcg agctgataca gcgcgcccgc  781 cgacacgctg cccgcattga aatcgcgcga gcgcgcgaag ccgaacttgt cgtcgccgtt  841 cgcgctcgga tcgagccgca cgtgctcgat gcgcgcgccg gcggacagct tcagcgcgtc  901 ggtcgcctgc cactgctcga ggccgaacag cgcgacgctc gtcgtgcgcg tggtaggcgc  961 gagcgcctcg ccgccgagcg cggagaacgt gttctgtccg acctgcacgc cgagcgcgcc  1021 ttcgaacggg ccgagcttgc ggtgccgcgc ctcgacgcgc gcctcgtagc cgtgattgcg  1081 aaacgtcgtg cccgtcacgc cgtcctcgat ttccttatgc tggtaattcg tgtagccgaa  1141 gtcgaatttc agctgcgaga acggcccgcg cagattgcgc acctccgacg cgagcgcgac  1201 gcgctcctgc cgcatctgaa gccgcgcgtc ggtttcggcg acggagccgt agttcgattc  1261 gtagccgctg tacgatgcgc cgacgtagcc gtccgcccac gtgtacgagc cgcccgccgc  1321 gccgccgtaa cggcggccgt cgctgttcgg cagcttgccg tacggctcgc tggcgtcctc  1381 gccgtcgagc gcgcgctggc gcgccgaatg cgcatggccc ggaatccgca gcgcgtcggt  1441 ctcgcggccg aacgcgtcga gatgaaacgc gaagcggccg ttgccgcctt cgacgagcgc  1501 cgcgcccgca cgcgcattgt tcgcgccgcc gtagctcgcg tcgagcgcgc ccgagacgcc  1561 cgtgatcgct tcgcgcggaa tccggttgtc gatcgtgttg acgacgccgc cgaccgcgtt  1621 gccgccgtac agcaacgccg ccggcccgcg cacgatctcg atgcgctcga cgctcagcgg  1681 atcctgcggc accgcgtgat cgtaggacag cgacgacgcg tcgtacgcgg cgacgccgtt  1741 ctgcaggagg cggatgcgat cgccgtccat cccgcggatg atcggacggc cgacgagcgg  1801 gccataggtc gtcgtggaca cgccgggcag gccgttcagc gtgtcgccga gcgaatcggt  1861 gcggcgcagc gtcagcgcgt cgccggacag cgacgcggtg ggcgagctca gcgccgacgc  1921 gccgagcggg ttcgcggtca cgaagatcgg cgcgagcgcc gtcgcgggcg gcgcgtccga  1981 tcggcgctcg gcgggcggcg cgcccgtctc gccatgcgcg aggctcgcgg cgagcagcag  2041 cgacaaggga tgcagcctgc gagcgaaagg cggcgcgata cgtcggtggt catccat  74 1 mddhrriapp farrlhplsl llaaslahge tgappaerrs dappatalap ifvtanplga 61 salssptasl sgdaltlrrt dslgdtlngl pgvstttygp lvgrpiirgm dgdrirllqn  121 gvaaydassl sydhavpqdp lsverieivr gpaallyggn avggvvntid nripreaitg  181 vsgaldasyg gannaragaa lveggngrfa fhldafgret dalripghah sarqraldge  241 dasepygklp nsdgrrygga aggsytwadg yvgasysgye snygsvaetd arlqmrqerv  301 alasevrnlr gpfsqlkfdf gytnyqhkei edgvtgttfr nhgyearvea rhrklgpfeg  361 algvqvgqnt fsalggeala pttrttsval fgleqwqatd alklsagari ehvrldpsan  421 gddkfgfars rdfnagsvsa galyqlapaw slagnvsyte raptfyelya ngphgatgqy  481 ligrpdaqke kaystdlalr yasgpnrgsi gvfysrlrny laeydtgrlv dddgvpvapg  541 addalreavy rgvraefygv elegrwrafe rrghrvdlel sadytharna dtgeplpria  601 plratlaady gygpfgaraq lthawaqhrv pehdlatdgy tslgvvltyk lrvgatnwla  661 ylrgdnltnq diryassvvr niapqggrsv sigmrttf  525 43 53718452 YP_107438 100 1 atgcgcgtcg aacgggttcc ataccgctta atcactgtcg cgacggctgc cgttttcctg  61 gccgcgtgcg gaaaaaaaga atcggctccg ccccctcaaa cgcccgaagt cggcgtcgtc  121 accgtccagc cgcagcccgt gccggtcgtc tccgaactgc cgggccgtac gagcgcctat  181 ctggtcgcgc aggtgcgcgc acgggtcgac ggcatcgtgt tgcggcgcga gttcacggaa  241 ggcagcgacg tcaaggccgg ccagcgcctg tacaagatcg atccggcacc ctatatcgcg  301 caattgaaca gcgcgaaggc gacgctcgcg aaggcgcagg cgaacctcgc gacgcagaac  361 gcgctcgtcg cgcgctacaa ggtgctcgtc gccgcgaacg cggtcagcaa gcagcagtac  421 gacgatgcgg tggccgcgca agggcaggcg gccgccgacg tgggcgcggg caaggccgcc  481 gtcgagaccg cgcagatcaa cctcggctat acggatgtcg tctcgccgat cacgggccgc  541 gtcggcatct cgcaggtcac gccgggcgcg tacgtgcagg cgagccaggc gacgctgatg  601 tcgaccgtcc agcagctcga tccggtctac gtcgatctca cgcagtcgag cctcgacggc  661 ctgaagctgc gccaggacat ccagagcggg cgcatcaaga cggaaggccc gggcgcggcg  721 aaggtcacgc tgattctcga ggacggcaag ccgtacccgg agcggggcaa gctgcagttc  781 agcgatgtca cggtcgacca gacgacgggc tcggtcacga tccgcgcgat cttcccgaac  841 aagcagcgcg tgctgctgcc gggcatgttc gtgcgcgcgc gcatcgaaga gggcgtcaac  901 gagaacgcgt tcctcgttcc gcagatcggc gtcacgcacg atccgaaggg ccaggcgatc  961 gcgatgatcg tcgacggcaa gggcaaggtc gagccgcgcg tgctggtcac gggcggcacg  1021 cagggccaga actgggtggt cgagagcggc ctgcaggcgg gcgaccgcgt gatcgtgcag  1081 ggcatcgaca aggtgcgccc gggcatgacc gtgaaggccg ccgaggctca actgccggcc  1141 gcggcggccg gcgcgtcggg tgccgcgccc gcgggcggct cgcccgccca ggccgcggcg  1201 gcgagcgcgg ccgcgtcggg tgccgcgccg tcgagcgctg ccgccgcgtc gagcgcgcaa  1261 taa  75 1 mrvervpyrl itvataavfl aacgkkesap ppqtpevgvv tvqpqpvpvv selpgrtsay  61 lvaqvrarvd givlrrefte gsdvkagqrl ykidpapyia qlnsakatla kaqanlatqn  121 alvarykvlv aanavskqqy ddavaaqgqa aadvgagkaa vetaqinlgy tdvvspitgr  181 vgisqvtpga yvqasqatlm stvqqldpvy vdltqssldg lklrqdiqsg riktegpgaa  241 kvtliledgk pypergklqf sdvtvdqttg svtiraifpn kqrvllpgmf vrarieegvn  301 enaflvpqig vthdpkgqai amivdgkgkv eprvlvtggt qgqnwvvesg lqagdrvivq  361 gidkvrpgmt vkaaeaqlpa aaagasgaap aggspaqaaa asaaasgaap ssaaaassaq  Bm10229-600 ID MW Protein GI# Protein Locus# SEQ ID NO: Sequence  601 81 124386268 YP_001026287 121 1 ttaccagttg tatttcgcgg tcgcgatcac ggtgcgctgg ttgccgtaca tgcacaccgc  61 atccgactgg cagccggcca cgtagaggcg gttgaacagg ttcgtcgcgt tgagcgcgaa  121 acgccagttg cgcagctcgt agtgcagcgc cgcgtcgaac agcgtgtagc tcggcaccgt  181 gagcgaattg tcgaccgcgc ccgccgccgc gctcatgtag cgcacgcccg cgccgacgcc  241 gaagcccgtg agaggcccgg tgcgccacgt ccagtcggcc cacagcgacg cgatctggcg  301 cggcagaggc acgtcgacgg gccacttgtt cagcgtgttg tcgttcgccc gcacgttctt  361 cacgtcctga tagacgtacg cggcgatcac cgacagctcg cgcgacaggt tgcccaccgc  421 gctcagctcg acgccgcgcg agcgcacctc gcccacctgc acgaacgtgc tgctcgtcgg  481 gtcgttcgga ttgctcatcg cgacgttcgt ctggttgatc tggtagacgg ccgcgttcag  541 catcaggttc ttgccgggcg gctgccagcg caggcccgcc tcgatctggc ggcccttggt  601 cggcgtcgcg agcccgccgc ccgcgagctt caggccgatc tgcggattga acgacgtcga  661 atagctgagg tacggcgcga gcccgtagtc gccgagatac gtgaggccca cgcgcccgct  721 gaacgcgtgg tcgttctgcc tgaagctcgc cgcgttcgcg atgtcgtcct gcgacgtgcg  781 cgtccagtcc tgccggccgc cgagcgtgag cacccagcgc cgccacttga tctggtcctg  841 cacgtacagg ccgaatgcgt tcagcgtcgt cttcgtgtcg gtgcgcgacg cgttaggccc  901 gctgaagatg tcggacggaa tcggcgtgta gacgggccgg tacaggttca ggctaggccc  961 cttcgcgagc cattcgctat cggtcgtcgt ctggcggttg tagtcgaagc cgaacagcag  1021 cgtgtgcgaa agagggcccg tcgtgaattt cgcctgcgcc tggttgtcga cgtcgaagcg  1081 gctgtagttg aactggaaca ggcccgcgta gcgcgtcatc gtcgccatcg tcgggtctgc  1141 gtcgtcgagg ccgccgccgt agacggacgc gtcgtcgagc gacaggtgca tccagcgcac  1201 gttctgccgc agcgtccaca ccgggttcag cttgtgctcg aactgatagc cgagcgacca  1261 ctgcttcttg cggtaatggt cgaagttcgc atcggccgtg tacaggtcgt ccgagatcgt  1321 gccgttcgga ttcggcagca cggtgccgcg cgacggcagg aagttgctcg acgtgtcccc  1381 ccagtcctgc aggtacgtcg cggcgagggt gagcgacgtg tccgcgttcg gctgccattt  1441 gagcgacggc gcgaacgaca cgcgctggtc ggcgagcggc cccgtctgcg cgttgccgtc  1501 gcggccgacg ccgacgatcc ggtacgacag cgtgccgtcc ttgccgatcg tgtcgccgat  1561 gtcgaacatc agttgcttgc gcgcgtagtt gccgacctgc acgccgagct cgcggatgcg  1621 ctcgccgttc gcgagcttgc tctgcacgtc gacgatcgcg ccgggatcgc cctgcccgta  1681 cagcaccgac gtcgggccgc gcagcacggc gatgctgtcg atcatgtacg gatcgacgcg  1741 ccagctcgac aggttgatcg tgttcggcac ctgcagcccg tcgacgaaca cggtcggcgt  1801 gaagccgcgc agcgccgcgt accagtccga acggttgtcc gacccgtagc tcgagaagcc  1861 cggaatgtag cggaacgcct gattgatgtc ggtcgcgccc gtcgcctcga tctgctgcgc  1921 ggtgacgacg ttgatcgtct gtggaatctc gatgatcggc gtatccgttt tcgtgcccgt  1981 catgctgcgc cgcgcgacga ggccgacgct cgcgtcgcgc tccgctgctc ccgacaccga  2041 gatcgcgggc aacgtgccgc ccgccgcggt gtctgccttc gcctcgccgc ccggctgccg  2101 cgcgtccgcc cccggctgcg ccgcctgtgc ctgcgcatga cccgccgccg ccgcgtagaa  2161 cgccacgccg gccgcgatcg cacgcacgcg cgtgctggtt gcccactcca tcttccgttg  2221 ctccaagttg caaagcggcc gcgagggccg caccttcat  101 1 mkvrpsrplc nleqrkmewa tstrvraiaa gvafyaaaag haqaqaaqpg adarqpggea  61 kadtaaggtl paisysgaae rdasvglvar rsmtgtktdt piieipqtin vvtaqqieat  121 gatdinqafr yipgfssygs dnrsdwyaal rgftptvfvd glqvpntinl sswrvdpymi  181 dsiavlrgpt svlygqgdpg aivdvqskla ngerirelgv qvgnyarkql mfdigdtigk  241 dgtlsyrivg vgrdgnaqtg pladqrvsfa pslkwqpnad tsltlaatyl qdwgdtssnf  301 lpsrgtvlpn pngtisddly tadanfdhyr kkqwslgyqf ehklnpvwtl rqnvrwmhls 361 lddasvyggg lddadptmat mtryaglfqf nysrfdvdnq aqakfttgpl shtllfgfdy  421 nrqtttdsew lakgpslnly rpvytpipsd ifsgpnasrt dtkttlnafg lyvqdqikwr  481 rwvltlggrq dwtrtsqddi anaasfrqnd hafsgrvglt ylgdyglapy lsystsfnpq  541 iglklagggl atptkgrqie aglrwqppgk nlmlnaavyq inqtnvamsn pndptsstfv  601 qvgevrsrgv elsavgnlsr elsviaayvy qdvknvrand ntlnkwpvdv prprqiaslw  661 adwtwrtgpl tgfgvgagvr ymsaaagavd nsltvpsytl fdaalhyelr nwrfalnatn  721 lfnrryvagc qsdavcmygn qrtviataky nw  602 55 124383029 YP_001025939 122 1 atgaacaaga ccaacatcaa cgaacgcatc gcgcgcgtcg cgaagatcgc ggcggcgagc  61 gggttgctcg tcgcgctgct cgccgcgtgc gcggtggggc ccgactaccg gcggcccgat  121 gtcgcgacgc ccgcggcgtt caaggaagcg ccggcgctcg cgccgggcga gcaggccggc  181 acgtggaagg cggccgagcc ggccgacgcc gcgcatcgcg gcgaatggtg gcgcgtattc  241 ggcgatccgg tgctcgatgc gctcgaaacg caggcgcttg ccgcgaacca gaacctgaag  301 gccgcggcgg cgcgcgtcga gcaggcgcgc gcggcgaccc gcgccgcgcg ctcgcagtgg  361 ttcccgcaag tgggcgtggg cttcgggccg acgcgcgagg ggctgtcgtc ggcgtcgcag  421 ttccagccgc agggcagcgg cccgacgaac gcgacgctct ggcgcgcgca gggcacggtg  481 tcgtacgaag cggacctgtt cggccgcgtc ggccgcaacg tcgaagcgtc gcgcgcggac  541 gaagcgcaga gccaggcgct gttccgctcg gtgcagctcg cgttgcaggc ggacgtcgcg  601 cagaactact tcgagctgcg ccggctcgat tccgaccagg atctgtaccg gcgcacggtc  661 gggctgcgcg aggaggcgct caagctcgtg cagcggcgct ttgccgaagg cgacatcagc  721 gagctcgacg tgtcgcgcgc gaagaacgaa ctggcgaccg cgcaggcgga cgcggtgggc  781 gtcgcgcggc ggcgcgccgc atcggagcat gcgctcgcga tcctgctcgg caaggcgccc  841 gcggatttct cgttcagcga gacgccgatc gcgccggtgg cggtgcgcgt gccggcgggg  901 ctgccgtcgg cattgctcga gaggcggccc gacatcgcgg cggccgagcg cgcgatgatg  961 gccgcgaacg cgcgcatcgg cctcgcgaag tccgcgtact tcccgaagct cgacatcacc  1021 ggcgcgttcg gctatgaggc ggcgacgctc ggcaacctgt tcctgtggtc gagccgcacg  1081 ttcctgctcg ggccgttcgc gggcacggcg ctcacgctgc cgatcttcga cggcgggcgc  1141 cgcagcgcgg gcgtcgcgca ggcgcgcgcg aagtatgacg aggaagtcgc gaactaccgg  1201 cagcaggtgc tcgtcgcgtt ccgcgaagtg gaggacaacc ttgccgatct gcgtctgctc  1261 gacgatcaga tccgcgcgca aagcgacgcc gtcaacgcgt cgcggcgcgc ggcgaagctg  1321 tcgcgcacgc agtatcagga aggcgcggtc agctatctcg acgtgatcga cagcgagcgc  1381 tcggtgctcg aatcgcaact gcaatcgaac cagttgacgg gcacgcaggc ggtatcgacg  1441 gtcaacctga ttcgcgcgct cggcggcggc tgggggagcg atgcggcgct cggctcgcgc  1501 gagccgggca agcaggacgt cgcggcgcgt tga  102 1 mnktnineri arvakiaaas gllvallaac avgpdyrrpd vatpaafkea palapgeqag  61 twkaaepada ahrgewwrvf gdpvldalet qalaanqnlk aaaarveqar aatraarsqw  121 fpqvgvgfgp treglssasq fqpqgsgptn atlwraqgtv syeadlfgrv grnveasrad  181 eaqsqalfrs vqlalqadva qnyfelrrld sdqdlyrrtv glreealklv qrrfaegdis  241 eldvsrakne lataqadavg varrraaseh alaillgkap adfsfsetpi apvavrvpag  301 lpsallerrp diaaaeramm aanariglak sayfpkldit gafgyeaatl gnlflwssrt  361 fllgpfagta ltlpifdggr rsagvaqara kydeevanyr qqvlvafrev ednladlrll  421 ddqiraqsda vnasrraakl srtqyqegav syldvidser svlesqlqsn qltgtqavst  481 vnliralggg wgsdaalgsr epgkqdvaar  604 83 124382070 YP_001024918 123 1 ttggcgcggc ggccgcttcg cgccgcgctg ttcggggcct tcggcctcta tgcggcggcc  61 gcgcgcgccg ccggccccgc ttccgaaccc gcggccgccg cgccgccggc cgccgcatcc  121 gccgcatccg ccgcatccac gtcgcaggtg cggcacgcgg cgatcgccgc cgcgcgcaag  181 gacgcgccgg cactcgatcc gatcaccgtc accgcgacgc gcaccgcgtc ggccgcgagc  241 cgcaccgcgg cgagcgtatc ggtgatcacc gattcagacc tcgaggagca gcaggccgac  301 aacatcaagg acgcgctgcg ctacgagccg ggcgtcaccg tgcgacgcac cgcgtaccgc  361 ccggcgaacg ccgcgctcgg cggcggccgc gacggcgatt cgagcatcaa catccgcggc  421 ctcgaaggca accgcgtgct gctgatggaa gacggcatcc ggctgccgag cgcgttctcg  481 ttcggcccgc tcgaagccgg ccgcggcgat tacgccgatc tcgacacgct cgcgcgcatc  541 gagatcctgc gcggtccggc gtccgcgctg tatggcagcg acggcctgac gggcgccgtc  601 aacttcatca cgaaagatcc gtccgatctg ctgtcgatcc atcgaaaaaa gacctatttc  661 tcgttccggc cgagctacga ctcggtcgac cgcagcatcg gcgcgaccgt gacggcggcg  721 ggcggcaacg atcgcgtgca ggcgatgctg atcgcgtccg gccgccgcgg ccacgaactc  781 gacacgcacg gcgacgacaa ttccgcgagc acgcggcgca cgcgcgcgaa tcctcaggat  841 gtctacacgg aatcgctgct cggcaagctg acgatcacgc cgaccgcgcg cgacacgatc  901 aagctcgccg ccgaaacggt gcggcggcgg atcgacacga acgtgctgtc ggcgatcaat  961 ccgccgacaa cgctcggcct caccgcgaac gacaggctcg agcgcaaccg cttcagtatc  1021 gactacgatt tgcgcgacgc cgccgcgcgc gggttccaga ccgcgcacgt gcagttctac  1081 tatcaggagt cgacgcagga tcaggacgcg ttcgagacgc gcggcgggcg gctccaatcg  1141 cgttcgcgct cgaaccacta cagcgagcgc gcgctcggcg gctccgcgtt cgccgagagc  1201 ggcttcgcga ccgggccgct cgcgcacaag ctgctgtacg gcgtcgacgg cagcatcgac  1261 cgcatcaaga gcctgcgcga gggcaccgtc gcgagccccg gcgaatcgtt cccgaacaag  1321 gcgtttccgg acaccgacta ctcgctgttc ggcgcgttcg tgcaggatca gatcggcttc  1381 ggcaagctgc tcgtcacgcc gggcctgcgc ttcgacgcgt atcggctcag cccgagctcg  1441 ggcgatccgc tgttcaccgg caagacggtc agctcgagcg atcacgagct gtcgccgcgc  1501 gtcgcgatgc tctatgaagc gtcgcccgcg ctgattccct acgcgcagta tgcgcacggc  1561 tttcgcacgc cgacgcccga tcaggtcaac aacagcttct cgaatccgat ctatggctat  1621 acatcgatcg gcaatccgaa cctgaagccc gagacgagcg acacgctcga agcgggcctg  1681 cgcggcacgc tcggcaccgg ctacgggccg ctgcgctaca gcgtcgccgc gttcgccggc  1741 cgctatcgca acttcatctc gcagcgcacg gtaggcggca gtggccggcc gaacgatccg  1801 ctcgtgttcc agtacgtaaa cttcgcgaac gcgcgcattc acggcttcga gggacgcgcc  1861 gaatgggtga tgccgaatgg cttcacgctg aagacggcga tggcgttcac gaagggcacg  1921 acgcaggaca acggcgcggc gagcgagccg ctcgatacgg tcaacccgtt ctccgccgtg  1981 ttcggcgtgc gctacgagcc gagcgagcgc tggttcgcgc aggcggacct gctgtggcag  2041 gcgggcaagc gcggccgcga cgtgtcgtcg gccgcgtgcc agaaaaagac ctgcttcacg  2101 ccgccgtcgt cgttcgtcgt cgatctgcgc ggcggctacc gcttcaacaa gcacgtgagc  2161 gcctacctcg gcattcacaa cctgttcgac cgcaagtact ggaactggtc ggacgtgcgc  2221 ggcatcgccg ccgattcgaa cgtgctcgac gcatacaccg ccccgggccg cagcgtcgcg  2281 gtcagcatga aggtggattt ctga  103 1 marrplraal fgafglyaaa araagpasep aaaappaaas aasaastsqv rhaaiaaark  61 dapaldpitv tatrtasaas rtaasvsvit dsdleeqqad nikdalryep gvtvrrtayr  121 panaalgggr dgdssinirg legnrvllme dgirlpsafs fgpleagrgd yadldtlari  181 eilrgpasal ygsdgltgav nfitkdpsdl lsihrkktyf sfrpsydsvd rsigatvtaa  241 ggndrvqaml iasgrrghel dthgddnsas trrtranpqd vytesllgkl titptardti  301 klaaetvrrr idtnvlsain ppttlgltan drlernrfsi dydlrdaaar gfqtahvqfy  361 yqestqdqda fetrggrlqs rsrsnhyser alggsafaes gfatgplahk llygvdgsid  421 rikslregtv aspgesfpnk afpdtdyslf gafvqdqigf gkllvtpglr fdayrlspss  481 gdplftgktv sssdhelspr vamlyeaspa lipyaqyahg frtptpdqvn nsfsnpiygy  541 tsignpnlkp etsdtleagl rgtlgtgygp lrysvaafag ryrnfisqrt vggsgrpndp  601 lvfqyvnfan arihgfegra ewvmpngftl ktamaftkgt tqdngaasep ldtvnpfsav  661 fgvryepser wfaqadllwq agkrgrdvss aacqkktcft ppssfvvdlr ggyrfnkhvs  721 aylgihnlfd rkywnwsdvr giaadsnvld aytapgrsva vsmkvdf  606 88 124384342 YP_001028663 124 1 atgagccggg cgccgcatgc accgtcgcgc cgtcgcgcgt tcgggcgcgg gttcgcggcg  61 gctgctcgcg ccgctcacgg cgcgcctgtc gcgcatggcg cgcacgcgtg cttcgccgcc  121 gcgtcgcgtc gcgcgacggc cgtcgagcgc tgcgtcaccg cgctcgcgtg cgcggtgacg  181 gcgtccggcg cgctcgccgc cgaggcggaa ccggccgacg ccgcgcgcgg cccgcatcgc  241 gaactgccga ccgtgcgcgt gacgagcgac ggggtgcacg cgtcgccgct gtctacgccg  301 ctcgcggccg gctcgcgcct gaagctcgcg agcctcgaca cgcccgcgag cgtcgaggtg  361 atcacctccg cgcagatcgc cgcgcgcggc gatcgcacga tcgtcgacgc cgtcacgcgc  421 gcgacgggct tcagcacggc cgcggcgccc ggcaacggcg gcacggcgct cagcgtgcgc  481 ggcttcgccg gccaggaatc ggtgacgacg cttgtcgacg gcgtgcgcct ctaccccggc  541 gcgggcaccg tcacgttccc gttctcgacg tggtccgccg agcgcatcga ggtgctgcgc  601 gggccggcgt cggtgctgta cggcgaaggg gcgatcggcg gcgtcgccaa cgtcgtgacc  661 cggcgcccgc gccgcgaacg ctcgacgacg ctgcaggcgg gcatcggcac gcaaggcgaa  721 aagcgcgtgg cgctcgatac gacgggcgcg ctcggcgcgc gtctttccta tcgtttccat  781 ctgagcgacg agcgcacgcg cggcttcgtc gagcgcggcg acgcgcacgt gacggccgtc  841 ggcggcgcgc tgaagctcga tgtcgattcg cgtctgtcga tcacgctcga ttacgactac  901 ggccgccaga agccggcgac ctatttcggc gtgccggcga cgaacggcgt gctcgagcgc  961 gcgctgcgcg agcgcaacta caacgtcggc gacgcgacga tcgcatacca cgacacctgg  1021 acgcggcttg ccgcgaccta tcgcgcgggc aacggcgtga cgctcgatgc ccagctctac  1081 tatctcgcga cgcgccgcca ttggcgcaac gcggaatcgt atgcgctcga cccggcggcg  1141 cgcaccgtcg cgcgcagcga ctatctcgag atcttccatc gcgagcggca gttcggcgag  1201 cgcttcaccg cacgcatcga ctcgcgcgtg ttcggccgcg cgaaccggct cgtgctcggc  1261 gccgagttca accagatcgc gttcgacggc gcgaacaacg cgccgtatcg cggcgaatcg  1321 accgtcgccg ccgccggctt cgatcccggc gcgttcgcga gccccgatcc gacgctgccg  1381 cgctttcgca cgcgcacgca tcaggccgcg gcgttcatcg agaaccggct cgaggtcctg  1441 ccgcggctcg cgtgggtgag cgggctgcgt tacgaccatc tgtcgtttca tcgcgacgat  1501 ctgatcgcgg gcggcgcgtt cgacaagacg ttcgcgcaca ccggctggcg cagcgggctc  1561 gtctacgaga tcgcgccggg cctcgccgcg tacgcgcagt acacgacggg tgcgcagggc  1621 gtgggctcgc tcgtcacgct gtcggcgtcg caggcgaact acacgctcgc gaccgggcgt  1681 caatgggaag cgggcatcaa gcacgagatc gacggcgctc gcgcgtactg gacgctcgcc  1741 gtctacgaca tcgtcaagcg cggcctcgtc agcgtcgatc cgctgaatcc ggcgcgcgcg  1801 cagcagatcg gccgccagtc gtcgcgcggc gtcgagctcg cgggcggcgt gcggctgccg  1861 ggcggtgtga cggtcgacgc aaacgcggcg ttgctgcgcg cgcgctacga cgcgttcgac  1921 cagcgcgtcg gcgacacggt cgtgcagcgc gccggcaacg tgccgcacga catcgcgcag  1981 cagagcgcga acctgtggat cggctgggcg ttcgcgcccg gctggcgcgc gaacgcgggc  2041 ctgcgctacg tcggtccccg ctacggcgac gacgcgaacc gcgtgccggt gccttcttac  2101 acggtgttcg acgcgtcgct cgcgtggcgg gcgacgcgcg acgccgacct cgtgctctat  2161 gcgcgcaacc tcgcgaaccg cacgtacgcg gcgtcgacgt tgaacggcgg cgcgcaatgg  2221 ctgctcggcc cgtcgcgctc ggccgagctc gtcgcgacgc tgcgcttcta g  104 1 msraphapsr rrafgrgfaa aaraahgapv ahgahacfaa asrrataver cvtalacavt  61 asgalaaeae padaargphr elptvrvtsd gvhasplstp laagsrlkla sldtpasvev  121 itsaqiaarg drtivdavtr atgfstaaap gnggtalsvr gfagqesvtt lvdgvrlypg  181 agtvtfpfst wsaerievlr gpasvlygeg aiggvanvvt rrprrerstt lqagigtqge  241 krvaldttga lgarlsyrfh lsdertrgfv ergdahvtav ggalkldvds rlsitldydy  301 grqkpatyfg vpatngvler alrernynvg datiayhdtw trlaatyrag ngvtldaqly  361 ylatrrhwrn aesyaldpaa rtvarsdyle ifhrerqfge rftaridsrv fgranrlvlg  421 aefnqiafdg annapyrges tvaaagfdpg afaspdptlp rfrtrthqaa afienrlevl  481 prlawvsglr ydhlsfhrdd liaggafdkt fahtgwrsgl vyeiapglaa yaqyttgaqg  541 vgslvtlsas qanytlatgr qweagikhei dgaraywtla vydivkrglv svdpinpara  601 qqigrqssrg velaggvrlp ggvtvdanaa llrarydafd qrvgdtvvqr agnvphdiaq  661 qsanlwigwa fapgwranag lryvgprygd danrvpvpsy tvfdaslawr atrdadlvly  721 arnlanrtya astlnggaqw llgpsrsael vatlrf  607 81 124383128 YP_001025604 125 1 atgacatcca cattcctgcg tcacgcgccc gccgcgcgtg aaggccacgc gcgccgccgg  61 cggcgcgcga tcacgcttac cgttcccgcg ctcgccgcgg gcgcctttca cctcgcgccg  121 gccgtcgcgc agacgagcga ggccgtgcac ggccacggca cgctcggcgc gtccggcgta  181 gcgagccgcg ccgagaccga cgcggcgagc gccaaatcgg acggcgcggt tcgcgaagcg  241 gcgagccgca cggcaaccgg cgccgcgccg gacgccacga cgctgccgac gatcgaaatc  301 gtcgcggcgc ccgaatcgac gccgctcgtc gtcgtcaccg atccgaagac gccgcgccag  361 ccgctgcccg cgagcgacgg cgccgattat ctgaagacga ttcccggctt cgcgtcgatc  421 cgcagcggcg gcacgaacgg cgacccggtg ctgcgcggga tgttcggctc gcggctgaac  481 attctcgcga acggcatgcc gacgctcggt gcgtgtcccg gccggatgga cgcgccgacg  541 tcgtacatcg cgtccgagag ctacgacaag gtgacgctcg tcaaggggcc gcagaccgtg  601 ctgtacgggc cgagcgcatc ggcgggcacg gtgctgttcg agcgcgtgac gccgcgcttc  661 aagacgccgg gcatgcgctt tgacggcagc gtcgtcggcg gctcgttcgg gcgcaacgat  721 cagaacgtcg acgtgacggc cggcacgccc gacttctacg ggcgcgtgag cgcgaaccat  781 gcgcactcgc aggactacga ggacggcaac ggccgcacgg tgccgtcgca atgggacaag  841 tggaacgcgg atgcggcgct cggctggacg cccgacgaca acacgcggct cgagctgacg  901 gcaggcacgg gcgacggcta cgcgcgctat gcgggccgcg gaatggacgg cgcgcatttc  961 cggcgcgaga cgttcggtct gaagttcgac aagaagcaca tcggcgacgt gctcgatcgc  1021 atcgaggcgc aggtcttcta caacgaagcc gatcacgtga tggacaacta cacgttgcgg  1081 atgcccgatc cgacgagcag catgccgatg cgcatggcct ccgaagtgcg ccgccgcacg  1141 ctcggcgcgc gcgtcgcggc gacgctgcgc ttgaccgacg cgttcaagct cgtgacgggc  1201 gtcgatgcgc agtcgaaccg cctcgactcg cgctcggcga tggggatgca gaactacggc  1261 gacaagccgt ggaatccgca ggcgaacatg tggaacgcgg gcgcgttcgg cgagctgacc  1321 tggtatgcga gcgatgcgtc gcgcgtgatc ggcggcgcgc ggatcgacta tgcggccgcg  1381 cgcgacaagc gcgcgacgac gggcggcatg aagatgagca tgcgcaatcc gacgttcgac  1441 gatctccgct cgcgcgtgct gccgagcggc ttcgtgcgct acgagcgtga tctcgcgtcg  1501 ctgcccgtca cgtggtacgc gggcatcggc catgcgcagc gcttccctga ttactgggag  1561 ctgttctccg ccaagcgcgg cccgaacggt tcgatcaacg cgttctccgc gatcaagccc  1621 gagaagacga cgcagctcga catcggcgcg cagtacaaga gcgacaagct cgacgcctgg  1681 gtgtccgcct atgcgggcta cgtgcaggac ttcatcctgt tcgactatgc gacgggcccg  1741 atgggacaga tcacgcgggc gacgaacgtc aacgcgcaga tcatgggcgg tgaggtgggc  1801 gcgtcgtggc gtccgctcgc gccgtggcgc ttcgaagggt cgctcgcgta tgcgtggggg  1861 cgcaacgtgc aaagcggtgc gccgctgccg cagatgccgc cgctcgaggc acgcttcggc  1921 gtcgagtaca ctcgcgggcc gtggtcggcg ggcgggctgt ggcgggtcgt tgcgccgcag  1981 catcgctacg cgctgaacga gggcaacgtc gtcggcaagg acttcggtcc gagcgccggt  2041 ttcggcgtgc tgtcgctgca cgcgcagtac aacgtgagca agacggtgca gatctcggtc  2101 ggcgtcgaca acgtgctcga caagacttat gcggagcacc tgaacctcgc gggcaacgcc  2161 ggtttcggct atccggcgaa tctgcctgtc accgaacccg gccgcaccgc gtgggttcgt  2221 ttgagcacca agctctga  105 1 mtstflrhap aaregharrr rraitltvpa laagafhlap avaqtseavh ghgtlgasgv  61 asraetdaas aksdgavrea asrtatgaap dattlptiei vaapestplv vvtdpktprq  121 plpasdgady lktipgfasi rsggtngdpv lrgmfgsrln ilangmptlg acpgrmdapt  181 syiasesydk vtlvkgpqtv lygpsasagt vlfervtprf ktpgmrfdgs vvggsfgrnd  241 qnvdvtagtp dfygrvsanh ahsqdyedgn grtvpsqwdk wnadaalgwt pddntrlelt  301 agtgdgyary agrgmdgahf rretfglkfd kkhigdvldr ieaqvfynea dhvmdnytlr  361 mpdptssmpm rmasevrrrt lgarvaatlr ltdafklvtg vdaqsnrlds rsamgmqnyg  421 dkpwnpqanm wnagafgelt wyasdasrvi ggaridyaaa rdkrattggm kmsmrnptfd  481 dlrsrvlpsg fvryerdlas lpvtwyagig haqrfpdywe lfsakrgpng sinafsaikp  541 ekttqldiga qyksdkldaw vsayagyvqd filfdyatgp mgqitratnv naqimggevg  601 aswrplapwr fegslayawg rnvqsgaplp qmpplearfg veytrgpwsa gglwrvvapq  661 hryalnegnv vgkdfgpsag fgvlslhaqy nvsktvqisv gvdnvldkty aehlnlagna  721 gfgypanlpv tepgrtawvr lstkl  608 81 124381644 YP_001024240 126 1 tcagaactgc agcgtcgtca gcagcgacac ctgccgcgcg tcgcccaccg acacgaagaa  61 ccggttcacg ctcgacgggt aatacgtgcg gttgaacaga ttcttcacgt tgagctggaa  121 ctgcagcttc tgcttgccga gccgcgtgtc gtaggtcgcg aacgcgtcgg cggtcgcgta  181 cgccggcagc gtgaagctgt tggccgaatc gcccgggcgc gcgccgacgt agcgccccgc  241 cgcgccgatg cgcaggtcgt cgccgccgag caccgtgccc acgtcgtaga ccgccgcgag  301 cgaggccgtg tgccgcgcga cgttccacag ccggttgtcc gcgtacagcg gatcttcggt  361 cgtcttcgcg tcgatgtacg cgtagctcgc gatcacgttc caacgcgcac cgatccgccc  421 cgacacgtcg agctcgatcc cgcgcgagcg cgcgcggccg gacgtgcgcc acgcggtctg  481 gttcgtcgcg tcgtcgtatt gcgagacgag cacgtgcttc ttgtcgatgt cgaagaacgc  541 gagcgtaccg gcgaggccgc ccggcatgtc gagcttcgcg ccgagctccc acgacgcgcc  601 ttcctcgggc gcggtcgagc cgtcgatcac gtagccgccc gccatcggcg cgatcttcga  661 ggtcggcttc agcgactgcg tgtagctgcc gtacagcgac agcgcgtcgt tccacttgta  721 gacgatgccc gcgcgcggca gccacttcgt gccgctcagg ttcgtgttga cctggaacgg  781 ccggccgcgc ccggcaagct ggctgtagcg gacccagcgc gcgccgccga cgagcatcca  841 ccgctcgctc agatggatgc tgtcctggaa gaacagcgag gcggtgtgca gcgtgtcgct  901 ctggtcgctg tcggaggccg atacgctcgt cgacggcggg acgagcccgt acgtcgggtt  961 cagatagctg aacggcgtct tgatcggctg gcgcagcatg tccgcgcggt agacctgccg  1021 gtattcgccg tcgacgccga actgcacgtc gtgccgcatc ccggcgagcg tcacgcggcc  1081 gtcgacgtac gcgatgccgt agctgtcggt gctgcgcgaa ccgtgcgtcg cgtcgttgct  1141 gcgcgtgagc gtgcccttga gcggatcgac cgccgtgatg cgaatctggt tcgcatcgta  1201 ggtctcccgg ttgtagctgt agccgacgtg caccttccag tccggcgcga gctgatgatc  1261 gatcgcgagc tgcgcgagat tcgattcgcc gcgcatgtcg ttgaacggct cgtcgagccg  1321 gcggcgcgcc gggatcgcga gcggcgcgtt cgtgcgcgga tcgagcgcgg tgccgcgatc  1381 gaacggcatc aggaagcgcc ggtactcgta cgacagcacc acctgcgtat cgcgcccgta  1441 ccacgcgagc gaaggcgcga cgagcgtctc gcggtgctcg ccgtagttgc gccaatactg  1501 ttcgttcgtc tgatcgacga tcagccggta tgcaacgcgc gactcgccga tcgcgcccgt  1561 cgagtcgaac gtgagctcgc cgccgttcct gccgccgccg tacgtcgagc cgagcgccga  1621 gatcgcgtgg cggcgcgcga gctgcggctg cttcgtgacg acgttgatca cgccgcccgg  1681 gtccatgatt ccgtacagca gcgaggccgg gcccttcagc acttcgacgc tgtccgtcgt  1741 cgcgttcagc gagcggccct gcacgatcgg cattccgttg cgcatcaccg agccgtcgcg  1801 gttgtcgccg aagccgcgct tcatcaccgt gtcctgcgtg ctgccgagcg tgttgccctg  1861 cgtgatgccg ctcacgttcg caagcgcgtc gtcgagattg cgcgggcgct ggtcgcgcag  1921 cacctgctgc gcgacgatgg cgaccgcttg cggcacctcg gcgagcggcg cgtcgctgcg  1981 cagccccgcc gcctcgcgcg gcggccggta gctgtccgcg tgcgggccgc tcgcgcgcac  2041 ggcgacggtg ggcagcgtcg tgtcggccgc gagcgcggca tcggcgccgg cggcggccgg  2101 cccgggcagc ctggcgagcg tgtagccgcc gctcggctgc cggagcgcga cgagccccgt  2161 gcccgtcagc agccggtcga gcgccgcggc cggatccgcg cggccgtgca gcccggggct  2221 tcgcaggccc gtggtcagct cgcccgggaa cgacagcagg atgccggcct cgcggccgaa  2281 gcgggtcagc gccgcgtcga gcgggcccgc cggaatgtcg tacccgcgcg tcgcggcggc  2341 cggctcggcc gcgcccgata cgcccggcgc ggcgcacgcg gagaacagcg cggcggcggc  2401 cacgcgcgcg acgacgcggc gcggggcgaa ttgcggcatg cacggcgcgt gcaacgtgtg  2461 cgacgcgtgc gatgtgtgcg atgtggcttg ccgcgcgcat cgcggcgcgg ccgcgtcgcg  2521 cggccggcct gcgccgagcc gcgcggcggg ctgagccgcc tcgggcaaac cggccggcgc  2581 gtgcgccgta tccgcgcaca cgccggcgtg cgcgcggcgg gggcggcgat tgcagattga  2641 atccatgatc gatgactcgt tgacgatggg tggaatgcag ccttcatttc ccatgtcacg  2701 cgagcatcga aaacagctca ggcccggcga aaaaatttcg atgaggcatg cggcgcgcgt  2761 gcgcaa  106 1 mrtraaclie ifspglscfr csrdmgnegc ippivnessi mdsicnrrpr rahagvcadt  61 ahapaglpea aqpaarlgag rprdaaaprc arqatshtsh ashtlhapcm pqfaprrvva  121 rvaaaalfsa caapgvsgaa epaaatrgyd ipagpldaal trfgreagil lsfpgelttg  181 lrspglhgra dpaaaldrll tgtglvalrq psggytlarl pgpaaagada alaadttlpt  241 vavrasgpha dsyrppreaa glrsdaplae vpqavaivaq qvlrdqrprn lddalanvsg  301 itqgntlgst qdtvmkrgfg dnrdgsvmrn gmpivqgrsl nattdsvevl kgpasllygi  361 mdpggvinvv tkqpqlarrh aisalgstyg ggrnggeltf dstgaigesr vayrlivdqt  421 neqywrnyge hretlvapsl awygrdtqvv lsyeyrrflm pfdrgtaldp rtnaplaipa  481 rrrldepfnd mrgesnlaql aidhqlapdw kvhvgysynr etydanqiri tavdplkgtl 541 trsndathgs rstdsygiay vdgrvtlagm rhdvqfgvdg eyrqvyradm lrqpiktpfs  601 ylnptyglvp pstsvsasds dqsdtlhtas lffqdsihls erwmlvggar wvrysqlagr  661 grpfqvntnl sgtkwlprag ivykwndals lygsytqslk ptskiapmag gyvidgstap  721 eegaswelga kldmpgglag tlaffdidkk hvlvsqydda tnqtawrtsg rarsrgield  781 vsgrigarwn viasyayida kttedplyad nrlwnvarht aslaavydvg tvlggddlri  841 gaagryvgar pgdsansftl payatadafa tydtrlgkqk lqfqlnvknl fnrtyypssv  901 nrffvsvgda rqvsllttlq f  609 27 124385832 YP_001028928 127 1 ttactgcgcc gcttgcggcg cgcgcaggta gatctcgacg cggcggtttt gcgcgcggcc  61 ggcttccgtt gcgttgtcgg cgatcgggtt cgacgcgccc atgccttgcg cggacaggcg  121 gttcgccgcg acgccgcgct gcgcgagcgc gttcacgacg ctttgcgcgc gattttgcga  181 cagcgtttga ttgtgcgcgg ccgagcccgt gctgtcggtg tagccgacga ccgacgcggt  241 gatctgcggg ttctggttca gcgtcgtcgc gaggtcgttc agcagcggcg tgaacgccgg  301 cgtgatcgcg tactggtcgg tcgcgaacgt gaccgagctg ggcacgttca gcttgagcga  361 gccgtccggc tgttcggtga cctgcgtgcc cgtttgctgc gccgacggcg cgagcttgtt  421 cttgatcgct tgccagttgt aacccgtcac gccgccgacg agcgcgccga cgccggcgcc  481 gatcgccgcg cccttgccgc cgccagcgag cgcgccgatg cccgcgccga gcgcggcgcc  541 cgtgccggtg ccgacagccg tgttggtgcc ttgctgggtt gcgcaacccg ccagcagggc  601 gccggcgaga gcgaaaacgg acaagcgcgt cgcgattttg gtattcat  107 1 mntkiatrls vfalagalla gcatqqgtnt avgtgtgaal gagigalagg gkgaaigagv  61 galvggvtgy nwqaiknkla psaqqtgtqv teqpdgslkl nvpssvtfat dqyaitpaft  121 pllndlattl nqnpqitasv vgytdstgsa ahnqtlsqnr aqsvvnalaq rgvaanrlsa  181 qgmgasnpia dnateagraq nrrveiylra pqaaq  610 19 124383503 YP_001028612 128 1 ttactgttga tagacgaggt ccgcacggcg gttctgcgcc cacgacgctt cgtcgtggcc  61 gagcgcaacc ggcttttcct tgccgagact cacggcttcc atctgcgcat ccccgacgcc  121 gagcagcgac agcgcgcggc gcacggcttc cgcacgcttt tggccgagcg cgaggttgta  181 ctcgctcgtg ccgcgctcgt cggtgttgcc ctggatcagg atgtggcgtt gcggatggct  241 cttcaggtat tgcgcgtgtt gctgcagcag cgcctggtat tggtcctgca ccgagtagct  301 gtcgaaatcg aagtacacgc tgcgcttcgc gagcgggctg ttcggatcgt tcagcggatc  361 gacggtcacc tgcgcgacgt tttcgggatt cggttgcgtg ctgaccgcgt cgccctggtt  421 cgcgtgctcg tcgagcttca cgcccgactt gcatgccgcg agcgcgccga tcatcagcat  481 cgcgaacgcc agacgaagtt tcttcgacat at  108 1 mmskklrlaf amlmigalaa cksgvkldeh anqgdavstq pnpenvaqvt vdplndpnsp  61 lakrsvyfdf dsysvqdqyq allqqhaqyl kshpqrhili qgntdergts eynlalgqkr  121 aeavrralsl lgvgdaqmea vslgkekpva lghdeaswaq nrradlvyqq  611 24 124386037 YP_001026945 129 1 atgaacggca cgaacgagct ctgctggcgc gatgcgttct ggacgccggc caccgccaac  61 gccaagtgcg atggcgcact ggtcgcccag gcaccggcac cggcgccggt cgcaccggtt  121 gctccggcca tcacgagcca gaagattacg taccaagccg atacgctgtt cgacttcgac  181 aaggccgtcc tgaagccggc cggcaagcag aagcttgacg aactggccgc gaagatccag  241 ggcatgaacg tcgaagtggt cgtggccacg ggctacacgg accgcatcgg ttcggacaag  301 tacaacgacc gtctgtcgct gcgccgcgcg caagccgtca agtcgtacct cgtcagcaag  361 ggtgtcccgg cgaacaaggt ctacacggaa ggcaagggca agcgcaaccc ggtcacgggc  421 aacacctgca agcagaagaa ccgcaagcag ctcatcgcct gcctcgcacc ggaccgccgc  481 gtggaagtcg aagtggtcgg cacgcaggaa gtgcagaaga cgaccgttcc ggcgcagtaa  109 1 mngtnelcwr dafwtpatan akcdgalvaq apapapvapv apaitsqkit yqadtlfdfd  61 kavlkpagkq kldelaakiq gmnvevvvat gytdrigsdk yndrlslrra qavksylvsk  121 gvpankvyte gkgkrnpvtg ntckqknrkq liaclapdrr vevevvgtqe vqkttvpaq  613 44 124383016 YP_001024349 130 1 tcagaacttg tgacggatcg ccgcgcgaac cgcgaactgg ttcgccgacg ccgaggggcc  61 gtccgtcccg acgacgtagc cgccatccgc gatcgtgccc gtcttgtcgc ccgcgacctt  121 ctggtacgcg ccctgcagat agacgtcggt gcgcttcgac aggttgtagt ccgccatcag  181 gccgaccgtg tggtacttcg gcttgacgct gcccgcggcc gcatcgaact tgccgtccgt  241 gtacacgtac tgcgcgccga tgaagagcgc cggcgtcagc tggtatttgc cgttgatttc  301 gaagttctgg aacttcgtcg cgctcaggcc gaggcccgcg aacgtcgccg tcggcagata  361 gaccgtcgag accgggttct tcacgtcggt cttcgtgtag acgaaaccga ccgtcgccgg  421 gccgaacgtg tagttgacgc cgccgccgaa gatgcgcagg cggtccgccg tgaagttcgc  481 gtcgttgtcg gcgatcgcgc cgcccgccgt cttgcccggg ttgttcgcct gcaggtacgc  541 ggccgcgagt tgcaggccgc ccagcgtgta cgcggcgccg atgctgtact ggcggttgtt  601 cgagaagccc gtcgcgttgc tgaagctgta cgtgccgccg acttgcagac cgttccagtc  661 cgggctcgcg tacttgaccg tgttgttcac gcggaacgag ttgtccgtgt tgtcgttgtc  721 gaacggatgc gagaacagcg tgccgcccca gttgccgttc gcggtcaacg gcgcgaggta  781 gtcgacgagc gaatcgtact ggcggccgag cgtgagcgag ccgtattgcg cgtgcgacag  841 gccgacgaac gcctggcggc cgaacatccg gccgccctgg ccgagccggc cgttgttcac  901 gtcgaaaccg ttttcgagcg tgaagatggc tttcaggccg ccgcccaggt cttccgagcc  961 gcgcaggccc cagcggctgc cctgcacgaa accgctcgcg agctggtagt tgctcttgcc  1021 gacgccgttg acgttcacgt tgctcgtgta gttgaagcct tcgtcgatca cgccgtacag  1081 cgtgacgctg ctttgggcga aaaccggcgc ggcgaaggcc agcaatgcgg ctgcggaaat  1141 gacgtgcttt ttcat  110 1 mkkhvisaaa llafaapvfa qssvtlygvi degfnytsnv nvngvgksny qlasgfvqgs  61 rwglrgsedl ggglkaiftl engfdvnngr lgqggrmfgr qafvglshaq ygsltlgrqy  121 dslvdylapl tangnwggtl fshpfdndnt dnsfrvnntv kyaspdwngl qvggtysfsn  181 atgfsnnrqy sigaaytlgg lqlaaaylqa nnpgktagga iadndanfta drlrifgggv  241 nytfgpatvg fvytktdvkn pvstvylpta tfaglglsat kfqnfeingk yqltpalfig  301 aqyvytdgkf daaagsvkpk yhtvglmady nlskrtdvyl qgayqkvagd ktgtiadggy  361 vvgtdgpsas anqfavraai rhkf  614 39 124383230 YP_001024431 131 1 ttagaagcgg tgacgcagac caaccgtcgc tgcggtttgg ttgatcgacg tgctgaacgg  61 cgtgctgagg tcgccgttgt agatcgacgc attcgcgttc ttcgacgaac gctggtacac  121 ggcttgtgcg tacacgtcgg tgcgcttcga cagcgcgtag tcggcctgga cgccaacttg  181 gttccagtga gtgctttcgc cgttcgcctt cgcgttcgtg tacgtgtacg tgtaagcaac  241 acccagaccc agagccggcg tcaggttgta cttcacgttt gcttcgtagt tgtcggcacg  301 gatggtcggc gcgccgtttg ccaggttgtc gagacgcgat tgcgtccaga gcaggccgcc  361 ttgcagcggg ccgtaagcgt agcttgcacc ggcgccgtac acgcgcgagc ggccttggat  421 gcttgctgcg ttcgtgccgc cgatgttgaa gccggtcagc ggatccgttg cgcccgtggt  481 gttcgcgcca gcgttgttag cttgcgagta cgccgcaccg accttcaggc cttggaactg  541 gtacgaagcg cccgcgctgt atgcacggtt gttcgcgaat tgcgagttgt tcgagaacga  601 gtacgtgccg ccgaattgca ggccggcgta gttcgcgctc gtgaacttga ccgtgttgtt  661 caccgcgacg tcgccgttcg tgttcaggcg gtcgttgttg agcgggtgcg cgaagtacgt  721 gccgccccag gtgcccgttg ccgacagcgg cgacaggtag tcttgggttg cgtcgtactg  781 acggcccagc gtgacggtgc cgtagttgct cgacagaccg acgaacgctt gacggttgaa  841 catgccgcca ccgttgttga agcggccgtt accgatgttg aagccgcttt ccaacgtgaa  901 gatcgccttc aggccgccac ccaggtcttc cgagccgcgc agaccgaaac ggctttggtc  961 gacgccggcg ccgaccgacc acagcgactt gcccgagccc gacggcgtcg cgacgttgct  1021 ttggtacgtg atgcccgcgt cgagcacacc gtacagcgtg acgctgcttt gcgcgtgcgc  1081 gacggttgcg aacgatgcag caactgctgc aacaatcaga gtcttgttca t  111 1 mnktlivaav aasfatvaha qssvtlygvl dagityqsnv atpsgsgksl wsvgagvdqs  61 rfglrgsedl ggglkaiftl esgfnigngr fnngggmfnr qafvglssny gtvtlgrqyd  121 atqdylspls atgtwggtyf ahplnndrln tngdvavnnt vkftsanyag lqfggtysfs  181 nnsqfannra ysagasyqfq glkvgaaysq annaganttg atdpltgfni ggtnaasiqg  241 rsrvygagas yaygplqggl lwtqsrldnl angaptirad nyeanykynl tpalglgvay  301 tytytnakan gesthwnqvg vqadyalskr tdvyaqavyq rssknanasi yngdlstpfs  361 tsinqtaatv glrhrf  615 55 124381981 YP_001025679 132 1 ttagaacttg tggcgcaggc ccagagccac catctcttgc gacttcgtac cgttcacgcc  61 gtacgagccg atcgatgcct gcgccgtttg cgtcgtcgtc gcgtcgacgc gctgcgtgcc  121 gctcgcgtgc tggtacgcac cggcgaggta gacgtccgtg cgcttggaca gcgcgtagtc  181 cgcgccgatc gacacttggt ggtacttcgc gtccgtgtcg ccgctcgcct tcgtgtatgc  241 gtagccgagg ccgagcagca gcgccggcgt ggcctggtac ctcacgaagc cgcggcccgt  301 gttgtacttc tcggtcgacg agaagcccga gaagccgtcc ggcttgtatt gcgcgttgct  361 gtagccgagg ccgaccgtga ccgggccgaa cacgtactgg ccggccacct gtgcgatgcc  421 gatcgacttc gcgctcgtgt agccgctgtt gatcgcgccg tcgaagatcg cgtcggacga  481 gccgctccac gtcgagcgcg agccggccgt cggcgacggg ttcgacgtgt agaagtagcc  541 ggccgcgacg ccgatcgggc cgttgttgta tgcggcggcg gccgaccacg tctggccttg  601 gcccggcgag cccgcgatgc cgctgaagcc gtacagggct tcgaactgga agccggcgaa  661 cacgggcgac gtgtacttga ccgtgttgtt cacgcgcagg ctgttgtcgt tgttgtcgac  721 gtcacccggg gtggcgaaca ggctgccgaa gtagttgtcg gccgtcaccg gttgcacgag  781 gtcgacgagc ggatcgtact gacggccgag cgtcagcgtg ccgtactggt catgctgcag  841 accgacgaac gcctggcggc cgaacatgcg cttgccttgg ccgagcgtgc cgtcgttcga  901 gttgaagcca ttttccaact ggaagatcgc cttcaggccg gcgccgagat cctcggtgcc  961 cttcaggccc cagcggctgc cttgcaggtt gccgctgccc atcagccagg cgttgttggc  1021 cttgccgtcg ttgccgtgga cataagtgat cgacgtatca atcacgccgt acagcgtgac  1081 gctgctttga gcctgagctg caccggctgc gcccagcagc gcgagcgaga gggtagacag  1141 agcgagtttc ttcat  112 1 mkklalstls lallgaagaa qaqssvtlyg vidtsityvh gndgkannaw lmgsgnlqgs  61 rwglkgtedl gaglkaifql engfnsndgt lgqgkrmfgr qafvglqhdq ygtltlgrqy  121 dplvdlvqpv tadnyfgslf atpgdvdnnd nslrvnntvk ytspvfagfq fealygfsgi  181 agspgqgqtw saaaaynngp igvaagyfyt snpsptagsr stwsgssdai fdgainsgyt  241 saksigiaqv agqyvfgpvt vglgysnaqy kpdgfsgfss tekyntgrgf vryqatpall  301 lglgyaytka sgdtdakyhq vsigadyals krtdvylaga yqhasgtqrv datttqtaqa  361 sigsygvngt ksqemvalgl rhkf  617 55 124385469 YP_001028406 133 1 atgaagcgaa aacatgcttt gactgcactc gcagtcgcgc tgctcgccgc gggctgcacg  61 ctcgcgccgc gctacgagcg tccggccgcg ccggtgtcgg gcgcgttccc cgccgacggc  121 gtctatgccg cgcagccggg cgccgcgccc ggcgcgcgca gcgcgaacgg ccaggcggcc  181 gtcgatatcg gctggcgcga gttcttcgtc gatccgcgcc tgcagcggct gatcgagatc  241 gcgctgaaga acaaccgcga cctgcgcgtg tcggtgctca acgtcgaagc gtcgcgcgcg  301 cagtatcaga tcacgcgcgc ggggctcttc ccgacgttga gcggcaccgg cacgggcacg  361 atccagcgca cgccggccgg cgtgtcgatc accggccagc cgctcatctc gcggacctac  421 aacgtcggcg tctccgcgtc gtgggagctc gacctgttcg gccgcgtgca gagcctgaag  481 gaccaggcgc tcgcgcaata cttcgccacc gcgcaggcgc gcaaggccgc ggagatctcg  541 ctcgtcgcga gcgtcgccga tcagtacctg acgctgctgt cgaccgacga tctgctgcag  601 gtcacgcaga acacgctgaa gtcggcgcgc gcgtcctacg atctgacgaa gctgcagttc  661 gacaacggca ccggctcgga gctcgacctg tgccaggcgc agacggtggt cgagaccgcg  721 ctcgcgagcc agcaggcgca ggcgcgcgcc cgcgcgcagg cgctcaacgc gctcgtgctg  781 ctgatcggcg agccgctgcc cgacgatctg ccggccgacc tgccactcaa tgcgcagaac  841 ctgctcaccg acattccggc cgggctgccg tccgatctgc tcacgcggcg gcccgacatc  901 atgcaagccg aggagacgct gcgcgcggcg aacgcgaaca tcggcgcggc gcgcgcggcg  961 ttcttcccga agatctcgct caccagcgcg ttcggcaccg cgagcacgac gctcggcggc  1021 ctgttcaagg cgggcacggc ggcgtggtcg ttcgcgccga acatcgcgct gccgatcttc  1081 gagggcgggc agaacatcgc gaacctcgat ctcgcgcacg tgcagaagcg catcgagatc  1141 gcgaactacg agaaggcgat ccagagcgcg tttcgcgagg tgtcggacgg gcttgccgcg  1201 cgcggcacgt acgatcagca gatcgcggcg ctcgagcgca acgagcacgc gcagcagcgc  1261 cgctacgatc tgtcggacct gcgctacagg aacggcgtcg acagctatct gtcggtgctg  1321 accgcgcaga cggacctgta ttcggcgcag cagcagttga tcagcgcgcg gctcgcgcgc  1381 tggacgaacc tcgtggacct gtatcgcgcg ctgggcggcg ggtggctcga gcgcgcgggc  1441 gagacgccgc gcccggcgga cgcgcccgtc gattacggca aggcggcgcc cgcgccggcg  1501 gcggccgtgc cggctgcgtc ggccagcgcg ccggcggcgg gctga  113 1 mkrkhaltal avallaagct lapryerpaa pvsgafpadg vyaaqpgaap garsangqaa  61 vdigwreffv dprlqrliei alknnrdlrv svlnveasra qyqitraglf ptlsgtgtgt  121 iqrtpagvsi tgqplisrty nvgvsaswel dlfgrvqslk dqalaqyfat aqarkaaeis  181 lvasvadqyl tllstddllq vtqntlksar asydltklqf dngtgseldl cqaqtvveta  241 lasqqaqara raqalnalvl ligeplpddl padlpinaqn lltdipaglp sdlltrrpdi  301 mqaeetlraa nanigaaraa ffpkisltsa fgtasttlgg lfkagtaaws fapnialpif  361 eggqnianld lahvqkriei anyekaiqsa frevsdglaa rgtydqqiaa lernehaqqr  421 rydlsdlryr ngvdsylsvl taqtdlysaq qqlisarlar wtnlvdlyra lgggwlerag  481 etprpadapv dygkaapapa aavpaasasa paag  618 19 124381380 YP_001024890 134 1 atgcaaggcg acaagaaagt catcgaatat ctgaacgcgc agttgaaaaa cgaactgacc  61 gcgatcaatc aatatttcct gcatgcgcgg atgtacaaac actggggcct cgaaaaactc  121 ggcaagcacg aatacgacga atcgatcggc gaaatgaagc acgcggactg gctgatcgag  181 cgcgtgttca tgctcgacgg cctgccgaac ctgcaggatc tgcacaagct gctcgtcggc  241 gaggaaaccg aggaaatcct gaaatgcgac ctgaagctcg aacaggtatc gcaggccacg  301 tgcaaggaag ccatcgctta ttgcgaatcg gtgcgtgatt acgtgtcgcg cgaaatcttc  361 gagaaaatcc tcgacgatac cgaggagcac atcgactggc tcgagacgca aatcgacctg  421 atcggcaagg tcggcattca gaactatcag cagtcgatga tgggctcgcc ggaataa  114 1 mqgdkkviey lnaqlknelt ainqyflhar mykhwglekl gkheydesig emkhadwlie  61 rvfmldglpn lqdlhkllvg eeteeilkcd lkleqvsqat ckeaiayces vrdyvsreif  121 ekilddteeh idwletqidl igkvgiqnyq qsmmgspe  619 8.5 124382608 YP_001024892 135 1 atgatcgtct gcgtgtgcaa gtccgtttcc gatcggaaga ttcgcgcatc cctcgcggaa  61 ggcgtgaaca cgttcgaaga actccagttc gaactcgggg tcgccacctg ctgcggcaag  121 tgcgaggaaa ccgtgcgcga gatcatggcg gaacagggcg tttgtgcgag ccgctgcggt  181 gccgagcgcc ccgcggccgt accggtcgcc gtcacgttct acgaacgcaa ggcggcctga  115 1 mivcvcksvs drkiraslae gvntfeelqf elgvatccgk ceetvreima eqgvcasrcg  61 aerpaavpva vtfyerkaa  620 36 124382074 YP_001024065 136 1 atgctgaatt ttctggtcaa acgactgttc gggctgctgc cgacgctcgc gtgcgtcgcg  61 gtgctggtgt tcctgttcgt ccacctgctg ccgggcgatc ccgcgcggct cgcggccggc  121 cccgaggccg acgacgcgac cgtcgcgctc gtgcgcgccg atctcggcct cgacaagccg  181 ctgccggcgc agttcgcgag cttcttcgcg cggatcgcgc acggcgactt cggcgtctcg  241 acgcgcagca agcggcccgt agcgaccgag atcggcgagc gcttcatgcc gacgctgacg  301 ctgaccgtcg tcagcatggc ctgggcgacg ctcttcggca tggcgatcgg catcgcgtcg  361 gccgtgtggc gcaaccgctg gccggaccgc atcggcatga cgctcgcggt gtcgggcatc  421 tcgtttccgg cgttcgcgct cggcatgctg ctgatggagg tgttctcggt gaagctcggc  481 tggctgcccg tcgtgccgga cggctcgtgg aagagctacg tgctgccgtc ggtgacgctc  541 ggcgcggccg tggccgccgt gatggcgcgc ttcacgcgcg cgtcgttcgt cgaggtgctg  601 aacaaggatt tcgtgcgcac cgcgcgcgcg aagggcgtgc gcgagccgat ggtcgtgctc  661 aagcactgcc tgcgcaacgc gatgattccg gtcgtcacga tgatggggct gcagttcggc  721 ttcctgctcg gcggctcgat cgtcgtcgag gcggtgttca actggccggg gctcgggcgc  781 ctgctcgtcg atgcggtgac gatgcgcgac tacccggtga tccaggcgat cgtgctgctg  841 ttctcgctgg agttcatcct gatcaacctg agcgtcgacg tgctgtacgc ggtcatcaac  901 ccgacgatcc gtttcaagtg a  116 1 mlnflvkrlf gllptlacva vlvflfvhll pgdparlaag peaddatval vradlgldkp  61 lpaqfasffa riahgdfgvs trskrpvate igerfmptlt ltvvsmawat lfgmaigias  121 avwrnrwpdr igmtlavsgi sfpafalgml lmevfsvklg wlpvvpdgsw ksyvlpsvtl  181 gaavaavmar ftrasfvevl nkdfvrtara kgvrepmvvl khclrnamip vvtmmglqfg  241 fllggsivve avfnwpglgr llvdavtmrd ypviqaivll fslefilinl svdvlyavin  301 ptirfk 622 56 124383712 YP_001026175 137 1 atgaaaacct tcccgttgtc cgcttgccgg accgccgtgg ccgtcgcggt cgccgcgctc  61 gcgctcgcgg gatgcgcgaa ctatttcggc ctcaagaacg acaaggcgat cgctcccgcg  121 gcgcagttcg aaagcgcgcg gagcctgccc gcgcagggcg gccaatggcc gtcgctcgac  181 tgggcgaacc agttcggcga tccgcaattg ccgaagctga tcgacgaggc gctcgaaggc  241 aatccgacga tcgcgcaggc gcaggcgcgc atcgcgaagg cgtcgtcgta catcgagtcg  301 tcgcgctcga cgctgctgcc gaaggccgac gcgaagtact cgtggacccg cgagctgtac  361 tcgggcaacg ctcttttccc gcccccgtac ggcggccagt ggtacagcga gaacaacgcg  421 ctcgcgagcg cgtcgtggga gctcgatctg tggggcaaga accgcgcgcg cctgaatgcg  481 gccgtgtcgc aggaaaaggc cgccgaagcc gacttgcagc aggcgcgcgt gacgctcgcg  541 acgtcggtcg cgcgcacgta caaccagctc gcgcaactgt acgcgctgcg cgacatcgcc  601 gggcgcgaga tctcgaaccg gcagacggtc gacaagatca ccgacggccg cgtcggcgcg  661 ggcctcgaca cgaacgtcga gcgccagacc gcgcgcggca acatcgcgac gagccagtcg  721 acgctgtccg atctcgacgg ccagatcacg aacgtccgct atcagctcgc cgcgctgctc  781 ggcaaaggtc cggaccgcgg gctgcagatc gccgcgcccg tgctgagcgc gggcggcgcg  841 gtcgcgctgc cggagaacct gcccgccgat ctcgtgtcgc gccgcccgga tctcgtcgcc  901 gcgcgctggc aggtcgaagc ggcgatgcac gacgtgaagg aagcgaaagc cgagttcttt  961 ccggacatca acctcgcggc gggcttcggc ttcgacgcgt tcggctgggg ccgattcctg  1021 aacttcgcga gccgccaggc gcagttcggc cccgcgatcc acctgccgat cttcgacggc  1081 ggcgcgctgc gcgcgcaatt gaagggccgc tacgcggact tcgatctgtc ggtcgcgaac  1141 tacaaccaga cgctcatcaa cgcgctgaac gacgtcgcga cgcaagtcgc gtcgattcgc  1201 tcggtcgaca cgcaaatggg cgacgcgcag cgcgcgctcg atgcgtcgac gcgcgcctac  1261 gagctcgcgg tgatccgcta caaggcgggc ctgtcgccgc agttgcaggt gctcaacgcg  1321 gacagcaacc ggctcgccgc cgagcagacg gtgacgaacc tgaagatgcg ccgccgcgac  1381 ctgcagatcg gcctcgtgaa ggcgctcggc ggcggcttcg acgcggccgg cacgcggctc  1441 gccgcgcccg cccccgcctc ggcgccggcc gcgcccgcgc agcacgcatc gaactga  117 1 mktfplsacr tavavavaal alagcanyfg lkndkaiapa aqfesarslp aqggqwpsld  61 wanqfgdpql pklidealeg nptiaqaqar iakassyies srstllpkad akyswtrely  121 sgnalfpppy ggqwysenna lasasweldl wgknrarlna aysqekaaea dlqqarvtla  181 tsvartynql aqlyalrdia greisnrqtv dkitdgrvga gldtnverqt argniatsqs  241 tlsdldgqit nvryqlaall gkgpdrglqi aapvlsagga valpenlpad lvsrrpdlva  301 arwqveaamh dvkeakaeff pdinlaagfg fdafgwgrfl nfasrqaqfg paihlpifdg  361 galraqlkgr yadfdlsvan ynqtlinaln dvatqvasir svdtqmgdaq raldastray  421 elavirykag lspqlqvlna dsnrlaaeqt vtnlkmrrrd lqiglvkalg ggfdaagtrl  481 aapapasapa apaqhasn  623 85 124383487 YP_001026988 138 1 tcacagcgac catttcaact ctccataaaa agtgcggccc ggataaggat ggaacacgta  61 gtagcggcga tcggtcacgt tgtcgatgcc gaacgacgcg agccagtgcc gatcgaaccg  121 atagcgcgcc ttcaggtcga cgaccatgaa cgagctggtg ccgccgtata cgcccggatt  181 cacgtcgctg ttgtcgagcg cgttgtactg ccgccccgaa tagcgaacgc cgacgctcgt  241 catccaatgc tcgtcgaagc ggtacgacgc gagcaggttc gcgcgcatcc gcggaatccg  301 cggccagcgc gcgccgacgt agttcggatt ggccgcatcg gcaagcgtct gcgcattcgt  361 cgcggacacg ttcgcgtcga cgtcgagccc cttgagcgcg acgtcctgcc ccgagaacgc  421 gagctcgacg ccgcgcaccc gcacgcggtc gacgttcgag acgttcgtgt acgtcgacgc  481 gccggcaagc gtggtctggc tgtagatcga attgcgcaga tcgctctgga acacgctcgc  541 gcgcacgacg ccgaagccga cgtcgcgctc ggcggtgaaa tcccagtcga tcgccttttc  601 cggctgcaga ttcgggttgt tgttgacgat cgcgttgttc gagatcgtgc cctggaacag  661 ctcggcgacc gtcggaaagc gcgtgcccgt cgcgaacgac aaccgcaggc gccacgcgct  721 ggccggctgc cattcgagcg agagcttcgg cgagaacgcg gtcgcgccgc gctccgcgta  781 gccgagcgtc gcggtcgcgc cgccgagctc gccgccgtat gcatcccagc gctcgtagcg  841 caggccgagc gtcgcgagcc agtcgggcgc gacgcgccac gcgtcctgcg catacagcgc  901 ctgcgtgcgc gtgttgccgc gatagcggtt cacgagcgtc gtcggcaccg cgttttgcca  961 gtcggccgtg ttgtaggtcg cgttgcgcag gaaatacgta tcgaagtggt agccgaacga  1021 gaaacggtgc ccgcgcacgt cgggcgactc cgcgcgcaag tcgacggtgc gccagccggt  1081 gccgtcgcca tggaacaccg tgcccggccc gccgtcccag gcgccgggcg gtgcgccgga  1141 cgcgctgcgc agcacgtcgc gcgacacctc gtaggcggac gcgatggccg acagcttcca  1201 gccggacgcg agccgcgcgt cgagcccgag cccgtacagc cagttctcct ggtcgcccgt  1261 ctgcggcgcg aacgcgcccg gcgacaccgt gtagttgcgc ccgccgaacg acacgttgcc  1321 gccgtacacc gggttgcccg ccgcgtcgcg caggaacgtg tcgccgtgct gccggtagtg  1381 attctcccag tggccgagcg tgaccgtcgc gtcgacgtga tcggtgaacg cgtagccgaa  1441 gcgcaacgtc tcgttgagct gctcggtgcg ctcgatcgtc tgcgcgccga cgatcgtccg  1501 cggccggccg ttcggcccga tgtcggagac ggcccccgtc accggcacgg gtgcgccgag  1561 cctcggatcg aacgcgccgt tcgggctcgc gtattgcatc ggctggctgt cgttctcgag  1621 ccggtcgagc gacagcgcat accagaaacg tccgacgcga tcggcgatgc gcgcgctctg  1681 gtgattgccg ccgaagctgt cggcgaagcc gtagccgtcg cgatagcgct gcgtgaagaa  1741 ctgcgtcgac accgacgcct cgagccgctg cggcttgtgc gtggtgatct gcacggtcga  1801 gccgatcgcg ttgcccgggt agagcgcgga aaacgggccg tacagcacgt ccacgcgtgc  1861 gatgtcgtcg ggctggatca gcgaccagcg cggcggatac gaatagctgg aaccgagcag  1921 attggacagc aggatgccgt ccgcatagac gagtccgcgc gcgctctgca gctcattgaa  1981 gtctcggccg gcgaacactg aattgcggtc gccgatgtag cgccggcgca ccatcaggtt  2041 cggcgcgtac ttgagcgcgt cttcggtcgt cacgttgacg tgcgaatcga tctgctcgcg  2101 cgtgagcgcc tcgacgacgc cgggcgtgtc gggcgcgaac gcggcgcgct gcgcggtgac  2161 cttgacggta tccaaggttt tcccggatgc ggtcatctcc gcggcggcgg ccggcgttga  2221 atcggcaggc acggcggcgg ccgtcgaagc cgcgtgggcg gcgggccagg cgagcgccgc  2281 ggcgcaggcg gccgcgagcc gcctgcgcgc gccgggcgcg gcaagttgat ggaacat  118 1 mfhqlaapga rrrlaaacaa alawpaahaa staaavpads tpaaaaemta sgktldtvkv  61 taqraafapd tpgvvealtr eqidshvnvt tedalkyapn lmvrrryigd rnsvfagrdf  121 nelqsarglv yadgillsnl lgssysyppr wsliqpddia rvdvlygpfs alypgnaigs  181 tvqitthkpq rleasystqf ftqryrdgyg fadsfggnhq sariadrvgr fwyalsldrl  241 endsqpmqya spngafdprl gapvpvtgav sdigpngrpr tivgaqtier teqlnetlrf  301 gyaftdhvda tvtlghwenh yrqhgdtflr daagnpvygg nvsfggrnyt vspgafapqt  361 gdqenwlygl gldarlasgw klsaiasaye vsrdvlrsas gappgawdgg pgtvfhgdgt  421 gwrtvdlrae spdvrghrfs fgyhfdtyfl rnatyntadw qnavpttlvn ryrgntrtqa  481 lyaqdawrva pdwlatlglr yerwdaygge lggatatlgy aergatafsp klslewqpas  541 awrlrlsfat gtrfptvael fqgtisnnai vnnnpnlqpe kaidwdftae rdvgfgvvra  601 svfqsdlrns iysqttlaga stytnvsnvd rvrvrgvela fsgqdvalkg ldvdanvsat  661 naqtladaan pnyvgarwpr iprmranlla syrfdehwmt svgvrysgrq ynaldnsdvn  721 pgvyggtssf mvvdlkaryr fdrhwlasfg idnvtdrryy vfhpypgrtf ygelkwsl  624 78 124382059 YP_001025431 139 1 atggcgcccg cggcgacgcg ggcgccgagc cgtcgtatcg atgcgctgcc tcacgggcct  61 tcctgtgcgc tgatattgat acgttatatc gtttcctgtt catcgcgcga ttcggcccgt  121 gattccgtcc accccgacca caccacaccg attaccgcaa tggatgacca ccgacgtatc  181 gcgccgcctt tcgctcgcag gctgcatccc ttgtcgctgc tgctcgccgc gagcctcgcg  241 catggcgaga cgggcgcgcc gcccgccgag cgccgatcgg acgcgccgcc cgcgacggcg  301 ctcgcgccga tcttcgtgac cgcgaacccg ctcggcgcgt cggcgctgag ctcgcccacc  361 gcgtcgctgt ccggcgacgc gctgacgctg cgccgcaccg attcgctcgg cgacacgctg  421 aacggcctgc ccggcgtgtc cacgacgacc tatggcccgc tcgtcggccg tccgatcatc  481 cgcgggatgg acggcgatcg catccgcctc ctgcagaacg gcgtcgccgc gtacgacgcg  541 tcgtcgctgt cctacgatca cgcggtgccg caggatccgc tgagcgtcga gcgcatcgag  601 atcgtgcgcg ggccggcggc gttgctgtac ggcggcaacg cggtcggcgg cgtcgtcaac  661 acgatcgaca accggattcc gcgcgaagcg atcacgggcg tctcgggcgc gctcgacgcg  721 agctacggcg gcgcgaacaa cgcgcgtgcg ggcgcggcgc tcgtcgaagg cggcaacggc  781 cgcttcgcgt ttcatctcga cgcgttcggc cgcgagaccg acgcgctgcg gattccgggc  841 catgcgcatt cggcgcgcca gcgcgcgctc gacggcgagg acgccagcga gccgtacggc  901 aggctgccga acagcgacgg ccgccgttac ggcggcgcgg cgggcggctc gtacacgtgg  961 gcggacggct acgtcggcgc atcgtacagc ggctacgaat cgaactacgg ctccgtcgcc  1021 gaaaccgacg cgcggcttca gatgcggcag gagcgcgtcg cgctcgcgtc ggaggtgcgc  1081 aatctgcgcg ggccgttctc gcagctgaaa ttcgacttcg gctacacgaa ttaccagcat  1141 aaggaaatcg aggacggcgt gacgggcacg acgtttcgca atcacggcta cgaggcgcgc  1201 gtcgaggcgc ggcaccgcaa gctcggcccg ttcgaaggcg cgctcggcgt gcaggtcgga  1261 cagaacacgt tctccgcgct cggcggcgag gcgctcgcgc ctaccacgcg cacgacgagc  1321 gtcgcgctgt tcggcctcga gcagtggcag gcgaccgacg cgctgaagct gtccgccggc  1381 gcgcgcatcg agcacgtgcg gctcgatccg agcgcgaacg gcgacgacaa gttcggcttc  1441 gcgcgctcgc gcgatttcaa tgcgggcagc gtgtcggcgg gcgcgctgta ccagctcgcg  1501 cccgcgtggt cgctcgcggg caacgtgtcg tacacggagc gcgcgccgac cttctacgag  1561 ctgtacgcga acggcccgca cggcgcgacg ggacagtacc tgatcggccg gcccgatgcg  1621 cagaaggaga aggcggtgtc gaccgatctc gcgctgcgct atgcgagcgg tccgaaccgt  1681 ggcagcatcg gcgtgttcta cagccgcctg cggaactacc tcgccgaata cgacaccgga  1741 cggctcgtcg acgacgacgg cgtgcccgtg gccccgggcg ccgacgacgc gctgcgcgag  1801 gccgtctatc gcggcgtgcg cgcggagttc tacggcgtcg agctcgaagg caggtggcgc  1861 gcgttcgaac ggcgcgggca tcgcgtcgat ctcgagctga gcgccgacta cacgcacgcg  1921 cgcaacgcgg acacgggcga gccgctgccg cggatcgcgc cgctgcgcgc gacgctcgcg  1981 gccgattacg gctacggccc gttcggtgcg cgcgcgcagc tcacgcatgc atgggcgcag  2041 catcgcgtgc ccgagcacga tctcgcgacc gacggctaca cgtcgctcgg cgtcgtgctc  2101 acctacaagt tgcgcgtcgg cgcgacgaac tggctcgcct atctgcgcgg cgacaacctg  2161 acgaaccagg acatccgcta cgcgagctcg gtggtgcgca acatcgcgcc gcagggcggg  2221 cgcagcgtga gcatcgggat gcgcacgaca ttctga  119 1 mapaatraps rridalphgp scaliliryi vscssrdsar dsvhpdhttp itamddhrri  61 appfarrlhp lslllaasla hgetgappae rrsdappata lapifvtanp lgasalsspt  121 aslsgdaltl rrtdslgdtl nglpgvsttt ygplvgrpii rgmdgdrirl lqngvaayda  181 sslsydhavp qdplsverie ivrgpaally ggnavggvvn tidnriprea itgvsgalda  241 syggannara gaalveggng rfafhldafg retdalripg hahsarqral dgedasepyg  301 rlpnsdgrry ggaaggsytw adgyvgasys gyesnygsva etdarlqmrq ervalasevr  361 nlrgpfsqlk fdfgytnyqh keiedgvtgt tfrnhgyear vearhrklgp fegalgvqvg  421 qntfsalgge alapttrtts valfgleqwq atdalklsag ariehvrldp sangddkfgf  481 arsrdfnags vsagalyqla pawslagnvs yteraptfye lyangphgat gqyligrpda  541 qkekaystdl alryasgpnr gsigvfysrl rnylaeydtg rlvdddgvpv apgaddalre  601 avyrgvraef ygvelegrwr aferrghrvd lelsadytha rnadtgeplp riaplratla  661 adygygpfga raqlthawaq hrvpehdlat dgytslgvvl tyklrvgatn wlaylrgdnl  721 tnqdiryass vvrniapqgg rsysigmrtt f  625 43 124383587 YP_001028404 140 1 atgcgcgtcg aacgggttcc ataccgctta atcactgtcg cgacggctgc cgttttcctg  61 gccgcgtgcg gaaaaaaaga atcggctccg ccccctcaaa cgcccgaagt cggcgtcgtc  121 accgtccagc cgcagcccgt gccggtcgtc tccgaactgc cgggccgtac gagcgcctat  181 ctggtcgcgc aggtgcgcgc acgggtcgac ggcatcgtgt tgcggcgcga gttcacggaa  241 ggcagcgacg tcaaggccgg ccagcgcctg tacaagatcg atccggcacc ctatatcgcg  301 caattgaaca gcgcgaaggc gacgctcgcg aaggcgcagg cgaacctcgc gacgcagaac  361 gcgctcgtcg cgcgctacaa ggtgctcgtc gccgcgaacg cggtcagcaa gcagcagtac  421 gacgatgcgg tggccgcgca agggcaggcg gccgccgacg tgggcgcggg caaggccgcc  481 gtcgagaccg cgcagatcaa cctcggctat acggatgtcg tctcgccgat cacgggccgc  541 gtcggcatct cgcaggtcac gccgggcgcg tacgtgcagg cgagccaggc gacgctgatg  601 tcgaccgtcc agcagctcga tccggtctac gtcgatctca cgcagtcgag cctcgacggc  661 ctgaagctgc gccaggacat ccagagcggg cgcatcaaga cggaaggccc gggcgcggcg  721 aaggtcacgc tgattctcga ggacggcaag ccgtacccgg agcggggcaa gctgcagttc  781 agcgatgtca cggtcgacca gacgacgggc tcggtcacga tccgcgcgat cttcccgaac  841 aagcagcgcg tgctgctgcc gggcatgttc gtgcgcgcgc gcatcgaaga gggcgtcaac  901 gagaacgcgt tcctcgttcc gcagatcggc gtcacgcacg atccgaaggg ccaggcgatc  961 gcgatgatcg tcgacggcaa gggcaaggtc gagccgcgcg tgctggtcac gggcggcacg  1021 cagggccaga actgggtggt cgagagcggc ctgcaggcgg gcgaccgcgt gatcgtgcag  1081 ggcatcgaca aggtgcgccc gggcatgacc gtgaaggccg ccgaggctca attgccggcc  1141 gcggcggccg gcgcgtcggg tgccgcgccc gcgggcggct cgcccgccca ggccgcggcg  1201 gcgagcgcgg ccgcgtcggg tgccgcgccg tcgagcgctg ccgccgcgtc gagcgcgcaa  1261 taa  120 1 mrvervpyrl itvataavfl aacgkkesap ppqtpevgvv tvqpqpvpvv selpgrtsay  61 lvaqvrarvd givlrrefte gsdvkagqrl ykidpapyia qlnsakatla kaqanlatqn  121 alvarykvlv aanayskqqy ddavaaqgqa aadvgagkaa vetaqinlgy tdvvspitgr  181 vgisqvtpga yvqasqatlm stvqqldpvy vdltqssldg lklrqdiqsg riktegpgaa  241 kvtliledgk pypergklqf sdvtvdqttg svtiraifpn kqrvllpgmf vrarieegvn  301 enaflvpqig vthdpkgqai amivdgkgkv eprvlvtggt qgqnwvvesg lqagdrvivq  361 gidkvrpgmt vkaaeaqlpa aaagasgaap aggspaqaaa asaaasgaap ssaaaassaq  BcAU1054-700 ID MW Protein GI# Protein Locus# SEQ ID NO: Sequence 701 81 107022717 YP_621044 165 1 atgaaaaaag tggagcagaa gaagatggag tgggcaacag gcacgcgttt gcgagagatc  61 gcagccgcgg caagcgtggc gttcggtacg gcggcggcag ggcacgcata cgcccagacg  121 gcgccggccg tgaacgcggg cgccacggcg tcggccagca gtgcgcaaac cggcgcgacg  181 gcgacgacgt cgaccagcgc gcaaaacggc acgctgccgg cgatcaccgt caacgcggcc  241 tcggcaggtg acggcacggt cgggctcgtc gcgaagcgca gcacgaccgg caccaagacc  301 gacacgccgc tcaacgagat cccgcagacg atcaacgtcg tcaccgcgca gcagatcgag  361 atgaccggcg ccaccgacgt gaaagaggcg ctgcgctacg tgccgggctt ctcgtcgtac  421 gggtcggaca accgctccga ctggtacgcg gcgctgcgcg gcttcacgcc gaccgcctac  481 gtgaacgggc tgcaggtgcc gaacacgatc aacctcgcga gctggcgcgt cgatccgtac  541 atgatcgaca gcatcagcgt gctgcgcggg ccgacctcgg tgctgtacgg agagggcgat  601 ccgggcgcga tcatcgacgt gcacaccaag ctcgccgacg gcgagcgcgt gcgcgaagca  661 ggcgtgcaga tcggcaacta cgcgcgcaag cagttcatga tcgacgtcgg cgacaagctc  721 gacccggacg gcaagtatgc gtaccggttc gtcggcgtcg cgcgcgacgg caacgcgctg  781 acgggcccga acaacgacca gcgcgtcgcg ctcgcgccgt cgttccgctg gcgcccggac  841 gcggatacgt cgctgacgct gtccgcgacc tacctgcagg actggggcga catctcgtcg  901 aacttcctgc ccgcgcaagg cacggtgctg ccgaacccga acggccagat caacaaggac  961 atctacgaag gcgacggcaa cttcaactac taccgcaaga agcagtggtc ggtcggttac  1021 cagttcgagc gcaacctgac gccggcctgg acgttccgcc agaacacgcg gctgatgcac  1081 ctgtcgctcg acaacggctc ggtgttcggc aacggcttcg tcgaaggcag cacgaccgac  1141 gtgtcgcgct gggccggcgt gttccagatg aactacagcc gcttcgacat cgacaacaac  1201 ctggagggcc gcttcgcgac gggcccgttg cagcacacgc tgctgctcgg cttccagtac  1261 aaccgccaga ccgcgaccga cagcgaatgg ctcgccgccg cgccgccgct gaacatctac  1321 aacccggtgt accagcccgt cacgacggcg gtgttcacgc ccgacgcgac gatccgcacc  1381 aacacgtaca cgacgatcaa cacgttcggc ctgtacgcgc aggaccagat caagtggaac  1441 cgctggacgc tgacgctcgg cggccgcgag gactgggtca acatgcggat ggacgaccgc  1501 gcggccggca cgtcgacgaa ggcggacgtc acggcgttca ccggccgcgt cggcctcacg  1561 taccagggcg actacgggct gtcgccgtac gtcagctacg cgacgtcgtt caatccgctg  1621 atcggcgtga acctggtcgg cggcgggctg ccgcagccga cgcgcggcaa gcagatcgaa  1681 gccggcctgc gctggcagcc gcccggcaag aacctgatgc tgaacgcggc gatctaccag  1741 atcaaccaga ccaacgtgct cacgtcggcg ctgccgagcc aggacccgac cggcacgaag  1801 tcggtgcaga cgggcgaagt gcgctcgcgc gggatcgagc tgagcgcgac cggcaaggtc  1861 acgcgaaacc tgtcggtgat cgcgtcgtac gtctatcagg acgtgaagaa cgtgcaggcc  1921 aacgacgtct cgctgaacaa ctggccggtc gacattccgc gtccgcgcca gatggcgtcg  1981 ctgtggaccg actggacctg gtacacgggg ccgctcgccg gcttcggcct cggcggcggc  2041 attcgctacc agagcgcgtc ggccggtgcg gccgacaact cgctgacggt atcgagcgtc  2101 acgctgttcg acgcgggcct gcattacgac gtgcgtaact ggcgcttcgc cgtgaacggg  2161 acgaacctgt tcaaccgcca ttacatcagc ggttgccagt cgaacaacgt gtgcattttc  2221 gggaccgacc gcaccgtgat cgcgaccgcg aaatacaact ggtga  141 1 mkkveqkkme watgtrlrai aaaasvafgt aaaghayaqt apavnagata sassaqtgat  61 attstsaqng tlpaitvnaa sagdgtvglv akrsttgtkt dtplneipqt invvtaqqie  121 mtgatdvnaa lryvpgfssy gsdnrsdwya alrgftptay vnglqvpnti nlaswrvdpy  181 midsisvlrg ptsvlygagd pgaiidvhtk ladgervrea gvqignyark qfmidvgdkl  241 dpdgkyayrf vgvardgnal tgpnndqrva lapsfrwrpd adtsltlsat ylqdwgdiss  301 nflpaqgtvl pnpngqinkd iyegdgnfny yrkkqwsvgy qfernltpaw tfrqntrlmh  361 lsldngsvfg ngfvegsttd vsrwagvfqm nysrfdidnn legrfatgpl qhtlllgfqy  421 nrqtatdsew laaapplniy npvyqpvtta vftpdatirt ntyttintfg lyaqdqikwn  481 rwtltlggre dwvnmrmddr aagtstkadv taftgrvglt yqgdyglspy vsyatsfnpl  541 igvnlvgggl pqptrgkqie aglrwqppgk nlmlnaaiyq inqtnvltsa lpsqdptgtk  601 svqtgevrsr gielsatgkv trnlsviasy vyqdvknvqa ndvslnnwpv diprprqmas  661 lwtdwtwytg plagfglggg iryqsasaga adnsltvssv tlfdaglhyd vrnwrfavng  721 tnlfnrhyis gcqsnnvcif gtdrtviata kynw  702 55 107025374 YP_622885 166 1 atgaataact tgcacaacac gaacggcctg atgcgcttcg cgaaggtggc ggccgcgagc  61 accctgctcg cgacgctgct cgccgcgtgc gcggtcggcc ccgattacaa gcgtccggac  121 gcggcggcgc ccgccgcgtt caaggaagcg ccgaccctcg ccgccggcga gcaggccggc  181 acgtggaaga cggccgagcc ggcggacggc gaacatcgcg gcgaatggtg gaaggtgttc  241 ggcgacccgg tgctcgattc gctcgagacg caggcgctcg ccgcgaacca gaacctgaag  301 gccgcggccg cgcgggtcga ggaagcgcgt gcggcgaccc ggtcggcacg ctcgcaatgg  361 ttcccgcagg tcggcgctgg tttcgggccg acgcgcgagg ggctgtcgtc ggcgtcgcag  421 ttccagccgc agggcaccgg cccgaccaac gcgacgctgt ggcgcgcgca gggcacggtg  481 tcgtacgaag ccgacctgtt cggccgcgtc ggccgcaacg tcgaggcgtc gcgcgccgac  541 caggcgcaga gcgaagcgct gttccgctcg gtgcagctcg cgttgcaggc cgacgtcgcg  601 cagaactact tcgaattgcg tcagctcgat tcggatcagg acctgtaccg ccgcacggtg  661 gagttgcgcg agcaggcgct gaagctcgtg cagcgccgct tcaacgaagg cgacatcagc  721 gagctcgacg tgtcgcgcgc gaagaacgaa ctggcgagcg cgcaggccga tgcggtcggc  781 gtcgcgcgcc ggcgcgcggc gtccgagcat gcgctggcca ttctgctcgg caaggcgcct  841 gcggatttcg cgttcaagga aacgccgatc gtgccggtcg cggtgaagat tccgccgggc  901 ctgccgtccg cgctgctcga acgccgcccg gacgtgtcgg cggccgagcg cgcgatggcg  961 gccgcgaacg cgcggatcgg gctcgcgaag tcggcgtact tcccgaagct cgatatcacc  1021 gggtcgttcg ggtatgaagc gtcgacgctc ggcaacctgt tcctgtggtc gagccgcacg  1081 ttcctgctcg ggccgttcgc cggcaccgcg ctgacgctgc cgctgttcga cggcgggcgg  1141 cgggctgctg gtgtgcagca ggcgcgcgcg cagtacgacg agcaggtcgc gaactaccgg  1201 cagcaggtgc tcgtcgcgtt ccgcgaggtc gaggacaatc ttgctgatct gcgtttgctc  1261 gatgatcaga tccgcgcgca ggatgcggcc gtcaacgcgt cgcgtcgggc ggcgacgttg  1321 tcgcgcacgc aatatcagga aggtgaggtc gcgtatctcg acgtgatcga cagtgagcgg  1381 tcggtgttgc agtcgcagtt gcaggcgaac cagttgacgg gggcgcaggc ggtgtcgacc  1441 gtcaacctga tccgcgcgct gggcggcggg tgggggaatg cgccggcgcc gacggcggtg  1501 ggtgatgcgg cgtctggcaa ggctgacgta gctgcgcggt aa  142 1 mnnlhntngl mrfakvaaas tllatllaac avgpdykrpd aaapaafkea ptlaageqag  61 twktaepadg ehrgewwkvf gdpvldslet qalaanqnlk aaaarveear aatrsarsqw  121 fpqvgagfgp treglssasq fqpqgtgptn atlwraqgtv syeadlfgry grnveasrad  181 qaqsealfrs vqlalqadva qnyfelrqld sdqdlyrrtv elreqalklv qrrfnegdis  241 eldvsrakne lasaqadavg varrraaseh alaillgkap adfafketpi vpvavkippg  301 lpsallerrp dvsaaerama aanariglak sayfpkldit gsfgyeastl gnlflwssrt  361 fllgpfagta ltlplfdggr raagvqqara qydeqvanyr qqvlvafrev ednladlrll  421 ddqiraqdaa vnasrraatl srtqyqegev ayldvidser svlqsqlqan qltgaqavst  481 vnliralggg wgnapaptav gdaasgkadv aar  703 84 107025915 YP_623426 167 1 tcagtagcgc gcgttcagcg tgacgaacgc cgaacggccg ggcgcgatcg acgcatagtg  61 cgccggatac gcctgatcga agtaggtgcg gttgaacagg ttgttgacgt tcagttgcag  121 gtcgagcttc ttgttgatcc ggtactgcgc catcgcgtcg aagcgccagt acgacggcac  181 ggcgcgcagg ttcgcgggat cgccgaatac ttccgacata tagaacgcgc cgccgccgac  241 cgtgaacttc ggcgtgacgt cgtagttcga ccacatcgtc aggctgtgct tcggcgtgtt  301 cgggaagcgg ttgccgttgt tcgcggcgtc cttgccgttg tcgcgcagtt ggctcttcat  361 gtacgtgtag ccgccgaaca cctgccattg cttcgtgatc tggccggcga ggccgagctc  421 gagaccctgc acgcgcttgt tgctgaccat cgcgtactgg ttgttcggca gcgtcacgcg  481 tgcgttggtc gtgtcgatct ggaacagcgc ggccgtcagc gacagcttgt cgttcagcac  541 gttccacttc gtgccgagct cgatgctgcg gtttttttcc ggcgaaagct gatcggcgtt  601 cgggccgacg ccgccgcggc ccggcgtgag cgactgcgtt tcgctgcctt cgccgagcat  661 catgccggcg ggcgtcgacg aggtcgcata cgacgcgtag atgctgccgt tctgcgcggg  721 cttgaacacg aggccggcct gccagttgaa gagcgtgtcg tcgcgcgtat aggtcttgcc  781 gccattcgcc ttggtatcgg tgaagcgggt cgagtagtcg tcgacgcgca cgccggcgtt  841 gacttgccag cgcggcgtga tctcgatcgt gtcgaagccg tagatcgact tcgtcgtggt  901 gcgcgcatgc gcgtagtcgt tgttgcgcgt gatcgaaccg gcccacggat cgttcgggtt  961 cggcgaccac aggctcgtgc agttgtagcc cgacgcggcg ccgatgcctt tctggcagat  1021 cgtgcccttg tcggtcgcga ccgtgtacga atcgcgcttg ccccattcgc gcgacagctc  1081 gataccggtc gtgaagctgt gcttgaacgg gcccgtgcgg aattcgccga acagctcggt  1141 caggttcgcg aggctgttga tcgagctgtt gcggttgttg ttgcggcgcc agaccttgcc  1201 gttcaccacg ttgccctggc tgtcgtccgg ctgcgtccag atgtagtcct gtgtcgattc  1261 cgtgtagcgc gtggtgttgc gcaccgtgag gttcggcgtg atgtcgtgct cgatcttgat  1321 cgtgctgatg tccgacgtgg tcttgcggaa gtcgcggtcg atcaggccgt agaagttgtg  1381 gcgatcgacg ggcgccggat agatcgtgcc gacgttcgcg ggcttgttcg acgtcgtgta  1441 gaagtacggg atgccgccgt cgggcatgtc gtccgtctgc aggtggtagt agctcgcggt  1501 cacgcgcgtc ggcgtgccga ggccgaacgc gatcgacggc gcgacgcccc agcgctcgtt  1561 gttgacggca tcgcgaccgg cgacgtcgtt gttgtggctc atcaggttca ggcggaacgc  1621 ggcgtgctcg gcgaactgcc agttgccgtc ggcggtgaag cggcgatagc ggtcggtgcc  1681 gaggcccgcg ctcgcggcgg ccgtcgtgcc gaggtgcggg gccttcgtga tcaggttgat  1741 gctgccgccg gcgccgccgc ggccgccgta cgcgccgtcg gaacccttcg tgatctcgac  1801 gcgttcggta ttgaagatct cgcgcgtggt ggcgcccgtg tcgcgcatgc cgtcgacgaa  1861 catgctgccc tgcgtgtcgt agccgcgaat gaacggacgg tcgccgagcg ggttgccgcc  1921 ttcgccggcg ccgaacgtga tgccgggcac ggtgcgcagc gcttcggtca gcgtcgccgc  1981 gccgctgctc tggatcagtt cctgcggaat cacggtgacg gatttcggcg tgtcgacgag  2041 cggcgcggtg aatttcgcgg aagcggaaaa gtcggccttg tagctgtgct cggtcttgcc  2101 ctggatctcg atcggcgcga ggtggccttc ggtgctggcg ggcgccgcgg gcggcgtacc  2161 gtcggcgaac gcggggctcg cggcgagcac gctgcacaag gtggtgaact taccgagctt  2221 cagctcgtcc ggacgggatt tcat  143 1 mksrpdelkl gkfttlcsvl aaspafadgt ppaapasteg hlapieiqgk tehsykadfs  61 asakftaplv dtpksvtvip qeliqssgaa tltealrtvp gitfgagegg nplgdrpfir  121 gydtqgsmfv dgmrdtgatt reifnterve itkgsdgayg grggaggsin litkaphlgt  181 taaasaglgt dryrrftadg nwqfaehaaf rlnlmshnnd vagrdavnne rwgvapsiaf  241 glgtptrvta syyhlqtddm pdggipyfyt tsnkpanvgt iypapvdrhn fyglidrdfr  301 kttsdistik iehditpnlt vrnttrytes tqdyiwtqpd dsqgnvvngk vwrrnnnrns  361 sinslanite lfgefrtgpf khsfttgiel srewgkrdsy tvatdkgtic qkgigaasgy  421 nctslwspnp ndpwagsitr nndyahartt tksiygfdti eitprwqvna gvrvddystr  481 ftdtkanggk tytrddtlfn wqaglvfkpa qngsiyasya tsstpagmml gegsetqslt  541 pgrggvgpna dqlspeknrs ielgtkwnvl ndklsltaal fqidttnarv tlpnnqyamv  601 snkrvgglel glaggitkqw qvfggytymk sqlrdngkda anngnrfpnt pkhsltmwsn  661 ydvtpkftvg ggafymsevf gdpanlravp sywrfdamaq yrinkkldlq lnvnnlfnrt  721 yfdqaypahy asiapgrsaf vtlnary  704 83 107027766 YP_625277 168 1 tcagaaatcc actttcatgc tgacggcgac cgtgcggccg ggcgacgaat atgcgtcgag  61 cacctgcgaa tcggccgcga tgccgcgcac gtccgaccag ttccagtact tgcggtcgaa  121 caggttgcga atgccgatcg tcgcgctcac gtgcttgttg aagcgatagc cgccgcgcag  181 gtcgacgacg aacgacgacg gcggcgtgaa gcacgccttg ttcgaacagt cggacttgtc  241 gacgtccttg tcgcgtttgg cggcctggaa cagcaggtcg gtctgcacga accagcgttc  301 ggtcggctcg taccgcacgc cgaacacggc cgagaacggg ttcacggtat tgagcggctg  361 gctcgccgcg ccgtcgttct gcgtcgagcc cttcgtgaac gccatcgccg tcttcagcgt  421 gatgccgttc ggcatcaccc attcggcgcg gccttcgagg ccgtggatgc gcgcgtcggc  481 gaagttcacg tactggaaca cgaacgggtc gaccggccgg ccgctgccgg cgagcgtcgt  541 acgcgagatg aagttgcggt agcggccggt gaaggcggcc gcgctgtagc gcacgacgcc  601 gtagccggtg ccggccttgc cgcgcaggcc ggcttcgaac gtgtcgctgg tttcgggctt  661 caggttcgga ttgccgatcg acgtgtagcc gtacaccggg ttcgagaagc tgctgttcac  721 ctggtcgggc gtcggcgcgc ggaagccgtg cgcgtactgc acgtacggaa tgaccgctgg  781 agtgatttcg tagagcacgg cgacgcgcgg cgacagctcg ttggcgctcg tcgacacggc  841 cttgccggtg aacagcggat cgttttcggt cgggctcagc cggtacgtgt cgaagcgcag  901 gcccggcgtg acgagcaggc ggccgtagcc gatctggtcc tgcacgaacg cgccgaacag  961 cgtgtagtcg gtgtcgggaa acgccttgtt cgggaacgct tcgccgacgc ccggcaccgt  1021 gccgtcgcgc aggttcgtca cgcgcgacag gctgccgtcg acgccgtaca acagcttgtg  1081 cgcgagcggg ccggtcgaaa agccgctttc ggcgaacgcg gcgccgccga acgtgcgttc  1141 cttgtactgg ttgtcgcgcg aacgcgaggg tagcctgccg cgcgtctcga acgcgtactg  1201 gtcctgcttc gcgtcctggt agtagaactg cacgtgcgcg gtctggaacc agcggaacgc  1261 gtcgtcgcga aagtcgtagt cgacgctgaa gcggttgcgc tcgagccggt cgtgggtcgt  1321 gaggccgagc gtggccggcg cattgatcgc cgacagcacg tcggtgctca cgcgccgctg  1381 caccgtttcg gcggtgaact tgatcgtgtc gcgtgtggtg ggcgtcagca cgagcttgcc  1441 gagcagcgat tccgaataca cgtcctgcgg attcgacgtc gtgcgcagcg tgctcgccga  1501 attgttgtcg ccgcgcgtgt cgacctcgtg gccgcgccgg ccgtcggcga tgatcatccc  1561 ctggatgcga tcgttgccgc ccgcggccga tacggtcgcg ccgatgctgc ggtcggtcga  1621 gtcgtagctc ggccggaacg agaagtagta aggcttgtgg tagatcgaca gcaggtcgcg  1681 cgggtccttc gtgatgaagt tcaccgcgcc ggtcaggccg tcgctgccgt acagcgccga  1741 cgccggcccg cgcaggatct cgatgcgctt gagcgtgtcg agatcggcgt agtcgccgcg  1801 gcccgcttcg agcggaccga acgagaacgc gttcggcagg cggatgccgt cttccatcag  1861 cagcacgcga ttgccttcga ggccgcggat gttgatgctc gaatcgccgt cgcggccgcc  1921 gccgagcgcg gcgctgccgg gccggtacgc ggtgcggcgc accgtgatgc ccggctcgta  1981 gcgcagcgcg tccttgatat tggtggcctg ttgctcttcg agatcctcgt cggtgatcac  2041 cgacacggac gccgccgtgc ggctcgccgc ggtggcggtg cgggtggccg tgacggtgac  2101 gggatcgagc agcgccgcct cgccgcgcgc ggatgcggcg gtcaggacgg cggaaggcgg  2161 gggggcgcct tgcggcgacg aatcggcgtg agccggggcg gcggacaggc cgaacgcgcc  2221 gaacagcgcg gcacagatcg gccgccgcgc cagcgtatag caatgcac  144 1 mhcytlarrp icaalfgafg lsaapahads spqgapppsa vltaasarge aalldpvtvt  61 atrtataasr taasysvitd edleeqqatn ikdalryepg itvrrtayrp gsaalgggrd  121 gdssinirgl egnrvllmed girlpnafsf gpleagrgdy adldtlkrie ilrgpasaly  181 gsdgltgavn fitkdprdll siyhkpyyfs frpsydstdr sigatvsaag gndriqgmii  241 adgrrghevd trgdnnsast lrttsnpqdv ysesllgklv ltpttrdtik ftaetvqrrv  301 stdvlsaina patlgltthd rlernrfsvd ydfrddafrw fqtahvqfyy qdakqdqyaf  361 etrgrlpsrs rdnqykertf ggaafaesgf stgplahkll ygvdgslsrv tnlrdgtvpg  421 vgeafpnkaf pdtdytlfga fvqdqigygr llvtpglrfd tyrlsptend plftgkavst  481 sanelsprva vlyeitpavi pyvqyahgfr aptpdqvnss fsnpvygyts ignpnlkpet  541 sdtfeaglrg kagtgygvvr ysaaaftgry rnfisrttla gsgrpvdpfv fqyvnfadar  601 ihglegraew vmpngitlkt amaftkgstq ndgaasqpln tvnpfsavfg vryepterwf  661 vqtdllfqaa krdkdvdksd csnkacftpp ssfvvdlrgg yrfnkhvsat igirnlfdrk  721 ywnwsdvrgi aadsqvlday sspgrtvavs mkvdf  705 81 107025731 YP_623242 169 1 atgcggtacg aggtacggga acctgagaga tcgatgatgc agggcgcacg cgtcgcccgt  61 ttcgggcgct ggcgtccgtc gctgtacctc ggcctgctga tcgccgcgca tccggcggcg  121 gcgttcgccg atgcggcgcc ggcggccggc gcggaagtcc gccgggacaa agcggccgcc  181 gcgcagcctg ccgggagcga gttgaaggcg attgcggtga atgcgtcgcg cggcgtcgcc  241 gacgatccgt ccgtggcgac ggtcggcaag atgccgctcg cgttgcgcga gattccgcag  301 tcggtcagcg tgacgacgcg cgagcggatc gaccagcaga acctgttctc gctcgacgag  361 gtgatgcagc agtcggccgg cgtgaccgtc cagccgtacg tgctgctcac cactgcgtac  421 ttcgtgcgcg gcttcaaggt cgattcgttc gaattcgacg gcgtgccggt cgtgatcggc  481 gacatggcga gcgcgccgca ggacatctcg gtgtacgaac gcgtcgaaat cctgcgcggc  541 gcgaacgggc tgctgcacgg ctcgggcaat ccggccgcga ccgtcaacct cgtgcgcaag  601 cgcccgcaat accagttctc cgcgcatgcg accgccagcg tcggcagctg ggaccgctat  661 cgcgcggaag ccgacatcgg cggcccgctc aatgcggccg gcaccgtgcg cagccggctc  721 gtcgccgcgt acgaggatcg ccacttcttc tacgaccacg cgaagcagga cacgcgttcg  781 atctacagcg tcacggaagt cgacgtgacg cgcgacacgc tgctcacgtt cggcgcgcag  841 taccagacca cgacatcggt gcccgacatg tccggcgtgc cgatggcgcg cgacgggtcc  901 agcctcggcc tgtcgcgctc gacgttcctc gacaccgcgt ggggccgttt caactgggac  961 acgacgcgcg cgttcgcgtc gatcgagcag aagctcggcg ccggctggaa ggcgaaggtc  1021 agcggcgaat accagagcgt gcgctcggac ctgaagtacg cgggttcgtt tggcgcgatc  1081 gatccggcga cgggcgccgg cggccggctg accggcgccg cgtaccagtt cagcagctac  1141 agccgcagca tcgatgcgaa cgtgcagggc cccgtgcatg cgttcgggct cacgcacgac  1201 ctgctgttcg gcgtcaccta cgcgaacagc agcagcgggc agatgaccgc gccgctgctc  1261 ggcgacgtgg ccggcacgcc ggtgaacgtg taccgctgga acccgagcag cgtgcccgag  1321 cccggcatcg ggccgtacca gcagtcgcag cagaacgacg tttcgcagaa gggcgtctac  1381 gggctgggcc gcatcaagct cgcggagccg ctgacgctcg tgctcggcgg ccggatgagc  1441 tggtggaacc aggacagcct cggcgcgcac tacaacaccg gccaccagtt cacgccgtac  1501 ggcgggctga tctgggattt cgcgcgcgac tggtcgtggt acgcgagcta tgcggaggtg  1561 ttccagccgc agaccaagtc gatgtggggc ggcggcatcc tgacgccggt gaaggggcgc  1621 acctacgaaa cgggcgtgaa gggcgaactg gcgggcggca agctcgacgt gtcgctcgcg  1681 gcgttccgca tcgatctcga caacaacccg caggtcgacc tcgcgcatcc gtgcgcgggg  1741 ccgagctgct actacgtgaa cggcggcagc gtgcgcagcc agggcttcga gttcgaggcg  1801 aacgggcgca tcacgccgtg gtggagcgtg tgggcgagct acacgtacga cacgatgcgt  1861 tatgcggaca acctcgcgaa cgcgggttcg ttcgcgccgc tgctgaatcc gcgccacctg  1921 ttccggctgt ggaccaacta cgacctgccg tggcaggagc ggcgctggag catcggcggc  1981 ggcgtgcagg tgcagagcag ctactcggcg caggcgaacg gcgtcacgat gagccagggc  2041 ggctacgcgc tcgcgagcgt gcggctcggc taccgctacg acaagcactg gagcgccgcg  2101 ctcaacgtca acaacctgtt cgaccgcacc tactacctga gcctgagcca gccgggctgg  2161 aacaaccgtt acggagaacc gcgcaacgtg atgctgaccg tgcgcgggca attctga  145 1 mryevreper smmqgarvar fgrwrpslyl glliaahpaa afadaapaag aevrrdkaaa  61 aqpagselka iavnasrgva ddpsvatvgk mplalreipq sysvttreri dqqnlfslde  121 vmqqsagvtv qpyvllttay fvrgfkvdsf efdgvpvvig dmasapqdis vyerveilrg  181 angllhgsgn paatvnlvrk rpqyqfsaha tasvgswdry raeadiggpl naagtvrsrl  241 vaayedrhff ydhakqdtrs iysvtevdvt rdtlltfgaq yqtttsvpdm sgvpmardgs  301 slglsrstfl dtawgrfnwd ttrafasieq klgagwkakv sgeyqsvrsd lkyagsfgai  361 dpatgaggrl tgaayqfssy srsidanvqg pvhafglthd llfgvtyans ssgqmtapll  421 gdvagtpvnv yrwnpssvpe pgigpyqqsq qndvsqkgvy glgriklaep ltlvlggrms  481 wwnqdslgah yntghqftpy ggliwdfard wswyasyaev fqpqtksmwg ggiltpvkgr  541 tyetgvkgel aggkldvsla afridldnnp qvdlahpcag pscyyvnggs vrsqgfefea  601 ngritpwwsv wasytydtmr yadnlanags fapllnprhl frlwtnydlp wqerrwsigg  661 gvqvqssysa qangvtmsqg gyalasvrlg yrydkhwsaa lnvnnlfdrt yylslsqpgw  721 nnrygeprnv mltvrgqf  706 88 107022421 YP_620748 170 1 atggccacca ccggcgcgct ggccgccgaa gctgcggcgt cggcgccgga tgaccggcac  61 gcgctgccgg cgatcaacgt caccgcgtcg tccgcgccgg ccgatccgct gacgcaaccg  121 ctcgaaacgg gctcgcggct cgggcttgcc agtctcgaca cgcccgcgag cgtcgaaacc  181 gtcacggccg acacgatcga cgcacgcggc gatcgcacgg tgctcgacgc ggtcacgcgc  241 acggccggct ttgcgagcgc gatcgcaccg ggcaccggcg gcaccgcact gagcgtgcgc  301 ggcttcagcg ggcaggaatc ggtgatgacg ctgcttgacg gcgtgcggct gatgccggcg  361 gccggcacga tcacgtttcc gttcgacacg tggtcggtcg cgcgcatcga ggtgctgcgc  421 ggcccggcat cggtgctgta cggcgaaggg gcgatcggcg gagtcgtgaa cgtggtgccg  481 aagcggccgc agcgcacgcg cgagacgacg ctgcaggcgg gcgtcggccc cgacggcgcg  541 aagcgcttcg cgttcgatac gacgggcgcg ctcggcccgc ggctgtcgta ccgcttctac  601 gcaagcgatg cgcgtgcgaa cggtctcgcc gagcgggccg acacccatac gacggccatc  661 ggcggcgcgc tgacattcga cgtgagcccg cagctcacgc tgacgctcga ctacgactac  721 gggcgccaga tgccggcgac ctactatggc gtgccggcgc cgcacggcgt gctcgatccg  781 tcgctgcgca agctgaacta cacggtcggc gacgcgacga tttcgtacta cgaccagtgg  841 acgcgcctgt cggccagcta ccggcccgcg cccggcgtga cgatcagcaa ccagctctac  901 tacctgacat cgaaccgtca ctggcgtaac gccgagtcat atgtgctcga cctcgcgacg  961 ggacgcgtca cgcgcggcga ctatctcgac atcggccatc atcagcggca gatcggcgat  1021 cggctgagcg cgcgtttcga cgggatgctg ttcggtcgcg cgaaccgttt cgtcgtcggc  1081 acggaattca gccagatcac gttcagcggt accaacaatt cgccgtatgg tggcgaaacg  1141 acggtgcccg cgcacggctt cgatcctggt gtgttcacga gcccggaccc gaccgtgccg  1201 caattcagca cgcgcgcacg gcaggcggcg gtattcgcgg agaaccggct cgaggtgctg  1261 ccgcggctcg cgtgggtgag cgggctgcgc tacgaccaca tcgcgttcag tcgcgagcag  1321 gcggcgaccg gcgcgggctt cgacaagcgg ttcgcgaacg tcggctggcg caccggtgtc  1381 gtgttcgata tcgcgccggc gttcaccgcc tatgcgcagt acacgacggg cgcggagggc  1441 gtcggctcgc tcgtgacgct gtccgcgtcg cagatgaacg tccggctcgc gaccggcgaa  1501 cagtgggaag ccgggctcaa gcagacgctg ctcgacgggc gcgcgtactg gacggtcgcg  1561 gtgtacgaca tcacgaagcg caacctgctc agcaccgatc cgttcaatcc ggcgctgcgc  1621 cagcaggtcg gccggcagtc gtcgcgcggc gtcgatctga ccggcggcgc gcggctgtcg  1681 cacggctgga cgatcgatgc gaacgtcgcg ttgctgcgtg cgcgctacga cgcattcaac  1741 cagacggtcg gcggcgcgac ggtgtcgcgg gccggcaacg tgccgtcggg cgtgccgcag  1801 cagaccgcga acctgtggct cggctgggca ttcgccgagc gctggcacgt gaatgcgggc  1861 gtgcgttacg tcggcgcgac ctacggcgac gatgcgaatc gcgtgcgggt gccgtcgtat  1921 acggtgttcg atgcgtcgct gcgctggcag ccgacgtcgc gcaccgagct cgcgctgtat  1981 ctgcgcaatc tcgcgaaccg cacgtatgcg gtaacgacgt cgaacggcgg cgagcaatgg  2041 ctgctcggcc cgtcgcgctc ggcggagctc gtcgcgacga tgcgcttcta g  146 1 mattgalaae aaasapddrh alpainvtas sapadpltqp letgsrlgla sldtpasvet  61 vtadtidarg drtvldavtr tagfasaiap gtggtalsvr gfsgqesvmt lldgvrlmpa  121 agtitfpfdt wsvarievlr gpasvlygeg aiggvvnvvp krpqrtrett lqagvgpdga  181 krfafdttga lgprlsyrfy asdarangla eradthttai ggaltfdvsp qltltldydy  241 grqmpatyyg vpaphgvldp slrklnytvg datisyydqw trlsasyrpa pgvtisnqly  301 yltsnrhwrn aesyvldlat grvtrgdyld ighhqrqigd rlsarfdgml fgranrfvvg  361 tefsqitfsg tnnspygget tvpahgfdpg vftspdptvp qfstrarqaa vfaenrlevl  421 prlawvsglr ydhiafsreq aatgagfdkr fanvgwrtgv vfdiapafta yaqyttgaeg  481 vgslvtlsas qmnvrlatge qweaglkqtl ldgraywtva vyditkrnll stdpfnpalr  541 qqvgrqssrg vdltggarls hgwtidanva llrarydafn qtvggatvsr agnvpsgvpq  601 qtanlwlgwa faerwhvnag vryvgatygd danrvrvpsy tvfdaslrwq ptsrtelaly  661 lrnlanrtya vttsnggeqw llgpsrsael vatmrf  707 81 107026796 YP_624307 171 1 ctacagcttc gcgctcacgc gcacccacgc ggtacgcccc ggctccatca ccggcgcgtt  61 cgccggatag ccgaaacccg cgttgccggc gaggttcaga tgctcggtgt aggccttgtt  121 cagtacgttg tcgacgccga ccgagatttg cacggtcttg ctgacgttgt attgcgtatg  181 cagcgacagc acgccgaatc cggcgctcgg gccgaagtcc ttgccgacca cgttgccctc  241 gttcagcgcg tagcgatgct gcggcgcaac gatgcgccac aggccgcccg ccgaccacgc  301 gccgcgcgtg tattcgaggc cgatgcgcgc ctcgagcggc ggcatctgcg gcagcggatc  361 gccgctcgcc acgttgcgcc cccacgcata cgcgagcgac gtctcgacgc gcagcggcgc  421 gaccggccgc cacgacacgc cggcttcgcc gcccatgatc tgcgcgttga cgttggttgc  481 ctgcgtgatc gggcccatca tgccggccgc atagttgaac aggatgaagt cctggacgta  541 gcccgcgtac gccgacaccc atgcatcgaa ccggtcgctc ttgtattgcg cgccgatatc  601 gagctgcgtg gtcttctccg gctgcaccgc cgagaacgcg ttgatcgaac cggccggccc  661 gcgcgtcgcg gagaacagtt cccagtagtc cggataacgc tccgcgtggc cgatccccgc  721 gtaccacgtg acgggtagcg acgcgagatc gcgctcgtag cgcacgaagc cgctcggcag  781 caccttcgtc cgatcgtcgt cgaaagtcgg gttcggcttg ctcatcatca tgccgctctt  841 catcgcgcgc ttgtcgcgtg cgctcgcata gtcgacgcgc gcgccgccga tcacacgcga  901 gacgtcgctc gcataccacg tcagctcgct gaacacgccc gcgctccaca tggtcgcctg  961 ggcatcccac ggctggtcgc ggtagttctg ctgccccatc gacgcacgcg aatcgagccg  1021 gttcgattgc gcatcgacgc ccgtgacgag cttgaaatcg tcgccgaagc ggaacgtcgc  1081 ggcggcacgc gcgccgaccg tgcggcgccg cacgtccgcg gccatgcgca tcggcatgct  1141 gctcgtcggg tcgggctgcc gcaacgtgta gttgtccatc acgtgatcgg cttcgttgta  1201 gtacacgcgc gcctcgatcc ggtcgagcac gtcgccgaga tgccgcttgt cgaacgacag  1261 gccgaacgtc tcgcggcgga aatgcgcgcc atccatcccg cggcccgcgt agcgcgcata  1321 gccgtcgccg gtgccggcgg tcagctcgag tcgcgtatgg tcgtcgggcg tccagccgag  1381 cgctgcatcg gcgttccatt tgtcccactg cgacggcacg gtgttgccgt tgccgtcctt  1441 gtagtcctgc gaatgcgcgt ggttcgcggt cacgcggcca tagatgtccg gcgtgcctgc  1501 ggtcaggtcg atgttctggt cgttgcggcc gaacgacccg ccgacgagac tgccgtcgaa  1561 gcgcatgccg gggcgctcga aacgcggcgt cacgcgctcg aacagcacgg tgccggccga  1621 cgcacccgga ccgtacagca cggtctgcgg ccccttcacg acggtgacct tgtcatagct  1681 ttccggcgcg atgtacgagg tcggcgcatc catccggttc gggcacgcgc cgagcgtcgg  1741 cattccgttc gccaggatgt tcagccgcga accgaacatc ccgcgcagca cggggtcgcc  1801 gttcgtaccg ccgctacgga tcgacgtgaa gccgggaatc gtcttcaggt aatcggcgcc  1861 gtcgctggcg ggcagcggct ggcgcggcgc cttcggatcg gtgacgacga cgagcggcgt  1921 cgacagcggc gacgcgacga cttcgacggg cggcaacagc atcgccgcat cgcccgacgg  1981 cgcaccggcc gccggcgtgg tttcggctgc ggcagcgctc gcggccagcg cgccgacagc  2041 cagcgcagga acggtgagtt tcagtacgcg cggcagaggc cgcgcaccgg cattgcgtgg  2101 cgcccgcggc gcacgcaaca ggaaattggt at  147 1 mtnfllrapr aprnagarpl prvlkltvpa lavgalaasa aaaettpaag apsgdaamll  61 ppvevvaspl stplvvvtdp kaprqplpas dgadylktip gftsirsggt ngdpvlrgmf  121 gsrinilang mptlgacpnr mdaptsyiap esydkvtvvk gpqtvlygpg asagtvlfer  181 vtprferpgm rfdgslvggs fgrndqnidl tagtpdiygr vtanhahsqd ykdgngntvp  241 sqwdkwnada algwtpddht rleltagtgd gyaryagrgm dgahfrretf glsfdkrhlg  301 dvldriearv yyneadhvmd nytlrqpdpt ssmpmrmaad vrrrtvgara aatfrfgddf  361 klvtgvdaqs nrldsrasmg qqnyrdqpwd aqatmwsagv fseltwyasd vsrviggarv  421 dyasardkra mksgmmmskp nptfdddrtk vlpsgfvrye rdlaslpvtw yagighaery  481 pdywelfsat rgpagsinaf savqpekttq ldigaqyksd rfdawvsaya gyvqdfilfn  541 yaagmmgpit qatnvnaqim ggeagvswrp vaplrvetsl ayawgrnvas gdplpqmppl  601 earigleytr gawsagglwr ivapqhryal negnvvgkdf gpsagfgvls lhtqynvskt  661 vqisvgvdnv lnkaytehln lagnagfgyp anapvmepgr tawvrvsakl  708 81 107022434 YP_620761 172 1 atgggtaatg aaagcagccc tagcccatcg tccaccgact ctccgatcat ggcttccatc  61 cgtctcacgt atcagcgccg tccggcgccc gcccgtcatc tgccgcgcgc gtcgcgcccg  121 gccgcgtcgg gccgtcttgc ccgccggctg gcgggtgccg tcctgctgtc ggcactgctg  181 ccgctgcccg cgctggccga caccgacgcc gatgcggcgc ccgccacgca gcgcgcgtcg  241 cgtcgggcgt tcgacattcc ggccggcccg ctcgaagcgg cgctgaaccg gttcggccgc  301 gacgcgggca tcctgctcgc atttccgccc gagttgacgg ccggtctcgc gagcggcggc  361 gtgcacggcc ggttcgacgt cgacggcgcg ttcgaccgcc tgctggccgg cacggggctc  421 gtcgcgctgc gccagccggg cggtggctac acgctgatgc gcgccgatgg ctcggcggcc  481 ggccctgtgg cggccggcgt cgcgccagcc gccgagttgc cgacgatcga cgtgcgctcc  541 agcgccttgc gcgccgaaag ctatcgcgcg ccgaaggaag cgggcgtgct gcgctccgac  601 attccgctgc tcgacaccgc gcaggccgtc aacatcgtgc ccgcgcaggt gctgcgcgac  661 cagcggccgc gcaatctcga cgatgcgctc ggcaacgtga gcggcatcac gcagggcaac  721 acgctcgcgg gcacgcagga cacgatcatg aagcgcggct tcggcggcaa ccgcgacggc  781 tcgatcatgc agaacggcat gccgctcgtg cagggccgcg cgttcaacgc ggcgaccgac  841 agcgtcgagg tgctgaaggg gccgacgtcg ttgctgtacg ggctgatgga cccgggcggc  901 gtcgtcaacg tcgtcaccaa gcagccgcag ctcgtgcgct acaacgtgat ctcgctcggc  961 gcgtcgacgt tcgggcacgg caagaacggc ggcagcgcga cgttcgactc gaccgggccg  1021 gtcggcgatt cgcggctcgc gtaccggctg atcgtcgacc agtcgaacga acagtactgg  1081 cgcaacttcg gcgagtaccg gcagaccttc gtcgcgccgt cgctcgcgtg gtacggccgc  1141 gatacgcagg tcgcggtgtc gtaccagtac cgcaagttcc attcgccgtt cgatcgcggc  1201 accgcgctcg acccgcgcac caatgcgccg ctcgacatcc ccgcacggcg gcgcatcgac  1261 gagccgttca acaacatgga cggcgaatcg cagctcgcgc aactgagcgt cgatcaccag  1321 ttcaacgcgg actggagcgc gcatttcggc tacagctaca accgcgagac ctacgacgcg  1381 aaccagttgc gcacgaccgg cgtcgaccct gtgaagggca cgatgacgcg cagcaacgat  1441 gcgacgcacg gctcgctcag caccgacagc tacgggatcg gctacgtgac cggcaagctg  1501 acgctcgcgg ggatgcggca cgacgtgcag gtcggcttcg ataccgaata ccgccgcatc  1561 tatcgcaagg acctgctgcg tcaggcggtg aagacgccgt tcagttatat cgatccggtg  1621 tacgggctgc tgccgccgtc gagcacggtg tccgcgagcg acagcgacca gaccgacacg  1681 ctgcacgatg catccgcgtt cttccaggat acggtccacc tgaccgacaa gtggatcgtg  1741 tcgggcggcc tccgctacat cacgtacaac caggtggcgg ggcgcggccg gccgttcgtc  1801 gcgaacaccg atctcagcgg ctcgaagtgg ctgccgcgcg cgggcgtcgt ctacaagtgg  1861 accgatgcgt tctcgctgta cggcagctat tcgcagtcgc tgaagccgtc gtcgtcgatc  1921 gcgccgatga cgggctacat catcgacggc gcgacgccgc ccgaggaagc gaccgcatgg  1981 gaagtgggcg gcaagctcgg cttggccggc gggatgaccg gcacgctcgc gctgttcaac  2041 atcgacaaga agaacgtgct cgtgtcgcag tacaacgacg cgacgaagct gaccgactgg  2101 cgcacgtcgg gcaaggcgcg ctcgcgcggc gtcgagctcg acgtctccgg caagctcggc  2161 gagcgcgtga acgtgatcgc gagctacgcg tacatcgacg cgaagacgac cgaggacccg  2221 ctgtacgcgg gcaaccagct gtggaacgtc gcgcgtcaca ccgcgtcgct cgcggccgtc  2281 tacgacttcg gcacggtggc gggcggcgac gatctgcgta tcggcgcgga cgtgcgttac  2341 gtcggcgcgc ggcccggcga ttcggcgaac agcttcacgc tgccgtcgta cgtgctcgcc  2401 gacgcgttcg cgacctacga cacgcggatc ggcaagcaga agctgtcgtt ccagctcaac  2461 gtgaagaacc tgttcaaccg cacgtactac ccgtcgagcg cgaaccgcta cttcgtcgcg  2521 gtcggcgacg cgcgccaggt gtcgctgctg accacgctgc agttctga  148 1 mgnesspsps stdspimasi rltyqrrpap arhlprasrp aasgrlarrl agavllsall  61 plpaladtda daapatqras rrafdipagp leaalnrfgr dagillafpp eltaglasgg  121 vhgrfdvdga fdrllagtgl valrqpgggy tlmradgsaa gpvaagvapa aelptidvrs  181 salraesyra pkeagvirsd iplldtaqav nivpaqvlrd qrprnlddal gnvsgitqgn  241 tlagtqdtim krgfggnrdg simqngmplv qgrafnaatd svevlkgpts llyglmdpgg  301 vvnvvtkqpq lvrynvislg astfghgkng gsatfdstgp vgdsrlayrl ivdqsneqyw  361 rnfgeyrqtf vapslawygr dtqvavsyqy rkfhspfdrg taldprtnap ldiparrrid  421 epfnnmdges qlaqlsvdhq fnadwsahfg ysynretyda nqlrttgvdp vkgtmtrsnd  481 athgslstds ygigyvtgkl tlagmrhdvq vgfdteyrri yrkdllrqav ktpfsyidpv  541 ygllppsstv sasdsdqtdt lhdasaffqd tvhltdkwiv sgglryityn qvagrgrpfv  601 antdlsgskw lpragvvykw tdafslygsy sqslkpsssi apmtgyiidg atppeeataw  661 evggklglag gmtgtlalfn idkknvlvsq yndatkltdw rtsgkarsrg veldvsgklg  721 ervnviasya yidakttedp lyagnqlwnv arhtaslaav ydfgtvaggd dlrigadvry  781 vgarpgdsan sftlpsyvla dafatydtri gkqklsfqln vknlfnrtyy pssanryfva  841 vgdarqvsll ttlqf  709 27 107023374 YP_621701 173 1 atgaacatga aaatcgcgac tcgcctgtcc gtattcgcac tcgccggcgc actgctggca  61 ggctgcgcca cgcagcaagg caacaatacg gccgtcggta ccggcacggg cgcggcgctg  121 ggcgcgggga tcggggcact ggccggtggc ggcaagggcg cggcgatcgg cgcgggcgtc  181 ggcgcactgg tcggtggcgt gacgggttac aactggcagg cgatcaagaa caagctcgcg  241 ccgtcggcag cgcagacggg cacgcaggtc accgagcagc cggacggctc gctgaagctg  301 aacgtgccga gctcggtcac gttcgcgacg aaccagtacg cgatcacgcc ggcgttcacg  361 ccgctgctga acgacctcgc gacgacgctg aaccagaacc cgcaagtcac ggcgtcgatc  421 gtcggctaca cggacagcac gggctcgcag cagctgaacc agacgctgtc gcagaaccgc  481 gcgcaaagcg tcgtgaacgc gctcgtgcag cgcggcgtga atggcggccg tctgtcggcg  541 caaggcatgg gcccgtcgaa cccgatcgcg gacaacgcga ccgaagccgg ccgcgcacag  601 aaccgccgcg tcgagatcta cctgcgcgcg ccgcaacagc atcagtaa  149 1 mnmkiatrls vfalagalla gcatqqgnnt avgtgtgaal gagigalagg gkgaaigagv  61 galvggvtgy nwqaiknkla psaaqtgtqv teqpdgslkl nvpssvtfat nqyaitpaft  121 pllndlattl nqnpqvtasi vgytdstgsq qlnqtlsqnr aqsvvnalvq rgvnggrlsa  181 qgmgpsnpia dnateagraq nrrveiylra pqqhq  710 19 107021874 YP_620201 174 1 ttactgttgg tagacgaggt cggcgcgacg gttctgcgcc cacgatgctt cgtcgtgacc  61 cgttgcctgc ggcttttcct tgccgaggct cacggcttcc atttgcgaat cgttcacgcc  121 gagcagtgcc atcgcacggc ggacggcttc cgcacgcttc tggcccagcg cgaggttgta  181 ctcgctcgtg ccgcgttcgt cggtgttgcc ctggatcagc acgtggcgct gcgggtggct  241 cttcaggtac tgcgcgtgct gctgcatcag cggctggtac tcgtccttca ccgaatagct  301 gtcgaagtcg aagtagatgc tgcgcttcgc gagcgggctg ttcggatcgt tcagcggatc  361 gacgttcact tgcgcgacgt tgtcggcgct cggctgcgtg ctgactgcgc ccgcgttgtt  421 tgccttgtcg tcgagcttca cgcccgactt gcacgctgcg agcgcgctga tcatcatcac  481 ggccagggcc agacgagctt tattcgacat cat  150 1 mmsnkarlal avmmisalaa cksgvklddk annagavstq psadnvaqvn vdplndpnsp  61 lakrsiyfdf dsysvkdeyq plmqqhaqyl kshpqrhvli qgntdergts eynlalgqkr  121 aeavrramal lgvndsqmea vslgkekpqa tghdeaswaq nrradlvyqq  711 24 107022117 YP_620444 175 1 ttagtttgcc ggaacggtcg tcttctgcac ttgctgcgta ccaaccactt cgacttccac  61 gcggcggtcc ggtgcgaggc aggcgatgag ttgcttgcgg ttcttctggt tgcagccagt  121 cgtaaccggg ttgcgcttgc ccttgccttc cgtgtagatc ttgttggccg gcacaccctt  181 gctgaccagg tacgacttca cggcttgcgc acggcgcagc gacagacggt cgttgtactt  241 gtccgaaccg atacggtccg tgtagcccgt tgcgacgacc acttccgtgt tcatgccttc  301 gatcttcgaa gccagttcgt ccagcttttg cttgcccagc ggcttgagcg ttgccttgtc  361 gaagtcgaac agtgcgtcag cttgatacgt gatcttctgg ctcgtgatgg ccggagcgac  421 cggagcgacc ggcggctgcg gtgcctgggc gaccagtgcg ccatcgcact tcgcgttggc  481 ggtggccggc gtccagaacg catcgcgcca gcagagctcg ttcgtgccgt tcatccacac  541 ccattcgccc gtgccgttca cccagttgtc attgacggct tgacgcgacg ccggcaccga  601 ctgtgccgaa gcggatgcag ccataactgc ggtagctgca atgaacgcga gctttgaaag  661 tttattcat  151 1 mnklsklafi aatavmaasa saqsvpasrq avndnwvngt gewvwmngtn elcwrdafwt  61 patanakcdg alvaqapqpp vapvapaits qkityqadal fdfdkatlkp lgkqkldela  121 skiegmntev vvatgytdri gsdkyndrls lrraqavksy lvskgvpank iytegkgkrn  181 pvttgcnqkn rkqliaclap drrvevevvg tqqvqkttvp an  712 40 107022082 YP_620409 176 1 tcagaagcga tggcgcaggc cggcggtcac cgcgatctgc ttgttggtcg acgaagcgcc  61 accgaggcca ttgacatagg cgccgatgcc caggccgtcc gtgctgactc gctgatacac  121 gccttgcaca tacacgtcgg tccgcttgga cagcgcatag tcggtctgca ggttgaactg  181 attccagccc ggatgacggc cgtccaggaa gccggcggtg tacgtgtacg acccggccag  241 cgacaaggcc ggcgtgagcg cgtagcgcgc gttcacttcg tagttgttga agcgggcgaa  301 cgtgccgtcg agacccaggc cgctcgacac gcccgaggcg cccgcggaga tgccgagcgc  361 ccggttcaca cgcgtttgcg tgaatacgaa gccggccgtg gcggggccga acgtgtagtt  421 caggccgccg ccgaacacgc gctgacgctt gccgacgaac gtgttgtcgc ctgccaccgc  481 gccggcgctg ttggtgaccg cagctgccgt gagccccgag gtgtcattgt tcaactgcag  541 gtagccggcg ccgatgttga agccggcgta ggtgtacgac acccccgcgc tgtatgcacg  601 gttgttcgag aactggttgc tgttcgagaa gccgtacaac gcgccgaact tcaggccgcc  661 gaaattgacg ctcgtatatt tgaccgagtt attgatccgg aacgaattgt tcaggttgtc  721 gttgtcgaac gggtgggcaa attgagtgcc gccgaattgc gtgccggtca gcgacagcgg  781 cccgatgtaa tcgacgacgc tatcgtattg gcgaccgagg gtgagggagc caaactggtc  841 ttgcgacagg ccgacgaacg cctggcggcc gaactcgcgg ccattttggc ccagcgtgcc  901 gttggcgatg ttgaagccgt tctccaacac gaagatggct ttcagtccgc cgccgagatc  961 ttccgtgccg cgcaggcccc agcggctgcc gttgatctgg ccgctgcgct ccgaccatga  1021 gctatgcccg ccctgattgt tcgtgtaggt gatgcctgca tcgatcaggc cgtacagcgt  1081 aacgctgctt tgagcatggg ctgcggtaac gaatacaccc gacaa  152 1 msgvfvtaah aqssvtlygl idagitytnn qgghsswser sgqingsrwg lrgtedlggg  61 lkaifvleng fniangtlgq ngrefgrqaf vglsqdqfgs ltlgrqydsv vdyigplslt  121 gtqfggtqfa hpfdndnlnn sfrinnsvky tsvnfgglkf galygfsnsn qfsnnraysa  181 gvsytyagfn igagylqlnn dtsgltaaav tnsagavagd ntfvgkrqrv fggglnytfg  241 patagfvftq trvnralgis agasgvssgl gldgtfarfn nyevnaryal tpalslagsy  301 tytagfldgr hpgwnqfnlq tdyalskrtd vyvqgvyqrv stdglgigay vnglggasst  361 nkqiavtagl rhrf  713 44 107026115 YP_623626 177 1 atgaagcacc ccgtcatttt tgccgccgcg ctcgctgcct tcgccgcgcc ggcctttgcc  61 gagaccagcg tgacgctgta cggcgtgatc gacgaaggct tcaattacac gaacaacgtc  121 aacgtcaatg gggtcggaaa gaccaattac cagctcgcga gcggctatgc gcagggcagc  181 cgctggggcc tgaagggcac cgaggatctc ggcggcgggc tgaaggcggt cttcacgctg  241 gaaaacggtt tcgacgtgaa taacggccgg ctcggccagg gcggccgcat gttcggccgt  301 caggcgttcg tcgggctgtc cgagtcgcgc ttcggcacgc tgaccttcgg ccgccagtac  361 gatgcggtcg tcgactatct cgcgccgctg accgcgaacg gcaactgggg cggcacgctg  421 ttctcgcacc cgttcgacaa cgacaacacg gacaactcgt tccgcgtcaa caacacggtc  481 aagtacgcga gcgccgactg gaacggcctg acgttcggcg gcacgtacag cttcagcaac  541 agcacgggct tctcgaacaa ccgccagtac agcatcggcg cgcagtattc gctggccggg  601 ctgcaggtcg cggccgcgta cctgcaggcg aacaacccgg gcatcggcag cgcgggcgcg  661 atcgcggccg acgacgcgaa cttcgtcgcc gaccggctgc gcatcttcgg cggcggcgtc  721 aattacacgt tcggcccggc gacggtcggc gtcgtctaca cgaagaccga cgtgaagaac  781 ccggtatcga cggtctacct gccggcgtcg acgttcgccg gtctcgggct gaccgcgacg  841 aagttccaga acttcgaaat caacggcaag taccagctca cgcccgattt ctatctcggt  901 gcgcaatacg tatacacgga cggcaagtac gatgcggcgg ccggttcgtt caagccgaag  961 taccacacgg tcggcctgat ggccgactac agcctgtcga agcgcaccga cgtgtacctg  1021 cagggcgcgt ggcagaaggt gggcggcgac aagacgggca cggcggccga cggcggctat  1081 gtcgtcggga cggacggccc gtcggcgtcg tcgaaccagt tctcggtgcg cgccgcgatt  1141 cgccacaagt tctga  153 1 mkhpvifaaa laafaapafa etsvtlygvi degfnytnnv nvngvgktny qlasgyaqgs  61 rwg1kgtedl ggglkavftl engfdvnngr lgqggrmfgr qafvglsesr fgtltfgrqy  121 davvdylapl tangnwggtl fshpfdndnt dnsfrvnntv kyasadwngl tfggtysfsn  181 stgfsnnrqy sigaqyslag lqvaaaylqa nnpgigsaga iaaddanfva drlrifgggv  241 nytfgpatvg vvytktdvkn pvstvylpas tfaglgltat kfqnfeingk yqltpdfylg  301 aqyvytdgky daaagsfkpk yhtvglmady slskrtdvyl qgawqkvggd ktgtaadggy  361 vvgtdgpsas snqfsvraai rhkf  714 39 107025986 YP_623497 178 1 atgaacaaga ctctgatcgt tgcagcagct gcagcatcgt ttgctaccgt cgctcacgcg  61 caaagcagcg tcacgctgta cggcgtgctg gacgcaggca tcacctacca aagcaacgtc  121 ggcggcaagt cgctgtggtc gatgggctcg ggcatcgacc aaagccgttt cggtctgcgt  181 ggttcggaag acctcggtgg cggcctgaag gcaatcttca cgttggaaag cggcttcaac  241 atcggtaacg gccgcttcgc aaacggcaac ggcggcatgt tcaaccgcca agctttcgtc  301 ggcctgtcga gccagtacgg cacggtcacg ctgggtaagc agtatgacgc aacgcaagac  361 tacctggcgc cgctgacggc aacgggctcg tggggcggca cgtacttcgc gcacccgctg  421 aacaacgacc gtctgagcac gaacggcgac gtcgcgctga acaactcgat caagtacacg  481 agcgctaact acgctggcct gcaattcggc ggtacgtact cgttctcgaa caacacgaac  541 ttcggcaaca accgcgcata cagcggcggt ctgtcgtacc agttccaagg tctgaagctg  601 ggtgcagcat actcgcaagc aaacctgggt gacggtacca acacgaacgg cgcatcgacg  661 ctgggtggcc aaggccgtgt ccgtacgtac ggtgctgctg ctggttacgc attcggcccg  721 gcacaagtcg gcgctgcatg gacgcaatcg cgtatcgaca accaagctgc tggcgttccg  781 acgctgcgcg ctgacaacta cgaagtcaac gcaaagtaca acctgacgcc ggctctcggc  841 ctgggtgctg cttacacgta cacgaacgcg aaggtcaaca acggcagctc gcactggaac  901 cagttcggcg tgcaagctga ctacgcgctg tcgaagcgca ccgacgtgta cgcacaagct  961 gtgtaccagc gtggcgcgaa gggcaacaac atcgttggca cgggcatcta caacggcgac  1021 aacacgacgg catcgagctc gtcggtcaac caaaccgcag caacggttgg tctgcgtcac  1081 cgcttctaa  154 1 mnktlivaaa aasfatvaha qssvtlygvl dagityqsnv ggkslwsmgs gidqsrfglr  61 gsedlggglk aiftlesgfn igngrfangn ggmfnrqafv glssqygtvt lgkqydatqd  121 ylapltatgs wggtyfahpl nndrlstngd valnnsikyt sanyaglqfg gtysfsnntn  181 fgnnraysgg lsyqfqglkl gaaysqanlg dgtntngast lggqgrvrty gaaagyafgp  241 aqvgaawtqs ridnqaagvp tlradnyevn akynltpalg lgaaytytna kvnngsshwn  301 qfgvqadyal skrtdvyaqa vyqrgakgnn ivgtgiyngd nttassssvn qtaatvglrh  361 rf  715 55 107026730 YP_624241 179 1 atgaagaaac ttgctctctc gaccctctcg ctcgccctgc tgggcgcggc cggcgctgct  61 caagctcagt cgagcgtcac gctgtacggc gtgatcgaca cgtcgatcac ctatgttcac  121 ggtaacgatg gtcaaggtaa caattcgtgg tcgatgggta gcggcaacct gcaaggcagc  181 cgcttcggcc tgaagggttc ggaagacctc ggtggcggcc tgaaggcgat cttccagttg  241 gaaaacggct tcaactcggc atcgggcgca ctgggccagg gtggccgcat gttcggtcgt  301 caagccttcg tcggcctgca aagcgaccag tacggtacgc tgacgctggg tcgccagtac  361 gacccgctgg tcgacctggt ccaggcagtc acggctgaca actacttcgg cagcgtgttc  421 gccacgccgg gtgacgtcga caacaacgac aacagcctgc gcgtcagcaa cgcgatcaag  481 tacacgtcgc cggtctgggc aggcctccag gtcgaagcgc tgtacgcact cggcggcgtc  541 gccggctcga ccggcaaggg tcagacgtgg gcggcagcgg cagcgtacaa caacggcccg  601 ctcggcctgg ctgcaggtta cttccacgcg aacaactcgg ctccgctgag cgcagttggc  661 cagcgcaccg gctgggccgg cacgtcggac gcgatcttcg acggctcggg taacttcatc  721 aacaacgcgt acacgtcggc atcgtcgatc ggcatcgcgc aagttgctgg ccagtacgca  781 ttcggcccgg tgacgttcgg cctcggctac agcaacgcgc aatacaaggc ggatgcgaac  841 tcgggcttcg gcacgaacca gaagtacaac acgggtcgcg cattcgtcac gtaccaggca  901 tcggcaccgc tgctgctggg cgtcggccac atctacacga agggcagcgg ccagacggac  961 gccaagtacc accaggtttc gctcggcgcg gactactcgc tgtcgaagcg tacggacatc  1021 tacctggttg gcgcgtacca gcacgcaagc ggcaagaacg ccgacggtac cgatgcgcaa  1081 gcgtcggtcg gctcgtacgg catcgccggc aagagctcgc aggaaatcgt cgcactcggc  1141 ctgcgtcaca agttctaa  155 1 mkklalstls lallgaagaa qaqssvtlyg vidtsityvh gndgqgnnsw smgsgnlqgs  61 rfglkgsedl ggglkaifql engfnsasga lgqggrmfgr qafvglqsdq ygtltlgrqy  121 dplvdlvqav tadnyfgsvf atpgdvdnnd nslrvsnaik ytspvwaglq vealyalggv  181 agstgkgqtw aaaaaynngp lglaagyfha nnsaplsavg qrtgwagtsd aifdgsgnfi  241 nnaytsassi giaqvagqya fgpvtfglgy snaqykadan sgfgtnqkyn tgrafvtyqa  301 saplllgvgh iytkgsgqtd akyhqvslga dyslskrtdi ylvgayqhas gknadgtdaq  361 asvgsygiag kssqeivalg lrhkf  716 58 107022697 YP_621024 180 1 atgtttgcgc tgaatgcacg tggcgcgctg cgggcaccgc tcgcgctggc cgccgcgctc  61 gcgctcgccg ggtgttcgct cgcgccgcgt tacgagcgtc cggccgcgcc ggtgccggcg  121 acctatacgc cggtcgacgg cggcacgacg ccggccgccg aaccggccac cgcgcaggat  181 gccgcgctgc tcgacgactg gcatgcgtat ttcaccgatc cggcgctgca ggcgtggatc  241 gacgcggcgc tcgcgaacaa ccgcgaccta cggatcgccg ccggccggct cgaggaggca  301 cgtgcgctgt acggcgtgca gcgcgcggac cggatgccgt cggtcgatgc gaatctcggc  361 tatgaacgcg cgcgccagta cgacccggtc gtgcgcgaaa gcgcgatcag cgggctgtat  421 cgcgcgggtg tcggcgtcag cgcgtacgag ctcgacctgt tcggccgcgt gcgcagcctg  481 tccgaggcgg cgctcgccga ctatttcgcg acggccgatg cgcagcgcac ggtccgcatc  541 ggcgtgatcg ccgaagtggc gggcgcatac gtttccgaac gatcgttgca cgagcaactg  601 gcgctcgccc agcgcacgct cgatgcgcgc gagcggatgg ccgcgctcac gcaacgccgc  661 tatgccgccg gcacgagcga cgcgatcgag ctgcgttcgg ccgagatgct ggtggcgtcc  721 gcgcgcgcgt cgcaagccgc gctgcagcgc gagcatgcgc aggccgtccg cgcgctgcaa  781 ctgctggcgg gcgatttcgc gcgcaacgtg cccgacgacg cgaccgcgct tgacacgctg  841 tcgatcgcgc ccgtggcgcc gggtgcgccg agccagttgc tcgaacggcg gccggacatc  901 cggcaggccg aggggcggct gaaggccgcg aacgcgaaca tcggcgcggc gcgcgcggcg  961 ttcttcccgc gcatcgcgtt gacgaccgac tacggctcgg tgagcgacgc gttctcgaac  1021 ctgttcgcgg ccggcacgag cgtatggacg ttcgcaccgc gcatcacgct gccgatcttc  1081 gcgggcggac gcaatcgcgc gaacctcgac gttgcgcacg cgcgcaagga cattgcggtc  1141 gccgaatacg agaaggcagt gcagaccgcg tttcgcgaag tggccgatgc gttcgccgcg  1201 cgcgactgga tcgaccggca gctgaccgcg cagcaggacg tgtatgcggc ggacggcgcg  1261 cggctgaagc tcgcggagcg tcgttatgcg ggtggtgtcg cgacctatct cgaactgctc  1321 gacgcgcagc gcagcacgta cgagtcgggg caggagctga tccggctcag gcagctcagg  1381 ctggcgaatg cgatcgcgct gtatcgcgcg ctcggcggcg gctgggcgcc ggccacggcg  1441 gaggccgcgg cttccgcgtg a  156 1 mfalnargal raplalaaal alagcslapr yerpaapvpa tytpvdggtt paaepataqd  61 aallddwhay ftdpalqawi daalannrdl riaagrleea ralygvqrad rmpsvdanlg  121 yerarqydpv vresaisgly ragvgvsaye ldlfgrvrsl seaaladyfa tadaqrtvri  181 gviaevagay vserslheql alaqrtldar ermaaltqrr yaagtsdaie lrsaemlvas  241 arasqaalqr ehaqavralq llagdfarnv pddataldtl siapvapgap sqllerrpdi  301 rqaegrlkaa nanigaaraa ffprialttd ygsvsdafsn lfaagtsvwt fapritlpif  361 aggrnranld vaharkdiav aeyekavqta frevadafaa rdwidrqlta qqdvyaadga  421 rlklaerrya ggvatylell daqrstyesg qelirlrqlr lanaialyra lgggwapata  481 eaaasa  717 55 107023544 YP_621871 181 1 ttacccgttc gtcgccgttg ccgacgcggg cgcaggagcc gccgccttgt cgtagtcgac  61 cggcgcatcc ggcgcgcgcg gcgcttcgcc ggcatgctgg atccagccgc cgccgagcgc  121 gcggtacagg tcgaccaggt tcgtccagcg cgccaggcgt gcgctgatca gcgactgctg  181 cgccgagtac agatccgtct gcgcagtcag caccgacagg tagctgtcga caccgttctt  241 gtaacgcagg tccgacaggt cgaagcgacg ttgctgcgcg tgctcgttgc gctcgagcgc  301 cgcgatctgc tggtcgtacg tgccgcgtgc ggcgaggcca tccgacactt cgcggaatgc  361 cgactggatc gccttctcgt agttcgcgat ctcgatgcgc ttctgcacgt gcgcgagatc  421 gaggttcgcg atgttctgcc cgccctcgaa gatcggcagt gcgatgctcg gcgcgaacga  481 ccatgccgcc gtgccggcct tgaacaggcc gcccagcgtc gggctcgcgg tgccgaacgc  541 gcccgtcagc gaaatcttcg ggaagaacgc cgcgcgcgcc gcgccgatgt tcgcgttcgc  601 ggccagcagc gtctgctcgg cctgcatcac gtcaggacga cgcgtcagca gatccgacgg  661 caggccagcg ggcacgtccg tcagcaggtt ctgcgcatcg agcggcatgc ccgccggcag  721 gtcgtccggc agcggctcgc cgatcagcag caccagcgcg ttcagcgcct gcgcgcgggc  781 acgggcctgc gcctgctggt tcgcgagcgc cgtctcgacg accgtctgcg cctgacgcag  841 ctcgagctcc gagcccgtac cgttgtcgaa ctgcagcttg gtcaggtcgt acgatgcctg  901 cgcggtcttc agcgtgtcct ccgtgacctt cagcaggtcg tcggtcgaca gcagcgtcag  961 gtattgatcc gccacctgcg acaccagcga gatctccgac gcctgccgcg catacgcggt  1021 cgacaggtat tgcgcgagcg cctgatcctt caggctctgc acgcggccga acaggtcgag  1081 ctcccacgac gcggacaatc caacgttata ggtgcgcgaa atcagcggcg ccctcgtcgt  1141 cgacacaccg gccggcagac gctggatgtt gcccgtgccc gtaccgtcga gcgtcgggaa  1201 cagccccgca cgcgtgatct ggtactgagc acgcgccgcc tcgatattga gcaccgacac  1261 gcgcaggtcg cggttgtttt tcagcgcgat ctcgatcagc cgctgcaggc gcggatcgac  1321 gaagaactcg cgccagccga tggcagtcgc cgcctggccg ttcgcgctgc gcgcgccggc  1381 tgcgcccggc tgcgtcgcgt agacgccgcc ggccgggtac gcctgcgcga cgggtgcgtc  1441 gggccgcttg tagtgcggcg ccatcgtgca gcccgtggca aagagcgcga ctgcgattgc  1501 agtcaaagcg tgtttttgca tcat   157 1 mmqkhaltai avalfatgct maphykrpda pvaqaypagg vyatqpgaag arsangqaat  61 aigwreffvd prlqrlieia lknnrdlrvs vlnieaaraq yqitraglfp tldgtgtgni  121 qrlpagvstt raplisrtyn vglsasweld lfgrvqslkd qalaqylsta yarqaseisl  181 vsqvadqylt llstddllkv tedtlktaqa sydltklqfd ngtgselelr qaqtvvetal  241 anqqaqarar aqalnalvll igeplpddlp agmpldaqnl ltdvpaglps dlltrrpdvm  301 qaeqtllaan anigaaraaf fpkisltgaf gtasptlggl fkagtaawsf apsialpife  361 ggqnianldl ahvqkrieia nyekaiqsaf revsdglaar gtydqqiaal ernehaqqrr  421 fdlsdlrykn gvdsylsvlt aqtdlysaqq slisarlarw tnlvdlyral gggwiqhage  481 aprapdapvd ydkaaapapa satatng  718 19 107028625 YP_625720 182 1 ttactcggcc gaacccatca tc tttgctg atagttctgc aggccgacct tgccgatcag  61 gtcgatctgc gtttcgagcc agtcgatgtg ctcttcggtg tcgtcgagga tcttctcgaa  121 gatctcgcgc gacacgtagt cgcgtaccga ttcgcagtag gcgatcgctt ccttgcaggt  181 ggactgcgag atctgctcga gcttcaggtc gcacttcagg atctcttcgg tttcctcgcc  241 gacgagcagc ttgtgcaggt cctgcaggtt cggcaggccg tcgagcatga acacgcgctc  301 gatcagccag tcggcgtgct tcatttcgcc gatcgactcg tcgtattcgt gcttgccgag  361 cttgtcgagg ccccagtgct tgtacatgcg cgcatgcagg aagtactggt tgatcgccgt  421 gagttcattc ttcagttggg cgttcagata ttcgatgact ttcttgtcgc cttgcat  158 1 mqgdkkviey lnaqlknelt ainqyflhar mykhwgldkl gkheydesig emkhadwlie  61 rvfmldglpn lqdlhkllvg eeteeilkcd lkleqisqst ckeaiayces vrdyvsreif  121 ekilddteeh idwletqidl igkvglqnyq qtmmgsae  719 8.5 107028623 YP_625718 183 1 tcaggccgcc ttgcggtcgt agaacgtgac cgggatcgga tgagcatgat gctcgacacc  61 gcaccggctc gcgcagacgc cttgctcggc catgaggtcg cgtacggact cctcgcactt  121 gccgcagcac gtggccacgc cgagctcgaa ctggagttca tcgaaagagt tcacgccctc  181 ggcgagggac gcgcgaatct tgcgatcgga aacagacttg cacacgcaga cgatcat  159 1 mivcvcksvs drkiraslae gvnsfdelqf elgvatccgk ceesvrdlma eqgvcasrcg  61 vehhahpipv tfydrkaa  720 36 107026550 YP_624061 184 1 tcatgctggc ctcacttgaa ccggatggtc gggttgatga ccgcgtacag cacgtcgacg  61 gtcaggttga tcaggatgaa ttccagcgag aacagcagca cgatcgcctg gatcaccggg  121 tagtcgcgca tcgtcaccgc atcgaccagc aggcggccga gccccggcca gttgaacacg  181 gcctcgacga cgatcgagcc gccgagcagg aagccgaact gcagccccca tcatcgtgac  241 gaccggaatc atcgcgttgc gcaggcaatg cttgagcacg accatcggct cgtgcacgcc  301 cttcgcgcgc gcggtgcgca cgaagtcctc gttcagcacc tcgacgaacg acgcgcgcgt  361 gaagcgcgcc atcacggcgg cgaccgcggc gccgagcgtc agcgacggca gcacgtagct  421 cttccacgtg ccgtccggca cgaccggcag ccagccgagc ttcaccgaga agatctccat  481 cagcagcatg ccgagcgcga acgcgggaaa cgaaataccc gacaccgcga tcgtcatccc  541 gagtcggtcg ggccagcgat tgcgccacac ggccgacgcg atcccgatcg ccatcccgat  601 cgccgtcgcc cacaccatgc tgacgagcgt gagcagcagc gtcggcatga agcgctcccc  661 gatctcggtc gacaccggcc gcttgctgcg cgtcgagatg ccgaaatcgc cgtgcgcgat  721 cttcacgaag aagttcgcga actgggtcgg catcggcttg tcgagcccaa gatcggcacg  781 cacgagcgcg accgtcgcgt cgtcggcttc ggggccggcc gcgagccgcg ccgggtcgcc  841 gggcagcagg tgcacgaaca ggaacaccag caccgcgacg atcgcgagcg tgggcagcag  901 gccgaacagg cgtttgacga ggaaattcag cat  160 1 mlnflykrlf gllptlaiva vlvflfvhll pgdparlaag peaddatval yradlgldkp  61 mptqfanffv kiahgdfgis trskrpvste igerfmptll ltlvsmvwat aigmaigias  121 avwrnrwpdr lgmtiavsgi sfpafalgml lmeifsvklg wlpvvpdgtw ksyylpsltl 181 gaavaavmar ftrasfvevl nedfvrtara kgvhepmvvl khclrnamip vvtmmgaavr  241 lparrldrrr grvqlagarp pagrcgddar lpgdpgdraa vlagihpdqp drrravrghq  301 pdhpyqvrpa  721 42 107026454 YP_623965 185 1 ttagaagcgg tggatcatgc cgacgccgac gccgatctgg ttctgcttcg aacctgcgcc  61 cacgccgtca ccgatcgacg tggtcgccga gatgattgcg ttgccgcggc cgttcagcgt  121 gttgccgctt gcgtgctggt aagcctcaac cgcgtacagg cccgtgcgct tcgacaggct  181 gtagtactgc gacagcgtga cctggtggta cttggccgag ctcgtgatgc cgttcgactg  241 cgttgcggcc gtgtacgagt agcctgccgc gaagtcccac tgagcagctg ccttccagtg  301 cagcacggcg ccagccgtgt tgaagattgc cttgttccgg aagaacgaac ccgtgcccgg  361 cgtgtactgg acgttcgagt acgacgccga aatatcccat gccggcgtga actggtagcc  421 cgccgtcacg gcgatacgct gctgcgattg cgcggtcgcg tagccgttgt tgatcgacga  481 gactgccggc tggttgccgt cgcccgctgc cgtcaggccg ttctggaccg tcgagttcgc  541 gccccacacg ccgccgccga gcgtcgagtt gttgaccttc tgatagccga ccgcgatgcc  601 tgccgggccg ttcaggtact ggatcgccgc gctccacgtc gagccgcggt tcgtcgcgcc  661 tgcgacgccg ccgaacgagt acgaaccgcc gaccgtgaag ccgtagaact tcggcgacat  721 gtagacgagc gagttgttcg cgcggtagct ggtatccagc gaatcgatat cgcccgggtg  781 cgcgccgtag tagccggtca gccaggtcgt cgggctgtac ggcgacagca gcgtgtagta  841 cgcggtgtac tggcggccgg ccgtcagcgt accgtacgtg gcgttggtca ggccaaccca  901 tgcctgacgc gtgaagatgc cgttggtcca ctgcgacgag ccgttagccg tgttgacgcc  961 ggcttccaac tggaaaatcg ccttcgtgcc gccgccgaga tcttcgctgc ctttcaggcc  1021 gaagcggctg cctgcccaca cgccggtgga catctgcacc ttcgagtgac cacccgaggt  1081 tgcgccgact gccgacgagt t ttctgcca agcgataccg ttgtcgacga taccgtagag  1141 ggtcacgctg ctctgggcgt gggcggcggt agcggctgcg agaccgactg ccgtcatggc  1201 gaaagcgacg cgctttttca t  161 1 mkkrvafamt avglaaataa haqssvtlyg ivdngiawqn nssavgatsg ghskvqmstg  61 vwagsrfglk gsedlgggtk aifqleagvn tangssqwtn giftrqawvg ltnatygtlt  121 agrqytayyt llspyspttw ltgyygahpg didsldtsyr annslvymsp kfygftvggs  181 ysfggvagat nrgstwsaai qylngpagia vgyqkvnnst lgggvwgans tvqngltaag  241 dgnqpavssi nngyataqsq qriavtagyq ftpawdisas ysnvqytpgt gsffrnkaif  301 ntagavlhwk aaaqwdfaag ysytaatqsn gitssakyhq vtlsqyysls krtglyavea  361 yqhasgntln grgnaiisat tsigdgvgag skqnqigvgv gmihrf  723 85 107022089 YP_620416 186 1 atgtcgttca cctttgccgc gcggccctcg cgcggccggc tcgcgctcgc ctgcgcggcc  61 gctttcgcat ggccggccgc ttacgcggct gccaccgacg atgtccgcgc cgggaccgcc  121 cgcggcggtg gcgtcgatcg tcccccgacc actgcgaccg ccgcgccggc tgccgccggc  181 gtcgcggcag cgtccgcacc ggccggcgat acgctgagcg ccgtcagcgt gaccgcgcag  241 cggcaaccgg tcgacccgga tacgccggcc gtcgtcacgt caattacgcg cgagcagatc  301 gatgcgcata ccaacgtcac caccgaggac gcgctgaagt acgcgccgaa cctgatggtc  361 cgcaagcgct acatcggtga ccgcaacagc atattcgcgg gtcgcgactt caacgaactg  421 cagagcgcgc gcgggctcgt ctatgcggac ggcgtgttgc tgtcgaacct gctcgggtcg  481 agctatgcat acccgccgcg ctggtcgctg attccgcccg acgacatcgc gcgcgtcgac  541 gtgctctatg gccctttttc cgcgctctat ccgggcaacg cgatcggctc cactgtgctg  601 ctcaccacgc gccgcccgga acaactcgag gcgtcgctgt cgacgcagtt ctttacgcag  661 cgctaccacg acggctacgg ctttgccgac agcttcggcg gcaatcacca gacggcgcgg  721 atcgcgaacc gcgtcggccg gttctggttc gcgttgtcgc tcgaccggct ggagaacaac  781 ggccagccga tgcagtacgc gggccccaat tcgacctaca acccgaaact gggcgccgcc  841 gtgccggtga cgggcgccgc gaccgacatc ggccccaacg gcaagccgcg gaccatcgtc  901 ggcgcgcaga cgatcgagcg gaccgaacag ctcaacgaga cggtccggat gggctatgcg  961 ttcaccgatc acgtcgatgc gacgctgacg ctggggcatt gggagaacca ttaccggcag  1021 cacggcgaga ccttcctgcg agatgcggcg ggcaatcccg tctacggcgg caacgtgtcg  1081 atcggcggcc agaacatgac ggtcgcgccg aacgcgttcg cgccgcagcg cggcgaccag  1141 gagaactggc tgtacgcgct cgggttgaac ggccggctcg attccggctg gcggctgtca  1201 ggcgtcgtgt cggcgtacga cgtgtcgcgc gacgtgctgc gcgcggcctc gaccgtgcag  1261 ggtggggcgg gcacgctgtt ccagggcgac ggcaccggct ggcgcacgct cgatctgaag  1321 gccgaggcgc cggtagtgaa aggtcatacg ttcacgttcg gctatcacta cgacaactac  1381 ttcctgcgca acgtcacgta caacacggcc gactggctgg ccggcccgac cacgtcgctc  1441 gcgagcgtct atcgcggcga cacgcgcacg caggcgctgt tcgggcagga cgcgtggcgt  1501 ttcgcgccgc gctggctcgc gacgctcggg ctgcgctacg agcgctggga tgcgtatggc  1561 ggcgcgctcg gcaatgcgcg cggcacgctc ggctatgcgg accgcggcgc gaacgcgctg  1621 tcgccgaaag tcgcgctgca gtgggacgcg acggaggtct ggcgcttccg gctgtcgttc  1681 gcgaccggca cgcggttccc gacggtcggc gaattgttcc agggcacgat ctcgaacaac  1741 gcgatcgtca acaacaaccc gaacctgcgg ccggagaagg cgatcgactg ggacttcacg  1801 gccgaacgcg acgtgggcgt cggcgtcgtg cgcgccagcg tattccagag cgatctgcgc  1861 gactcgatct acagccagac gacggtgtcg ggcgcgacca ccgtcaccaa catctcgaac  1921 gtcgaccgcg tgcgcgtgcg cggcgtcgaa ctcgcgttca gcggcgagaa cgtcggggtg  1981 cgtgggctcg atctcgacgc gaacgtatcg gcgagcaacg cgcagatcct cgccgatgcc  2041 gcgaacccgg cctacgtcgg ttcgcgtttc ccgcgcattc ctcgcatgcg cgcgaacctg  2101 ctcgcgtcgt atcgcttcga cgagcactgg ctcacgagcg tcggcgtgcg ctactcgggc  2161 cgccagttca acacgctcga caacagcgac gtgaatccgg acgtctacgg cggcacgagt  2221 tcgttcacgg tcgtcgatct gaaggcgcgc tatcgcttcg atcgtcactg gaccgcgtcg  2281 gccggcatcg acaacgtgac ggaccgccgc tactacacgt tccacccgta tccgggccgc  2341 acgttctatg gagaactgaa atggtcgctg tga  162 1 msftfaarps rgrlalacaa afawpaayaa atddvragta rgggvdrppt tataapaaag  61 vaaasapagd tlsavsvtaq rqpvdpdtpa vvtsitreqi dahtnvtted alkyapnlmv  121 rkryigdrns ifagrdfnel qsarglvyad gvllsnllgs syaypprwsl ippddiarvd  181 vlygpfsaly pgnaigstvl lttrrpeqle aslstqfftq ryhdgygfad sfggnhqtar  241 ianrvgrfwf alsldrlenn gqpmqyagpn stynpklgaa vpvtgaatdi gpngkprtiv  301 gaqtierteq lnetvrmgya ftdhvdatlt lghwenhyrq hgetflrdaa gnpvyggnvs  361 iggqnmtvap nafapqrgdq enwlyalgln grldsgwrls gvvsaydvsr dvlraastvq  421 ggagtlfqgd gtgwrtldlk aeapvvkght ftfgyhydny flrnvtynta dwlagpttsl  481 asvyrgdtrt qalfgqdawr faprwlatlg lryerwdayg galgnargtl gyadrganal  541 spkvalqwda tevwrfrlsf atgtrfptvg elfqgtisnn aivnnnpnlr pekaidwdft  601 aerdvgvgvv rasvfqsdlr dsiysqttvs gattvtnisn vdrvrvrgve lafsgenvgv  661 rgldldanvs asnaqilada anpayvgsrf priprmranl lasyrfdehw ltsvgvrysg  721 rqfntldnsd vnpdvyggts sftvvdlkar yrfdrhwtas agidnvtdrr yytfhpypgr  781 tfygelkwsl  724 78 107026278 YP_623789 187 1 tcagaacgtg gtgcgcaatc cggccatcag gcttcggccg ccttgcgggg cgaagccgcg  61 cacgaccgac gtcgagtagc gaatctcctg gttcgtcagg ttgtcgccgc gcaggtgggc  121 gagccagtgc gtcgggccga cgcggaattt gtacgtcagc atcacgccga gcgacgtata  181 gccgtccgtc gagaagtcgt cgtcgggcac gcgatgctgc gaccacgcgt gcgtgacctg  241 tgcgcgcgca ccgaacgggc cgtaaccgta gtcggccgcg agcgtcgcgc gcagcggcgc  301 gatgcgcggc agcggctgcc ccgtgtcgac gttgcgcgcg tgcgtgtagt cggccgtcag  361 ttcgagatcg accgtgtggc cgcgccgcga gaacgcacgc cacttgccgt ccagctcgac  421 gccgtagaac tcggcgcgca cgccgcggta gatcgcctcg ttgagcgagc cgtcagtgcc  481 gggcgcgacg ggctcgccgt cgtcgtccac cacgcggccg gtgttgtact cggtcagata  541 gttcgagaag cggttgtaga acacgccgac gctgccgcgg ttcggcccgc tcgcatagcg  601 cagcgacagg tcggtcgaca cggccttttc cttcgacgcg ttcgggttgc cgatcaggaa  661 ctggccggtc gcatcgtgcg ggccgttcga atacagctcg tagaaggtcg gcgcgcgttc  721 ggtgtaggcg acgttcgctg cgatcgacca taccggcgtc agcgagaaca gcgcaccggc  781 cgacaggctg cccgcattga agtcgcgcgg ctgcgcgccc gcgaatttct cgacgcccgc  841 cggatcggga tcgaccttca cgtgctcgaa gcggccgccg aggctcagct tcagcgccgg  901 gacgacctgc cattcctcga ggccgaacag cgcgacgctg ttggtgcggg tggacggcac  961 gagcatttcg tcgccgagcg ccgagaacgt gttctggccg aactgcacgc cgatcgcgcc  1021 ttcgaacggc ccgatcttgc gatggcgtgc ctcgatgcgc gcctcgtaac cgcgattgcg  1081 gaaggtggtc gccgtctcgc cgttgtccac ttccttgtgg cggtagtcgg tgtacgcgaa  1141 atcgaatttc agcttcgtga acggcccgct caggttgcgc acctcggacg cgaacgcgag  1201 gcgttcctgg cgcatccgca gccgcacgtc gctttccgcg acggagccgt agttcgattc  1261 gtagccgctg tacgacaggc ccgcgaaacc gtccgcccac gtgtacgacg cgccgaccgc  1321 accgccgtgc acgcgcccgt cgctgttcgg cacgttgcct tccggttgcg gcgcgtcggg  1381 gccgtcgatc gcgcgctgct ggctgctgcg cgcatagccg ggaatccgca gcttgctggt  1441 ttcgcgatcg aacgcgtcga cgtggaacgc gaagcggccg ttgccgcctt cgacctgcgc  1501 ggcgccggcg cgcacggaat tcgcgccgcc gtagcgggcg tcgagcgcgc ccgtcatgcc  1561 ttcgatcgct tcgcgcggaa tccggttgtc gatcgtgttg accacgccgc ccaccgcgtt  1621 gccgccgtac agcagcgcgg ccgggccgcg cacgatctcg acgcgctcga tcgacagcgg  1681 gtcctgcggc accgcgtgat cgtacgacag cgacgacgcg tcatacgctg cgacgccgtt  1741 ctgcagcagc cggatccggt cgccgtccat cccgcggatg atcgggcggc cgaccatcgg  1801 cccgtaggtg gtggtcgaca cgccgggcag cccgttgagc gtttcgccga gcgaatcggc  1861 ctggcggcgc gtcagcgcgt cgccggaaag ctggacggtc ggtgcgatca gttcggtgtc  1921 gccgagcggg ttcgcggtca cgaagatcgg cgcgagcggt gcgggtgcgg cggcaggcgt  1981 gccggacgcg gcgggcgcat cggtctgcgc atgggcgacg gcggcgcaca gcatcagcga  2041 cagggacgag agcgggcgaa gcggtaagtg agggaggtcg cgcatggtcg gtgaatcggt  2101 tggaatcgga tgatcggata acgagcgatg cgggtcactg ttagatacga tatattgttg  2161 cgtaactgac gcgcagtgac ccgttggaac atggctcggc aaggcgcggc cacctggttg  2221 gaacggcat  163 1 mpfqpggral pshvptghca svtqqyivsn sdphrslsdh piptdsptmr dlphlplrpl  61 sslslmlcaa vahaqtdapa asgtpaaapa plapifvtan plgdteliap tvqlsgdalt  121 rrqadslget lnglpgvstt tygpmvgrpi irgmdgdrir llqngvaayd asslsydhav  181 pqdplsierv eivrgpaall yggnavggvv ntidnripre aiegmtgald aryggansvr  241 agaaqveggn grfafhvdaf dretsklrip gyarssqqra idgpdapqpe gnvpnsdgrv  301 hggavgasyt wadgfaglsy sgyesnygsv aesdvrlrmr qerlafasev rnlsgpftkl  361 kfdfaytdyr hkevdngeta ttfrnrgyea riearhrkig pfegaigvqf gqntfsalgd  421 emlvpstrtn svalfgleew qvvpalklsl ggrfehvkvd pdpagvekfa gaqprdfnag 481 slsagalfsl tpvwsiaanv ayteraptfy elysngphda tgqflignpn askekavstd  541 lslryasgpn rgsvgvfynr fsnylteynt grvvdddgep vapgtdgsln eaiyrgvrae  601 fygveldgkw rafsrrghtv dleltadyth arnvdtgqpl priaplratl aadygygpfg  661 araqvthaws qhrvpdddfs tdgytslgvm ltykfrvgpt hwlahlrgdn ltnqeiryst  721 svvrgfapqg grslmaglrt tf  725 43 107023546 YP_621873 188 1 ttattgcgcg ctcgacgcgg cggcagcact cgccggcgcg gcgcccgatg cggcggcacc  61 ggaagcggca gcggcgccgg acccggcagc ggccggcgcg gcgggggcag cggcacccga  121 ggcggcatcg gccgccgggg cgagctgcgc ggcgacggtc ttcacggtcg cgcccgggcg  181 caccttgtcg acgccttgca cgatcacgtg atcgcccgcc tgcagaccgc cttcgacgat  241 ccagttctgg ccttgcatgc cggtggtctt cagcggacgc ggctcgacct tgttgctggc  301 gttcaccacc atcgcgatcg cctggccctt ctggtcatgc gtgacgccga tctgcggcac  361 caggaaggcg ttctcgttca cgccttcctc gatccgtgcg cgcacgaaca tccccggcag  421 cagcacgcgg cccgggttcg ggaacaccgc gcggatcgtc accgagccgg tggtctggtc  481 gaccgtcacg tccgagaact gcagcttgcc cggctccgag taggtcttgc cgtcttccag  541 gatcagcgac accttcgccg cgcccgggcc gctcgtcttc agacggccgc tctgcacgtc  601 ctgacgcagc ttcagccctt cgaggctcga ctgcgtgagg tcgacgtaca ccggatcgag  661 ctgctgcacg gtcgacatca gcgtcgcctg gctcgcctgc acgtatgcgc ccggcgtcac  721 ttgcgagatg ccgacgcggc cgctgatcgg cgacacgacg tccgtatagc cgaggttgat  781 ctgcgcggta tcgaccgccg ccttgccggc cgcgacgtcc gccgcggcct ggccttgcgt  841 ggcgaccgcg ttgtcgtagt cctgcttgct gaccgcgttc gcggccacca gcaccttgta  901 gcgcgcgacc agcgcgttct gcgtgacgag gttcgcctgc gccttcgcga gcgtcgcctt  961 cgcgctgttc agcgccgcga tgtacggcgc cggatcgatc ttgtagaggc gctgaccggc  1021 cttgacgtcg gtgccttcgg tgaattcacg gcgcagcacg atgccgtcga cccgcgcacg  1081 cacttgcgcg acgaggaatg cactggtgcg gcccggcagg tcggtgaaga ccggtacggc  1141 ttgcggctgg acggtgacga cgccgacttc cggcgtttgc ggcggcggtg ccgattcttt  1201 tttcccgcac gcggccagga aaacggcggc cgtcgcgaca gtgattaagc ggtatggaac  1261 ccgttcgacg cgcat  164 1 mrvervpyrl itvataavfl aacgkkesap ppqtpevgvv tvqpqavpvf tdlpgrtsaf  61 lvaqvrarvd givlrrefte gtdvkagqrl ykidpapyia alnsakatla kaqanlvtqn  121 alvarykvlv aanavskqdy dnavatqgqa aadvaagkaa vdtaqinlgy tdvvspisgr  181 vgisqvtpga yvqasqatlm stvqqldpvy vdltqssleg lklrqdvqsg rlktsgpgaa  241 kvsliledgk tysepgklqf sdvtvdqttg svtiravfpn pgrvllpgmf vrarieegvn  301 enaflvpqig vthdqkgqai amvvnasnkv eprplkttgm qgqnwivegg lqagdhvivq  361 gvdkvrpgat vktvaaqlap aadaasgaaa paapaaagsg aaaasgaaas gaapasaaaa  421 ssaq  Bmu17616-800 ID MW Protein GI# Protein Locus# SEQ ID NO: Sequence 801 81 161524767 YP_001579779 212 1 tcaccagtta tatttcgcgg tggcgatcac cgtgcggtcg ttgccgaaca tgcagaccga  61 ggtcgactgg caaccgctga tgtaatgacg gttgaacagg ttcgtcgcgt tcaccgcgaa  121 gcgccagttg cgcacgtcgt aatgcacggc cgcgtcgatc agcgtgtagc tcgacaccgt  181 cagcgagttg tcggccgcgc cggcggacgc gctctggtag cgcacgccgc cgccgagccc  241 gaagcccgcg agcggccccg tgtgccacgt ccagtcggcc cacagcgacg ccatctggcg  301 cggccgcgga atgtcgaccg gccagttgtt cagcgtcgcg tcgttggcct gcacgacctt  361 cacgtcctga tacgcgtacg acgcgatcag cgacaggttc ggcgtgacct tgccggttgc  421 gctcagctcg atcccgcgcg cgcgcacttc accggtctgc acggatttcg tgccgcccgg  481 atcctgcgtc ggcagcgccg gcgtaacgcc gttggtctgg ttgatctgat agatcgcggc  541 attcagcatc aggttcttgc cgggcggctg ccagcgcagg cccgcttcga tctgcctgcc  601 gcgcgtcggc ttcggcaccc cgccgtcgag caggctcacg ccgatgatcg gattgaacga  661 cgtcgaatag ctgatgtacg gcgacagccc gtagccggcc tgatacgtga ggccgacgcg  721 gcccgtaaac gccgacacgt cggccttcga ctgcgtcccg gccgcgcggt cgtccatccg  781 catgttgacc cagtcctcgc ggccgccgag cgtcagcgtc cagcggttcc acttgatctg  841 gtcctgcgcg tacaggccga acgtgttcat cgtcgtgtac gtgttggtgc ggtacgtggt  901 gtccggatcg gaaaacacgg ccgtcgtgac cggcgtgtac accgggttgt agaggttcag  961 cgtcggcgcg gcggcgagcc attcgctatc ggtcgccgtc tggcggttgt actggaagcc  1021 gagcagcagt gtgtgctgga gcggacccgt cgcaaaacgg ccctcgaggt tgttgtcgat  1081 gtcgaagcgg ctgtagttca tctggaacac gccggcccag cggttgattt cggtcagcgt  1141 ttcgtcggcg aagcccgctc cccacaccga gccgttgtcg accgacagat gcatccatcg  1201 cgtgttctgc cggaacgtcc acatcgagtt caggttgtgc tcgaactgat agccgagcga  1261 ccactgcttc ttgcggtagt agttgaagtt gccgtcgcct tcgtagatgt ccttcgtgat  1321 ctgcccgttc ggattcggca gcaccgtgcc ggccgcgggc aggaagttcg acgagatgtc  1381 gccccagtcc tgcagatacg tggccgacag cgtcagcgac gtgtcggcgt tcgggcgcca  1441 gcggaacgac ggtgcgagcg cgacgcgctg atcgttgttc gggccggtca ccgcgttgcc  1501 gtcgcgcgcg acgccgacga accggtacgc gtacttgccg tccggatcga gcttgtcgcc  1561 gacgtcgatc atgaactgct tgcgtgcgta gttgccgatt tcgacgcccg cttcgcgcac  1621 gcgctcgccg tcggcgagct tcgtcttcac gtcgacgatc gcacccggat cgcccgcgcc  1681 gtacagcacc gacgtcgggc cgcgcagcac ggtgatgctg tcgatcatgt acggatcgac  1741 gcgccagctc gacaggttca gcgtgttggg cacctgcagg ccgttcacgt acgcggtcgg  1801 cgtgaagccg cgcagcgccg cgtaccagtc ggagcggttg tccgagccgt acgacgaaaa  1861 gcccggcacg tagcgcagcg cggtgttcac gtcggttgcg cccgtcatct cgatctgctg  1921 cgcggtcacg acgttgatcg tctgcgggat ctcctcgatc ggcgtgtcgg tcttcgtgcc  1981 ggtccggctg cgcttcgcga cgagccccac cgtgccgtcg ccttccgacg ccgcgttcac  2041 ggtgatcgcc ggcagcgtgc cggccgacgc agcgttcggt gcagcggcct gcgcattcgc  2101 cgccggcgtg gtctgcgcga acgcctgtcc ggcagccgcc gcaccgaatg ccacgctcgc  2161 cgcggccgcg atcgcacgca accgcgtgcc tgttgcccaa tccat  189 1 mdwatgtrlr aiaaaasvaf gaaaagqafa qttpaanaqa aapnaasagt lpaitvnaas  61 egdgtvglva krsrtgtktd tpieeipqti nvvtaqqiem tgatdvntal ryvpgfssyg  121 sdnrsdwyaa lrgftptayv nglqvpntln lsswrvdpym idsitvlrgp tsvlygagdp  181 gaivdvktkl adgervreag veignyarkq fmidvgdkld pdgkyayrfv gvardgnavt  241 gpnndqrval apsfrwrpna dtsltlsaty lqdwgdissn flpaagtvlp npngqitkdi  301 yegdgnfnyy rkkqwslgyq fehnlnsmwt frqntrwmhl svdngsvwga gfadetltei  361 nrwagvfqmn ysrfdidnnl egrfatgplq htlllgfqyn rqtatdsewl aaaptlnlyn  421 pvytpvttav fsdpdttyrt ntyttmntfg lyaqdqikwn rwtltlggre dwvnmrmddr  481 aagtqskadv saftgrvglt yqagyglspy isystsfnpi igvslldggv pkptrgrqie  541 aglrwqppgk nlmlnaaiyq inqtngvtpa lptqdpggtk svqtgevrar gielsatgkv  601 tpnlsliasy ayqdvkvvqa ndatlnnwpv diprprqmas lwadwtwhtg plagfglggg  661 vryqsasaga adnsltvssy tlidaavhyd vrnwrfavna tnlfnrhyis gcqstsvcmf  721 gndrtviata kynw  802 55 161519924 YP_001583351 213 1 atgcttgagg acaacaagat ggacaacatg cacaacacaa acggcctgat gcgcatcgcg  61 aaggtggcgg ccgcgagcac gctgctcgcg acgctgctcg ccgcgtgcgc ggtgggcccc  121 gactacaagc gcccggacgt cacgacgccc gcggcgttca aggaagcgcc gacgctcgca  181 cccggcgagc aggccggtac ttggaagccg gccgagccgg ccgacggcgc gcatcgcggc  241 gaatggtgga aggtgttcgg ggatcccgtg ctcgacgcgc tcgaggaaca ggcgctcgcc  301 gcgaaccaga acctgaaggc cgcggccgca cgcgtcgagg aggcgcgcgc cgcgacgcgc  361 actgcgcgct cgcagtggtt cccgcaggtc ggcgtcggct tcgggccgac gcgcgaaggg  421 ctctcgtcgg cgtcgcagtt ccagccgcag ggcacagggc cgaccaatgc gacgctgtgg  481 cgcgcgcagg gcaccgtgtc atacgaggcc gacctgttcg gccgcgtgag ccgcaacgtc  541 gaagcgtcgc gcgccgatca ggcgcagagc gaagcgctgt tccgttcggt gcagctcgcg  601 ctgcaggccg acgtcgcgca gaactacttc gaactgcgcc agctcgattc ggatcaggac  661 ctgtaccgcc gcacggtgga gctgcgcgag gaagcgctga agctcgtgca gcgccggttc  721 aacgaaggcg acatcagcga gctcgacgtg tcgcgtgcga agaacgagct tgcgtccgcg  781 caggccgatg cggtcggcgt cgcgcgccgg cgcgcggcgt ccgaacatgc gctcgcgatt  841 ctgctcggta aggcgccggc cgatttcgcg ttcaaggaaa cgccgctcgt gccggtcgcg  901 gtgaagattc cgccgggatt gccgtccgcg ctgctcgaac gccgtccgga cgtcgcggcg  961 gccgagcgtg cgatggcagc tgcgaacgca cggatcgggc tcgcgaagtc cgcgtatttc  1021 ccgaagctcg acatcaccgg atcgttcggc tatgaagcgt cgacgctcgg caacctgttc  1081 ctgtggtcga gccgcacgtt cctgctcgga ccgttcgcgg gcaccgcgtt gacgctgccg  1141 ctgttcgacg gcggacgccg cgcagccggc gtgcagcagg cgcgcgcgca gtacgacgaa  1201 caggtcgcga actatcggca gcaggtgctc gtcgcgttcc gcgaggtcga ggacaatctc  1261 gccgatctgc ggctgctcga cgatcagatt cgcgcgcaga atgcggccgt caatgcgtcg  1321 cgccgtgcgg cgacgctgtc gcggacgcag tatcaggaag gcgaagtcgc gtatctcgac  1381 gtgatcgata gtgagcggtc cgtgttgcag tcgcagttgc aggcgaatca gctcacgggt  1441 gcgcaggccg tgtcgaccgt caacctcatc cgtgcacttg gtggcgggtg gggtgaggcg  1501 ccgacggcgg tcgacggggc ggcggcggcc aaggcggaga tcgcggggcg ttga  190 1 mlednkmdnm hntnglmria kvaaastlla tllaacavgp dykrpdvttp aafkeaptla  61 pgeqagtwkp aepadgahrg ewwkvfgdpv ldaleeqala anqnlkaaaa rveearaatr  121 tarsqwfpqv gvgfgptreg lssasqfqpq gtgptnatlw raqgtvsyea dlfgrvsrnv  181 easradqaqs ealfrsvqla lqadvaqnyf elrqldsdqd lyrrtvelre ealklvqrrf  241 negdiseldv sraknelasa qadavgvarr raasehalai llgkapadfa fketplvpva  301 vkippglpsa llerrpdvaa aeramaaana riglaksayf pklditgsfg yeastlgnlf  361 lwssrtfllg pfagtaltlp lfdggrraag vqqaraqyde qvanyrqqvl vafrevednl  421 adlrllddqi raqnaavnas rraatlsrtq yqegevayld vidsersvlq sqlqanqltg  481 aqaystvnli ralgggwgea ptavdgaaaa kaeiagr  803 84 161520344 YP_001583771 214 1 tcagtagcgc gcgttcagcg tgacgaacgc ggagcggccc ggcgcgatcg acgcgtagtg  61 cgccggatac gcctgatcga agtacgtgcg gttgaacagg ttgttcacgt tcagctgcag  121 gtcgagcttc ttgttgatgc ggtactgcgc catcgcatcg aagcgccagt acgacggcac  181 cgcgcgcagg ttcgccggat cgccgaacac ttccgacatg tagaacgcgc cgccgccgac  241 cgtgaacttc ggcgtgacgt cgtagttcgt ccacatcgtg aagctgtgct tcggcgtatt  301 cgggaagcga tggccgttgt tcgcggtgtc cttgccgttg tcgcgcagtt cgctcttcat  361 gtacgtgtag ccgccgaaca cctgccactg tttcgtgagc tggcccgcga cgccgagctc  421 gaggccctgc acgcgcttgt tgccgaccat cgcgtactga ttgttcggca gcgtcacgcg  481 cgcgttcgtc gtatcgatct ggaacagcgc ggccgtcagc gacagcttgt cgttcagcac  541 gttccatttc gtgccgacct cgatgctgcg gttcttctcg ggcgacagct ggtcggcgtt  601 cgagccgacg ccgccgcggc ccggcgtcag cgactgcgtc tcgctgcctt cgccgagcag  661 catcccggcc ggcgtcgacg acgtcgcgta cgacgcatag atgctgccgt tctgcgcggg  721 cttgaacacc gcgccgagct gccagttgaa cagcgtgtcg tcgcgcgtgt aggtcttgcc  781 gccgttcgcc ttggtatcgg tgaagcgcgt cgagtaatcg tcgacgcgca cgccggcatt  841 gagctgccag cgcggcgtca gctcgatcgt gtcgaagccg tagatcgact tcgtcgtcgt  901 gcgcgcatgc gcatagtcgt tgttgcgcga gatcgaaccg gcccacggat cggccgggtt  961 cggcgaccac aggctcgtgc agttgtagcc ggacggcgcg ccgatgccgt tgttgcagat  1021 cgcgccggtg ccggtcgcga ccgtgtacgt gtcgcgcttg ccccattcgc gcgacagctc  1081 gatgccggtc gtgaagctgt gcttgaacgg accggtgcgg aattcgccga acagctcggt  1141 ctggttcgcg atgctgttga tcgaactgtt gcggttgttg ttgcggcgcc agaccttgcc  1201 gttgatcacg ttgccctgac tgtcgtcggg ctgcgtccag atgtagtcct gcgtcgattc  1261 ggtgtagcgc gtcgtgttgc gcacggtcag cgccggcgtg atgtcgtgct cgatgcgcag  1321 cgtgctgatg tccgacgtcg tcttgcggaa gtcgcggtcg atcaggccgt agaagttgtg  1381 gcggtcgacg ggcgccggat agatcgtgtc gacgttcgcg ggcttgttcg tcgtcgtgta  1441 gaagtacgga atgccgccgt cgggcaggtc gtccgtcgac agatggtagt agctcgcggt  1501 cacgcgtgtc ggcgtgccga ggccgaacgc gatcgacggc gcgacgcccc agcgttcgtt  1561 gttcacggcg tcgcggcccg ccacgtcgtt gttgtggctc atcaggttca gccggaacgc  1621 cgcatgatcg gcgaactgcc agttgccgtc ggcggtgaag cgacggtagc gatcggtgcc  1681 gagcccggcg ctcgcggcgg ccgtcgtgcc gaggtgcggc gccttcgtga tcaggttgat  1741 gctgccgccc gcgccgccgc ggccgccgta cgcgccgtcc gagcctttcg tgatctcgac  1801 gcgttcggtg ttgaagattt cgcgcgtggt cgcgccggtg tcgcgcatgc cgtcgacgaa  1861 catgctgccc tgcgtgtcgt agccgcggat gaacgggcgg tcgccgagcg ggttgccgcc  1921 ttcgccggcg ccgaacgtga tgccgggcac ggtgcgcagc gcttcggtca gcgtcgaggc  1981 gccgctcgtc tggatcagct cctgcggaat cacggtgacc gatttcggcg tgtcgacgag  2041 cggcgcggtg aatttggccg acgcggaaaa atcggccttg tagctgtgct cagcgcggcc  2101 cttgatctcg atcggcgcga gatggccttc cgcgtctgcc ggcgcgggcg gcggggcgcc  2161 gtcggcggcg aatgcgggac cggcggcgag cacgctgcac agggtggtga attttccgag  2221 cttcgactcg tcggaacggg acttcat  191 1 mksrsdeskl gkfttlcsvl aagpafaadg apppapadae ghlapieikg raehsykadf  61 sasakftapl vdtpksvtvi pqeliqtsga stltealrtv pgitfgageg gnplgdrpfi  121 rgydtqgsmf vdgmrdtgat treifnterv eitkgsdgay ggrggaggsi nlitkaphlg  181 ttaaasaglg tdryrrftad gnwqfadhaa frlnlmshnn dvagrdavnn erwgvapsia  241 fglgtptrvt asyyhlstdd lpdggipyfy tttnkpanvd tiypapvdrh nfyglidrdf  301 rkttsdistl riehditpal tvrnttryte stqdyiwtqp ddsqgnving kvwrrnnnrn  361 ssinsianqt elfgefrtgp fkhsfttgie lsrewgkrdt ytvatgtgai cnngigapsg  421 ynctslwspn padpwagsis rnndyahart ttksiygfdt ieltprwqln agvrvddyst  481 rftdtkangg ktytrddtlf nwqlgavfkp aqngsiyasy atsstpagml lgegsetqsl  541 tpgrggvgsn adqlspeknr sievgtkwnv lndklsltaa lfqidttnar vtlpnnqyam  601 vgnkrvqgle lgvagqltkq wqvfggytym kselrdngkd tannghrfpn tpkhsftmwt  661 nydvtpkftv gggafymsev fgdpanlrav psywrfdama qyrinkkldl qlnvnnlfnr  721 tyfdqaypah yasiapgrsa fvtlnary  804 83 161519887 YP_001583314 215 1 tcagaaatcg accttcatgc tgactgcgac ggtacggccg ggcgacgtat aggcgtcgag  61 caccggcgag tcggcggcga tgccgcgcac gtcggaccag ttccagtact tgcggtcgaa  121 caggttgcgg atgccgaccg tcgcgctcac gtgcttgttg aagcgatagc cgccgcgcag  181 atcgacgaca aacgacgaag gcggcgcgaa gcaggctcgc ttcgaacagt cggaccggtc  241 gatgtccttg tcgcgcttcg cggcctggaa cagcagatcg gtctgcacga accagcgttc  301 ggtcggctcg tagcgcacgc cgaacacggc agaaaacgga ttcacggtgt tgagcggctg  361 gctcgcggcg ccgtcgtttt gcgtcgagcc gcgcgtcagc gcgagcgcgg tgcgcagcgt  421 gatgccgttc ggcatcgccc agtcggcgcg gccttcgatg ccgtgaacgc gcgcatcggc  481 gaagttcacg tactggaaca cgaacggatc ggtcggccgg ccgctgccgg cgatcgtcgt  541 acgcgcgatg aagttgcggt agcggccggt gaacgcggcc gcgctgtagc gaatcgtgcc  601 gtagccggtg ccggcccgcc cgcgcagccc ggcttcgaac gtgtcgctgg tctcgggctt  661 caggttcgga ttgccgatcg aggtatagcc ctgcacggga ttcgagaagc tgctgttgac  721 ctgatcgggc gtcggcgcgc ggaatccatg tgcgtactgc agatacggaa tcagcgcagg  781 cgcgatctcg tacagcaccg cgacgcgcgg cgacagttcg ttcgcgtgcg tcgacaccgc  841 gcggccggtg aacagcggat cgttgcccga cggcttcagc cggtacgtgt cgaagcgcag  901 ccccggcgtg acgagcaggc ggccgtagcc gatctgatcc tgcacgaacg cgccgaacag  961 cgtgtagtcg gtgtcgggga aggccttgtt cgggaacgtc tcgccgacgc cgggcaccgt  1021 gccgtcgcgc aggttcgtga tccgcgacag gctgccgtcg acgccgccca gcagcttgtg  1081 cgcaagcggg ccggtcgaga atccgctttc ggcgaacgcg gagccgccga acgtgcgttc  1141 gcggtagcgg ttgtcgcgcg agcgtgacgg cagcgtgccg cgcgtttcga acgcgtactg  1201 gtcctgcgtc gcttcctggt aatagaactg cacgtgcgcg ttctgcagcc agcgcgaggc  1261 gtcgtcgtga aaggcgtaat cggcgctgaa gcggttgcgt tcgagccggt cgctcgtcgt  1321 gaggccgagc gtcgcgggcg cgacgatcgc agatagcacg tcggtgccga cgcgccgctg  1381 cacggtttcg gcggtcagcc tgatcgtgtc gcgcacggtg ggcgtcagca cgagcttgcc  1441 gagcagcgat tcgctgtaca cgtgctgcgg gttcgacgtc gtgcgcagct tgcctgcgga  1501 gttgttgctg ccgcgcgtgt cgatctcgtg accgcggcgg ccgtcggcga tgatcatccc  1561 ctggatgcga tcgttgccgc cggcggccga cacggtcgcg ccgatgctgc ggtcggtcga  1621 atcgtagctc ggccggaacg agaagtaggt cggcttgtgg tagatcgaca gcaggtcctg  1681 cggatccttc gtgatgaagt tgacggcacc ggtcagtccg tcgctgccgt acagcgccga  1741 cgccggcccg cgcacgatct cgatgcgctt cagcgtatcg agatcggtgt agtcgccgcg  1801 gcccgcctcg agcgggccga acgagaatgc gttcggcagg cggatgccgt cttccatcag  1861 catcacgcga ttgccttcga ggccgcggat gttgatgctc gaatcgccgt cgcggccgcc  1921 gccgagcgcc gcgttgccgg gacggtacgc ggtgcgccgc accgtgatgc cgggctcgta  1981 gcgcaaggcg tccttgatgt tggtggcctg ctgttcgtcg aggtcgtccg cgtcgatcac  2041 cgacacggaa gcggcggtgc ggctcgcggc cgtcgcggtg cgcgtcgccg tgacggtgac  2101 cgggtcgagc agcgcgctgc tggcgcgtgc ggctgccgcg tcggccgtga cggacgaggc  2161 ggcggtgtgg gacggcgaag aatcggcgtg agccgtggcg gcggacaatc cgaccgcgcc  2221 gatgagcgcg gcaaagatcg gctggcgcgc cagcgtagag cgatgcac  192 1 mhrstlarqp ifaaligavg lsaatahads spshtaassv tadaaaaras salldpvtvt  61 atrtataasr taasvsvida ddldeqqatn ikdalryepg itvrrtayrp gnaalgggrd  121 gdssinirgl egnrvmlmed girlpnafsf gpleagrgdy tdldtlkrie ivrgpasaly  181 gsdgltgavn fitkdpqdll siyhkptyfs frpsydstdr sigatvsaag gndriqgmii  241 adgrrgheid trgsnnsagk lrttsnpqhv ysesllgklv ltptvrdtir ltaetvqrrv  301 gtdvlsaiva patlglttsd rlernrfsad yafhddasrw lgnahvgfyy qeatqdqyaf  361 etrgtlpsrs rdnryrertf ggsafaesgf stgplahkll ggvdgslsri tnlrdgtvpg  421 vgetfpnkaf pdtdytlfga fvgdqigygr llvtpglrfd tyrlkpsgnd plftgravst  481 hanelsprva vlyeiapali pylqyahgfr aptpdqvnss fsnpvqgyts ignpnlkpet  541 sdtfeaglrg ragtgygtir ysaaaftgry rnfiarttia gsgrptdpfv fqyvnfadar  601 vhgiegradw ampngitlrt alaltrgstq ndgaasqpln tvnpfsavfg vryepterwf  661 vqtdllfqaa krdkdidrsd cskracfapp ssfvvdlrgg yrfnkhvsat vgirnlfdrk  721 ywnwsdvrgi aadspvlday tspgrtvavs mkvdf  807 81 161521211 YP_001584638 216 1 ctacagcttc gcgctgacgc gcacccaggc ggtgcggccc ggctcgttga ccggcgcgtt  61 cgccggatag ccgaagcccg cattgccggc gaggttcaga tgctcggcat aggccttgtt  121 cagcaggttg tcgacgccga ccgagatctg cacggtcttg ctcacgttgt actgcgcatg  181 cagcgacagc acgccgaagc ccgcgctcgg gccgaagtcc ttgccgacca cgttgccctc  241 gttcaccgca tagcgatgct gcggcgcaac gacccgccac aaaccgccgg ccgaccacgc  301 gccgcgcgtg tactcgagcc cgacgcgcgc ctcgagcggc ggcatctgcg gcaacggatc  361 gccgctcgtc gcattgcgcc cccacgcgta cgcaaccgag gtctcgatac gcagcggtgc  421 gaccggccgc cagccgacgc cggcttcgcc gcccatgatc tgcgcgttca cgttgctcgc  481 ccgcgtggtc gggcccatca tgccgctcgc gtagtcgaac aggatgaagt cctgcacata  541 gcctgcatac gcggagaccc acgcgtcgag ccgatcgctc ttgtactgcg cgccgatgtc  601 gagctgcgtc gtcttctcgg gctgcacggc cgagaaagcg ttgaccgaac cggccggccc  661 gcgtttcgcg gaaaacagct cccagtagtc cggatagcgc tccgcatggc cgatgcccgc  721 ataccaggtc acgggcagcg acgccaggtc gcgctcgtag cgcaggaagc cgctcggcag  781 cacgcgcgcg cgatcgtcgt cgaacgtcgg gttcggccgg ctcgtcatca tgccgcccgt  841 cgtcgcgcgc ttgtcacgcg cgctcgcata gtcgacgcgc gcgccgccga tcacgcgcgc  901 cgcctccgcc gcgtaccacg tgagctcgcc gaacgcgccg gcgttccaca tcgtcgcctg  961 cgcgtcccac ggctggtcgc catagttctg ccgccccatc gcggcgcgcg aatcgagccg  1021 gttcgattgc gcgtcgacgc ccgtcacgag cttgaacgcg tcgccgaatc gccacgtcgc  1081 ggccgcacgc gcgccgagcg ttcgacgccg cacctcggac gccatccgca tcggcatgct  1141 gctcgccgga tcgggctgcc gcagcgtgta gttgtccatc acgtgatcgg cttcgttgta  1201 gtagacgcgc gcttcgatcc ggtcgagcac gtcgccgaga tgccgcttgt cgaacgacag  1261 gccgaacgtg tcgcggcgga agtgcgcgcc gtccatcccg cgccccgcat agcgcgcgta  1321 gccatcgccg gtgccggccg tcagctcgac gcgcgtgtgc acgtcgggcg tccagccgag  1381 cgcggcatcg gcgttccatt tgtcccattg cgacggcacc gtgcggccgt tgccgtcgcg  1441 gtagtcctgc gcatgcgcgt gattcgcgat gacgcggccg tagaaatcgg gcgtgccggc  1501 cgtcacgtcg acgttctggt cgttgcggcc gaacgacccg ccgacgacgc tgccgtcgaa  1561 gcgcatgccg ggccgctcga aacgcggcgt cgtgcgctcg aacagcacgg tgccggccga  1621 tgcgccgggg ccgtacagca ccgtctgcgg ccctttcacg accgtcacct tgtcgtagct  1681 ttccggtgcg atataggaag tcggcgcgtc catccggttc ggacacgcgc cgagcgtcgg  1741 catcccgttc gcgagcacgt tgagccgcga gccgaacatg ccgcgcagta cggggtcgcc  1801 gttcgttccg ccgctgcgga tcgacgtgaa gccggggatc gtcttcagat agtcggcgcc  1861 gtcgctcgcg ggcagcggct ggcgcggcgc cttcggatcg gtgacgacga cgagcggcgt  1921 cgacaacggc gacgcgacga cttcgacggg cagcaacagc atgcccggcg tgtcgggcgg  1981 tacatcggcg cgatgtgcgg gggcgtcggc gtcggccgcg gcggccgtcg cgctcagcac  2041 gccggcggtc agcgcgggaa cggcaagttt cagcatgcgc ggcgcgggcc gcgcgcaggc  2101 actgggcgac gccggcggcg tgcgcaacga aaaaatggtc at  193 1 mtifslrtpp aspsacarpa prmlklavpa ltagvlsata aaadadapah radvppdtpg  61 mlllpvevva splstplvvv tdpkaprqpl pasdgadylk tipgftsirs ggtngdpvlr  121 gmfgsrlnvl angmptlgac pnrmdaptsy iapesydkvt vvkgpqtvly gpgasagtvl  181 ferttprfer pgmrfdgsvv ggsfgrndqn vdvtagtpdf ygrvianhah aqdyrdgngr  241 tvpsqwdkwn adaalgwtpd vhtrveltag tgdgyaryag rgmdgahfrr dtfglsfdkr  301 hlgdvldrie arvyyneadh vmdnytlrqp dpassmpmrm asevrrrtlg araaatwrfg  361 dafklvtgvd aqsnrldsra amgrqnygdq pwdaqatmwn agafgeltwy aaeaarvigg  421 arvdyasard krattggmmt srpnptfddd rarvlpsgfl ryerdlaslp vtwyagigha  481 erypdywelf sakrgpagsv nafsavqpek ttqldigaqy ksdrldawvs ayagyvqdfi  541 lfdyasgmmg pttrasnvna qimggeagvg wrpvaplrie tsvayawgrn atsgdplpqm  601 pplearvgle ytrgawsagg lwrvvapqhr yavnegnvvg kdfgpsagfg vlslhaqynv  661 sktvqisvgv dnllnkayae hlnlagnagf gypanapvne pgrtawvrvs akl  808 81 161525105 YP_001580117 217 1 tcagaactgc agcgtggtca gcagcgacac ctgccgcgcg tcgccgatcg cgacgaagta  61 gcggttcgcg ctcgacgggt agtacgtgcg gttgaacagg ttcttcacgt tcagctggaa  121 cgacagcttc tgcttgccga gccgcgtgtc gtaggttgcg aacgcgtcgg cgagcacgta  181 cgacggcagc gtgaagctgt tcgccgaatc gccgggccgt gcgccgacat agcgtgcagc  241 cgcaccgatg cgcagatcgt cgccgcccgc gagcgtgccc acgtcgtaca cggccgcgag  301 cgacgcggtg tgacgcgcga cgttccacag ccggttgccg gcgtacagcg gatcctcgat  361 cgtcttcgcg tcgatgtacg cgtagctcgc gatcacgttc acgcgttcgc cgatgcggcc  421 cgacacgtcg agctcgacgc cgcgcgagcg cgccttgccc gacgtgcgcc agtcggtcag  481 cttcgtcgcg tcgttgaact gcgagacgag cacgttcttc ttgtcgatgt cgaacagcgc  541 gagcgtgccc gtgatgccgc ccggcaggtc gagcttgccg ccgacctccc acgcggtcgc  601 ctgctccggc ggcgtcgcgg cgtcgatcac gtagccggac gccatcggcg cgatcgacga  661 cgacggcttc agcgactgcg aatagctgcc gtacagcgag aagctatcgg tccacttgta  721 gacgatgccc gcgcgcggca gccagcgcga gccgctcaga tcggtgttcg catgaaacgg  781 ccggccgcgc cccgcgacct ggttgtacgt gatatagcgc acgccgcccg agacgatcca  841 cttgtcggtc agatgaatcg tgtcctgcag aaacgcggac gcgtcgtgca gcgcgtcggt  901 ctggtcgctg tcgctcgccg acaccgtgct cgacggcggc agcagcccgt acacgggatc  961 gacgtagctg aacggcgtct tcaccgcctg ccgcagcatg tccttgcgat agatgcggcg  1021 gtactcggtg tcgaagccga cctgcacgtc gtgccgcatc cccgcgagcg tcagcttgcc  1081 gttcacgtag ccgatcccgt agctgtcggt gctcagcgag ccgtgcgtcg cgtcgttgct  1141 gcgcgacagc gtacccgtca ccggatcgac gcccgtcgtg cgcagctggt tcgcatcgta  1201 ggtttcgcgg ttgtagctgt agccgaaatg cgcgctccag tccgcgttga actggtgatc  1261 gacgctcagc tgggcgagat gcgattcgcc gtccatgttg ttgaacggct cgtcgatgcg  1321 ccgccgcgcg ggaatgtcga gcggcgcgtt ggtgcgcgga tcgagcgccg tgccgcgatc  1381 gaacggcgag tggaacttgc ggtactcgta cgacaccacg acctgcgtgt cgcggccgta  1441 ccacgcgagc gacggcgcga cgaaggtctg ccggtattcg ccgaaattgc gccagtactg  1501 ctcgttcgac tggtcgacga tcagccggta cgcgagccgc gaatcgccga tcggcccggt  1561 cgaatcgaac gtcgcgctgc cgccgttctt gccgtgcccg aacgtcgatg cgccgagcga  1621 aaccgcgttg tagcgcgtga gctgcggctg cttgctgacg acgttgacga cgccgcccgg  1681 gtccatcagc ccgtacagca gcgaggtcgg ccccttcagc acctcgacgc tgtcgacggc  1741 ggcgttgaac gagcgcccct gcacgatcgg catgccgttg tgcatgaccg agccgtcgcg  1801 gttgtcgccg aagccgcgct tcatgatcgt gtcctgcgtg cccgcgagcg tgttgccctg  1861 cgtgatgccg ctgacgttgc cgagcgcgtc gtcgaggttg cgcggacgct ggtcgcgcag  1921 cacctgcgcc ggcacgacgt tgacggcctg cgcggtatcg agcaccggga tatcggcgcg  1981 cagcacgccc gcctccttcg gcggccggta gctttgcgcg cgcagcgcgc tgctgcgcac  2041 cgcgatcgtc ggcagttcgg ccgccgtatc ggccgacggc gccggcacgg cggcaccgct  2101 catgccgttc gcgccgttcg cgccgttcgc gcgcatcaac gtatagccgc cgcccgactg  2161 acgcagcgcg acgaggccag tgcccgcgag cacgcgcgcg agcgcatcgt cgacgtcgaa  2221 gcggccgtgc acgccgccgc tcgacaggcc ggcggtcagc tcaggcggaa acgcgatcag  2281 caggccggcg tcgcggccga accggttcag cgtcgcttcg agcggcccgg ccggcacgtc  2341 gaatgcgcgg cgcgacgtgc gcggcgcggc ggcggccgcg tcggtgtcgg catcggcgag  2401 cgcgggcaac ggcagcagcg cggacagcag gacggcgccg gcaagccggt gcgcgacgcg  2461 gttcggcgcg gcgggcacga tcgtgcgccg cgtgtggcgg gcggacgccg gacggcgccg  2521 cggcatgaga cggatggaaa ccatgatcga gaagtcggta aacgatgggc cggtgcggct  2581 ttcatttccc at  194 1 mgnesrtgps ftdfsimvsi rlmprrrpas arhtrrtivp aapnrvahrl agavllsall  61 plpaladadt daaaaaprts rrafdvpagp leatlnrfgr daglliafpp eltaglssgg  121 vhgrfdvdda larvlagtgl valrqsgggy tlmrangang angmsgaavp apsadtaael  181 ptiavrssal raqsyrppke agvlradipv ldtaqavnvv paqvlrdqrp rnlddalgnv  241 sgitqgntla gtqdtimkrg fgdnrdgsvm hngmpivqgr sfnaavdsve vlkgptslly  301 glmdpggvvn vvskqpqltr ynayslgast fghgknggsa tfdstgpigd srlayrlivd  361 qsneqywrnf geyrqtfvap slawygrdtq vvvsyeyrkf hspfdrgtal dprtnapldi  421 parrridepf nnmdgeshla qlsvdhqfna dwsahfgysy nretydanql rttgvdpvtg  481 tlsrsndath gslstdsygi gyvngkltla gmrhdvqvgf dteyrriyrk dmlrqavktp  541 fsyvdpvygl lppsstvsas dsdqtdalhd asaflqdtih ltdkwivsgg vryitynqva  601 grgrpfhant dlsgsrwlpr agivykwtds fslygsysqs lkpsssiapm asgyvidaat  661 ppeqatawev ggkldlpggi tgtlalfdid kknvlvsqfn datkltdwrt sgkarsrgve  721 ldvsgriger vnviasyayi daktiedply agnrlwnvar htaslaavyd vgtlaggddl  781 rigaaaryvg arpgdsansf tlpsyvlada fatydtrlgk qklsfqlnvk nlfnrtyyps  841 sanryfvaig darqvslltt lqf  809 27 161524037 YP_001579049 218 1 ttactggtgc tgcggcgcgc gcagatagat ctcgacgcgg cggttctgtg cgcggccggc  61 ttcggtcgag ttgtccgcga ccgggttcga cgggcccatg ccctgcgccg acaggcggtt  121 gcccgcgacg ccgcgctgca ccagcgcgtt gacgacgctt tgcgcgcggt tctgcgacag  181 cgtctggttc agctgcgcgg agcccgtgct gtcggtgtag ccgatcaccg acgccgtcac  241 ttgcgggttc tggttcagcg tcgttgccag gtcgttcagc agcggcgtga aggccggcgt  301 gatcgcgtac tggttcgtcg cgaacgtgac cgagctcggc acgttcagct tcagcgagcc  361 gtccggctgc tcggtgacct gcgtgcccgt ctgcgcagcc gacggcgcga gcttgttctt  421 gatcgcttgc cagttgtagc ccgtcacgcc gccgaccagc gcgccgacgc ccgcgccgat  481 cgccgcgccc ttgccgccgc ctgccagtgc gccgatgcct gcgcccagcg ccgcacccgt  541 gccggtgccg acggccgtgt tgttgccttg ctgcgtcgcg cagcctgcca gcagggcgcc  601 ggccagtgcg aagacggaca agcgggtcgc gattttcatg ttcat  195 1 mnmkiatrls vfalagalla gcatqqgnnt avgtgtgaal gagigalagg gkgaaigagv  61 galvggvtgy nwqaiknkla psaaqtgtqv teqpdgslkl nvpssvtfat nqyaitpaft  121 pllndlattl nqnpqvtasv igytdstgsa qlnqtlsqnr aqsvvnalvq rgvagnrlsa  181 qgmgpsnpva dnsteagraq nrrveiylra pqhq  810 19 161525757 YP_001580769 219 1 atgaccacaa ggagagtaac catgatgtcg aaaaaagttc gtctggccct ggccgtgatg  61 atgatcggcg cgctcgcagc gtgtaagtcg ggtgtgaagc tcgacgacaa ggcaaacgcc  121 ggtgcgatga gcacgcaacc gagcgccgac aacgtcgcgc aagtgaacgt cgatccgctg  181 aacgatccga acagcccgct cgcgaagcgc agcatctact tcgacttcga cagctattcg  241 gtgaaggacg agtatcagcc gctgctgcaa cagcacgctc agtatctgaa gagccacccg  301 cagcgccacg tgctgatcca gggcaatacc gacgaacgcg gcacgagcga gtacaacctc  361 gcgctgggcc agaagcgtgc ggaagccgtg cgccgcgcgc tcgcgctgct cggcgttgcc  421 gattcgcaga tggaagccgt gagcctcggc aaggaaaagc cgcaagcaac gggtcacgac  481 gaagcgtcgt gggcgcagaa ccgccgcgcc gatctcgtct accaacagta a  196 1 mttrrvtmms kkvrlalavm migalaacks gvklddkana gamstqpsad nvaqvnvdpl  61 ndpnsplakr siyfdfdsys vkdeyqpilq qbagylkshp qrhvliqgnt dergtseynl  121 algqkraeav rralallgva dsqmeayslg kekpqatgbd easwaqnrra dlvyqq  811 24 161525435 YP_001580447 220 1 atgaataaac tttcaaagct cgcgttcatt gcagctaccg cagttatggc tgcatccgct  61 tcggcacagt cggtgccggc gtcgcgtcaa gccgtcaatg acaactgggt gaacggcacg  121 ggcgaatggg tgtggatgaa cggcacgaac gagctctgct ggcgtgacgc gttctggacg  181 ccggccaccg ccaacgcgaa gtgcgacggc gcactggtcg cccaggcacc gcagccgccg  241 gtcgctccgg tcgctccggc catcacgagc cagaagatca cgtatcaagc cgacgcactg  301 ttcgacttcg acaaggcgac gctcaagccg ctgggcaagc agaagctcga cgaactggct  361 tcgaagatcc agggcatgaa cacggaagtg gtcgtcgcaa cgggctacac cgaccgcatc  421 ggttcggaca agtacaacga ccgtctgtcg ctgcgccgtg cgcaagccgt caagtcgtac  481 ctggtcagca agggtgtccc ggccaacaag atctacacgg aaggcaaggg caagcgcaac  541 ccggtcacga cgggctgcaa ccagaagaac cgcaagcaac tgatcgcctg cctcgcaccg  601 gatcgccgcg tggaagtgga agtggtcggt acgcaagaag tgcagaagac gaccgttccg  661 gcgcagtaa  197 1 mnklsklafi aatavmaasa saqsvpasrq avndnwvngt gewvwmngtn elcwrdafwt  61 patanakcdg alvaqapqpp vapvapaits qkityqadal fdfdkatlkp lgkqkldela  121 skiqgmntev vvatgytdri gsdkyndrls lrraqavksy lvskgvpank iytegkgkrn  181 pvttgcnqkn rkqliaclap drrvevevvg tqevqkttvp aq  812 40 161525565 YP_001580577 221 1 atgaaaaaga ccctgatctt cgccgccctg tccggcgcgt gcgcggccgc gcacgcgcaa  61 agcagcgtca cgctctacgg gctgatcgac gccggcatca cgtatacgaa caaccagggc  121 ggccacagcg cgtggcagga gacgagcggc tcgatcaacg gcagccgctg ggggctgcgc  181 ggtaccgagg acctcggcgg cggactgaag gcgatcttca cgctcgagaa cggcttcggg  241 atcaacaacg gcacgctgaa gcagaacggc cgcgagttcg gccgccaggc attcgtcggc  301 ctcgcgcaca gcgggttcgg ctcgctgacg ctcggtcgcc agtacgacag cgtcgtcgac  361 ttcctcggcc cgctgtcgct gacggggacg caatacggcg gcacgcagtt cgcgcatccg  421 ttcgacaacg acaacctgaa caactcgttc cggatcagca atgcggtcaa gtatcaaagc  481 gcagacttcg gtggactgaa gttcggcgca ctctacggct tctcgaattc gaccgatttc  541 tcgaacaacc gcgcgtacag cgtcggcgcg tcgtacagct tcatgggctt caacgtcgcg  601 gccgcctaca tgcaactgaa caacaacatc aattcgctcg cgctcgcggc cagcgatccg  661 ggcgcagtgg ccggcgactg gacgttcgcg gcaagccggc agcgcacttg gggcgccggc  721 ctcaactacg cgttcggccc ggcaaccgcg ggcttcgtgt tcacgcagac gcgcctcacc  781 gattcggccg gcatcagcgc cggccaatcg ggcgtgtcgg gcggcatccc gctgacgggc  841 gacacgcgct tcaacaacta cgaattgaac gcgcgctatg cactgacgcc ggcgttctcg  901 ctcgcgggct cgtacacgta caccgacggc cgcatggaag gacagaagcc gagttggcac  961 cagttcaacc tgcaggccga ctacgcgctg tcgaagcgca ccgatctgta tctgcagggc  1021 gaataccagc gcgtgaacgg ggacggtctc gcggtcggcg cgaacatcaa cggcctcggc  1081 gtcgcgtcgt cgaccaacaa gcagatcgcg gtgacggccg gcatgcgtca ccgcttctga  198 1 mkktlifaal sgacaaahaq ssvtlyglid agitytnnqg ghsawqetsg singsrwglr  61 gtedlggglk aiftlengfg inngtlkqng refgrqafvg lahsgfgslt lgrqydsvvd  121 flgplsltgt qyggtqfahp fdndnlnnsf risnavkyqs adfgglkfga lygfsnstdf  181 snnraysvga sysfmgfnva aaymqlnnni nslalaasdp gavagdwtfa asrqrtwgag  241 lnyafgpata gfvftqtrlt dsagisagqs gvsggipltg dtrfnnyeln aryaltpafs  301 lagsytytdg rmegqkpswh qfnlqadyal skrtdlylqg eyqryngdgl avganinglg  361 vasstnkqia vtagmrhrf  813 44 161520585 YP_001584012 222 1 atgaggaagc acgtcatttt tgccgccgcg ctcgccgcgt ttgccgcgcc ggccttcgcg  61 caggacagcg tgacgctgta cggtgtgatc gacgagggct tcaactacac gaacaacgtg  121 agcgtcaacg gcgcgggcaa ggccgattac cagctcgcga gcggctatgc gcagggcagc  181 cgctgggggc tcaaggggag cgaggatctc ggcggcgggc tgaaggcgat cttcacgctc  241 gaaagcggtt tcgacgtgaa caacggccgg ctcgggcagg gcagccggat gttcggccgg  301 caggcgttcg tcggcctcgg cacgtcgcgt ttcggcacgc tgacgttcgg ccgccagtac  361 gattcggtcg tcgactatct cgcgccgctg accgcgaacg gcaactgggg cggcacgctg  421 ttctcgcatc cgttcgacaa cgacaacacc gacaactcgt ttcgcgtgaa caacacggtg  481 aagtacgcga gcccggactg gaacgggctg tcggtcggcg gcacgtacag cttcagcaac  541 agcaccggct tctcgaacaa ccgtcagtac agcctcggcg cgcagtattc gctcgccggc  601 ctgcaggtcg cggccgcgta cctgcaggcc aaccatccgg gcgccggcgg cgcgggtgcg  661 atcgccgcgg acgacgcgaa cttcgtcgcc gagcggctgc gggttttcgg cggcgggctg  721 aactacacgt tcgggcccgc gacggtcggc ttcgtctaca cgaagaccga cttgaagaac  781 ccggtgtcga ccgtctatct gccggcgtcg acgttcgcgg gcctcgggct cggcgcgacg  841 aaattcgaca acttcgaggt caacggcaag tatcagctga cgtccgcgtt ctacgtcggc  901 gcgcagtacg tgtatacgga cggcaagttc gatgcggcga ccggatcgat caagccgaag  961 taccacacgg tcgggctgat ggccgactac agcctgtcga agcgcaccga cgtctacctg  1021 cagggcgcgt ggcaaaaggt ggccggcgat cgcacgggta ccgcggccga cggcggctac  1081 gtggtcggga ccgacggccc gtcgtcgtcg tcgaaccagt tcgcggtgcg cgccgcgatt  1141 cgtcacaagt tctga  199 1 mrkhvifaaa laafaapafa qdsvtlygvi degfnytnnv svngagkady qlasgyaqgs  61 rwglkgsedl ggglkaiftl esgfdvnngr lgqgsrmfgr qafvglgtsr fgtltfgrqy  121 dsvvdylapl tangnwggtl fshpfdndnt dnsfrvnntv kyaspdwngl svggtysfsn  181 stgfsnnrqy slgaqyslag lqvaaaylqa nhpgaggaga iaaddanfva erlrvfgggl  241 nytfgpatvg fvytktdlkn pvstvylpas tfaglglgat kfdnfevngk yqltsafyvg  301 aqyvytdgkf daatgsikpk yhtvglmady slskrtdvyl qgawqkvagd rtgtaadggy  361 vvgtdgpsss snqfavraai rhkf  814 39 161520486 YP_001583913 223 1 atgaacaaga ctctgatcgt tgcagcagct gcagcatcgt tcgcaaccgt cgctcacgcg  61 caaagcagcg tcacgctgta cggcgtgctg gacgcaggca tcacgtacca aagcaacgtt  121 cagccggccc cgggccaggc tggcaagtcg ctgtggtcga tgggctcggg cattgaccag  181 agccgcttcg gcctgcgtgg ttcggaagac ctcggtggtg gcctgaaggc gatcttcacg  241 ttggagagcg gcttcgacat cggcaacggc cgtttccgta acaacggcgg catgttcaac  301 cgtcaggctt tcgtcggtct gtcgagccag tacggcaccg tcacgctggg caagcagtat  361 gactccgttc aagactacct ggctccgctg accgcaacgg gcagctgggg tggtacgtac  421 ttcgcgcacg tcggcaactt cgacaacctg agcacgaacg gtggctactc gccgaacaac  481 agcatcaagt tcacgagcgc taactacgct ggcctgcaat tcggcggcac gtactcgttc  541 tcgaacaaca cgaacttcgg caacaaccgt gcttacagcg gcggcgtgtc gtaccagttc  601 caaggcctga agatcgcagg tgcgtactcg caactgaaca acccgggcca aacgaccggc  661 ggcgcagttg acacgctgca aacgcaaggc cgcgtccgta cgtacggcgc agctgctggc  721 tacgcattcg gcccggcaca agtcggcgca gcatggacgc aagctcgtct ggacaacacg  781 ggcgcgaccg gtacgtcggt tcgcatcgac aactacgaag tcaacggcaa gtacaacctg  841 acgccggctc tgggtctggg cgttgcttac acgtactcga acgcgcgtct gggtcaggac  901 agcgctcact ggcaccaagt tggcctgcag gctgactacg cactgtcgaa gcgtaccgac  961 gtgtacgcac aggctgtgta ccagcgtgct agcggcgcga acgcgtcgat ctacaacggc  1021 aacatcgaca cgctgccgag ctcgtcgatc aaccaaaccg cagcaacggt tggtctgcgt  1081 caccgcttct aa  200 1 mnktlivaaa aasfatvaha qssvtlygvl dagityqsnv qpapgqagks lwsmgsgidq  61 srfglrgsed lggglkaift lesgfdigng rfrnnggmfn rqafvglssq ygtvtlgkqy  121 dsvqdylapl tatgswggty fahvgnfdnl stnggyspnn sikftsanya glqfggtysf  181 snntnfgnnr aysggvsyqf qglkiagays qlnnpgqttg gavdtlqtqg rvrtygaaag  241 yafgpaqvga awtqarldnt gatgtsvrid nyevngkynl tpalglgvay tysnarlgqd  301 sahwhqvglq adyalskrtd vyaqavyqra sganasiyng nidtlpsssi nqtaatvglr  361 hrf  815 55 161521143 YP_001584570 224 1 atgaagaaac ttgctctctc ggccctctcg ctcgccctgc tgggcgccgc cggcgctgca  61 caggctcaat ccagcgtgac gctgtacggc gtgatcgata cgtcgattgc ctatgttcat  121 ggcaatgacg gccaagccaa caacatgtgg caaatgctgt cgggcaacct gcaaggcagc  181 cgctggggcc tgaagggtgc tgaagacctg ggcggcggcc tgaaggcaat cttccagatc  241 gaaaacggct tcaacccggg cacgggcaag ctgagcgcag caaacacgat cttcaaccgc  301 caggcattcg tcggtctgca aagcaaccag tacggtacgc tgacgctcgg ccgccagtac  361 gacccggtcg tcgaccttgt ccaggcagtc acggctgaca actacttcgg cagcttcttc  421 gcgaccccgg gtgacgtcga caacaacgac aacagcctgc gcgtcagcaa cgcgatcaag  481 tacacgtcgc ccgtctacgc cggcttccag ttcgaaggca tgtacggcct gagcggcatc  541 gcaggcaagc cgggccaggg tcagacgtgg tcggctgctg cggcctacaa caacggcccg  601 atcggcatcg cagccggcta cttctacgcg aacaacccgt cgccgacgac caccgcggtc  661 cgtggcggct ggggctcgac gacgtcggac aacatcgtcg acggcccgat caacgccggc  721 tatgtgacgg cgaagtcgat cggcatcgcg caagtcgcag ctcagtacgc gatcggcccg  781 gtgacgttcg gcctcggcta cagcaacgcg caatacaagc cggacgctta ctcgacgttc  841 tcgtcgaccg agaagtacaa cacgggccgc ggcttcgtga cgtaccaggt cacggcgccg  901 ctgctgctgg gcctcggcta ctcgtacacg aaggcaagcg gcaacacgga cgcgaagtac  961 caccaggttt cgctgggcgc agactactcg ctgtcgaagc gcacggacgt ctacctggtc  1021 ggcgcgtacc aacacgccag cggcacgcag ctcaacacgg acggcacgac gtcggcagcg  1081 caagcgtcga tcggctcgta tggctatgcc ggcacgaagt cgcaggagat ggttgccctc  1141 ggcctgcgcc acaagttcta a  201 1 mkklalsals lallgaagaa qaqssvtlyg vidtsiayvh gndgqannmw qmlsgnlqgs  61 rwglkgaedl ggglkaifqi engfnpgtgk lsaantifnr qafvglqsnq ygtltlgrqy  121 dpvvdlvqav tadnyfgsff atpgdvdnnd nslrvsnaik ytspvyagfq fegmyglsgi  181 agkpgqgqtw saaaaynngp igiaagyfya nnpsptttav rggwgsttsd nivdgpinag  241 yvtaksigia qvaaqyaigp vtfglgysna qykpdaystf sstekyntgr gfvtyqvtap  301 lllglgysyt kasgntdaky hqvslgadys lskrtdvylv gayqhasgtq lntdgttsaa  361 qasigsygya gtksqemval glrhkf  816 58 161524787 YP_001579799 225 1 tcacgcgtcg tcggcggccg tgccgggcgt ccagccaccg ccgagcgcgc gatacagcgc  61 gatcgcgttc gcgagccgca gctgcctgag ccggatcagc tcctgtcccg attcgtacgt  121 gctgcgctgc gcgtcgagca gttcgagata cgtcgcgacg ccgcccgcgt aacgacgttc  181 cgcgagcttc agccgcgcgt cgtcggccgc gtgcacgtcg cgctgcgcgg ccagctggcg  241 ctcgatccag tcgcgtgccg taaaggcgtc cgcgacctcg cggaacgcga cctgcacggt  301 cttctcatac tcggcgaccg caatgtgccg ccgcgcgttc gcgacgtcga gattcgcgcg  361 attgcgcccg cccgcaaaaa tcggcagcgt gatgcgcggc gcgaaggtcc agacgctcgt  421 gccggccgcg aacaggctcg aaaacgcgtc gctgaccgag ccgtaatcgg tcgtcagcgc  481 gatgcgcggg aaaaacgcgg cgcgcgcggc gccgatctgc gcgtgcgcgg ccttgagccg  541 cgactcggcc tgccggatgt ccggacgtcg ctcgagcaga tcgctcggcg caccgggcgc  601 gaccggcgcg atcgtcagcg cgtcgagcgc cgtatcgtcg tcgcgcacgt cgcgcgcgaa  661 atcgccggca agcagttgca gcgcgcgcac ggcctgcgcg tgctcgcgct gcagcgccgc  721 gtgcgatgca cgcgcggccg cgaccagcat ctcggccgaa cgcagttcga tcgcatcgct  781 cgtgccggcc gcatagcggc gttgcgtcag cgcggcgatc cgttcgcgcg catcgagcgt  841 gcgctcggcg agcgcgcgct gttcgttcag cgcgcgttcc gcgacgtagg cattcgcgac  901 ttcggcgatc acgccgatac ggaccgtgcg ctgcgcgtcg gccgtcgcga aatactcggc  961 cagcgctgca tcggacagat tgcgcacgcg cccgaacaga tcgagttcgt atgcgctgat  1021 gccgacgccc gcccgataca gcccgctcac cgcgctctcg cgcaggaccg gatcgtattg  1081 ccgcgtgcgt tcgtaaccgg tgctcgcgtc gatcgacggc atccgctccg cacgctgcac  1141 gccgtacatc gcgcgcgcct cgtcgagccg gccggccgcg atccgcagat cgcgattgtt  1201 ggcgagcgcc gcgtcgatcc atgcatgcag cgcgggcgcg gtgaaatagc tgcgccagtc  1261 gtcgagcagc gccgcatcgg cgcgcggcgc gaaagccgac ggcgcgtcgt gcgtggcgtc  1321 gacgccgttc gtatacgcgg acggcaccgg cgcagccgga cgctcgtagc gcggcgcgag  1381 cgaacagccg gccagcacga gcgcggccgc cagcgcgaac ggcgcgcgcc gcgcggtgcg  1441 gcgcgaatcg gaacgatgcc tcat  202 1 mrhrsdsrrt arrapfalaa alvlagcsla pryerpaapv psaytngvda thdapsafap  61 radaallddw rsyftapalh awidaalann rdlriaagrl dearamygvq raermpsida  121 stgyertrqy dpvlresavs glyragvgis ayeldlfgrv rnlsdaalae yfatadaqrt  181 vrigviaeva nayvaeraln eqralaertl dareriaalt qrryaagtsd aielrsaeml  241 vaaarashaa lqrehaqavr alqllagdfa rdvrdddtal daltiapvap gapsdllerr  301 pdirqaesrl kaahaqigaa raaffprial ttdygsysda fsslfaagts vwtfapritl  361 pifaggrnra nldvanarrh iavaeyektv qvafrevada ftardwierq laaqrdvhaa  421 ddarlklaer ryaggvatyl elldaqrsty esgqelirlr qlrlanaial yralgggwtp  481 gtaadda  817 55 161523870 YP_001578882 226 1 atgatgcaaa aacacgcttt gactgcaatc gcggtcgcgc tccttgccgc aggctgcacg  61 ttggcgccgc actacacgcg gcccgacgcg cccgtcgcgc aggcgtttcc gtccggcggc  121 gtctatgcga cgcagccggg cgctgccggc gcgcgcagcg cgaacggcca ggcggcgacc  181 gccatcggct ggcgcgaatt cttcgtcgat ccgcgcctgc agcggctgat cgagatcgcg  241 ctgaagaaca accgcgacct gcgcgtgtcg gtgctgaaca tcgaggcggc gcgcgcgcag  301 tatcagatca cgcgcgcggg cctgttcccg acgctcgacg gcacgggcac cggcaatatc  361 cagcgcgtgc cgcagggcgt gtcgcagacg ggtgcgccgt atatctcgcg cgtctacaac  421 gtcggcttgt cggcgtcgtg ggagctcgat ctgttcggcc gcgtgcagag cctgaaggac  481 caggcgctcg cgcagtatct gtcgaccgcg tatgcgcggc aggctgccga gatctcgctc  541 gtgtcgcagg tcgcggatca gtatctgacg ctgctgtcga ccgacgatct gctgaaggtc  601 acggaagaca cgctgaagac ggctcaggcc tcttacgacc tgacgaagct gcagttcgac  661 aacggcacgg gctccgagct cgagctgcgt caggcgcaga cggtcgtcga gcaggcgctc  721 gcgaaccagc aggcgcaggc gcgcgctcgt gcgcaggcgc tcaacgcgct ggtgctgctg  781 atcggcgagc cgctgccgga cgatctgccg ccggggctgc cgctcgatgc gcaaaatctg  841 ctcgccgacg tgccggccgg gctgccgtcg gatctgctga cgcgccgtcc cgacatcatg  901 caggccgagc agacgctgct cgccgcgaac gcgaacatcg gcgcggcgcg cgcggcattc  961 ttcccgcgca tctcgctgac gggcgcgttc ggcaccgcga gcccgacgct cggcgggctg  1021 ttcaaggccg gcaccgcggc gtggtcgttc gcgccgcaga tcacgatgcc gatcttcgaa  1081 ggcgggcaga acatcgcgaa cctgaacctc gcgaacgtgc agaagcgcat cgagatcgcg  1141 aactacgaga aggcgatcca gagcgcgttc cgcgaagtgg ccgacggtct ggccgcgcgc  1201 ggcacgtacg atcagcagat cgccgcgctc gagcgcaaca cgcatgcgca gcagcgccgc  1261 ttcgatctgt cggacctgcg ctacaagaac ggtgtcgaca gctatctgtc cgtgctgacc  1321 gcgcagaccg atctgtacac ggcgcagcag gcgctgatca acgcgcggct cgcgcgctgg  1381 acgaacctcg tcgatctgta ccgtgcgctc ggcggcggct ggatcgagcg ggccggcgag  1441 acgccgcggg cgcccgatca gacggtcgat tacgacaagg cctcggcgcc tgcgtcggcg  1501 tcggctgcgg cgacgaacgg gtaa  203 1 mmqkhaltai avallaagct laphytrpda pvaqafpsgg vyatqpgaag arsangqaat  61 aigwreffvd prlqrlieia lknnrdlrvs vinieaaraq yqitraglfp tldgtgtgni  121 qrvpqgvsqt gapyisrvyn vglsasweld lfgrvqslkd qalaqylsta yarqaaeisl  181 vsqvadqylt llstddllkv tedtlktaqa sydltklqfd ngtgselelr qaqtvveqal    241 anqqaqarar aqalnalvll igeplpddlp pglpldaqnl ladvpaglps dlltrrpdim 301 qaeqtllaan anigaaraaf fprisltgaf gtasptlggl fkagtaawsf apqitmpife  361 ggqnianlnl anvqkrieia nyekaiqsaf revadglaar gtydqqiaal ernthaqqrr  421 fdlsdlrykn gvdsylsvlt aqtdlytaqq alinarlarw tnlvdlyral gggwierage  481 tprapdqtvd ydkasapasa saaatng  818 19 161524257 YP_001579269 227 1 ttactccggc gaacccatca tcgactgctg atagttctgc aggccgacct tgccgatcag  61 gtcgatctgc gtttccagcc agtcgatatg ctcttcggta tcgtcgagga tcttttcgaa  121 gatctcgcgc gacacgtagt cgcgcaccga ttcgcaatag gcgatcgctt ccttgcaggt  181 ggcctgcgag atctgctcga gcttcaggtc gcacttcagg atttcttcgg tctcctcgcc  241 gacgagcagc ttgtgcagat cctgcaggtt cggcaggccg tcgagcatga acacgcgttc  301 gatcagccag tccgcgtgtt tcatttcgcc gatcgactcg tcgtattcgt gcttgccgag  361 cttgtcgagg ccccagtgct tgtacatgcg ggcatgcagg aagtactggt tgatcgccgt  421 gagttcgttc ttcagctggg cgttcaggta ttcgatgact ttcttgtcgc cttgcat  204 1 mqgdkkviey lnaqlknelt ainqyflhar mykhwgldkl gkheydesig emkhadwlie  61 rvfmldglpn lqdlhkllvg eeteeilkcd lkleqisqat ckeaiayces vrdyvsreif  121 ekilddteeh idwletqidl igkvglqnyq qsmmgspe  819 8.5 161524255 YP_001579267 228 1 tcaggccgcc ttgcgttcgt agaacgtcac cggaatcgga tgagcgtgat gctcgacgcc  61 gcaccggctc gcgcacacgc cgtgttcggc catcaggtcg cgtaccgact cctcgcattt  121 gccgcagcag gtcgccacgc caagctcgaa ctgaagctcg tcgaacgagt ccacgccctc  181 cgcgagggac gcgcgaatct tccgatcgga aacagacttg catacgcaga cgatcat  205 1 mivcvcksvs drkiraslae gvdsfdelqf elgvatccgk ceesvrdlma ehgvcasrcg  61 vehhahpipv tfyerkaa  820 36 161520970 YP_001584397 229 1 tcacttgaac cggatggtcg gattgatgac cgcgtagagc acgtcgacgg tcaggttgat  61 caggatgaat tcgagcgaga acagcagcac gatcgcctgg atcaccgggt aatcgcgcat  121 cgtcaccgaa tcgacgagca gccggccgag ccccggccag ttgaacacgg cctcgacgac  181 gatcgacccg ccgagcagga agccgaactg cagccccatc atcgtgacga ccggaatcat  241 cgcgttgcgc aggcagtgtt tgagcacgac cttcggctcg tgcacaccct tcgcgcgcgc  301 ggtgcgcacg aagtcctcgt tcatcacctc gacgaacgac gcgcgcgtga agcgcgccat  361 cacggccgcg accgccgcgc cgagcgtgag cgacggcagc acgtagctct tccacgtgtc  421 gtccggcacg acgggcagcc agccgagctt caccgagaag atctccatca agagcatgcc  481 gagcgcgaac gccggaaacg agatgccgga caccgcgagc gtcatgccga gccggtccgg  541 ccagcggttg cgccatacgg ccgacgcgat gccgatcgcc atcccgaaca gcgtcgccca  601 cgccatgctg acgatcgtca gcatcagcgt cggcatgaag cgctcgccga tttccgtcga  661 taccggccgc ttgctgcgcg tcgatacgcc gaaatcgccg tgtgcgatct tcacgaagaa  721 attcgcgaac tgcgtgggca gcggccgatc gaggccgaga tcggcgcgca cgagcgcgac  781 cgtcgcttcg tcggcctcgg ggccggccgc gagccgcgcc gggtcgcccg gcagcagatg  841 cacgaacaga aacacgagca ccgcgacgat cgcgagcgtg ggcagcaggc cgaacagccg  901 tttgatgatg aaagtcagca t  206 1 mltfiikrlf gllptlaiva vlvflfvhll pgdparlaag peadeatval vradlgldrp  61 lptqfanffv kiahgdfgvs trskrpvste igerfmptlm ltivsmawat lfgmaigias  121 avwrnrwpdr lgmtlavsgi sfpafalgml lmeifsvklg wlpvvpddtw ksyvlpsltl 181 gaavaavmar ftrasfvevm nedfvrtara kgvhepkvvl khclrnamip vvtmmglqfg  241 fllggsivve avfnwpglgr llydsvtmrd ypviqaivll fslefilinl tvdvlyavin  301 ptirfk  821 42 161520872 YP_001584299 230 1 tcagaagcgg tggatcatgc cgacgccgac ggcgatctgg ttctggccgg cacctgctgc  61 cacgccgtcg ccgatttgcg tcgttgccga ctggatcgcg ccagccttgc tcagcgtatt  121 gccgctcgcg tgctggtacg cctcgagtgc gtacaggccc gtgcgcttcg acaggctgta  181 gtactgcgac agcgtgacct ggtggtactt cgccgagctc gagatgccgt tcgacttcgt  241 tgccgccgtg tacgagtagc ccgccgcgaa gtcccactga gccgctgcct tccagtgcag  301 cacggcaccg gccgtgttga agatcgcggt gttgcggaac gacgagccga cgcccgggat  361 gtactggacg ttcgtgtagg acacggacac gtcccacgcc ggcgtgaact ggtagccggc  421 cgtcacgccg aggcgctgct gcgattgcgc cgttgcgtag ccgttgttga tcgccgacac  481 ggccggctgg ccgccgctcg tgaccgtcga gttgtcgccc cacgcgccgc cgccgagcgt  541 cgagttgttg acgcgctgat agccgaccgc gatgcctgcc gggccgttca ggtactggat  601 cgccgcgctc cacgtcgagc cgcggttcac gctgcccggc acgccgccga acgagtacga  661 gccgccgacc gtgaagccgt agaacttcgg cgacatgtag acgagcgaat tgttcgcgcg  721 gtagctcgta tcgagcgagt cgatatcacc cgggtgcgcg ccgtatgcgc ccgtcagcca  781 cgtcgtcggg ctgtacggcg acagcagcgt gtagtacgcc gtgtactggc gaccagccgt  841 cagcgtaccg tacgccgcgt tcgtcaggcc gacccacgcc tgacgcgtga agatgccgcc  901 cgaccactgc gatgcgcccg ttgccgtgtt cacgccggct tcgagctgga agatcgcctt  961 cgtgccgccg ccgaggtctt cgttgccctt caggccgaag cggctgcctg cccacacgcc  1021 ggtggacatc gacaccttcg aatgaccgcc gctcgtcgcg ccggtcgacg gtgccgcgtt  1081 attctggtac gccagaccgt tatcgacgat accgtacagg gtcacgctgc tctgagcgtg  1141 ggcggcggta gcggccgcaa ggccggctgc cgtcatggcg acgacgacgc gctttttcat  207 1 mkkrvvvamt aaglaaataa haqssvtlyg ivdnglayqn naapstgats gghskvsmst  61 gvwagsrfgl kgnedlgggt kaifqleagv ntatgasqws ggiftrqawv gltnaaygtl  121 tagrqytayy tllspysptt wltgaygahp gdidsldtsy rannslvyms pkfygftvgg  181 sysfggvpgs vnrgstwsaa iqylngpagi avgyqrvnns tlgggawgdn stvtsggqpa  241 vsainngyat aqsqqrlgvt agyqftpawd vsvsytnvqy ipgvgssfrn taifntagav  301 lhwkaaaqwd faagysytaa tksngisssa kyhqvtlsqy yslskrtgly aleayqhasg  361 ntlskagaiq sattqigdgv aagagqnqia vgvgmihrf  822 56 161524923 YP_001579935 231 1 atgaaatcct ccccgttgtc cgtgcgtgcc gggtcgcgcc gcaccgccgt tgccgttgcg  61 gtcgccgcac tggcactggc gggctgcgcg aactacatcg gcatcaagag cgacaagcag  121 atcgcccccg cgtcgcagtt cgaaaccgcg cagagccttc cggcccaggg cggccactgg  181 ccgtcgctcg actgggcgag ccagttcggc gatccgcagc tgccgaagct gatcgacgag  241 gcgctcgccg gcaatccgtc gatcgcgcag gcgcaggcgc gcatcgcgaa ggcgtcgtcg  301 tatatcgaat cgtcgcgctc gaacctgctg ccgaaggccg aagcgagcta ctcgtggacg  361 cgcgagctct attcggcgaa cggcctcttc ccgccgccgt acggcggcca gtggtacagc  421 gagaacaacg tgctcgccag cgcatcgtgg gagctcgacc tgtggggcaa gaaccgcgag  481 cgcctgcgca cggccgtgtc gcaggaaaag gccgcggaag ccgacatgca gcaggcgcgc  541 atcacgctcg cctcgtcggt cgcgcgcacc tacaactcgc tcgcgcagct gtacgcgctg  601 cgcgacatcg cgcagcgcga gatcgccaac cgcgagtcgg tcggcaagat caccgacggc  661 cgcgtgtcgg ccggcctcga cacgaacgtc gaacgccaga ccgcacgcgg caacatcgcg  721 acgacgcagg cgtcgctgtc cgatctcgac ggtcagatca cgaccgtgcg ctaccagctc  781 gccgcactgc tcggcaaagg gccggaccgc gggctgcaga tcgccgcgcc ggtgctgaac  841 ccggccggcg acgtcgcgct acccgacaac ctgcccgccg acctcgtatc gcgccgcccc  901 gacatcgtcg ccgcgcgctg gcaggtcgaa gcggcaatgc acgacgtgaa ggaagcgaag  961 gccgagttct tcccggacgt gaacctcgcg gccggtttcg gtttcgatgc gttcggctgg  1021 ggcaaattcc tgaacttcac gagccgccag gcgcagttcg gcccggcgat ccatctgccg  1081 atcttcgacg ccggcgcgct gcgcgcgcag ctcaagggcc gctacgcgga cttcgatctg  1141 tcggtcgcga actacaacca gacgctgatc agcgcgctga acgacgtggc gacgcaagtt  1201 gccgcgatcc gtgcgatcga tcgccagatg ggcgacgcgc agcgcgcgct cgacgcgtcg  1261 acgcgcgcat acgatctcgc ggtgattcgc tacaaggccg gcctgtcgcc gcagctgcag  1321 gtgctgaccg cggacagcaa ccggctcgcc tcggagcaga ccgtgacgaa tctgaagatg  1381 cgccgtcgcg acatgcagct cgcgctgatc aaggcgctcg gcggcggctt cgatgcgacc  1441 ggcaccgcgc tcgcggcgcc cgagaccgcg aaacagaccc gacaggccgc caactga  208 1 mkssplsvra gsrrtavava vaalalagca nyigiksdkq iapasqfeta qslpaqgghw  61 psldwasqfg dpqlpklide alagnpsiaq aqariakass yiessrsnll pkaeasyswt  121 relysanglf pppyggqwys ennvlasasw eldlwgknre rlrtavsqek aaeadmqqar  181 itlassvart ynslaqlyal rdiaqreian resvgkitdg rvsagldtnv erqtargnia  241 ttqaslsdld gqittvryql aallgkgpdr glqiaapvln pagdvalpdn lpadlvsrrp  301 divaarwqve aamhdvkeak aeffpdvnla agfgfdafgw gkflnftsrq aqfgpaihlp  361 ifdagalraq lkgryadfdl svanynqtli salndvatqv aairaidrqm gdaqraldas  421 traydlavir ykaglspqlq vltadsnrla seqtvtnlkm rrrdmqlali kalgggfdat  481 gtalaapeta kqtrqaan  823 85 161525558 YP_001580570 232 1 tcacagcgac catttcagtt ctccatagaa agtgcgaccc ggatacgggt ggaacacgta  61 gtagcggcgg tccgtcaggt tgtcgatgcc aaccgatgcg gtccagtgac gatcgaagcg  121 atagcgcgcc ttcacgtcga cgaccgtaaa cgcgctcgtg ccgccataga cgtcgggatt  181 cacgtcgctg ttgtcgagcg tgttgaactg ccggcccgaa tagcgcacgc cgacgctcgc  241 gagccagtgc tcgtcgaagc gatacgacgc gagcagattc gcgcgcatcc gcggaatgcg  301 cggaaagcgc gagccgacgt acgccgggtt ggcggcatcg gcgagaatct gcgcattgct  361 cgccgatacg ttcgcgtcga tcgcgagccc cttgagcccg acgttttcgc cgctgaacgc  421 gagttcgacg ccgcgcacgc gcacgcggtc gacgttcgag atgttggtga ccgtcgaggc  481 gccggcgacc gtcgtctggc tgtagatgga atcgcgcaag tcgctctgga acacgctcgc  541 acgcacgacg ccgacgccga cgtcgcgctc cgcggtgaag tcccagtcga tcgccttttc  601 ggggcgcagg ttcggattgt tgttgacgat cgcgttgttc gagatcgtcc cctggaacag  661 ctcgccgacc gtcggaaaac gcgtgccggt cgcgaacgac agccggaagc gccacacgtc  721 ggtcgcatcc cattgcagcg caactttcgg cgacagcgcg ttggcgctgc gatccgcata  781 gccgaacgtg ccgctcgcgt tgccgagcgc gccgccgtag gcgtcccagc gctcgtagcg  841 cagcccgagc gtcgcgagcc agcccggcgc gaagcgccat gcgtcctgcg cgtagagcgc  901 ctgcgtgcgc gtatcgccgc gatagacgct ggcgagcgac gtgaccggac cgttcagcca  961 gtcggccgtg ttgtatgtga cgttgcgcag gaaatagttg tcgtagtgat agccgaacgt  1021 gaacgcatga ccgtgcacct cgggcgcttc ggccttcagg tcgagcgtgc gccagccggt  1081 gccgtcaccc tggaacagcg tgcccgcccc gccctgcgcg gtagacgcgg cgcgcagcac  1141 gtcgcgcgac acgtcgtacg ccgacacgat gcccgacagc cgccagcccg acgcgagccg  1201 gccgttcagc ccgaacgcgt acagccagtt ctcctggtcg ccgcgctgcg gcgcgaatgc  1261 gccgggcgcg atcgtcaggt tctgcccgcc gatcgccacg ttgccgccat agaccggctg  1321 gccggccgcg tcgcgcagaa acgtgtcgcc gtgctggcgg tagtggttct cccaatgccc  1381 gagcgtcagc gtcgcgtcga cgcggtcggt gaacgcatag cccatgcgca ccgtttcgtt  1441 gatctgctcg gtgcgctcga gcgattgcgc gccgacgatc gtgcgcgcct tgccgttcgg  1501 cccgatgtcg gtggcggcgc ccgtgacggg caccgcgcgg ccgagtttcg cgttgtagct  1561 cgcgttcggg ctcgcgtact gcatcggctg accgtcgttc tcgagccggt cgagcgacag  1621 cgagaaccag aagcggccga tgcggttcgc gatccgcgca gtctgatgat tgccgccgaa  1681 gctgtcggcg aatccgtagc cgtcgcgata gcgctgcgtg aagaactgcg tcgacagcga  1741 cgcctcgagc ttgtccggcc gccgcgtggt cagtagcacg gtcgagccga tcgcattgcc  1801 cggatacagc gccgaaaacg ggccgtacag cacgtcgacg cgcgcgatgt cgtcgggcgc  1861 gatcagcgac cagcgcggcg gatacgcata gctcgaaccg agcaggttcg acaacagcag  1921 gccgtccgca tagacgaggc cccgcgcgct ctgcagctcg ttgaagtcgc gccccgcgaa  1981 cacgctgttg cggtcgccga tatagcgctt gcgcaccatc aggttcggcg cgtacttcag  2041 cgtgtcctcg gccgtgacgt tggtatgcgt gtcgatgcgc tcgcgcgtca tcgattcgac  2101 gacggccggc gtatcgggat cgagcggctg gcgctgggcc gtcacgctga cggcgctcag  2161 cgtgtcgccg gccggttcgg cggaagcagc cgaagccgcg gaagccgcgg gcacgacggc  2221 ccgcgcggag cggttcgcgg gatcgcggac cggatcggtc ggcgcgctgt cggacgatgc  2281 cgcatatgcg ccgggcaagg caaaagcggc cgcgcacgcc agcgcgagcc ggccgcgcgt  2341 cggtcgcgcg gcaagggtaa gcaacat  209 1 mlltlaarpt rgrlalacaa afalpgayaa ssdsaptdpv rdpanrsara vvpaasaasa  61 asaepagdtl savsvtaqrq pldpdtpavv esmtreridt htnvtaedtl kyapnlmvrk  121 ryigdrnsvf agrdfnelqs arglvyadgl llsnllgssy aypprwslia pddiarvdvl  181 ygpfsalypg naigstvllt trrpdkleas lstqfftqry rdgygfadsf ggnhqtaria  241 nrigrfwfsl sldrlendgq pmqyaspnas ynaklgravp vtgaatdigp ngkartivga  301 qslerteqin etvrmgyaft drvdatltlg hwenhyrqhg dtflrdaagq pvyggnvaig  361 gqnitiapga fapqrgdqen wlyafglngr lasgwrlsgi vsaydvsrdv lraastaqgg  421 agtlfqgdgt gwrtldlkae apevhghaft fgyhydnyfl rnvtyntadw lngpvtslas  481 vyrgdtrtqa lyaqdawrfa pgwlatlglr yerwdaygga lgnasgtfgy adrsanalsp  541 kvalqwdatd vwrfrlsfat gtrfptvgel fqgtisnnai vnnnpnlrpe kaidwdftae  601 rdvgvgvvra svfqsdlrds iysqttvaga stvtnisnvd rvrvrgvela fsgenvglkg  661 laidanvsas naqiladaan payvgsrfpr iprmranlla syrfdehwla svgvrysgrq  721 fntldnsdvn pdvyggtsaf tvvdvkaryr fdrhwtasvg idnltdrryy vfhpypgrtf  781 ygelkwsl  824 78 161520731 YP_001584158 233 1 tcagaacgtc gtgcgcaatc cggccatcac gctgcggccg ccttgcggcg cgaagccgcg  61 cacgacggag gtcgcgtagc ggatgtcctg gttcgtcagg ttgtcgccgc gcagatacgc  121 gagccagtgc gtcgcgccga cgcggaactt gtacgtcagc accacgccga gcgaggtata  181 gccggcggtc ggttcatcgt cggcgggcac gcgatgctgc gaccacgcgt gcgtgagctg  241 cgcgcgtgcg ccgaacggac cgtagccgta atcggccgcg agcgtcgcgc gcagcggtgc  301 gatgcgcggc agcggctcgc cggtgtcgac gttgcgcgca tgcgtatagt cggccgtcaa  361 ctcgaggtcg accgtgtggc cgctgtgtgc atagacgcga tacttgccgt cgagctcgat  421 gccgtagaac tccgcgcgca cgccgcgata gacggcctcg gcgagcgcgt cgtcggtgcc  481 gcgcgggacc ggctcgccgt cgctgtcgac gatgcgcccg gtcgcgtatt cggccagata  541 gttcgaaaag cggttgtaga acatgccgac gctgccgcga ttcggtccgc tcgcatagcg  601 cagcgacaga tcggtcgaca cggccttttc cttcgacgca ttcggattgc cgatcaggta  661 ctgcccggtc gcatcgtgcg ggccgttcga atagagctcg tagaacgtgg gcgcgcgttc  721 ggtgtacgcg acgttcgcgg cgatcgacca gacgggcgtc agcgaaaaca gcgcgccggc  781 cgacacgctg cctgcgttga acgtgcgcga ttgcgcgccc gcgaatttct cgacgccggc  841 cggatcggga tcgacgttca catgctcgat gcgtccgccg aggctcagct tcagcgccgg  901 atcgacctgc cattcctcga ggccgaatag cgcgacgcta ttggtgcgcg tcgacggaac  961 cagcgtttcg tcgccgagcg cggagaacgt attgccgccg aactgcacgc cgatcgcgcc  1021 ttcgagcggc ccaagcttgc ggtgccgcgc ctcgatgcgc gcctcgtagc cgcgattgcg  1081 gaacgtcgtc gcggtttcgc cgttgtcgac ttccttgtgg cggtaatcgg tgtaggcgaa  1141 gtcgaatttc agcgtcgaga acggccccgg caggttgcgc gcctccgacg cgaaggcgag  1201 gcgctcctga cgcatgcgca gccgcacgtc gtcttcggcg accgacccgt agttcgattc  1261 gtagccgctg tacgacaggc ccgcgaaacc gtcggcccac gtgtacgacg cgccgaccgc  1321 gccgccgtgc acgcggccgt cgctgttcgg cacgctgccg accggctgcg gcgtgtcggg  1381 gccgtcgagc gcgcgttgcg tatcgctgcg cgcatagccg ggaatccgca gcttgccggt  1441 ttcgcgatcg aatgcgtcga cgtggaacgc gaagcggcca ttgccgcctt cgacctgcgc  1501 ggcgcccgcg cgcgtggcgt tcgcgccgcc gtagcggacg tcgagcgtac cggtcggacc  1561 gtcgatcgcc tcgcgcggga tccggttgtc gatcgtgttc acgacgccgc cgatcgcgtt  1621 gccgccgtac agcaacgccg ccgggccgcg cacgatctcg acgcgctcga tcgacagcgg  1681 gtcttccggc acggcatggt cgtacgaaag cgacgacgca tcgtaggcgg ccacgccgtt  1741 ctgcagcagc cggatccggt cgccgtccat gccgcgcacg atcgggcggc cgaccatcgg  1801 cccgtacgtc gtggtcgaca cgccgggcag gccgttcagc gtttcgccga gcgaatcggc  1861 ctggcggcgc agcagcgcat cgcccgacag ctgcgcggtg ggcgcgatca gttcggcgtc  1921 gccgagcgga ttcgcggtga cgaaaatcgg cgcgagcggc gcgccgctcg aggacggcga  1981 cgtcgcggtt gcgggcgtgt cggtttgcgc atgcgcaagg gcggcgagcg ccatcagcga  2041 aacgggcgag agcgggcgga gcggaagacg agggagacgt ggaagacgtg ggaggtcgcg  2101 catggtcgag gagtcggttg aagtctgtcg gaatcggatc gggggcgagc ccggcgcgcc  2161 gcgaatcggt ttcat  210 1 mkpirgapgs ppirfrqtst dsstmrdlpr lprlprlplr plspvslmal aalahaqtdt  61 patatspsss gaplapifvt anplgdaeli aptaqlsgda llrrqadslg etlnglpgvs  121 tttygpmvgr pivrgmdgdr irllqngvaa ydasslsydh avpedplsie rveivrgpaa  181 llyggnaigg vvntidnrip reaidgptgt ldvryggana tragaaqveg gngrfafhvd  241 afdretgklr ipgyarsdtq raldgpdtpq pvgsvpnsdg rvhggavgas ytwadgfagl  301 sysgyesnyg svaeddvrlr mrqerlafas earnlpgpfs tlkfdfaytd yrhkevdnge  361 tattfrnrgy eariearhrk lgplegaigv qfggntfsal gdetlvpstr tnsvalfgle  421 ewqvdpalkl slggriehvn vdpdpagvek fagaqsrtfn agsvsagalf sltpvwsiaa  481 nvayterapt fyelysngph datgqylign pnaskekavs tdlslryasg pnrgsvgmfy  541 nrfsnylaey atgrivdsdg epvprgtdda laeavyrgvr aefygieldg kyrvyahsgh  601 tvdleltady tharnvdtge plpriaplra tlaadygygp fgaraqltha wsqhrvpadd  661 eptagytslg vvltykfrvg athwlaylrg dnltnqdiry atsvvrgfap qggrsvmagl  721 rttf  825 43 161523868 YP_001578880 234 1 atgcgcgtcg aacgggttcc ataccgctta ctcactgtcg cgacggccgc cgttttcctg  61 gccgcgtgcg ggaaaaaaga atcggcaccg ccgccgcaaa cgccggaagt cggcgtcgtc  121 acggtccagc cgcaggccgt accggtcttc accgaactgc ctggccgcac cagcgcgttc  181 ctcgtcgcgc aggtccgcgc gcgggtcgac ggcatcgtgc tgcgccgtga attcaccgaa  241 ggcagcgacg tcaaggccgg tcagcgcctc tacaagatcg acccggcacc gtatatcgcc  301 gcgctgaaca gcgcgaaggc gacgctcgcg aaggcgcagg cgaacctcgc cacgcagaac  361 gcgctcgtcg cgcgctacaa ggtgctggtg gccgcgaacg cggtcagcaa gcaggactac  421 gacaacgcgg tggccgcgca agggcaggcc gccgccgacg tcgcggccgg caaggcgtcg  481 gtcgagaccg cgcagatcaa cctcggctac acggacgtcg tatcgccgat caccggccgc  541 gtcggcatct cgcaggtgac gccgggcgcg tacgtgcagg cgagccaggc gacgctgatg  601 tcgaccgtgc agcagctcga cccggtctac gtcgacctca cgcaatcgag cctcgagggg  661 ctgaagctgc gtcaggacgt gcagagcggc cgtctgaaga cgacgggccc cggtgccgcg  721 aaggtgtcgc tgatcctgga ggacggccgc acctattcgc agcccggcaa gctgcagttc  781 tccgacgtga cggtcgacca gacgaccggc tcggtcacga tccgtgcggt gttcccgaac  841 ccgggcaagg tcctgctgcc ggggatgttc gtgcgcgcgc gcatcgagga aggcgtgaac  901 gacaacgcgt atctggtgcc gcagatcggc gtcacgcacg accagaaggg ccaggcggtc  961 gcgatggtcg tcaacgcgaa caacaaggtc gagccgcgca cgctgaccgc aacgcgcatc  1021 gaaggtcaga actgggtcgt cgaaagcggg ctgcagcccg gcgatcgcgt gatcgtgcag  1081 ggtgtcgaca aggtgcgtcc gggcgcgacc gtgaaggccg ttccggcgca gctcgcgtcg  1141 gcgccgaacg acgcgtcggg tgccgccgcg ccggccgccg ccgcaccggc ttcggccgcc  1201 gcggcatcgg ccgccgcagc atcgagtgcc ggcgcgtcgg gcgcgccggc tagcgccgcc  1261 gcggggtcgt ccgccgccgc ttcgggcgcg caataa  211 1 mrvervpyrl ltvataavfl aacgkkesap ppqtpevgvv tvqpqavpvf telpgrtsaf  61 lvaqvrarvd givlrrefte gsdvkagqrl ykidpapyia alnsakatla kaqanlatqn  121 alvarykvlv aanayskqdy dnavaaqgqa aadvaagkas vetaqinlgy tdvvspitgr  181 vgisqvtpga yvqasqatlm stvqqldpvy vdltqssleg lklrqdvqsg rlkttgpgaa  241 kvsliledgr tysqpgklqf sdvtvdqttg svtiravfpn pgkvllpgmf vrarieegvn  301 dnaylvpqig vthdqkgqav amvvnannkv eprtltatri egqnwvvesg lqpgdrvivq  361 gvdkvrpgat vkavpaqlas apndasgaaa paaaapasaa aasaaaassa gasgapasaa  421 agssaaasga q  *This ID number is an internal laboratory designation that was used to identify the polypeptides in U.S. Provisional Patent Application Ser. No. 61/922,504, filed Dec. 31, 2013.

The complete disclosure of all patents, patent applications, and publications, and electronically available material (including, for instance, nucleotide sequence submissions in, e.g., GenBank and RefSeq, and amino acid sequence submissions in, e.g., SwissProt, PIR, PRF, PDB, and translations from annotated coding regions in GenBank and RefSeq) cited herein are incorporated by reference. In the event that any inconsistency exists between the disclosure of the present application and the disclosure(s) of any document incorporated herein by reference, the disclosure of the present application shall govern. The foregoing detailed description and examples have been given for clarity of understanding only. No unnecessary limitations are to be understood therefrom. The invention is not limited to the exact details shown and described, for variations obvious to one skilled in the art will be included within the invention defined by the claims.

Unless otherwise indicated, all numbers expressing quantities of components, molecular weights, and so forth used in the specification and claims are to be understood as being modified in all instances by the term “about.” Accordingly, unless otherwise indicated to the contrary, the numerical parameters set forth in the specification and claims are approximations that may vary depending upon the desired properties sought to be obtained by the present invention. At the very least, and not as an attempt to limit the doctrine of equivalents to the scope of the claims, each numerical parameter should at least be construed in light of the number of reported significant digits and by applying ordinary rounding techniques.

Notwithstanding that the numerical ranges and parameters setting forth the broad scope of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as possible. All numerical values, however, inherently contain a range necessarily resulting from the standard deviation found in their respective testing measurements.

All headings are for the convenience of the reader and should not be used to limit the meaning of the text that follows the heading, unless so specified. 

What is claimed is:
 1. A composition comprising: at least two isolated polypeptides that are involved in iron acquisition by a Burkholderia spp., wherein each polypeptide is isolatable from a Burkholderia spp. when the Burkholderia spp. is grown under conditions effective for the Burkholderia spp. to express the polypeptide.
 2. The composition of claim 1 wherein at least one of the isolated polypeptides comprises the amino acid sequence of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, or an immunogenic fragment or structurally similar variant thereof.
 3. The composition of claim 1 wherein at least one of the isolated polypeptides comprises the amino acid sequence of SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, SEQ ID NO:60, SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ ID NO:70, SEQ ID NO:71, SEQ ID NO:72, SEQ ID NO:73, SEQ ID NO:74, SEQ ID NO:75, or an immunogenic fragment or structurally similar variant thereof.
 4. The composition of claim 1 wherein at least one of the isolated polypeptides comprises the amino acid sequence of SEQ ID NO:101, SEQ ID NO:102, SEQ ID NO:103, SEQ ID NO:104, SEQ ID NO:105, SEQ ID NO:106, SEQ ID NO:107, SEQ ID NO:108, SEQ ID NO:109, SEQ ID NO:110, SEQ ID NO:111, SEQ ID NO:112, SEQ ID NO:113, SEQ ID NO:114, SEQ ID NO:115, SEQ ID NO:116, SEQ ID NO:117, SEQ ID NO:118, SEQ ID NO:119, SEQ ID NO:120, or an immunogenic fragment or structurally similar variant thereof.
 5. The composition of claim 1 wherein at least one of the isolated polypeptides comprises the amino acid sequence of SEQ ID NO:141, SEQ ID NO:142, SEQ ID NO:143, SEQ ID NO:144, SEQ ID NO:145, SEQ ID NO:146, SEQ ID NO:147, SEQ ID NO:148, SEQ ID NO:149, SEQ ID NO:150, SEQ ID NO:151, SEQ ID NO:152, SEQ ID NO:153, SEQ ID NO:154, SEQ ID NO:155, SEQ ID NO:156, SEQ ID NO:157, SEQ ID NO:158, SEQ ID NO:159, SEQ ID NO:160, SEQ ID NO:161, SEQ ID NO:162, SEQ ID NO:163, SEQ ID NO:164, or an immunogenic fragment or structurally similar variant thereof.
 6. The composition of claim 1 wherein at least one of the isolated polypeptides comprises the amino acid sequence of SEQ ID NO:189, SEQ ID NO:190, SEQ ID NO:191, SEQ ID NO:192, SEQ ID NO:193, SEQ ID NO:194, SEQ ID NO:195, SEQ ID NO:196, SEQ ID NO:197, SEQ ID NO:198, SEQ ID NO:199, SEQ ID NO:200, SEQ ID NO:201, SEQ ID NO:202, SEQ ID NO:203, SEQ ID NO:204, SEQ ID NO:205, SEQ ID NO:206, SEQ ID NO:207, SEQ ID NO:208, SEQ ID NO:209, SEQ ID NO:210, SEQ ID NO:211, or an immunogenic fragment or structurally similar variant thereof.
 7. A method comprising: administering to a subject an amount of a composition effective to induce the subject to produce antibody that specifically binds to at least one component of the composition, wherein the composition comprises: at least two isolated polypeptides that are involved in iron acquisition by a Burkholderia spp., wherein each polypeptide is isolatable from a Burkholderia spp. when the Burkholderia spp. is grown under conditions effective for the Burkholderia spp. to express the polypeptide.
 8. A method for treating an infection in a subject, the method comprising: administering an effective amount of a composition to a subject having or at risk of having an infection caused by a Burkholderia spp., wherein the composition comprises: at least two isolated polypeptides that are involved in iron acquisition by a Burkholderia spp., wherein each polypeptide is isolatable from a Burkholderia spp. when the Burkholderia spp. is grown under conditions effective for the Burkholderia spp. to express the polypeptide.
 9. A method for treating a symptom in a subject, the method comprising: administering an effective amount of a composition to a subject having or at risk of having an infection caused by a Burkholderia spp., wherein the composition comprises: at least two isolated polypeptides that are involved in iron acquisition by a Burkholderia spp., wherein each polypeptide is isolatable from a Burkholderia spp. when the Burkholderia spp. is grown under conditions effective for the Burkholderia spp. to express the polypeptide.
 10. A method for decreasing colonization in a subject, the method comprising: administering an effective amount of a composition to a subject colonized by a Burkholderia spp., wherein the composition comprises: at least two isolated polypeptides that are involved in iron acquisition by a Burkholderia spp., wherein each polypeptide is isolatable from a Burkholderia spp. when the Burkholderia spp. is grown under conditions effective for the Burkholderia spp. to express the polypeptide.
 11. A method for treating an infection in a subject, the method comprising: administering an effective amount of a composition to a subject having or at risk of having an infection caused by a Burkholderia spp., wherein the composition comprises: antibody that specifically binds at least two isolated polypeptides that are involved in iron acquisition by a Burkholderia spp., wherein each polypeptide is isolatable from a Burkholderia spp. when the Burkholderia spp. is grown under conditions effective for the Burkholderia spp. to express the polypeptide.
 12. A method for treating a symptom in a subject comprising: administering an effective amount of a composition to a subject having or at risk of having an infection caused by a Burkholderia spp., wherein the composition comprises: antibody that specifically binds at least two isolated polypeptides that are involved in iron acquisition by a Burkholderia spp., wherein each polypeptide is isolatable from a Burkholderia spp. when the Burkholderia spp. is grown under conditions effective for the Burkholderia spp. to express the polypeptide.
 13. A method for decreasing colonization in a subject, the method comprising: administering an effective amount of a composition to a subject colonized by a Burkholderia spp., wherein the composition comprises: antibody that specifically binds at least two isolated polypeptides that are involved in iron acquisition by a Burkholderia spp., wherein each polypeptide is isolatable from a Burkholderia spp. when the Burkholderia spp. is grown under conditions effective for the Burkholderia spp. to express the polypeptide.
 14. A kit for detecting antibody that specifically binds a polypeptide, comprising in separate containers: an isolated polypeptide involved in iron acquisition by a Burkholderia spp., wherein the polypeptide is isolatable from a Burkholderia spp. when the Burkholderia spp. is grown under conditions effective for the Burkholderia spp. to express the polypeptide; and a reagent that detects an antibody that specifically binds the polypeptide.
 15. A kit for detecting a polypeptide, comprising in separate containers: an antibody that specifically binds an isolated polypeptide involved in iron acquisition by a Burkholderia spp., wherein the polypeptide is isolatable from a Burkholderia spp. when the Burkholderia spp. is grown under conditions effective for the Burkholderia spp. to express the polypeptide; and a second reagent that specifically binds the polypeptide.
 16. A composition comprising: isolated antibody that specifically binds to a polypeptide involved in iron acquisition by a Burkholderia spp., wherein the polypeptide is isolatable from a Burkholderia spp. when the Burkholderia spp. is grown under conditions effective for the Burkholderia spp. to express the polypeptide.
 17. A composition comprising: an isolated whole cell that comprises a polypeptide involved in iron acquisition by a Burkholderia spp., wherein the polypeptide is isolatable from a Burkholderia spp. when the Burkholderia spp. is grown under conditions effective for the Burkholderia spp. to express the polypeptide.
 18. A composition comprising: isolated antibody that specifically binds to a whole cell that comprises a polypeptide involved in iron acquisition by a Burkholderia spp., wherein the polypeptide is isolatable from a Burkholderia spp. when the Burkholderia spp. is grown under conditions effective for the Burkholderia spp. to express the polypeptide.
 19. A recombinant cell comprising increased expression of a Burkholderia spp. metal-regulated polypeptide compared to a wild-type control. 